BuceA.18069.a

Catalase-peroxidase 1 (CP 1) (EC 1.11.1.6) (EC 1.11.1.7) (Peroxidase/catalase 1)

CENTER ID: BuceA.18069.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18069.a.A1.GE33246 full length 1 728
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3422
RefSeq: YP_002232403.1
UniProt: B4EE21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNETKCPFN HTAGSGTTNK DWWPNQLNLN VLHRHSALSD PMDPDFDYAE AFKKLDLAAV KQDLHALMTT SQDWWPADFG HYGGLFVRMA WHSAGTYRTA DGRGGAGGGQ QRFAPLNSWP DNVSLDKARR LLWPIKQKYG RNISWADLLI LTGNVALESM GFKTFGYAGG RVDTWEPDDV YWGSEKIWLE LSGGPNSRYT GKRELESPLA AVQMGLIYVN PEGPDGNPDP VAAAHDIRET FARMAMNDEE TVALIAGGHT FGKTHGAGPA SNVGPEPEAA GLEEQGLGWK STFGTGKGKD TITSGLEVTW TSTPTKWSND FFKHLFSYEW ELTKSPAGAH QWVAKDADEV IPDAYDASKK HRPTMLTTDL SLRFDPAYEK ISRRFYENPA EFADAFARAW FKLTHRDMGP RSRYLGPEVP AEHLLWQDPI PAVDHPLIDD ADVAALKAKV LATGLSVSQL VSTAWASAAT FRGSDKRGGA NGARIRLAPQ KDWEVNQPAA LAAVLEALEG VQKAFNDAQT GGKKVSLADL IVLAGAAGVE QAAKNAGIAI TVPFAPGRMD ASQEETDVDA MAVLEPLADG FRNYLKHPYK TPAEALLVDK AQLLTLTAPE MTVLVGGLRV LGANVGDSKH GVFTDRPGTL SNDFFANLLD MRTEWKPVSA ANDVFEGRDR ATGAVKWTGT RVDLIFGSHS QLRALAEVYG SADAQEKFVR DFVAAWNKVM NLDRFDLA
NT Sequence
atgtcgaacg aaaccaagtg cccgttcaac cacaccgcag gcagcggcac gacgaacaag gactggtggc cgaatcagct gaacctcaac gtcctgcatc ggcattcggc gctgtccgat ccgatggacc cggatttcga ctacgccgag gcgttcaaga agctggatct cgccgcggtg aagcaggacc tgcacgcgct gatgacgacg tcgcaggact ggtggccggc cgacttcggc cactacggcg gtctgttcgt gcgcatggcg tggcatagcg cgggcacgta ccgcacggcc gacggccgcg gcggcgcggg cggcgggcag cagcgcttcg cgccgctcaa cagctggccg gacaacgtga gcctcgacaa ggcgcgccgc ctgctgtggc cgatcaagca gaagtacggc cgcaacatct cgtgggccga cctgctgatc ctgaccggca acgtcgcgct cgaatcgatg ggcttcaaga cgttcggcta tgccggcggc cgcgtcgaca cgtgggaacc ggacgacgtc tactggggct cggaaaagat ctggctggaa ctgagcggcg gcccgaacag ccgctacacg ggcaagcgcg agctcgaaag cccgctcgcc gcggtgcaga tgggcctgat ctacgtgaac ccggaaggtc cggacggcaa ccccgacccg gtcgccgccg cgcacgacat ccgcgagacg ttcgcgcgga tggcgatgaa cgacgaggaa acggtcgcgc tgatcgcggg cggtcacacg ttcggcaaga cgcacggcgc cggcccggcc tcgaacgtcg gccccgagcc ggaagccgcg ggcctcgagg agcaggggct cggctggaag agcaccttcg gcaccggcaa gggcaaggac acgatcacga gcggcctcga agtcacgtgg acctcgacgc cgacgaagtg gagcaacgac ttcttcaagc acctgttcag ctacgagtgg gagctcacga agagcccggc cggcgcgcac cagtgggtcg cgaaggatgc ggacgaggtg attcccgatg cgtacgacgc gtcgaagaag catcgcccga cgatgctgac gaccgacctg tcgctgcgtt tcgacccggc ctacgaaaag atctcgcgcc gcttctacga gaacccggcc gaattcgccg acgcattcgc acgtgcgtgg ttcaagctga cgcaccgtga catgggtccg cgctcgcgct acctcggccc ggaagtgccg gcggaacacc tgctgtggca ggacccgatc ccggccgtcg atcatccgct gatcgacgac gccgacgtcg ccgcgctgaa ggcgaaggtg ctggccacgg gcctgtcggt gtcgcagctc gtgtcgaccg cctgggcgtc cgcggcgacg ttccgcgggt cggacaagcg cggtggcgcg aacggcgcgc gcatccgcct cgcgccgcag aaggactggg aagtgaacca gccggcggcg ctcgcggcgg tactcgaagc gctcgaaggc gtgcagaagg cgttcaacga tgcgcagacg ggcggcaaga aggtgtcgct ggccgacctg atcgtgctgg ccggtgcggc cggcgtcgag caggcggcga agaacgcggg catcgcgatc acggtgccgt tcgcgccggg ccggatggat gcgtcgcagg aagaaaccga cgtcgacgcg atggccgtgc tcgagccgct cgccgacggg ttccgcaact acctgaagca cccgtacaag acgccggccg aggcgctgct ggtcgacaag gcgcaactgc tgacgttgac ggcgccggag atgaccgtgc tggtcggcgg cttgcgcgtg ctcggcgcga acgtcggcga ctcgaagcac ggcgtgttca ccgatcgtcc gggcacgctg tcgaacgact tcttcgcgaa cctgctcgac atgcgcacgg aatggaagcc ggtgtcggcc gcgaacgacg tgttcgaagg gcgcgatcgc gcgacgggcg cggtcaagtg gacgggcacg cgcgtcgacc tgatcttcgg ctcgcactcg caactgcgcg cgctggccga ggtgtacggc agcgcggatg cgcaggagaa gttcgtgcgc gacttcgtcg ccgcctggaa caaggtgatg aacctcgacc gcttcgacct cgcatga
Details for BuceA.18069.a.A1.GE33246
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 81kDa
OBSERVED MW: 81kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 52
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSNETKCPF NHTAGSGTTN KDWWPNQLNL NVLHRHSALS DPMDPDFDYA EAFKKLDLAA VKQDLHALMT TSQDWWPADF GHYGGLFVRM AWHSAGTYRT ADGRGGAGGG QQRFAPLNSW PDNVSLDKAR RLLWPIKQKY GRNISWADLL ILTGNVALES MGFKTFGYAG GRVDTWEPDD VYWGSEKIWL ELSGGPNSRY TGKRELESPL AAVQMGLIYV NPEGPDGNPD PVAAAHDIRE TFARMAMNDE ETVALIAGGH TFGKTHGAXP ASNVGPXPEA AGLXEQGSAG RXPSAPXXXD TXXXGXXXXX XRRXXXXXXX SXXXXLXXGX XXXXXXXXXG XXXXXXXXXX TXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
cnnnnnnnnn nnataatttt nnttaacttt aaganggaga tataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgtcgaacga aaccaagtgc ccgttcaacc acaccgcagg cagcggcacg acgaacaagg actggtggcc gaatcagctg aacctcaacg tcctgcatcg gcattcggcg ctgtccgatc cgatggaccc ggatttcgac tacgccgagg cgttcaagaa gctggatctc gccgcggtga agcaggacct gcacgcgctg atgacgacgt cgcaggactg gtggccggcc gacttcggcc actacggcgg tctgttcgtg cgcatggcgt ggcatagcgc gggcacgtac cgcacggccg acggccgcgg cggcgcgggc ggcgggcagc agcgcttcgc gccgctcaac agctggccgg acaacgtgag cctcgacaag gcgcgccgcc tgctgtggcc gatcaagcag aagtacggcc gcaacatctc gtgggccgac ctgctgatcc tgaccggcaa cgtcgcgctc gaatcgatgg gcttcaagac gttcggctat gccggcggcc gcgtcgacac gtgggaaccg gacgacgtct actggggctc ggaaaagatc tggctggaac tgagcggcgg cccgaacagc cgctacacgg gcaagcgcga gctcgaaagc ccgctcgccg cggtgcagat gggcctgatc tacgtgaacc cggaaggtcc ggacggcaac cccgacccgg tcgccgccgc gcacgacatc cgcgagacgt tcgcgcggat ggcgatgaac gacgaggaaa cggtcgcgct gatcgcgggc ggtcacacgt tcggcaagac gcacggcgcc cncccngcct cnaacgtcgg ccccganccg gaagccgcgg gcctcnagga gcagggctcg gctggaagan caccttcggc accggnnngg nnngacacga tnnnnancgg nntcnaantc ncntnnnctc gacgccnaaa nnnnncnncn annnnntcnn gcnnnnntna nctncnannn ggnanctnnn nnnnnnnnnn nnnnnnnnnc nntggnnnnn nnnngnnnnn annnnntncn nnncnnacna nnnnnnnnnn nntcnnnnnn nannnnnann acnnncnnnn cnnnncnnnn nnnnnncnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNETKCPF NHTAGSGTTN KDWWPNQLNL NVLHRHSALS DPMDPDFDYA EAFKKLDLAA VKQDLHALMT TSQDWWPADF GHYGGLFVRM AWHSAGTYRT ADGRGGAGGG QQRFAPLNSW PDNVSLDKAR RLLWPIKQKY GRNISWADLL ILTGNVALES MGFKTFGYAG GRVDTWEPDD VYWGSEKIWL ELSGGPNSRY TGKRELESPL AAVQMGLIYV NPEGPDGNPD PVAAAHDIRE TFARMAMNDE ETVALIAGGH TFGKTHGAGP ASNVGPEPEA AGLEEQGLGW KSTFGTGKGK DTITSGLEVT WTSTPTKWSN DFFKHLFSYE WELTKSPAGA HQWVAKDADE VIPDAYDASK KHRPTMLTTD LSLRFDPAYE KISRRFYENP AEFADAFARA WFKLTHRDMG PRSRYLGPEV PAEHLLWQDP IPAVDHPLID DADVAALKAK VLATGLSVSQ LVSTAWASAA TFRGSDKRGG ANGARIRLAP QKDWEVNQPA ALAAVLEALE GVQKAFNDAQ TGGKKVSLAD LIVLAGAAGV EQAAKNAGIA ITVPFAPGRM DASQEETDVD AMAVLEPLAD GFRNYLKHPY KTPAEALLVD KAQLLTLTAP EMTVLVGGLR VLGANVGDSK HGVFTDRPGT LSNDFFANLL DMRTEWKPVS AANDVFEGRD RATGAVKWTG TRVDLIFGSH SQLRALAEVY GSADAQEKFV RDFVAAWNKV MNLDRFDLA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgaacgaa accaagtgcc cgttcaacca caccgcaggc agcggcacga cgaacaagga ctggtggccg aatcagctga acctcaacgt cctgcatcgg cattcggcgc tgtccgatcc gatggacccg gatttcgact acgccgaggc gttcaagaag ctggatctcg ccgcggtgaa gcaggacctg cacgcgctga tgacgacgtc gcaggactgg tggccggccg acttcggcca ctacggcggt ctgttcgtgc gcatggcgtg gcatagcgcg ggcacgtacc gcacggccga cggccgcggc ggcgcgggcg gcgggcagca gcgcttcgcg ccgctcaaca gctggccgga caacgtgagc ctcgacaagg cgcgccgcct gctgtggccg atcaagcaga agtacggccg caacatctcg tgggccgacc tgctgatcct gaccggcaac gtcgcgctcg aatcgatggg cttcaagacg ttcggctatg ccggcggccg cgtcgacacg tgggaaccgg acgacgtcta ctggggctcg gaaaagatct ggctggaact gagcggcggc ccgaacagcc gctacacggg caagcgcgag ctcgaaagcc cgctcgccgc ggtgcagatg ggcctgatct acgtgaaccc ggaaggtccg gacggcaacc ccgacccggt cgccgccgcg cacgacatcc gcgagacgtt cgcgcggatg gcgatgaacg acgaggaaac ggtcgcgctg atcgcgggcg gtcacacgtt cggcaagacg cacggcgccg gcccggcctc gaacgtcggc cccgagccgg aagccgcggg cctcgaggag caggggctcg gctggaagag caccttcggc accggcaagg gcaaggacac gatcacgagc ggcctcgaag tcacgtggac ctcgacgccg acgaagtgga gcaacgactt cttcaagcac ctgttcagct acgagtggga gctcacgaag agcccggccg gcgcgcacca gtgggtcgcg aaggatgcgg acgaggtgat tcccgatgcg tacgacgcgt cgaagaagca tcgcccgacg atgctgacga ccgacctgtc gctgcgtttc gacccggcct acgaaaagat ctcgcgccgc ttctacgaga acccggccga attcgccgac gcattcgcac gtgcgtggtt caagctgacg caccgtgaca tgggtccgcg ctcgcgctac ctcggcccgg aagtgccggc ggaacacctg ctgtggcagg acccgatccc ggccgtcgat catccgctga tcgacgacgc cgacgtcgcc gcgctgaagg cgaaggtgct ggccacgggc ctgtcggtgt cgcagctcgt gtcgaccgcc tgggcgtccg cggcgacgtt ccgcgggtcg gacaagcgcg gtggcgcgaa cggcgcgcgc atccgcctcg cgccgcagaa ggactgggaa gtgaaccagc cggcggcgct cgcggcggta ctcgaagcgc tcgaaggcgt gcagaaggcg ttcaacgatg cgcagacggg cggcaagaag gtgtcgctgg ccgacctgat cgtgctggcc ggtgcggccg gcgtcgagca ggcggcgaag aacgcgggca tcgcgatcac ggtgccgttc gcgccgggcc ggatggatgc gtcgcaggaa gaaaccgacg tcgacgcgat ggccgtgctc gagccgctcg ccgacgggtt ccgcaactac ctgaagcacc cgtacaagac gccggccgag gcgctgctgg tcgacaaggc gcaactgctg acgttgacgg cgccggagat gaccgtgctg gtcggcggct tgcgcgtgct cggcgcgaac gtcggcgact cgaagcacgg cgtgttcacc gatcgtccgg gcacgctgtc gaacgacttc ttcgcgaacc tgctcgacat gcgcacggaa tggaagccgg tgtcggccgc gaacgacgtg ttcgaagggc gcgatcgcgc gacgggcgcg gtcaagtgga cgggcacgcg cgtcgacctg atcttcggct cgcactcgca actgcgcgcg ctggccgagg tgtacggcag cgcggatgcg caggagaagt tcgtgcgcga cttcgtcgcc gcctggaaca aggtgatgaa cctcgaccgc ttcgacctcg cataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa