BuceA.18077.a

Putative CheB family methylesterase

CENTER ID: BuceA.18077.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18077.a.A1.GE33249 full length 1 334
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.7340
RefSeq: YP_002154013.1
UniProt: B4EP25

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRRDIVVIA SSRGGLPVLR TLVAGIAADF PVPICIVQHI GRHRTILPEL LSRWGRLDAS EALHGESPEA GRIYVAPPDR HMIVRGGDLW LLDTAAENFA RPAADPLFRS AAREYGAGAI GVVLSGDLDD GAAGLAAIRA RGGYGIVQDP DECEAPSMPR SALAAAGADA VATAEELPRT LHAAVYGARA KEQRKMADLR DLDHESRIAE GGLVDPGLLD KIGTRSALTC PSCNGVLWRM RDDRPLRYRC HTGHAFSALS LDDAEAQKAE DAIWGAIRAV HERMIFARER QEWARRTGNA EDVAIEQARI DENQRLADVL RRAVGAALST GELE
NT Sequence
atggatcgtc gagatatcgt ggtaattgcg tcgtcccgag gcggtctccc ggtcctccgg acgttggtgg ccggcattgc cgcggatttt ccggtgccga tttgcatcgt gcagcacatt ggtcgacatc ggacgatcct gcccgagttg ttgtcgcgat ggggcaggct cgatgcaagc gaggcgctac acggggaatc gccggaggcg ggcagaattt acgtggcgcc gcccgaccgg cacatgatcg tgcgcggcgg cgacctgtgg ctgctcgata cggccgccga gaacttcgcc cgcccggccg ccgacccgct gtttcgctcc gccgcacgcg aatatggcgc cggtgcgatc ggcgtggtgc tcagcggcga cctcgatgac ggcgcggccg gcctggcggc cattcgcgca cggggcggct acggcatcgt tcaggacccc gatgaatgcg aagcgccgtc gatgccgcgc agcgcactgg ccgcggcggg agccgatgcg gtcgcgacgg cggaagaact gccgcggacc cttcatgcag cggtctacgg cgcacgtgcg aaggagcagc gaaaaatggc agatttgcgc gacctcgacc atgagagccg gattgccgag ggcggcctcg tcgatccggg actgcttgac aaaatcggca cgcgttccgc cctcacgtgc cccagttgca acggcgtgct ctggcgcatg cgcgacgatc gtccgttgcg ataccgttgc cataccgggc atgcgttctc cgcgctttcc ctcgacgacg cggaagccca gaaagcggag gatgcgatat ggggggcgat ccgcgcggtt cacgagcgca tgatttttgc gcgcgagcgc caggaatggg cgcgccgcac gggcaacgcc gaggacgtcg cgatcgaaca ggcccgcatc gacgaaaacc agcggctcgc ggacgtgctg cgtcgtgcgg tcggcgccgc actgagtacg ggcgaactgg agtga
Details for BuceA.18077.a.A1.GE33249
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 78
PERCENT COVERAGE: 83
Validated AA Sequence
MGTLEAQTQG PGSMDRRDIV VIASSRGGLP VLRTLVAGIA ADFPVPICIV QHIGRHRTIL PELLSRWGRL DASEALHGES PEAGRIYVAP PDRHMIVRGG DLWLLDTAAE NFARPAADPL FRSAAREYGA GAIGVVLSGD LDDGAAGLAA IRARGGYGIV QDPDECEAPS MPRSALAAAG ADAVATAEEL PRTLHAAVYG ARAKEQRKMA DLRDSXMRAG LPRAASSIRD CLTKSARVPP SRAPVATACS GACATIVRCD TVAIPGMRSP RFPSTTRKPR KRRXRYGGRS ARFTSA
Validated NT Sequence
gcnnatnacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggatcgtc gagatatcgt ggtaattgcg tcgtcccgag gcggtctccc ggtcctccgg acgttggtgg ccggcattgc cgcggatttt ccggtgccga tttgcatcgt gcagcacatt ggtcgacatc ggacgatcct gcccgagttg ttgtcgcgat ggggcaggct cgatgcaagc gaggcgctac acggggaatc gccggaggcg ggcagaattt acgtggcgcc gcccgaccgg cacatgatcg tgcgcggcgg cgacctgtgg ctgctcgata cggccgccga gaacttcgcc cgcccggccg ccgacccgct gtttcgctcc gccgcacgcg aatatggcgc cggtgcgatc ggcgtggtgc tcagcggcga cctcgatgac ggcgcggccg gcctggcggc cattcgcgca cggggcggct acggcatcgt tcaggacccc gatgaatgcg aagcgccgtc gatgccgcgc agcgcactgg ccgcggcggg agccgatgcg gtcgcgacgg cggaagaact gccgcggacc cttcatgcag cggtctacgg cgcacgtgcg aaggagcagc gaaaaatggc agatttgcgc gactcganca tgagagccgg attgccgagg gcggcctcgt cgatccggga ctgcttgaca aaatcggcac gcgttccgcc ctcacgtgcc ccagttgcaa cggcgtgctc tggcgcatgc gcgacgatcg tccgttgcga taccgttgcc ataccgggca tgcgttctcc gcgctttccc tcgacgacgc ggaagcccag aaagcggagg angcgatatg gggggcgatc cgcgcggttc acgagcgcat gatttttgcg cgcgagcgcc aggaatgggc gcgccncang ggcaacnccn agacgtcgng atcgaacagg cccgcatcga cnaaancagn gnnngnngnn ctgcgtcnng nngnnggnnn nnantganta nnnnnnnngg nntantaaan ngnnnnnnnn nnnncnnnnn ctnnnnnnnn nnnnngctng nngnngnnnn nnnnnnnnnn nnnnnnanna nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRRDIVVI ASSRGGLPVL RTLVAGIAAD FPVPICIVQH IGRHRTILPE LLSRWGRLDA SEALHGESPE AGRIYVAPPD RHMIVRGGDL WLLDTAAENF ARPAADPLFR SAAREYGAGA IGVVLSGDLD DGAAGLAAIR ARGGYGIVQD PDECEAPSMP RSALAAAGAD AVATAEELPR TLHAAVYGAR AKEQRKMADL RDLDHESRIA EGGLVDPGLL DKIGTRSALT CPSCNGVLWR MRDDRPLRYR CHTGHAFSAL SLDDAEAQKA EDAIWGAIRA VHERMIFARE RQEWARRTGN AEDVAIEQAR IDENQRLADV LRRAVGAALS TGELE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatcgtcga gatatcgtgg taattgcgtc gtcccgaggc ggtctcccgg tcctccggac gttggtggcc ggcattgccg cggattttcc ggtgccgatt tgcatcgtgc agcacattgg tcgacatcgg acgatcctgc ccgagttgtt gtcgcgatgg ggcaggctcg atgcaagcga ggcgctacac ggggaatcgc cggaggcggg cagaatttac gtggcgccgc ccgaccggca catgatcgtg cgcggcggcg acctgtggct gctcgatacg gccgccgaga acttcgcccg cccggccgcc gacccgctgt ttcgctccgc cgcacgcgaa tatggcgccg gtgcgatcgg cgtggtgctc agcggcgacc tcgatgacgg cgcggccggc ctggcggcca ttcgcgcacg gggcggctac ggcatcgttc aggaccccga tgaatgcgaa gcgccgtcga tgccgcgcag cgcactggcc gcggcgggag ccgatgcggt cgcgacggcg gaagaactgc cgcggaccct tcatgcagcg gtctacggcg cacgtgcgaa ggagcagcga aaaatggcag atttgcgcga cctcgaccat gagagccgga ttgccgaggg cggcctcgtc gatccgggac tgcttgacaa aatcggcacg cgttccgccc tcacgtgccc cagttgcaac ggcgtgctct ggcgcatgcg cgacgatcgt ccgttgcgat accgttgcca taccgggcat gcgttctccg cgctttccct cgacgacgcg gaagcccaga aagcggagga tgcgatatgg ggggcgatcc gcgcggttca cgagcgcatg atttttgcgc gcgagcgcca ggaatgggcg cgccgcacgg gcaacgccga ggacgtcgcg atcgaacagg cccgcatcga cgaaaaccag cggctcgcgg acgtgctgcg tcgtgcggtc ggcgccgcac tgagtacggg cgaactggag taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa