BuceA.18439.a

Putative uncharacterized protein

CENTER ID: BuceA.18439.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18439.a.B1.GE35017 full length 1 307
BuceA.18439.a.B2.GE42111 PF08340_0 DUF 224 307
BuceA.18439.a.B3.GE42112 PF03755_0 DUF 2 159
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18439.a.B1.PW36253 full length 1 307

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3126
RefSeq: YP_002232123.1
UniProt: B4EBP7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIYSMTGYAS ATRELVTATG NGGTSVSVEL RTVNSRFLDL NFRMPDDVRA CEPALREMLM NKLSRGKVDV RINLQRGEQS IGAGALNQSA LGQLAELERA VLDSFPGVGR LRAGEILRWP GVLAESGVSA EAIRDAVLAC GKEAIGELVV VRSREGAQLA TMLLSNVTEM EAIVARITPL VPELIAKHQQ KIVERLQEAL GLAAPEGGST VVTREEAAER IRQEVTMYGI RIDIAEELSR LTAHLNETRH VIEKGGRVGK RLDFMMQELN REANTLGSKA AAKELADASM ALKLLIEQMR EQVQNLE
NT Sequence
atgatctaca gcatgacggg ctacgcgagc gcgacgcgcg aactcgtgac ggccaccggc aacggcggca ccagcgtgtc ggtcgaactg cgcaccgtga actcgcgctt cctcgatctc aatttccgga tgccggacga cgtgcgcgcg tgcgaacccg cgctgcgcga aatgctgatg aacaagctgt cgcgcggcaa ggtcgacgtg cgcatcaacc tgcagcgcgg cgagcagagc atcggcgcgg gcgcgctgaa ccagtcggcg ctcggccagc ttgccgagct cgagcgcgcg gtgctcgatt cgttcccggg cgtcggccgc ctgcgcgcgg gcgaaatcct gcgctggccc ggcgtgctcg ccgagagcgg cgtgtccgcg gaagcgatcc gcgacgcggt gctcgcctgc ggcaaggaag cgatcggcga gctcgtcgtc gtgcgttcgc gcgaaggcgc gcaactggcg acgatgctgc tgtcgaacgt taccgagatg gaggcgatcg tcgcgcgcat cacgccgctc gtgccggagc tgatcgcgaa gcatcagcag aagatcgtcg agcggctgca ggaagcgctc ggcctcgcgg cgccggaagg cggctcgacg gtcgtcacgc gcgaggaagc cgcggagcgc atccgtcagg aagtgacgat gtacgggatc cggatcgaca tcgcggaaga gctgtcgcgg ctcaccgcgc acctgaacga aacgcgtcac gtgatcgaga agggcggccg cgtcggcaag cgtctcgact tcatgatgca ggaactgaat cgcgaagcga acacgctcgg ctcgaaggcg gcggcgaagg aactcgccga tgcgtcgatg gcgctgaagc tgctgatcga gcagatgcgc gagcaagtgc aaaacctgga gtaa
Details for BuceA.18439.a.B1.GE35017
HARVESTED ON: 3/19/2012
SEQUENCED ON: 3/22/2012
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMI YSMTGYASAT RELVTATGNG GTSVSVELRT VNSRFLDLNF RMPDDVRACE PALREMLMNK LSRGKVDVRI NLQRGEQSIG AGALNQSALG QLAELERAVL DSFPGVGRLR AGEILRWPGV LAESGVSAEA IRDAVLACGK EAIGELVVVR SREGAQLATM LLSNVTEMEA IVARITPLVP ELIAKHQQKI VERLQEALGL AAPEGGSTVV TREEAAERIR QEVTMYGIRI DIAEELSRLT AHLNETRHVI EKGGRVGKRL DFMMQELNRE ANTLGSKAAA KELADASMAL KLLIEQMREQ V
Validated NT Sequence
ttgcacttgc tcgcgcatct gctcgatcag cagcttcagc gccatcgacg catcggcgag ttccttcgcc gccgccttcg agccgagcgt gttcgcttcg cgattcagtt cctgcatcat gaagtcgaga cgcttgccga cgcggccgcc cttctcgatc acgtgacgcg tttcgttcag gtgcgcggtg agccgcgaca gctcttccgc gatgtcgatc cggatcccgt acatcgtcac ttcctgacgg atgcgctccg cggcttcctc gcgcgtgacg accgtcgagc cgccttccgg cgccgcgagg ccgagcgctt cctgcagccg ctcgacgatc ttctgctgat gcttcgcgat cagctccggc acgagcggcg tgatgcgcgc gacgatcgcc tccatctcgg taacgttcga cagcagcatc gtcgccagtt gcgcgccttc gcgcgaacgc acgacgacga gctcgccgat cgcttccttg ccgcaggcga gcaccgcgtc gcggatcgct tccgcggaca cgccgctctc ggcgagcacg ccgggccagc gcaggatttc gcccgcgcgc aggcggccga cgcccgggaa cgaatcgagc accgcgcgct cgagctcggc aagctggccg agcgccgact ggttcagcgc gcccgcgccg atgctctgct cgccgcgctg caggttgatg cgcacgtcga ccttgccgcg cgacagcttg ttcatcagca tttcgcgcag cgcgggttcg cacgcgcgca cgtcgtccgg catccggaaa ttgagatcga ggaagcgcga gttcacggtg cgcagttcga ccgacacgct ggtgccgccg ttgccggtgg ccgtcacgag ttcgcgcgtc gcgctcgcgt agcccgtcat gctgtagatc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMI YSMTGYASAT RELVTATGNG GTSVSVELRT VNSRFLDLNF RMPDDVRACE PALREMLMNK LSRGKVDVRI NLQRGEQSIG AGALNQSALG QLAELERAVL DSFPGVGRLR AGEILRWPGV LAESGVSAEA IRDAVLACGK EAIGELVVVR SREGAQLATM LLSNVTEMEA IVARITPLVP ELIAKHQQKI VERLQEALGL AAPEGGSTVV TREEAAERIR QEVTMYGIRI DIAEELSRLT AHLNETRHVI EKGGRVGKRL DFMMQELNRE ANTLGSKAAA KELADASMAL KLLIEQMREQ VQNLE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatct acagcatgac gggctacgcg agcgcgacgc gcgaactcgt gacggccacc ggcaacggcg gcaccagcgt gtcggtcgaa ctgcgcaccg tgaactcgcg cttcctcgat ctcaatttcc ggatgccgga cgacgtgcgc gcgtgcgaac ccgcgctgcg cgaaatgctg atgaacaagc tgtcgcgcgg caaggtcgac gtgcgcatca acctgcagcg cggcgagcag agcatcggcg cgggcgcgct gaaccagtcg gcgctcggcc agcttgccga gctcgagcgc gcggtgctcg attcgttccc gggcgtcggc cgcctgcgcg cgggcgaaat cctgcgctgg cccggcgtgc tcgccgagag cggcgtgtcc gcggaagcga tccgcgacgc ggtgctcgcc tgcggcaagg aagcgatcgg cgagctcgtc gtcgtgcgtt cgcgcgaagg cgcgcaactg gcgacgatgc tgctgtcgaa cgttaccgag atggaggcga tcgtcgcgcg catcacgccg ctcgtgccgg agctgatcgc gaagcatcag cagaagatcg tcgagcggct gcaggaagcg ctcggcctcg cggcgccgga aggcggctcg acggtcgtca cgcgcgagga agccgcggag cgcatccgtc aggaagtgac gatgtacggg atccggatcg acatcgcgga agagctgtcg cggctcaccg cgcacctgaa cgaaacgcgt cacgtgatcg agaagggcgg ccgcgtcggc aagcgtctcg acttcatgat gcaggaactg aatcgcgaag cgaacacgct cggctcgaag gcggcggcga aggaactcgc cgatgcgtcg atggcgctga agctgctgat cgagcagatg cgcgagcaag tgcaaaacct ggagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuceA.18439.a.B2.GE42111
HARVESTED ON: 1/16/2018
SEQUENCED ON: 1/17/2018
EXPECTED MW: 11kDa
OBSERVED MW: 11kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHME VTMYGIRIDI AEELSRLTAH LNETRHVIEK GGRVGKRLDF MMQELNREAN TLGSKAAAKE LADASMALKL LIEQMREQVQ NLE
Validated NT Sequence
atggctcacc accaccacca ccatatggaa gtgacgatgt acgggatccg gatcgacatc gcggaagagc tgtcgcggct caccgcgcac ctgaacgaaa cgcgtcacgt gatcgagaag ggcggccgcg tcggcaagcg tctcgacttc atgatgcagg aactgaatcg cgaagcgaac acgctcggct cgaaggcggc ggcgaaggaa ctcgccgatg cgtcgatggc gctgaagctg ctgatcgagc agatgcgcga gcaagtgcaa aacctggagt aagtgagtaa gat
Expected Protein Sequence
MAHHHHHHEV TMYGIRIDIA EELSRLTAHL NETRHVIEKG GRVGKRLDFM MQELNREANT LGSKAAAKEL ADASMALKLL IEQMREQVQN LE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaagtga cgatgtacgg gatccggatc gacatcgcgg aagagctgtc gcggctcacc gcgcacctga acgaaacgcg tcacgtgatc gagaagggcg gccgcgtcgg caagcgtctc gacttcatga tgcaggaact gaatcgcgaa gcgaacacgc tcggctcgaa ggcggcggcg aaggaactcg ccgatgcgtc gatggcgctg aagctgctga tcgagcagat gcgcgagcaa gtgcaaaacc tggagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BuceA.18439.a.B3.GE42112
HARVESTED ON: 1/16/2018
SEQUENCED ON: 1/17/2018
EXPECTED MW: 18kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI YSMTGYASAT RELVTATGNG GTSVSVELRT VNSRFLDLNF RMPDDVRACE PALREMLMNK LSRGKVDVRI NLQRGEQSIG AGALNQSALG QLAELERAVL DSFPGVGRLR AGEILRWPGV LAESGVSAEA IRDAVLACGK EAIGELVVVR SREGAQL
Validated NT Sequence
atggctcacc accaccacca ccatatgatc tacagcatga cgggctacgc gagcgcgacg cgcgaactcg tgacggccac cggcaacggc ggcaccagcg tgtcggtcga actgcgcacc gtgaactcgc gcttcctcga tctcaatttc cggatgccgg acgacgtgcg cgcgtgcgaa cccgcgctgc gcgaaatgct gatgaacaag ctgtcgcgcg gcaaggtcga cgtgcgcatc aacctgcagc gcggcgagca gagcatcggc gcgggcgcgc tgaaccagtc ggcgctcggc cagcttgccg agctcgagcg cgcggtgctc gattcgttcc cgggcgtcgg ccgcctgcgc gcgggcgaaa tcctgcgctg gcccggcgtg ctcgccgaga gcggcgtgtc cgcggaagcg atccgcgacg cggtgctcgc ctgcggcaag gaagcgatcg gcgagctcgt cgtcgtgcgt tcgcgcgaag gcgcgcaact gtaagtgagt aagat
Expected Protein Sequence
MAHHHHHHIY SMTGYASATR ELVTATGNGG TSVSVELRTV NSRFLDLNFR MPDDVRACEP ALREMLMNKL SRGKVDVRIN LQRGEQSIGA GALNQSALGQ LAELERAVLD SFPGVGRLRA GEILRWPGVL AESGVSAEAI RDAVLACGKE AIGELVVVRS REGAQL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatctaca gcatgacggg ctacgcgagc gcgacgcgcg aactcgtgac ggccaccggc aacggcggca ccagcgtgtc ggtcgaactg cgcaccgtga actcgcgctt cctcgatctc aatttccgga tgccggacga cgtgcgcgcg tgcgaacccg cgctgcgcga aatgctgatg aacaagctgt cgcgcggcaa ggtcgacgtg cgcatcaacc tgcagcgcgg cgagcagagc atcggcgcgg gcgcgctgaa ccagtcggcg ctcggccagc ttgccgagct cgagcgcgcg gtgctcgatt cgttcccggg cgtcggccgc ctgcgcgcgg gcgaaatcct gcgctggccc ggcgtgctcg ccgagagcgg cgtgtccgcg gaagcgatcc gcgacgcggt gctcgcctgc ggcaaggaag cgatcggcga gctcgtcgtc gtgcgttcgc gcgaaggcgc gcaactgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BuceA.18439.a.B1.PW36253
PURIFICATION DATe: 5/11/2012
CONCENTRATION: 54.99mg/ml
OBSERVED MW: 32kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI YSMTGYASAT RELVTATGNG GTSVSVELRT VNSRFLDLNF RMPDDVRACE PALREMLMNK LSRGKVDVRI NLQRGEQSIG AGALNQSALG QLAELERAVL DSFPGVGRLR AGEILRWPGV LAESGVSAEA IRDAVLACGK EAIGELVVVR SREGAQLATM LLSNVTEMEA IVARITPLVP ELIAKHQQKI VERLQEALGL AAPEGGSTVV TREEAAERIR QEVTMYGIRI DIAEELSRLT AHLNETRHVI EKGGRVGKRL DFMMQELNRE ANTLGSKAAA KELADASMAL KLLIEQMREQ V
Validated NT Sequence
ttgcacttgc tcgcgcatct gctcgatcag cagcttcagc gccatcgacg catcggcgag ttccttcgcc gccgccttcg agccgagcgt gttcgcttcg cgattcagtt cctgcatcat gaagtcgaga cgcttgccga cgcggccgcc cttctcgatc acgtgacgcg tttcgttcag gtgcgcggtg agccgcgaca gctcttccgc gatgtcgatc cggatcccgt acatcgtcac ttcctgacgg atgcgctccg cggcttcctc gcgcgtgacg accgtcgagc cgccttccgg cgccgcgagg ccgagcgctt cctgcagccg ctcgacgatc ttctgctgat gcttcgcgat cagctccggc acgagcggcg tgatgcgcgc gacgatcgcc tccatctcgg taacgttcga cagcagcatc gtcgccagtt gcgcgccttc gcgcgaacgc acgacgacga gctcgccgat cgcttccttg ccgcaggcga gcaccgcgtc gcggatcgct tccgcggaca cgccgctctc ggcgagcacg ccgggccagc gcaggatttc gcccgcgcgc aggcggccga cgcccgggaa cgaatcgagc accgcgcgct cgagctcggc aagctggccg agcgccgact ggttcagcgc gcccgcgccg atgctctgct cgccgcgctg caggttgatg cgcacgtcga ccttgccgcg cgacagcttg ttcatcagca tttcgcgcag cgcgggttcg cacgcgcgca cgtcgtccgg catccggaaa ttgagatcga ggaagcgcga gttcacggtg cgcagttcga ccgacacgct ggtgccgccg ttgccggtgg ccgtcacgag ttcgcgcgtc gcgctcgcgt agcccgtcat gctgtagatc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMI YSMTGYASAT RELVTATGNG GTSVSVELRT VNSRFLDLNF RMPDDVRACE PALREMLMNK LSRGKVDVRI NLQRGEQSIG AGALNQSALG QLAELERAVL DSFPGVGRLR AGEILRWPGV LAESGVSAEA IRDAVLACGK EAIGELVVVR SREGAQLATM LLSNVTEMEA IVARITPLVP ELIAKHQQKI VERLQEALGL AAPEGGSTVV TREEAAERIR QEVTMYGIRI DIAEELSRLT AHLNETRHVI EKGGRVGKRL DFMMQELNRE ANTLGSKAAA KELADASMAL KLLIEQMREQ VQNLE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCT ACAGCATGAC GGGCTACGCG AGCGCGACGC GCGAACTCGT GACGGCCACC GGCAACGGCG GCACCAGCGT GTCGGTCGAA CTGCGCACCG TGAACTCGCG CTTCCTCGAT CTCAATTTCC GGATGCCGGA CGACGTGCGC GCGTGCGAAC CCGCGCTGCG CGAAATGCTG ATGAACAAGC TGTCGCGCGG CAAGGTCGAC GTGCGCATCA ACCTGCAGCG CGGCGAGCAG AGCATCGGCG CGGGCGCGCT GAACCAGTCG GCGCTCGGCC AGCTTGCCGA GCTCGAGCGC GCGGTGCTCG ATTCGTTCCC GGGCGTCGGC CGCCTGCGCG CGGGCGAAAT CCTGCGCTGG CCCGGCGTGC TCGCCGAGAG CGGCGTGTCC GCGGAAGCGA TCCGCGACGC GGTGCTCGCC TGCGGCAAGG AAGCGATCGG CGAGCTCGTC GTCGTGCGTT CGCGCGAAGG CGCGCAACTG GCGACGATGC TGCTGTCGAA CGTTACCGAG ATGGAGGCGA TCGTCGCGCG CATCACGCCG CTCGTGCCGG AGCTGATCGC GAAGCATCAG CAGAAGATCG TCGAGCGGCT GCAGGAAGCG CTCGGCCTCG CGGCGCCGGA AGGCGGCTCG ACGGTCGTCA CGCGCGAGGA AGCCGCGGAG CGCATCCGTC AGGAAGTGAC GATGTACGGG ATCCGGATCG ACATCGCGGA AGAGCTGTCG CGGCTCACCG CGCACCTGAA CGAAACGCGT CACGTGATCG AGAAGGGCGG CCGCGTCGGC AAGCGTCTCG ACTTCATGAT GCAGGAACTG AATCGCGAAG CGAACACGCT CGGCTCGAAG GCGGCGGCGA AGGAACTCGC CGATGCGTCG ATGGCGCTGA AGCTGCTGAT CGAGCAGATG CGCGAGCAAG TGCAAAACCT GGAGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT