BuceA.18444.a

Putative glycosyltransferase

CENTER ID: BuceA.18444.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18444.a.B1.GE35024 full length 1 354
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2514
RefSeq: YP_002231531.1
UniProt: B4E6D3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIGLSANAL KHGGGLERYA IDIARGMAEA GVTPAFFARS VDASIPEARL VEPHRINVSF LPGKVRDEWF SWRLRGARRA ADVDVLIGCN RVACSEIAIC GGTHRGFLRA IGRAPGYFDR RQIALESRQY ANARYVVAHS QMMRDELRTL YGVDDARIRM LYPPVDAQRF TPVGDDERTA LRRRFGFGER DVVLLFPSSS HVRKGLPLIE AALQGTDASI VVAVAGRPPE RTSAQLRYIG YVKDIEDGYR AADYTILASK YEPFGLVGIE SVMCGTPVIL PPTLGCHDVI ADHAKLTFAA DDVEALRAVL ATAAQRVRAG DRAAVARDAV GYDPGVRHHV EQLLALAREA WAPA
NT Sequence
atgaagatcg gcctgtcggc caatgcgctg aagcacgggg gcggcctcga gcgctacgcg atcgatatcg cgcggggaat ggcggaggcg ggcgtgacgc ccgcgttctt cgcgcgctcc gtcgacgcgt cgattccgga ggcgcggctc gtcgagccgc atcggatcaa cgtctcgttc ctgcccggca aggtgcgcga cgaatggttc tcgtggcggc tccgcggtgc acgccgcgcg gccgacgtgg atgtgctgat cggctgtaat cgggtcgcct gctcggagat cgcgatttgc ggcggcacac atcgagggtt cttgcgcgcg atcggccgcg caccgggcta tttcgaccgg cgccagattg cactcgagag ccggcagtac gcgaatgcgc gctatgtggt cgcgcattcg cagatgatgc gcgacgaact gcgcacgctg tacggcgtcg acgacgccag gatccgcatg ctgtatcccc ccgtcgatgc gcagcgcttc acgcctgtcg gcgacgacga gcgcaccgcg ttgcggcgcc gcttcggttt cggcgagcgc gacgtcgtgc tgctgtttcc gtcgagcagc cacgtgcgca agggcttgcc gctgatcgag gccgcgctgc aggggacgga cgcgtcgatc gtcgtcgcgg tggccggccg cccgcccgag cggacctcgg cgcaactgcg ctacatcggc tacgtgaagg acatcgagga cggataccgc gcggcggatt acacgatcct tgcatcgaaa tacgagccgt tcgggctggt cggcatcgag tcagtgatgt gcgggacgcc cgtgatcttg ccgcccacgc tcggctgcca tgacgtcatt gcggaccatg cgaagctgac gttcgcggcc gacgatgtcg aggcgctgcg cgccgtgctg gcgacggccg cacagcgcgt gcgagccggc gaccgggccg ccgtcgcgcg cgacgcggta ggctacgacc cgggcgtgcg ccatcatgtc gagcagttgc tcgcgctggc ccgcgaggcg tgggcgccgg cgtag
Details for BuceA.18444.a.B1.GE35024
HARVESTED ON: 3/19/2012
SEQUENCED ON: 3/22/2012
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMK IGLSANALKH GGGLERYAID IARGMAEAGV TPAFFARSVD ASIPEARLVE PHRINVSFLP GKVRDEWFSW RLRGARRAAD VDVLIGCNRV ACSEIAICGG THRGFLRAIG RAPGYFDRRQ IALESRQYAN ARYVVAHSQM MRDELRTLYG VDDARIRMLY PPVDAQRFTP VGDDERTALR RRFGFGERDV VLLFPSSSHV RKGLPLIEAA LQGTDASIVV AVAGRPPERT SAQLRYIGYV KDIEDGYRAA DYTILASKYE PFGLVGIESV MCGTPVIXPP XXXCXDVIAD HAKLXFAAXD VXALRAVLAX XTARXSXRXX XXXGXXXLXX XVXXMXXXXX XPXXXXXXXX X
Validated NT Sequence
tttntttnct ttaagaagga ganataccat ggctcaccac caccaccacc atatgaagat cggcctgtcg gccaatgcgc tgaagcacgg gggcggcctc gagcgctacg cgatcgatat cgcgcgggga atggcggagg cgggcgtgac gcccgcgttc ttcgcgcgct ccgtcgacgc gtcgattccg gaggcgcggc tcgtcgagcc gcatcggatc aacgtctcgt tcctgcccgg caaggtgcgc gacgaatggt tctcgtggcg gctccgcggt gcacgccgcg cggccgacgt ggatgtgctg atcggctgta atcgggtcgc ctgctcggag atcgcgattt gcggcggcac acatcgaggg ttcttgcgcg cgatcggccg cgcaccgggc tatttcgacc ggcgccagat tgcactcgag agccggcagt acgcgaatgc gcgctatgtg gtcgcgcatt cgcagatgat gcgcgacgaa ctgcgcacgc tgtacggcgt cgacgacgcc aggatccgca tgctgtatcc ccccgtcgat gcgcagcgct tcacgcctgt cggcgacgac gagcgcaccg cgttgcggcg ccgcttcggt ttcggcgagc gcgacgtcgt gctgctgttt ccgtcgagca gccacgtgcg caagggcttg ccgctgatcg aggccgcgct gcaggggacg gacgcgtcga tcgtcgtcgc ggtggccggc cgcccgcccg agcggacctc ggcgcaactg cgctacatcg gctacgtgaa ggacatcgag gacggatacc gcgcggcgga ttacacgatc cttgcatcga aatacgagcc gttcgggctg gtcggcatcg agtcagtgat gtgcgggacg cccgtgatcn tgccgcccnc nnncngctgc cntgacgtca ttgcggacca tgcgaagctg angttcgcgg ccgangatgt cgangcgctg cgcgccgtgc tggcgangnn nacagcgcgt gngagcnggc gannngnnnn nncncncgga cnnngnnngc tacnacnngn ngtgnnnntc atgtnnnnnn nnnctnntnn nccnnnnngg nnnngggnnn cnnnnntann nnnt
Expected Protein Sequence
MAHHHHHHMK IGLSANALKH GGGLERYAID IARGMAEAGV TPAFFARSVD ASIPEARLVE PHRINVSFLP GKVRDEWFSW RLRGARRAAD VDVLIGCNRV ACSEIAICGG THRGFLRAIG RAPGYFDRRQ IALESRQYAN ARYVVAHSQM MRDELRTLYG VDDARIRMLY PPVDAQRFTP VGDDERTALR RRFGFGERDV VLLFPSSSHV RKGLPLIEAA LQGTDASIVV AVAGRPPERT SAQLRYIGYV KDIEDGYRAA DYTILASKYE PFGLVGIESV MCGTPVILPP TLGCHDVIAD HAKLTFAADD VEALRAVLAT AAQRVRAGDR AAVARDAVGY DPGVRHHVEQ LLALAREAWA PA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga tcggcctgtc ggccaatgcg ctgaagcacg ggggcggcct cgagcgctac gcgatcgata tcgcgcgggg aatggcggag gcgggcgtga cgcccgcgtt cttcgcgcgc tccgtcgacg cgtcgattcc ggaggcgcgg ctcgtcgagc cgcatcggat caacgtctcg ttcctgcccg gcaaggtgcg cgacgaatgg ttctcgtggc ggctccgcgg tgcacgccgc gcggccgacg tggatgtgct gatcggctgt aatcgggtcg cctgctcgga gatcgcgatt tgcggcggca cacatcgagg gttcttgcgc gcgatcggcc gcgcaccggg ctatttcgac cggcgccaga ttgcactcga gagccggcag tacgcgaatg cgcgctatgt ggtcgcgcat tcgcagatga tgcgcgacga actgcgcacg ctgtacggcg tcgacgacgc caggatccgc atgctgtatc cccccgtcga tgcgcagcgc ttcacgcctg tcggcgacga cgagcgcacc gcgttgcggc gccgcttcgg tttcggcgag cgcgacgtcg tgctgctgtt tccgtcgagc agccacgtgc gcaagggctt gccgctgatc gaggccgcgc tgcaggggac ggacgcgtcg atcgtcgtcg cggtggccgg ccgcccgccc gagcggacct cggcgcaact gcgctacatc ggctacgtga aggacatcga ggacggatac cgcgcggcgg attacacgat ccttgcatcg aaatacgagc cgttcgggct ggtcggcatc gagtcagtga tgtgcgggac gcccgtgatc ttgccgccca cgctcggctg ccatgacgtc attgcggacc atgcgaagct gacgttcgcg gccgacgatg tcgaggcgct gcgcgccgtg ctggcgacgg ccgcacagcg cgtgcgagcc ggcgaccggg ccgccgtcgc gcgcgacgcg gtaggctacg acccgggcgt gcgccatcat gtcgagcagt tgctcgcgct ggcccgcgag gcgtgggcgc cggcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat