BuceA.18071.a

Putative LPS core biosynthesis protein

CENTER ID: BuceA.18071.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18071.a.A1.GE33247 full length 1 394
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2510
RefSeq: YP_002231527.1
UniProt: B4E6C9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTASLTFDTP PRSILVTCTR RIGDVLLATP LVRSLKRRWP DAQIDMVVFR GTEGVLEHNP DIRRVIVVAQ RAKPRERFAD AARLWRRYDL ACAAISSDRA RFYTWFGGRR RVGLVDPERI TRLTRFMLDG IALNRHGDVH TVTSSLAIAE VLGVTPCAEV VAPGIGTDPA RVAQFDALLY GPAALSRTRA HIVLHPSPMF RYKQWREDGW VELIRWARAS GFDVALSGGP GAAEVDYAQR IVAAAGEPVA NFVGRLTFGE TAELIRRARL FVGPDTGATH VAAACGTPTL ALFGPSNPVR WGPWPANWPA HDEPWALRGS AQRGNVYLMQ GDGDCVPCKL EGCDRHLESW SRCLTELSAQ RVIAAAAALV EGRPVVEAPR ESIIDVSALP RGGV
NT Sequence
gtgaccgctt cccttacgtt cgatacgccg ccgcgcagca ttctcgtcac ctgcacccgc aggatcggcg acgtgctgct ggcgacgccg ctggtccgct ccctgaagcg gcgctggcct gacgcgcaga tcgacatggt cgtgtttcgc ggcaccgagg gcgtgctcga gcacaatccc gacattcgtc gggtgatcgt cgtcgcgcag cgcgcgaagc cgcgcgagcg gttcgccgac gcggccaggc tgtggcggcg ctacgatctc gcatgcgcgg cgatcagctc cgatcgtgcc cgtttctata cgtggttcgg tgggcgtcgg cgcgtcgggc tcgtcgatcc ggagcggatc acgcggctga cgcgtttcat gctggacggc attgcgctca atcgccacgg cgatgtccat acggtgacga gcagtctcgc gattgcagaa gtgctcggcg tgacgccttg cgctgaagtg gtcgcgcccg gcatcggcac cgatccggcg cgcgtcgcgc aattcgacgc actgctgtat gggccggccg cgctgtcgcg tactcgagcg cacatcgtgc tgcacccatc cccgatgttt cgttacaagc agtggcgcga ggacggctgg gtcgaactga tccgctgggc ccgcgcaagc ggcttcgacg tcgcgctgag cggtgggcca ggcgcggcgg aggtcgacta tgcgcagcgg atcgtggcag ccgcgggcga gccggtcgcg aatttcgtcg ggcgcctgac gttcggcgag accgctgaac tgattcgccg cgcacggctg ttcgtcgggc cggacaccgg cgcgacgcac gtcgccgccg cgtgcgggac gccgacgctc gcgctgttcg ggccgtccaa cccggtgcgc tgggggccgt ggcccgccaa ctggcccgct cacgacgagc cgtgggcgct gcgcggttcg gcgcagcgcg gcaacgttta cctgatgcag ggtgacggtg actgcgtgcc ttgcaaactc gaaggctgcg accgtcatct ggaaagctgg agccgctgcc tgacggagct ttccgcgcag cgcgtgattg ccgccgcggc ggcgctcgtc gaaggccgtc cggtggtgga ggcgccgcgc gagtcgatca tcgacgtgtc ggccttgccg cgcggcggcg tataa
Details for BuceA.18071.a.A1.GE33247
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVTASLTFDT PPRSILVTCT RRIGDVLLAT PLVRSLKRRW PDAQIDMVVF RGTEGVLEHN PDIRRVIVVA QRAKPRERFA DAARLWRRYD LACAAISSDR ARFYTWFGGR RRVGLVDPER ITRLTRFMLD GIALNRHGDV HTVTSSLAIA EVLGVTPCAE VVAPGIGTDP ARVAQFDALL YGPAALSRTR AHIVLHPSPM FRYKQWREDG WVELIRWARA SGFDVALSGG PGAAEVDYAQ RIVAAAGEPV ANFVGRLTFG ETAELIRRAR LFVGPDTGAT HVAAACGTPT LALFGPSNPV RWGPWPANWP AHDEPWALRG SAQRGNVYLM QGDGDCVPCK LEGCDRHLES WSRCLTELSA QRVIAAAAAL VEGRPVVEAP RESIIDVSAL PRGGV
Validated NT Sequence
atacgccgcc gcgcggcaag gccgacacgt cgatgatcga ctcgcgcggc gcctccacca ccggacggcc ttcgacgagc gccgccgcgg cggcaatcac gcgctgcgcg gaaagctccg tcaggcagcg gctccagctt tccagatgac ggtcgcagcc ttcgagtttg caaggcacgc agtcaccgtc accctgcatc aggtaaacgt tgccgcgctg cgccgaaccg cgcagcgccc acggctcgtc gtgagcgggc cagttggcgg gccacggccc ccagcgcacc gggttggacg gcccgaacag cgcgagcgtc ggcgtcccgc acgcggcggc gacgtgcgtc gcgccggtgt ccggcccgac gaacagccgt gcgcggcgaa tcagttcagc ggtctcgccg aacgtcaggc gcccgacgaa attcgcgacc ggctcgcccg cggctgccac gatccgctgc gcatagtcga cctccgccgc gcctggccca ccgctcagcg cgacgtcgaa gccgcttgcg cgggcccagc ggatcagttc gacccagccg tcctcgcgcc actgcttgta acgaaacatc ggggatgggt gcagcacgat gtgcgctcga gtacgcgaca gcgcggccgg cccatacagc agtgcgtcga attgcgcgac gcgcgccgga tcggtgccga tgccgggcgc gaccacttca gcgcaaggcg tcacgccgag cacttctgca atcgcgagac tgctcgtcac cgtatggaca tcgccgtggc gattgagcgc aatgccgtcc agcatgaaac gcgtcagccg cgtgatccgc tccggatcga cgagcccgac gcgccgacgc ccaccgaacc acgtatagaa acgggcacga tcggagctga tcgccgcgca tgcgagatcg tagcgccgcc acagcctggc cgcgtcggcg aaccgctcgc gcggcttcgc gcgctgcgcg acgacgatca cccgacgaat gtcgggattg tgctcgagca cgccctcggt gccgcgaaac acgaccatgt cgatctgcgc gtcaggccag cgccgcttca gggagcggac cagcggcgtc gccagcagca cgtcgccgat cctgcgggtg caggtgacga gaatgctgcg cggcggcgta tcgaacgtaa gggaagcggt caccatcgaa ccaggaccct gggtctgagc ttccagggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTASLTFD TPPRSILVTC TRRIGDVLLA TPLVRSLKRR WPDAQIDMVV FRGTEGVLEH NPDIRRVIVV AQRAKPRERF ADAARLWRRY DLACAAISSD RARFYTWFGG RRRVGLVDPE RITRLTRFML DGIALNRHGD VHTVTSSLAI AEVLGVTPCA EVVAPGIGTD PARVAQFDAL LYGPAALSRT RAHIVLHPSP MFRYKQWRED GWVELIRWAR ASGFDVALSG GPGAAEVDYA QRIVAAAGEP VANFVGRLTF GETAELIRRA RLFVGPDTGA THVAAACGTP TLALFGPSNP VRWGPWPANW PAHDEPWALR GSAQRGNVYL MQGDGDCVPC KLEGCDRHLE SWSRCLTELS AQRVIAAAAA LVEGRPVVEA PRESIIDVSA LPRGGV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccgct tcccttacgt tcgatacgcc gccgcgcagc attctcgtca cctgcacccg caggatcggc gacgtgctgc tggcgacgcc gctggtccgc tccctgaagc ggcgctggcc tgacgcgcag atcgacatgg tcgtgtttcg cggcaccgag ggcgtgctcg agcacaatcc cgacattcgt cgggtgatcg tcgtcgcgca gcgcgcgaag ccgcgcgagc ggttcgccga cgcggccagg ctgtggcggc gctacgatct cgcatgcgcg gcgatcagct ccgatcgtgc ccgtttctat acgtggttcg gtgggcgtcg gcgcgtcggg ctcgtcgatc cggagcggat cacgcggctg acgcgtttca tgctggacgg cattgcgctc aatcgccacg gcgatgtcca tacggtgacg agcagtctcg cgattgcaga agtgctcggc gtgacgcctt gcgctgaagt ggtcgcgccc ggcatcggca ccgatccggc gcgcgtcgcg caattcgacg cactgctgta tgggccggcc gcgctgtcgc gtactcgagc gcacatcgtg ctgcacccat ccccgatgtt tcgttacaag cagtggcgcg aggacggctg ggtcgaactg atccgctggg cccgcgcaag cggcttcgac gtcgcgctga gcggtgggcc aggcgcggcg gaggtcgact atgcgcagcg gatcgtggca gccgcgggcg agccggtcgc gaatttcgtc gggcgcctga cgttcggcga gaccgctgaa ctgattcgcc gcgcacggct gttcgtcggg ccggacaccg gcgcgacgca cgtcgccgcc gcgtgcggga cgccgacgct cgcgctgttc gggccgtcca acccggtgcg ctgggggccg tggcccgcca actggcccgc tcacgacgag ccgtgggcgc tgcgcggttc ggcgcagcgc ggcaacgttt acctgatgca gggtgacggt gactgcgtgc cttgcaaact cgaaggctgc gaccgtcatc tggaaagctg gagccgctgc ctgacggagc tttccgcgca gcgcgtgatt gccgccgcgg cggcgctcgt cgaaggccgt ccggtggtgg aggcgccgcg cgagtcgatc atcgacgtgt cggccttgcc gcgcggcggc gtataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa