BuceA.18086.a

LuxR superfamily regulatory protein

CENTER ID: BuceA.18086.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18086.a.A1.GE33255 full length 1 265
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18086.a.A1.PW33865 full length 1 265

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1432
RefSeq: YP_002230510.1
UniProt: B4E6A1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLTLQDVAW HRSVGRLIET LDQPGFWLSL IRLIEEYVAV DSWVALMFGD GRPQVFAECA YEGGGPDPLF RDYVQGLYLL DPFYIANRDA PTSGLFRLSD VAPECFRDTE YYTRYFTHNV VEDEVQFNVV LDDTRTLCLS LGSKRRFSPE HVALLDLVRP WVAALMRQRF AYEPMLAEPA PSRRPGADAG NGFEHAMARL GTPLTARELD VIRLILSGRS NKEVASKLDI SAETVKVHRR NIYAKLAINS QSELFALFLK AQTDT
NT Sequence
atgaacctga ccttgcagga cgtcgcctgg caccgctcgg tcgggcggct catcgagacg ctcgaccagc cgggtttctg gctgtcgctg atccggctga tcgaggaata cgtcgcggtc gacagctggg tcgcgctgat gttcggcgac ggccgtccgc aggtgttcgc cgaatgcgcg tacgaaggcg gcggccccga cccgctgttc cgcgactacg tgcaggggct gtacctgctg gacccgttct acatcgcgaa ccgcgacgcg ccgacgagcg gcctgttccg gctgtccgac gtcgcgcccg agtgctttcg cgacaccgag tactacacgc gctatttcac gcacaacgtc gtcgaggacg aggtgcagtt caacgtcgtg ctcgatgaca cgcgcacgct gtgcctgtcg ctcggcagca agcggcgctt cagccccgaa cacgtggcac tgctcgacct ggtgcggccg tgggtcgccg cgctgatgcg ccagcgcttc gcatacgagc ccatgctggc cgaaccggct ccgtcgcgcc ggcccggcgc cgatgccggg aacggcttcg agcacgcgat ggcacggctc ggcacgccgc tcaccgcgcg cgagctcgac gtgatccggc tgatcctgag cggacgctcg aacaaggaag tcgcgagcaa gctggacatc tcggccgaaa cggtcaaggt gcaccggcgc aacatctacg cgaagctcgc gatcaattcg cagtctgaac tgtttgcgct gttcctgaag gcgcagaccg atacgtga
Details for BuceA.18086.a.A1.GE33255
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMNLTLQD VAWHRSVGRL IETLDQPGFW LSLIRLIEEY VAVDSWVALM FGDGRPQVFA ECAYEGGGPD PLFRDYVQGL YLLDPFYIAN RDAPTSGLFR LSDVAPECFR DTEYYTRYFT HNVVEDEVQF NVVLDDTRTL CLSLGSKRRF SPEHVALLDL VRPWVAALMR QRFAYEPMLA EPAPSRRPGA DAGNGFEHAM ARLGTPLTAR ELDVIRLILS GRSNKEVASK LDISAETVKV HRRNIYAKLA INSQSELFAL FLKAQT
Validated NT Sequence
cggtctgcgc cttcaggaac agcgcaaaca gttcagactg cgaattgatc gcgagcttcg cgtagatgtt gcgccggtgc accttgaccg tttcggccga gatgtccagc ttgctcgcga cttccttgtt cgagcgtccg ctcaggatca gccggatcac gtcgagctcg cgcgcggtga gcggcgtgcc gagccgtgcc atcgcgtgct cgaagccgtt cccggcatcg gcgccgggcc ggcgcgacgg agccggttcg gccagcatgg gctcgtatgc gaagcgctgg cgcatcagcg cggcgaccca cggccgcacc aggtcgagca gtgccacgtg ttcggggctg aagcgccgct tgctgccgag cgacaggcac agcgtgcgcg tgtcatcgag cacgacgttg aactgcacct cgtcctcgac gacgttgtgc gtgaaatagc gcgtgtagta ctcggtgtcg cgaaagcact cgggcgcgac gtcggacagc cggaacaggc cgctcgtcgg cgcgtcgcgg ttcgcgatgt agaacgggtc cagcaggtac agcccctgca cgtagtcgcg gaacagcggg tcggggccgc cgccttcgta cgcgcattcg gcgaacacct gcggacggcc gtcgccgaac atcagcgcga cccagctgtc gaccgcgacg tattcctcga tcagccggat cagcgacagc cagaaacccg gctggtcgag cgtctcgatg agccgcccga ccgagcggtg ccaggcgacg tcctgcaagg tcaggttcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLTLQDVA WHRSVGRLIE TLDQPGFWLS LIRLIEEYVA VDSWVALMFG DGRPQVFAEC AYEGGGPDPL FRDYVQGLYL LDPFYIANRD APTSGLFRLS DVAPECFRDT EYYTRYFTHN VVEDEVQFNV VLDDTRTLCL SLGSKRRFSP EHVALLDLVR PWVAALMRQR FAYEPMLAEP APSRRPGADA GNGFEHAMAR LGTPLTAREL DVIRLILSGR SNKEVASKLD ISAETVKVHR RNIYAKLAIN SQSELFALFL KAQTDT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacctgacc ttgcaggacg tcgcctggca ccgctcggtc gggcggctca tcgagacgct cgaccagccg ggtttctggc tgtcgctgat ccggctgatc gaggaatacg tcgcggtcga cagctgggtc gcgctgatgt tcggcgacgg ccgtccgcag gtgttcgccg aatgcgcgta cgaaggcggc ggccccgacc cgctgttccg cgactacgtg caggggctgt acctgctgga cccgttctac atcgcgaacc gcgacgcgcc gacgagcggc ctgttccggc tgtccgacgt cgcgcccgag tgctttcgcg acaccgagta ctacacgcgc tatttcacgc acaacgtcgt cgaggacgag gtgcagttca acgtcgtgct cgatgacacg cgcacgctgt gcctgtcgct cggcagcaag cggcgcttca gccccgaaca cgtggcactg ctcgacctgg tgcggccgtg ggtcgccgcg ctgatgcgcc agcgcttcgc atacgagccc atgctggccg aaccggctcc gtcgcgccgg cccggcgccg atgccgggaa cggcttcgag cacgcgatgg cacggctcgg cacgccgctc accgcgcgcg agctcgacgt gatccggctg atcctgagcg gacgctcgaa caaggaagtc gcgagcaagc tggacatctc ggccgaaacg gtcaaggtgc accggcgcaa catctacgcg aagctcgcga tcaattcgca gtctgaactg tttgcgctgt tcctgaaggc gcagaccgat acgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BuceA.18086.a.A1.PW33865
PURIFICATION DATe: 11/19/2011
CONCENTRATION: 29.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMNLTLQD VAWHRSVGRL IETLDQPGFW LSLIRLIEEY VAVDSWVALM FGDGRPQVFA ECAYEGGGPD PLFRDYVQGL YLLDPFYIAN RDAPTSGLFR LSDVAPECFR DTEYYTRYFT HNVVEDEVQF NVVLDDTRTL CLSLGSKRRF SPEHVALLDL VRPWVAALMR QRFAYEPMLA EPAPSRRPGA DAGNGFEHAM ARLGTPLTAR ELDVIRLILS GRSNKEVASK LDISAETVKV HRRNIYAKLA INSQSELFAL FLKAQT
Validated NT Sequence
cggtctgcgc cttcaggaac agcgcaaaca gttcagactg cgaattgatc gcgagcttcg cgtagatgtt gcgccggtgc accttgaccg tttcggccga gatgtccagc ttgctcgcga cttccttgtt cgagcgtccg ctcaggatca gccggatcac gtcgagctcg cgcgcggtga gcggcgtgcc gagccgtgcc atcgcgtgct cgaagccgtt cccggcatcg gcgccgggcc ggcgcgacgg agccggttcg gccagcatgg gctcgtatgc gaagcgctgg cgcatcagcg cggcgaccca cggccgcacc aggtcgagca gtgccacgtg ttcggggctg aagcgccgct tgctgccgag cgacaggcac agcgtgcgcg tgtcatcgag cacgacgttg aactgcacct cgtcctcgac gacgttgtgc gtgaaatagc gcgtgtagta ctcggtgtcg cgaaagcact cgggcgcgac gtcggacagc cggaacaggc cgctcgtcgg cgcgtcgcgg ttcgcgatgt agaacgggtc cagcaggtac agcccctgca cgtagtcgcg gaacagcggg tcggggccgc cgccttcgta cgcgcattcg gcgaacacct gcggacggcc gtcgccgaac atcagcgcga cccagctgtc gaccgcgacg tattcctcga tcagccggat cagcgacagc cagaaacccg gctggtcgag cgtctcgatg agccgcccga ccgagcggtg ccaggcgacg tcctgcaagg tcaggttcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatgg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLTLQDVA WHRSVGRLIE TLDQPGFWLS LIRLIEEYVA VDSWVALMFG DGRPQVFAEC AYEGGGPDPL FRDYVQGLYL LDPFYIANRD APTSGLFRLS DVAPECFRDT EYYTRYFTHN VVEDEVQFNV VLDDTRTLCL SLGSKRRFSP EHVALLDLVR PWVAALMRQR FAYEPMLAEP APSRRPGADA GNGFEHAMAR LGTPLTAREL DVIRLILSGR SNKEVASKLD ISAETVKVHR RNIYAKLAIN SQSELFALFL KAQTDT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACCTGACC TTGCAGGACG TCGCCTGGCA CCGCTCGGTC GGGCGGCTCA TCGAGACGCT CGACCAGCCG GGTTTCTGGC TGTCGCTGAT CCGGCTGATC GAGGAATACG TCGCGGTCGA CAGCTGGGTC GCGCTGATGT TCGGCGACGG CCGTCCGCAG GTGTTCGCCG AATGCGCGTA CGAAGGCGGC GGCCCCGACC CGCTGTTCCG CGACTACGTG CAGGGGCTGT ACCTGCTGGA CCCGTTCTAC ATCGCGAACC GCGACGCGCC GACGAGCGGC CTGTTCCGGC TGTCCGACGT CGCGCCCGAG TGCTTTCGCG ACACCGAGTA CTACACGCGC TATTTCACGC ACAACGTCGT CGAGGACGAG GTGCAGTTCA ACGTCGTGCT CGATGACACG CGCACGCTGT GCCTGTCGCT CGGCAGCAAG CGGCGCTTCA GCCCCGAACA CGTGGCACTG CTCGACCTGG TGCGGCCGTG GGTCGCCGCG CTGATGCGCC AGCGCTTCGC ATACGAGCCC ATGCTGGCCG AACCGGCTCC GTCGCGCCGG CCCGGCGCCG ATGCCGGGAA CGGCTTCGAG CACGCGATGG CACGGCTCGG CACGCCGCTC ACCGCGCGCG AGCTCGACGT GATCCGGCTG ATCCTGAGCG GACGCTCGAA CAAGGAAGTC GCGAGCAAGC TGGACATCTC GGCCGAAACG GTCAAGGTGC ACCGGCGCAA CATCTACGCG AAGCTCGCGA TCAATTCGCA GTCTGAACTG TTTGCGCTGT TCCTGAAGGC GCAGACCGAT ACGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA