BuceA.18080.a

Putative GMC oxidoreductase

CENTER ID: BuceA.18080.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18080.a.A1.GE33251 full length 1 546
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.542
RefSeq: YP_002229680.1
UniProt: B4E810

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQYDYIIVGG GSGGSSLAGR LADACPDATI ALIEAGSHTE RNLLVNMPVG IAALVPFKLG TNYGYETVPQ PGLGGRRGYQ PRGRGMGGSS AINAMIYTRG HPGDYDEWAQ LGATGWGWQD VLPYFRRAEG NERGADAWHG ADGPLTVSDL RFRNPFSERF IQAAHAAGYP LNDDFNGATQ EGVGFYQVTH RDGSRCSVAR AYIYGRNRPN LHVLTDATVL RVGFDGKRAV GVVVSRNGRV ETLGARAEVI LSAGAFNSPQ LLMCSGIGPA EQLRRHGIAV VQDAPDVGTN LIDHIDFIIN TRVNSSELVG VCVRGIAKMT PALARYFSSR TGMMTSNVAE AGGFIKSDPS LARPDLQLHF CTALVDDHNR KMHWGFGYSL HVCALRPFSR GTVALASGDA RDAPLIDPRF FSDSRDLDLL VRGAQAMRRI LSQAPLASQG GRELYTRPDQ SEAELRATIV AHADTIYHPV GTCRMGSDAR AVVDPQLRVR GVDGLRVVDA SVMPTLVGGN TNAPSVMIGE RAADFIVAAR RGGVPRGEAA TAVHGR
NT Sequence
atgcaatacg actacatcat cgtcggcggc ggttcgggcg gctcgagcct ggccggccgt ctcgccgatg cctgccccga tgcgacgatc gcactgatcg aggccggctc gcatacggag cgcaacctgc tggtcaacat gccggtcggc atcgcggcgc tggtgccgtt caagctcggc acgaactacg gctacgagac ggtgccgcag ccgggcctcg gcgggcggcg cggttaccag ccgcgcgggc gcgggatggg cggctcgagc gcgatcaacg cgatgatcta tacgcgcggc cacccgggcg attacgacga gtgggcgcag ctcggcgcga cgggctgggg ctggcaggac gtgctgccgt atttccgccg cgcggaaggc aacgaacgcg gcgccgacgc gtggcacggc gcggacggcc cgttgaccgt ttccgatctg cgctttcgca atccgttctc cgaacgattc atccaggccg cgcatgccgc cggctatccg ctgaacgacg acttcaacgg cgcgacgcag gaaggcgtcg gcttctatca ggtcacgcat cgcgacggtt cgcgttgcag cgtggcgcgc gcgtatatct acggccgcaa ccggccgaac ctgcacgtgc tcacggatgc gacggtgctg cgcgtcggct tcgacggcaa gcgcgcggtc ggcgtcgtgg tgtcgcgcaa cgggcgggtc gagacgctcg gcgcacgcgc ggaagtgatc ctgtcggccg gcgcgttcaa ttcgccgcaa ctgctgatgt gttcgggaat cggcccggcg gagcaactgc gccgccacgg gatcgcggtc gtgcaggatg cgccggacgt cggcacgaac ctgatcgacc acatcgattt catcatcaac acgcgcgtga attcgtcgga gctggtcggc gtgtgcgtgc gcggcatcgc gaagatgacg ccggcgctcg cgcgctattt ctcgagccgc accgggatga tgacgagcaa cgtcgcggag gccggcggtt tcatcaagag cgatccgtcg ctcgcgcgtc ccgacctgca actgcatttc tgcacggcgc tcgtcgacga ccacaaccgc aagatgcact ggggcttcgg ctattcgctg cacgtgtgcg cgctgcggcc gttcagccgc ggcacggtcg cgctcgcgag cggcgatgcg cgcgacgcgc cgctgatcga tccgcgcttc ttcagcgatt cacgcgatct cgacctgctg gtgcgcggcg cgcaggcgat gcgccgcatc ctgtcgcagg cgccgctcgc gtcgcagggc gggcgcgagc tgtacacgcg gccggaccag agcgaagcgg agctgcgcgc gacgatcgtc gcgcatgcgg acacgatcta tcacccggtc ggcacctgcc ggatgggctc cgatgcgcgc gcggtcgtcg atccgcaact gcgcgtgcgc ggcgtcgacg gattgcgcgt ggtggatgcg tcggtgatgc cgacgctggt gggcgggaac acgaatgcgc cgtcggtgat gatcggcgaa cgcgcggcgg acttcatcgt cgcggcgcgc cgcggcggtg tgccgcgcgg cgaggcggct accgcggtgc acggccgcta g
Details for BuceA.18080.a.A1.GE33251
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 60kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 70
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQYDYIIVG GGSGGSSLAG RLADACPDAT IALIEAGSHT ERNLLVNMPV GIAALVPFKL GTNYGYETVP QPGLGGRRGY QPRGRGMGGS SAINAMIYTR GHPGDYDEWA QLGATGWGWQ DVLPYFRRAE GNERGADAWH GADGPLTVSD LRFRNPFSER FIQAAHAAGY PLNDDFNGAT QEGVGFYQVT HRDGSRCSVA RAYIYGRNRP NLHVLTDATV LRVGFDGKRA VGVVVSRNGR VETLGARAEV ILSAGAFNSP QLLMCSGIGP AXQLRRXGIA VVXXAPDVGT NLIDHIDFII NTRVNSSXLX GVCVRXIXXX TPXLXXXXXX XXXDDXXXXX XXXXSXXXXX XXXTXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
attttgntta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcaat acgactacat catcgtcggc ggcggttcgg gcggctcgag cctggccggc cgtctcgccg atgcctgccc cgatgcgacg atcgcactga tcgaggccgg ctcgcatacg gagcgcaacc tgctggtcaa catgccggtc ggcatcgcgg cgctggtgcc gttcaagctc ggcacgaact acggctacga gacggtgccg cagccgggcc tcggcgggcg gcgcggttac cagccgcgcg ggcgcgggat gggcggctcg agcgcgatca acgcgatgat ctatacgcgc ggccacccgg gcgattacga cgagtgggcg cagctcggcg cgacgggctg gggctggcag gacgtgctgc cgtatttccg ccgcgcggaa ggcaacgaac gcggcgccga cgcgtggcac ggcgcggacg gcccgttgac cgtttccgat ctgcgctttc gcaatccgtt ctccgaacga ttcatccagg ccgcgcatgc cgccggctat ccgctgaacg acgacttcaa cggcgcgacg caggaaggcg tcggcttcta tcaggtcacg catcgcgacg gttcgcgttg cagcgtggcg cgcgcgtata tctacggccg caaccggccg aacctgcacg tgctcacgga tgcgacggtg ctgcgcgtcg gcttcgacgg caagcgcgcg gtcggcgtcg tggtgtcgcg caacgggcgg gtcgagacgc tcggcgcacg cgcggaagtg atcctgtcgg ccggcgcgtt caattcgccg caactgctga tgtgttcngg aatcggcccg gcggancaac tgcgccgccn cgggatcgcg gtcgtgcngn atgcgccgga cgtcggcacg aacctgatcg accacatcga tttcatcatc aacacgcgcg tgaattcgtc gnanctggnc ggcgtgtgcg tgcgnngcat cncgaanang acgccnnngc tcnngcncta nttnntcnan cnncannngg atgatgnnna nnannncnnn nnnngnngnn nntcannnga ncnnnnnntn nnnnntncga cnnnacnnnn tttnnnnnng nnnnannann nnannnnnnn annnnngnnn nnnncnnnnn nnnnnnnnnn nncnnngnnn nnnngg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQYDYIIVG GGSGGSSLAG RLADACPDAT IALIEAGSHT ERNLLVNMPV GIAALVPFKL GTNYGYETVP QPGLGGRRGY QPRGRGMGGS SAINAMIYTR GHPGDYDEWA QLGATGWGWQ DVLPYFRRAE GNERGADAWH GADGPLTVSD LRFRNPFSER FIQAAHAAGY PLNDDFNGAT QEGVGFYQVT HRDGSRCSVA RAYIYGRNRP NLHVLTDATV LRVGFDGKRA VGVVVSRNGR VETLGARAEV ILSAGAFNSP QLLMCSGIGP AEQLRRHGIA VVQDAPDVGT NLIDHIDFII NTRVNSSELV GVCVRGIAKM TPALARYFSS RTGMMTSNVA EAGGFIKSDP SLARPDLQLH FCTALVDDHN RKMHWGFGYS LHVCALRPFS RGTVALASGD ARDAPLIDPR FFSDSRDLDL LVRGAQAMRR ILSQAPLASQ GGRELYTRPD QSEAELRATI VAHADTIYHP VGTCRMGSDA RAVVDPQLRV RGVDGLRVVD ASVMPTLVGG NTNAPSVMIG ERAADFIVAA RRGGVPRGEA ATAVHGR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaatacgac tacatcatcg tcggcggcgg ttcgggcggc tcgagcctgg ccggccgtct cgccgatgcc tgccccgatg cgacgatcgc actgatcgag gccggctcgc atacggagcg caacctgctg gtcaacatgc cggtcggcat cgcggcgctg gtgccgttca agctcggcac gaactacggc tacgagacgg tgccgcagcc gggcctcggc gggcggcgcg gttaccagcc gcgcgggcgc gggatgggcg gctcgagcgc gatcaacgcg atgatctata cgcgcggcca cccgggcgat tacgacgagt gggcgcagct cggcgcgacg ggctggggct ggcaggacgt gctgccgtat ttccgccgcg cggaaggcaa cgaacgcggc gccgacgcgt ggcacggcgc ggacggcccg ttgaccgttt ccgatctgcg ctttcgcaat ccgttctccg aacgattcat ccaggccgcg catgccgccg gctatccgct gaacgacgac ttcaacggcg cgacgcagga aggcgtcggc ttctatcagg tcacgcatcg cgacggttcg cgttgcagcg tggcgcgcgc gtatatctac ggccgcaacc ggccgaacct gcacgtgctc acggatgcga cggtgctgcg cgtcggcttc gacggcaagc gcgcggtcgg cgtcgtggtg tcgcgcaacg ggcgggtcga gacgctcggc gcacgcgcgg aagtgatcct gtcggccggc gcgttcaatt cgccgcaact gctgatgtgt tcgggaatcg gcccggcgga gcaactgcgc cgccacggga tcgcggtcgt gcaggatgcg ccggacgtcg gcacgaacct gatcgaccac atcgatttca tcatcaacac gcgcgtgaat tcgtcggagc tggtcggcgt gtgcgtgcgc ggcatcgcga agatgacgcc ggcgctcgcg cgctatttct cgagccgcac cgggatgatg acgagcaacg tcgcggaggc cggcggtttc atcaagagcg atccgtcgct cgcgcgtccc gacctgcaac tgcatttctg cacggcgctc gtcgacgacc acaaccgcaa gatgcactgg ggcttcggct attcgctgca cgtgtgcgcg ctgcggccgt tcagccgcgg cacggtcgcg ctcgcgagcg gcgatgcgcg cgacgcgccg ctgatcgatc cgcgcttctt cagcgattca cgcgatctcg acctgctggt gcgcggcgcg caggcgatgc gccgcatcct gtcgcaggcg ccgctcgcgt cgcagggcgg gcgcgagctg tacacgcggc cggaccagag cgaagcggag ctgcgcgcga cgatcgtcgc gcatgcggac acgatctatc acccggtcgg cacctgccgg atgggctccg atgcgcgcgc ggtcgtcgat ccgcaactgc gcgtgcgcgg cgtcgacgga ttgcgcgtgg tggatgcgtc ggtgatgccg acgctggtgg gcgggaacac gaatgcgccg tcggtgatga tcggcgaacg cgcggcggac ttcatcgtcg cggcgcgccg cggcggtgtg ccgcgcggcg aggcggctac cgcggtgcac ggccgctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa