SSGCID: Target Page

BuceA.18433.a

Bifunctional purine biosynthesis protein purH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) (AICAR transformylase); IMP cyclohydrolase (EC 3.5.4.10) (ATIC) (IMP synthase) (Inosinicase)]

CENTER ID:	BuceA.18433.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18433.a.B1.GE35040	full length		1	521

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18433.a.B1.PW35655	full length		1	521

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.3459
RefSeq:	YP_002232439.1
UniProt:	B4EES4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIKQALISVS DKTGIVDFAK SLSDLGVKLL STGGTAKLLA DAGLPVTEVA DYTGFPEMLD GRVKTLHPKV HGGILARRDL PEHMQALEQH GIPTIDLLVV NLYPFVATIA KDDCTLADAI ENIDIGGPTM LRSAAKNHRD VTVVVDPADY AVVLDEMKAN GNTVGYPTNF RLATKVFAHT AQYDGAITNY LTSLTDELKH ASRSAYPATL NLAFDKVQDL RYGENPHQSA AFYRDLAAPA GALANYRQLQ GKELSYNNIA DSDAAWECVK TFDAPACVII KHANPCGVAV GNDSADAYAK AFQTDPTSAF GGIIAFNREV DEAAAQAVAK QFVEVLIAPS FSDAAKQVFA AKQNVRLLEI ALGDGHNAFD LKRVGGGLLV QSLDSKNVQP HELRVVTKRH PTPKEMDDLL FAWRVAKYVK SNAIVFCGNG MTLGVGAGQM SRVDSARIAS IKAQNAGLTL TGSAVASDAF FPFRDGLDVV VAAGATCVIQ PGGSMRDDEV IAAADEHNIA MILTGVRHFR H

NT Sequence

atgatcaagc aagcgctcat ttccgtttcc gacaagaccg gcatcgtcga cttcgcgaag tcgctgtccg acctcggcgt caagctgctg tcgacgggcg gcaccgcgaa actcctcgcc gacgcgggcc tgcccgtgac cgaagtggcc gattacacgg gcttcccgga aatgctcgat gggcgcgtga agacgctcca cccgaaggtg cacggcggca tcctcgcgcg ccgcgacctg cccgagcaca tgcaggcgct cgaacagcac ggcatcccga cgatcgacct gctcgtcgtg aacctgtatc cgttcgtcgc gacgatcgcg aaggacgact gcacgctcgc cgacgcgatc gagaacatcg acatcggcgg cccgacgatg ctgcgctcgg ccgcgaagaa ccaccgcgac gtgacggtcg tcgtcgatcc ggccgactac gcggtcgtgc tcgatgaaat gaaggcgaac ggcaatacgg tcggttaccc gaccaacttc cgcctcgcga cgaaggtgtt cgcgcacacc gcgcaatacg acggcgcgat cacgaactac ctgacgagcc tgaccgacga gctgaagcac gcatcgcgca gcgcgtaccc ggccacgctg aacctggcgt tcgacaaggt tcaggacctg cgctacggcg agaacccgca ccagagcgcg gcgttctacc gcgacctcgc ggcgccggcc ggcgcgctgg cgaactaccg ccagttgcag ggcaaggaac tgtcgtacaa caacatcgcg gattcggacg cggcgtggga atgcgtgaag acgttcgacg cgccggcctg cgtgatcatc aagcatgcga acccgtgcgg cgtcgcggtc ggcaacgact cggccgacgc gtacgcgaag gccttccaga ccgacccgac ctcggcattc ggcggcatca tcgcgttcaa ccgcgaagtc gacgaagcgg ccgcgcaagc cgttgcgaag cagttcgtcg aagtgctgat cgccccgtcg ttctccgacg ccgcgaagca ggtgttcgcc gcgaagcaga acgtgcgcct gctcgaaatc gcgctgggcg acggccataa cgcattcgac ctgaagcgcg tcggcggcgg cctgctcgtg cagtcgctcg attcgaagaa cgtgcagccg cacgagctgc gcgtcgtcac gaagcggcac ccgacgccga aggaaatgga cgacctgctg ttcgcatggc gcgtcgcgaa gtacgtgaag tcgaacgcga tcgtgttctg cggcaatggc atgacgctcg gcgtcggcgc cggccagatg agccgcgtcg attccgcccg catcgcgagc atcaaggcgc agaacgccgg cctgacgctg accggctcgg ccgtcgcgtc ggatgcgttc ttcccgttcc gcgacggcct cgacgtcgtc gtggcggccg gcgcgacctg cgtgatccag ccgggcggct cgatgcgcga cgacgaagtg atcgcggcag ccgacgagca caacatcgcg atgatcctga cgggcgtgcg ccacttccgt cactga

Details for BuceA.18433.a.B1.GE35040

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	85

Validated AA Sequence

MLDGRVKTLH PKVHGGILAR RDLPEHMQAL EQHGIPTIDL LVVNLYPFVA TIAKDDCTLA DAIENIDIGG PTMLRSAAKN HRDVTVVVDP ADYAVVLDEM KANGNTVGYP TNFRLATXVF AHTAQYDGAI TNYLTSLTDE LKHASRSAYP ATLNLAFDKV QDLRYGENPH QSAAFYRDLA APAGALANYR QLQGKELSYN NIADSDAAWE CVKTFDAPXC VIIKHANPCG VAVGNDSADA YAKAFQTDPT SAFGGIIAFN REVDEAAAQA VAKQFVEVLI APSFSDAAKQ VFAAKQNVRL LEIALGDGHN AFDLKRVGGG LLVQSLDSKN VQPHELRVVT KRHPTPKEMD DLLFAWRVAK YVKSNAIVFC GNGMTLGVGA GQMSRVDSAR IASIKAQNAG LTLTGSAVAS DAFFPFRDGL DVVVAAGATC VIQPGGSMRD DEVIAAADEH NIAM

Validated NT Sequence

atcatcgcga tgttgtgctc gtcggctgcc gcgatcactt cgtcgtcgcg catcgagccg cccggctgga tcacgcaggt cgcgccggcc gccacgacga cgtcgaggcc gtcgcggaac gggaagaacg catccgacgc gacggccgag ccggtcagcg tcaggccggc gttctgcgcc ttgatgctcg cgatgcgggc ggaatcgacg cggctcatct ggccggcgcc gacgccgagc gtcatgccat tgccgcagaa cacgatcgcg ttcgacttca cgtacttcgc gacgcgccat gcgaacagca ggtcgtccat ttccttcggc gtcgggtgcc gcttcgtgac gacgcgcagc tcgtgcggct gcacgttctt cgaatcgagc gactgcacga gcaggccgcc gccgacgcgc ttcaggtcga atgcgttatg gccgtcgccc agcgcgattt cgagcaggcg cacgttctgc ttcgcggcga acacctgctt cgcggcgtcg gagaacgacg gggcgatcag cacttcgacg aactgcttcg caacggcttg cgcggccgct tcgtcgactt cgcggttgaa cgcgatgatg ccgccgaatg ccgaggtcgg gtcggtctgg aaggccttcg cgtacgcgtc ggccgagtcg ttgccgaccg cgacgccgca cgggttcgca tgcttgatga tcacgcagnc nggcgcgtcg aacgtcttca cgcattccca cgccgcgtcc gaatccgcga tgttgttgta cgacagttcc ttgccctgca actggcggta gttcgccagc gcgccggccg gcgccgcgag gtcgcggtag aacgccgcgc tctggtgcgg gttctcgccg tagcgcaggt cctgaacctt gtcgaacgcc aggttcagcg tggccgggta cgcgctgcgc gatgcgtgct tcagctcgtc ggtcaggctc gtcaggtagt tcgtgatcgc gccgtcgtat tgcgcggtgt gcgcgaacac nttcgtcgcg aggcggaagt tggtcgggta accgaccgta ttgccgttcg ccttcatttc atcgagcacg accgcgtagt cggccggatc gacgacgacc gtcacgtcgc ggtggttctt cgcggccgag cgcagcatcg tcgggccgcc gatgtcgatg ttctcgatcg cgtcggcgag cgtgcagtcg tccttcgcga tcgtcgcgac gaacggatac aggttcacga cgagcaggtc gatcgtcggg atgccgtgct gttcgagcgc ctgcatgtgc tcgggcaggt cgcggcgcgc gaggatgccg ccgtgcacct tcgggtggag cgtcttcacg cgcccatcga gcatttccgg gaagcccgtg taatcggcca cttcggtcac gggcaggccc gcgtcggcga ggagtttcgc ggtgccgccc gtcgacagca gcttgacgcc gaggtcggac agcgacttcg cgaagtcgac gatgccggtc ttgtcggaaa cggaaatgag cgcttg

Expected Protein Sequence

MAHHHHHHMI KQALISVSDK TGIVDFAKSL SDLGVKLLST GGTAKLLADA GLPVTEVADY TGFPEMLDGR VKTLHPKVHG GILARRDLPE HMQALEQHGI PTIDLLVVNL YPFVATIAKD DCTLADAIEN IDIGGPTMLR SAAKNHRDVT VVVDPADYAV VLDEMKANGN TVGYPTNFRL ATKVFAHTAQ YDGAITNYLT SLTDELKHAS RSAYPATLNL AFDKVQDLRY GENPHQSAAF YRDLAAPAGA LANYRQLQGK ELSYNNIADS DAAWECVKTF DAPACVIIKH ANPCGVAVGN DSADAYAKAF QTDPTSAFGG IIAFNREVDE AAAQAVAKQF VEVLIAPSFS DAAKQVFAAK QNVRLLEIAL GDGHNAFDLK RVGGGLLVQS LDSKNVQPHE LRVVTKRHPT PKEMDDLLFA WRVAKYVKSN AIVFCGNGMT LGVGAGQMSR VDSARIASIK AQNAGLTLTG SAVASDAFFP FRDGLDVVVA AGATCVIQPG GSMRDDEVIA AADEHNIAMI LTGVRHFRH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatca agcaagcgct catttccgtt tccgacaaga ccggcatcgt cgacttcgcg aagtcgctgt ccgacctcgg cgtcaagctg ctgtcgacgg gcggcaccgc gaaactcctc gccgacgcgg gcctgcccgt gaccgaagtg gccgattaca cgggcttccc ggaaatgctc gatgggcgcg tgaagacgct ccacccgaag gtgcacggcg gcatcctcgc gcgccgcgac ctgcccgagc acatgcaggc gctcgaacag cacggcatcc cgacgatcga cctgctcgtc gtgaacctgt atccgttcgt cgcgacgatc gcgaaggacg actgcacgct cgccgacgcg atcgagaaca tcgacatcgg cggcccgacg atgctgcgct cggccgcgaa gaaccaccgc gacgtgacgg tcgtcgtcga tccggccgac tacgcggtcg tgctcgatga aatgaaggcg aacggcaata cggtcggtta cccgaccaac ttccgcctcg cgacgaaggt gttcgcgcac accgcgcaat acgacggcgc gatcacgaac tacctgacga gcctgaccga cgagctgaag cacgcatcgc gcagcgcgta cccggccacg ctgaacctgg cgttcgacaa ggttcaggac ctgcgctacg gcgagaaccc gcaccagagc gcggcgttct accgcgacct cgcggcgccg gccggcgcgc tggcgaacta ccgccagttg cagggcaagg aactgtcgta caacaacatc gcggattcgg acgcggcgtg ggaatgcgtg aagacgttcg acgcgccggc ctgcgtgatc atcaagcatg cgaacccgtg cggcgtcgcg gtcggcaacg actcggccga cgcgtacgcg aaggccttcc agaccgaccc gacctcggca ttcggcggca tcatcgcgtt caaccgcgaa gtcgacgaag cggccgcgca agccgttgcg aagcagttcg tcgaagtgct gatcgccccg tcgttctccg acgccgcgaa gcaggtgttc gccgcgaagc agaacgtgcg cctgctcgaa atcgcgctgg gcgacggcca taacgcattc gacctgaagc gcgtcggcgg cggcctgctc gtgcagtcgc tcgattcgaa gaacgtgcag ccgcacgagc tgcgcgtcgt cacgaagcgg cacccgacgc cgaaggaaat ggacgacctg ctgttcgcat ggcgcgtcgc gaagtacgtg aagtcgaacg cgatcgtgtt ctgcggcaat ggcatgacgc tcggcgtcgg cgccggccag atgagccgcg tcgattccgc ccgcatcgcg agcatcaagg cgcagaacgc cggcctgacg ctgaccggct cggccgtcgc gtcggatgcg ttcttcccgt tccgcgacgg cctcgacgtc gtcgtggcgg ccggcgcgac ctgcgtgatc cagccgggcg gctcgatgcg cgacgacgaa gtgatcgcgg cagccgacga gcacaacatc gcgatgatcc tgacgggcgt gcgccacttc cgtcactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for BuceA.18433.a.B1.PW35655

PURIFICATION DATe:	4/20/2012
CONCENTRATION:	32.18mg/ml
OBSERVED MW:	56kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	12
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	85

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCA AGCAAGCGCT CATTTCCGTT TCCGACAAGA CCGGCATCGT CGACTTCGCG AAGTCGCTGT CCGACCTCGG CGTCAAGCTG CTGTCGACGG GCGGCACCGC GAAACTCCTC GCCGACGCGG GCCTGCCCGT GACCGAAGTG GCCGATTACA CGGGCTTCCC GGAAATGCTC GATGGGCGCG TGAAGACGCT CCACCCGAAG GTGCACGGCG GCATCCTCGC GCGCCGCGAC CTGCCCGAGC ACATGCAGGC GCTCGAACAG CACGGCATCC CGACGATCGA CCTGCTCGTC GTGAACCTGT ATCCGTTCGT CGCGACGATC GCGAAGGACG ACTGCACGCT CGCCGACGCG ATCGAGAACA TCGACATCGG CGGCCCGACG ATGCTGCGCT CGGCCGCGAA GAACCACCGC GACGTGACGG TCGTCGTCGA TCCGGCCGAC TACGCGGTCG TGCTCGATGA AATGAAGGCG AACGGCAATA CGGTCGGTTA CCCGACCAAC TTCCGCCTCG CGACGAAGGT GTTCGCGCAC ACCGCGCAAT ACGACGGCGC GATCACGAAC TACCTGACGA GCCTGACCGA CGAGCTGAAG CACGCATCGC GCAGCGCGTA CCCGGCCACG CTGAACCTGG CGTTCGACAA GGTTCAGGAC CTGCGCTACG GCGAGAACCC GCACCAGAGC GCGGCGTTCT ACCGCGACCT CGCGGCGCCG GCCGGCGCGC TGGCGAACTA CCGCCAGTTG CAGGGCAAGG AACTGTCGTA CAACAACATC GCGGATTCGG ACGCGGCGTG GGAATGCGTG AAGACGTTCG ACGCGCCGGC CTGCGTGATC ATCAAGCATG CGAACCCGTG CGGCGTCGCG GTCGGCAACG ACTCGGCCGA CGCGTACGCG AAGGCCTTCC AGACCGACCC GACCTCGGCA TTCGGCGGCA TCATCGCGTT CAACCGCGAA GTCGACGAAG CGGCCGCGCA AGCCGTTGCG AAGCAGTTCG TCGAAGTGCT GATCGCCCCG TCGTTCTCCG ACGCCGCGAA GCAGGTGTTC GCCGCGAAGC AGAACGTGCG CCTGCTCGAA ATCGCGCTGG GCGACGGCCA TAACGCATTC GACCTGAAGC GCGTCGGCGG CGGCCTGCTC GTGCAGTCGC TCGATTCGAA GAACGTGCAG CCGCACGAGC TGCGCGTCGT CACGAAGCGG CACCCGACGC CGAAGGAAAT GGACGACCTG CTGTTCGCAT GGCGCGTCGC GAAGTACGTG AAGTCGAACG CGATCGTGTT CTGCGGCAAT GGCATGACGC TCGGCGTCGG CGCCGGCCAG ATGAGCCGCG TCGATTCCGC CCGCATCGCG AGCATCAAGG CGCAGAACGC CGGCCTGACG CTGACCGGCT CGGCCGTCGC GTCGGATGCG TTCTTCCCGT TCCGCGACGG CCTCGACGTC GTCGTGGCGG CCGGCGCGAC CTGCGTGATC CAGCCGGGCG GCTCGATGCG CGACGACGAA GTGATCGCGG CAGCCGACGA GCACAACATC GCGATGATCC TGACGGGCGT GCGCCACTTC CGTCACTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T