SSGCID: Target Page

BuceA.18231.a

tRNA pseudouridine synthase B (EC 5.4.99.25) (tRNA pseudouridine(55) synthase) (tRNA pseudouridylate synthase) (tRNA-uridine isomerase)

CENTER ID:	BuceA.18231.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18231.a.B1.GE35018	full length		1	310

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.1567
RefSeq:	YP_002230639.1
UniProt:	B4E7L3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTNAAPQRPR VPRRVLDGVL LLDKPVGLSS NDALIRAKRL LLAKKAGHTG TLDPLASGLL PLCFGEATKF SQDLLDADKT YEATMRLGQR TATGDAEGDV IDTRPVECDR AAVEAALVRF TGDIVQVPPM YSALKRDGKP LYEYARAGQT VEREGRNVTI HALELLACEL PDVTFRVTCS KGTYVRTLAE DIGEALGCGA HLTMLRRTGV GALTLEHAVT LDALSDAAEA ARDAWLQPVD ALLSTFPAIR LDDASAKRFL HGQRLPLSAL EPIDAADGER VRVYDATRLL GVARKANGVL APERLVVTAQ

NT Sequence

atgacgaatg cagcacccca acgtccgcgc gtgccccggc gcgtgctgga cggcgttctc ctgctcgaca agccggtcgg cctgtcgagc aacgatgcgt tgattcgcgc aaagcgcctg cttctcgcga agaaagccgg tcacaccggc acgctcgatc cgctggcttc gggcctgctg ccgctgtgct tcggcgaggc gacgaagttc tcgcaggact tgctcgacgc cgacaagacc tatgaagcga cgatgcgcct cggccagcgc acggctaccg gtgacgcgga aggcgacgtg atcgacacgc ggccggtcga atgcgatcgt gcggcggtgg aagccgcgct cgtgcgcttc accggcgaca tcgtgcaggt gccgccgatg tattcggcgc tcaagcgcga cggcaagccg ctgtatgaat atgcgcgtgc gggccagacc gtcgagcggg agggccgcaa cgtgacgatt catgcgctcg aactgctcgc ctgcgaactg cccgacgtga cgtttcgcgt gacctgcagc aagggcacgt atgtgcgcac gctggcggag gatatcggcg aggcgctggg ttgcggcgcg catctgacga tgctgcgtcg cacgggcgtc ggcgcgctga cgctcgagca tgcggtgacg ctcgatgcgc tgtcggacgc tgccgaagcg gcgcgcgatg catggctgca gccggtcgat gcgttgctgt cgacgttccc ggccattcgg ctcgacgacg cgtccgcgaa gcggttcctg catggtcagc gcctgccgct gtcggcgctg gagccgatcg acgcggccga tggcgagcgc gtgcgtgtgt atgacgcgac gcggttgctc ggcgtggcgc gcaaggcgaa tggcgtgctc gccccggaac ggctggtcgt gacggcgcaa tga

Details for BuceA.18231.a.B1.GE35018

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

cacttattgc gccgtcacga ccagccgttc cggggcgagc acgccattcg ccttgcgcgc cacgccgagc aaccgcgtcg cgtcatacac acgcacgcgc tcgccatcgg ccgcgtcgat cggctccagc gccgacagcg gcaggcgctg accatgcagg aaccgcttcg cggacgcgtc gtcgagccga atggccggga acgtcgacag caacgcatcg accggctgca gccatgcatc gcgcgccgct tcggcagcgt ccgacagcgc atcgagcgtc accgcatgct cgagcgtcag cgcgccgacg cccgtgcgac gcagcatcgt cagatgcgcg ccgcaaccca gcgcctcgcc gatatcctcc gccagcgtgc gcacatacgt gcccttgctg caggtcacgc gaaacgtcac gtcgggcagt tcgcaggcga gcagttcgag cgcatgaatc gtcacgttgc ggccctcccg ctcgacggtc tggcccgcac gcgcatattc atacagcggc ttgccgtcgc gcttgagcgc cgaatacatc ggcggcacct gcacgatgtc gccggtgaag cgcacgagcg cggcttccac cgccgcacga tcgcattcga ccggccgcgt gtcgatcacg tcgccttccg cgtcaccggt agccgtgcgc tggccgaggc gcatcgtcgc ttcataggtc ttgtcggcgt cgagcaagtc ctgcgagaac ttcgtcgcct cgccgaagca cagcggcagc aggcccgaag ccagcggatc gagcgtgccg gtgtgaccgg ctttcttcgc gagaagcagg cgctttgcgc gaatcaacgc atcgttgctc gacaggccga ccggcttgtc gagcaggaga acgccgtcca gcacgcgccg gggcacgcgc ggacgttggg gtgctgcatt cgtcatatgg tggtggtgg

Expected Protein Sequence

MAHHHHHHMT NAAPQRPRVP RRVLDGVLLL DKPVGLSSND ALIRAKRLLL AKKAGHTGTL DPLASGLLPL CFGEATKFSQ DLLDADKTYE ATMRLGQRTA TGDAEGDVID TRPVECDRAA VEAALVRFTG DIVQVPPMYS ALKRDGKPLY EYARAGQTVE REGRNVTIHA LELLACELPD VTFRVTCSKG TYVRTLAEDI GEALGCGAHL TMLRRTGVGA LTLEHAVTLD ALSDAAEAAR DAWLQPVDAL LSTFPAIRLD DASAKRFLHG QRLPLSALEP IDAADGERVR VYDATRLLGV ARKANGVLAP ERLVVTAQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga atgcagcacc ccaacgtccg cgcgtgcccc ggcgcgtgct ggacggcgtt ctcctgctcg acaagccggt cggcctgtcg agcaacgatg cgttgattcg cgcaaagcgc ctgcttctcg cgaagaaagc cggtcacacc ggcacgctcg atccgctggc ttcgggcctg ctgccgctgt gcttcggcga ggcgacgaag ttctcgcagg acttgctcga cgccgacaag acctatgaag cgacgatgcg cctcggccag cgcacggcta ccggtgacgc ggaaggcgac gtgatcgaca cgcggccggt cgaatgcgat cgtgcggcgg tggaagccgc gctcgtgcgc ttcaccggcg acatcgtgca ggtgccgccg atgtattcgg cgctcaagcg cgacggcaag ccgctgtatg aatatgcgcg tgcgggccag accgtcgagc gggagggccg caacgtgacg attcatgcgc tcgaactgct cgcctgcgaa ctgcccgacg tgacgtttcg cgtgacctgc agcaagggca cgtatgtgcg cacgctggcg gaggatatcg gcgaggcgct gggttgcggc gcgcatctga cgatgctgcg tcgcacgggc gtcggcgcgc tgacgctcga gcatgcggtg acgctcgatg cgctgtcgga cgctgccgaa gcggcgcgcg atgcatggct gcagccggtc gatgcgttgc tgtcgacgtt cccggccatt cggctcgacg acgcgtccgc gaagcggttc ctgcatggtc agcgcctgcc gctgtcggcg ctggagccga tcgacgcggc cgatggcgag cgcgtgcgtg tgtatgacgc gacgcggttg ctcggcgtgg cgcgcaaggc gaatggcgtg ctcgccccgg aacggctggt cgtgacggcg caatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat