SSGCID: Target Page

BuceA.18062.a

DNA topoisomerase (EC 5.99.1.2)

CENTER ID:	BuceA.18062.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18062.a.A2.GE33244	domain 1		1	611
BuceA.18062.a.A3.GE33245	domain 2		612	865

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18062.a.A2.PW33854	domain 1		1	611
BuceA.18062.a.A3.PW33855	domain 2		612	865

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.481
RefSeq:	YP_002229624.1
UniProt:	B4E7V4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKALIIAEK PSVANDIARA LGGFTKHDEY YESDDFVLSS AVGHLLEIAA PEEYEVKRGK WSFAHLPVIP PHFDLNPIAK SESRLKVLTK LMKRKDVDRL INACDAGREG ELIFRLIAQH AKAKQPVQRL WLQSMTPQAI RDGFANLRSD SDMQPLADAA RCRSEADWLV GINGTRAMTA FNSKGGGFFL TTVGRVQTPT LSIVVEREEK IRRFIPRDYW EVKAEFACAG GFYEGKWFDP KFKRDEFDPE KRDSRLWSLP AAETIVAACR DQVGTVSEES KPSTQLSPLL FDLTSLQREA NSRFGFSAKN TLGLAQALYE KHKVLTYPRT DARALPEDYL STVQSTLEML KESHNYLPHA KQVLDKGWVK PNKRIFDNSK ISDHFAIIPT LQAPKSLSEP EQKLYDMVVK RFLAVFFPAA EFRVTTRITE VAGHHFKTEG KVLVEPGWLQ VYGRDAEGAD ANLVPVQKDE KVKTDEIAAV ALVTKPPARY SEATLLSAME GAGKLVEDDE LREAMAAKGL GTPATRAAII EGLLGEKYLL REGRELIPTA KAFQLMTLLR GLGVKELTAP ELTGEWEYKL SQMERGNLGR DAFMQEIARM TQQIVKRAKE YDSDTIPGDY ATLETPCPNC GGQVKENYRR FACTKCDFSI SKIPGSRQFE IPEVEELLQK KEIGPLSGFR SKMGRPFSAI LKLSFDDETK NYKLEFDFGQ DQGGEEGEAP DFSAQEPVGA CPKCKGRVFE HGMSYVCEHS VANPKTCDFR SGKVILQQEI TREQMGKLLA DGRTDLLPNF KSSRTGRNFK AFLVKQPDGK IGFEFEKKEP KAAAAKKTAK SATKDAETVT EGAEEKPAPA RKTAARKTTA RKTGS

NT Sequence

atgtccaaag cactgatcat tgcggaaaag ccttctgtcg cgaacgacat cgcacgcgct ttgggcggct ttaccaagca tgacgagtat tacgagagcg acgatttcgt cctttcgtcc gctgtcggcc acctgctgga aatcgccgcc ccggaagagt acgaggtcaa gcgcgggaaa tggagcttcg cgcatcttcc cgtcattccg ccgcatttcg acctgaaccc gatcgcaaaa agcgagtcgc gcctcaaggt gctcacgaag ctgatgaagc gcaaggacgt cgaccgcctg atcaacgcat gtgacgcggg gcgcgaaggc gagctgattt tccgcctgat cgcgcagcac gcgaaggcca agcagccggt ccagcgcctg tggctgcaat cgatgacgcc gcaggcgatc cgcgacggtt tcgcgaacct gcgcagcgac tccgacatgc agccgcttgc cgacgccgca cgctgccgct cggaagccga ctggctcgtc gggatcaacg gcacccgcgc gatgaccgcg ttcaacagca agggcggcgg cttcttcctg accaccgtcg gtcgcgttca gacgccaacg ttgtcgatcg tcgtcgaacg cgaagagaaa atccgccgct tcatcccgcg cgactactgg gaagtgaaag ccgaattcgc gtgcgcgggc ggcttctacg aaggcaagtg gttcgacccg aaattcaagc gcgacgaatt cgatccggaa aagcgcgatt cccgcctgtg gagcctgccg gccgccgaaa cgatcgtcgc cgcctgccgc gaccaggtcg gcacggtgtc cgaggaatcg aagccgtcga cgcagctgtc gccgctgctg ttcgacctga cgagcctgca gcgcgaggcg aacagccgct tcggcttctc cgcgaagaat acgctcggcc tcgcccaggc gctgtatgaa aagcacaagg tgctgaccta cccgcgtacc gacgcgcgcg cgctgccgga agactacctg tcgacggtcc agtccacgct cgagatgctc aaggagagcc acaactacct gccgcatgcc aagcaggtgc tcgacaaggg ctgggtgaag ccgaacaagc ggatcttcga caactcgaag atcagcgatc actttgccat catcccgacg ctgcaggcgc cgaagtcgct gtccgagccg gagcagaagc tgtacgacat ggtcgtgaag cgcttcctcg cggtgttctt cccggccgcc gaattccgcg tgacgacgcg catcaccgaa gtcgccgggc atcacttcaa gaccgaaggc aaggtgctcg tcgaaccggg ctggctgcag gtgtacggcc gcgacgccga aggcgcggac gcgaacctcg tgccggtgca gaaggacgag aaggtgaaaa cggacgaaat cgccgccgtc gcgctcgtga ccaagccgcc cgcacgctac tcggaagcga cgctgctgtc cgcgatggaa ggcgccggca agctggtcga ggacgacgag ttgcgcgagg cgatggccgc gaaaggtctc ggcacgccgg cgacgcgcgc ggcgatcatc gaaggcctgc tcggagagaa gtacctgctg cgcgagggcc gcgaactgat cccgaccgcg aaggcattcc agctgatgac gctgctgcgc ggcctcggcg tgaaggagct caccgcgccc gagctcaccg gcgaatggga atacaagctg tcgcagatgg agcgcggcaa tctcggccgc gacgcgttca tgcaggaaat cgcccgcatg acgcagcaga tcgtcaagcg cgcgaaggaa tacgattccg acacgattcc cggcgactac gcaacgctcg agacgccgtg cccgaactgc ggcggccagg tgaaggaaaa ctaccgccgc ttcgcgtgca cgaagtgcga cttctcgatc tcgaagatcc cgggcagccg gcagttcgag attccggaag tcgaggagct gctgcagaag aaggaaatcg ggccgctgtc cgggttccgc agcaagatgg ggcgcccgtt ctccgcgatc ctcaagctgt ccttcgacga cgagacgaag aactacaagc tcgagttcga tttcggccag gaccagggcg gcgaggaagg cgaagcgccc gacttctccg cgcaggaacc ggtcggcgcg tgcccgaagt gcaagggccg cgtgttcgag cacgggatga gctacgtgtg cgagcactcg gtcgcgaacc cgaagacctg cgacttccgc tcgggcaagg tgatcctgca acaggaaatc acgcgcgagc agatgggcaa gctgctggcc gacggccgca cggatctgct gccgaacttc aagtcgtcgc gcaccggccg caacttcaag gcgttcctcg tgaagcagcc ggacggcaag atcggtttcg agttcgagaa gaaggagccg aaagccgccg ccgcgaagaa gacggccaaa tcggcgacga aggacgccga aaccgtgacg gaaggcgccg aggagaaacc ggcaccggcc cgcaagaccg ccgcccgcaa aaccacggcg cgcaagacgg gttcgtga

Details for BuceA.18062.a.A2.GE33244

HARVESTED ON:	8/30/2011
SEQUENCED ON:	9/2/2011
EXPECTED MW:	70kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	78
PERCENT COVERAGE:	59

Validated AA Sequence

MSKALIIAEK PSVANDIARA LGGFTKHDEY YESDDFVLSS AVGHLLEIAA PEEYEVKRGK WSFAHLPVIP PHFDLNPIAK SESRLXVLTK LMKRKDVDRL INACDAGREG ELIFRLIAQH AKAKQPVQRL WLQSMTPQAI RDGFANLRSD SDMQPLADAA RCRSEADWLV GINGTRAMTA FNSKGGGFFL TTVGRVQTPT LSIVVEREEK IRRFIPRDYX EVKAEFACAG GFYEGKWFDP KFKRDEXDPE KRDSRLWSXP AAETIVAAXR DQVGTVSXES KPSTQLXPLL FDLTSXXREA NXXFXXXXXX ARPXPXRXXK XXLXXPXXXX XAXXXXPXXX XXSXXXXXXX XXXXXXXXGX XXXXXXXXXX XXXXXXXXX

Validated NT Sequence

annnnnnngn taccctggaa gctcagaccc agggtcctgg ttcgatgtcc aaagcactga tcattgcgga aaagccttct gtcgcgaacg acatcgcacg cgctttgggc ggctttacca agcatgacga gtattacgag agcgacgatt tcgtcctttc gtccgctgtc ggccacctgc tggaaatcgc cgccccggaa gagtacgagg tcaagcgcgg gaaatggagc ttcgcgcatc ttcccgtcat tccgccgcat ttcgacctga acccgatcgc aaaaagcgag tcgcgcctca nggtgctcac gaagctgatg aagcgcaagg acgtcgaccg cctgatcaac gcatgtgacg cggggcgcga aggcgagctg attttccgcc tgatcgcgca gcacgcgaag gccaagcagc cggtccagcg cctgtggctg caatcgatga cgccgcaggc gatccgcgac ggtttcgcga acctgcgcag cgactccgac atgcagccgc ttgccgacgc cgcacgctgc cgctcggaag ccgactggct cgtcgggatc aacggcaccc gcgcgatgac cgcgttcaac agcaagggcg gcggcttctt cctgaccacc gtcggtcgcg ttcagacgcc aacgttgtcg atcgtcgtcg aacgcgaaga gaaaatccgc cgcttcatcc cgcgcgacta cngggaagtg aaagccgaat tcgcgtgcgc gggcggcttc tacgaaggca agtggttcga cccgaaattc aagcgcgacg aanncgatcc ggaaaagcgc gattcccgcc tgtggagccn gccggccgcc gaaacgatcg tcgccgccng ccgcgaccag gtcggcacgg tgtccnanga atcnaagccg tcnacgcagc tnncgccnct gctgttcgac ctgacnagcn ngcancgcga ggcgaacagn nncttcgnnt nnnnnnnaan anacgctcgg cctcncccnn ggcgctntan naaancanng ctganntnnc cgnnnncnnn nnnnnnngcn cnncnnannn tnccnnngnn nnncnnngnn tcnannngcn nnnnnnnnnn nnnnntacna nnnnnnnnnn nnnnnnnngg nnnnnnnnnn nnanannngn nnnnnannnn nnannnnnnn nnnntnnncc nnannnnnnn nnng

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSKALIIAE KPSVANDIAR ALGGFTKHDE YYESDDFVLS SAVGHLLEIA APEEYEVKRG KWSFAHLPVI PPHFDLNPIA KSESRLKVLT KLMKRKDVDR LINACDAGRE GELIFRLIAQ HAKAKQPVQR LWLQSMTPQA IRDGFANLRS DSDMQPLADA ARCRSEADWL VGINGTRAMT AFNSKGGGFF LTTVGRVQTP TLSIVVEREE KIRRFIPRDY WEVKAEFACA GGFYEGKWFD PKFKRDEFDP EKRDSRLWSL PAAETIVAAC RDQVGTVSEE SKPSTQLSPL LFDLTSLQRE ANSRFGFSAK NTLGLAQALY EKHKVLTYPR TDARALPEDY LSTVQSTLEM LKESHNYLPH AKQVLDKGWV KPNKRIFDNS KISDHFAIIP TLQAPKSLSE PEQKLYDMVV KRFLAVFFPA AEFRVTTRIT EVAGHHFKTE GKVLVEPGWL QVYGRDAEGA DANLVPVQKD EKVKTDEIAA VALVTKPPAR YSEATLLSAM EGAGKLVEDD ELREAMAAKG LGTPATRAAI IEGLLGEKYL LREGRELIPT AKAFQLMTLL RGLGVKELTA PELTGEWEYK LSQMERGNLG RDAFMQEIAR MTQQIVKRAK EY

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccaaagca ctgatcattg cggaaaagcc ttctgtcgcg aacgacatcg cacgcgcttt gggcggcttt accaagcatg acgagtatta cgagagcgac gatttcgtcc tttcgtccgc tgtcggccac ctgctggaaa tcgccgcccc ggaagagtac gaggtcaagc gcgggaaatg gagcttcgcg catcttcccg tcattccgcc gcatttcgac ctgaacccga tcgcaaaaag cgagtcgcgc ctcaaggtgc tcacgaagct gatgaagcgc aaggacgtcg accgcctgat caacgcatgt gacgcggggc gcgaaggcga gctgattttc cgcctgatcg cgcagcacgc gaaggccaag cagccggtcc agcgcctgtg gctgcaatcg atgacgccgc aggcgatccg cgacggtttc gcgaacctgc gcagcgactc cgacatgcag ccgcttgccg acgccgcacg ctgccgctcg gaagccgact ggctcgtcgg gatcaacggc acccgcgcga tgaccgcgtt caacagcaag ggcggcggct tcttcctgac caccgtcggt cgcgttcaga cgccaacgtt gtcgatcgtc gtcgaacgcg aagagaaaat ccgccgcttc atcccgcgcg actactggga agtgaaagcc gaattcgcgt gcgcgggcgg cttctacgaa ggcaagtggt tcgacccgaa attcaagcgc gacgaattcg atccggaaaa gcgcgattcc cgcctgtgga gcctgccggc cgccgaaacg atcgtcgccg cctgccgcga ccaggtcggc acggtgtccg aggaatcgaa gccgtcgacg cagctgtcgc cgctgctgtt cgacctgacg agcctgcagc gcgaggcgaa cagccgcttc ggcttctccg cgaagaatac gctcggcctc gcccaggcgc tgtatgaaaa gcacaaggtg ctgacctacc cgcgtaccga cgcgcgcgcg ctgccggaag actacctgtc gacggtccag tccacgctcg agatgctcaa ggagagccac aactacctgc cgcatgccaa gcaggtgctc gacaagggct gggtgaagcc gaacaagcgg atcttcgaca actcgaagat cagcgatcac tttgccatca tcccgacgct gcaggcgccg aagtcgctgt ccgagccgga gcagaagctg tacgacatgg tcgtgaagcg cttcctcgcg gtgttcttcc cggccgccga attccgcgtg acgacgcgca tcaccgaagt cgccgggcat cacttcaaga ccgaaggcaa ggtgctcgtc gaaccgggct ggctgcaggt gtacggccgc gacgccgaag gcgcggacgc gaacctcgtg ccggtgcaga aggacgagaa ggtgaaaacg gacgaaatcg ccgccgtcgc gctcgtgacc aagccgcccg cacgctactc ggaagcgacg ctgctgtccg cgatggaagg cgccggcaag ctggtcgagg acgacgagtt gcgcgaggcg atggccgcga aaggtctcgg cacgccggcg acgcgcgcgg cgatcatcga aggcctgctc ggagagaagt acctgctgcg cgagggccgc gaactgatcc cgaccgcgaa ggcattccag ctgatgacgc tgctgcgcgg cctcggcgtg aaggagctca ccgcgcccga gctcaccggc gaatgggaat acaagctgtc gcagatggag cgcggcaatc tcggccgcga cgcgttcatg caggaaatcg cccgcatgac gcagcagatc gtcaagcgcg cgaaggaata ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for BuceA.18062.a.A3.GE33245

HARVESTED ON:	8/30/2011
SEQUENCED ON:	9/2/2011
EXPECTED MW:	30kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDSDTIPGD YATLETPCPN CGGQVKENYR RFACTKCDFS ISKIPGSRQF EIPEVEELLQ KKEIGPLSGF RSKMGRPFSA ILKLSFDDET KNYKLEFDFG QDQGGEEGEA PDFSAQEPVG ACPKCKGRVF EHGMSYVCEH SVANPKTCDF RSGKVILQQE ITREQMGKLL ADGRTDLLPN FKSSRTGRNF KAFLVKQPDG KIGFEFEKKE PKAAAAKKTA KSATKDAETV TEGAEEKPAP ARKTAARKTT ARKTGS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggattccgac acgattcccg gcgactacgc aacgctcgag acgccgtgcc cgaactgcgg cggccaggtg aaggaaaact accgccgctt cgcgtgcacg aagtgcgact tctcgatctc gaagatcccg ggcagccggc agttcgagat tccggaagtc gaggagctgc tgcagaagaa ggaaatcggg ccgctgtccg ggttccgcag caagatgggg cgcccgttct ccgcgatcct caagctgtcc ttcgacgacg agacgaagaa ctacaagctc gagttcgatt tcggccagga ccagggcggc gaggaaggcg aagcgcccga cttctccgcg caggaaccgg tcggcgcgtg cccgaagtgc aagggccgcg tgttcgagca cgggatgagc tacgtgtgcg agcactcggt cgcgaacccg aagacctgcg acttccgctc gggcaaggtg atcctgcaac aggaaatcac gcgcgagcag atgggcaagc tgctggccga cggccgcacg gatctgctgc cgaacttcaa gtcgtcgcgc accggccgca acttcaaggc gttcctcgtg aagcagccgg acggcaagat cggtttcgag ttcgagaaga aggagccgaa agccgccgcc gcgaagaaga cggccaaatc ggcgacgaag gacgccgaaa ccgtgacgga aggcgccgag gagaaaccgg caccggcccg caagaccgcc gcccgcaaaa ccacggcgcg caagacgggt tcgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BuceA.18062.a.A2.PW33854

PURIFICATION DATe:	2/26/2012
CONCENTRATION:	37.36mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	110µl
PERCENT IDENTITY:	78
PERCENT COVERAGE:	59

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCAAAGCA CTGATCATTG CGGAAAAGCC TTCTGTCGCG AACGACATCG CACGCGCTTT GGGCGGCTTT ACCAAGCATG ACGAGTATTA CGAGAGCGAC GATTTCGTCC TTTCGTCCGC TGTCGGCCAC CTGCTGGAAA TCGCCGCCCC GGAAGAGTAC GAGGTCAAGC GCGGGAAATG GAGCTTCGCG CATCTTCCCG TCATTCCGCC GCATTTCGAC CTGAACCCGA TCGCAAAAAG CGAGTCGCGC CTCAAGGTGC TCACGAAGCT GATGAAGCGC AAGGACGTCG ACCGCCTGAT CAACGCATGT GACGCGGGGC GCGAAGGCGA GCTGATTTTC CGCCTGATCG CGCAGCACGC GAAGGCCAAG CAGCCGGTCC AGCGCCTGTG GCTGCAATCG ATGACGCCGC AGGCGATCCG CGACGGTTTC GCGAACCTGC GCAGCGACTC CGACATGCAG CCGCTTGCCG ACGCCGCACG CTGCCGCTCG GAAGCCGACT GGCTCGTCGG GATCAACGGC ACCCGCGCGA TGACCGCGTT CAACAGCAAG GGCGGCGGCT TCTTCCTGAC CACCGTCGGT CGCGTTCAGA CGCCAACGTT GTCGATCGTC GTCGAACGCG AAGAGAAAAT CCGCCGCTTC ATCCCGCGCG ACTACTGGGA AGTGAAAGCC GAATTCGCGT GCGCGGGCGG CTTCTACGAA GGCAAGTGGT TCGACCCGAA ATTCAAGCGC GACGAATTCG ATCCGGAAAA GCGCGATTCC CGCCTGTGGA GCCTGCCGGC CGCCGAAACG ATCGTCGCCG CCTGCCGCGA CCAGGTCGGC ACGGTGTCCG AGGAATCGAA GCCGTCGACG CAGCTGTCGC CGCTGCTGTT CGACCTGACG AGCCTGCAGC GCGAGGCGAA CAGCCGCTTC GGCTTCTCCG CGAAGAATAC GCTCGGCCTC GCCCAGGCGC TGTATGAAAA GCACAAGGTG CTGACCTACC CGCGTACCGA CGCGCGCGCG CTGCCGGAAG ACTACCTGTC GACGGTCCAG TCCACGCTCG AGATGCTCAA GGAGAGCCAC AACTACCTGC CGCATGCCAA GCAGGTGCTC GACAAGGGCT GGGTGAAGCC GAACAAGCGG ATCTTCGACA ACTCGAAGAT CAGCGATCAC TTTGCCATCA TCCCGACGCT GCAGGCGCCG AAGTCGCTGT CCGAGCCGGA GCAGAAGCTG TACGACATGG TCGTGAAGCG CTTCCTCGCG GTGTTCTTCC CGGCCGCCGA ATTCCGCGTG ACGACGCGCA TCACCGAAGT CGCCGGGCAT CACTTCAAGA CCGAAGGCAA GGTGCTCGTC GAACCGGGCT GGCTGCAGGT GTACGGCCGC GACGCCGAAG GCGCGGACGC GAACCTCGTG CCGGTGCAGA AGGACGAGAA GGTGAAAACG GACGAAATCG CCGCCGTCGC GCTCGTGACC AAGCCGCCCG CACGCTACTC GGAAGCGACG CTGCTGTCCG CGATGGAAGG CGCCGGCAAG CTGGTCGAGG ACGACGAGTT GCGCGAGGCG ATGGCCGCGA AAGGTCTCGG CACGCCGGCG ACGCGCGCGG CGATCATCGA AGGCCTGCTC GGAGAGAAGT ACCTGCTGCG CGAGGGCCGC GAACTGATCC CGACCGCGAA GGCATTCCAG CTGATGACGC TGCTGCGCGG CCTCGGCGTG AAGGAGCTCA CCGCGCCCGA GCTCACCGGC GAATGGGAAT ACAAGCTGTC GCAGATGGAG CGCGGCAATC TCGGCCGCGA CGCGTTCATG CAGGAAATCG CCCGCATGAC GCAGCAGATC GTCAAGCGCG CGAAGGAATA CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA

Details for BuceA.18062.a.A3.PW33855

PURIFICATION DATe:	11/11/2011
CONCENTRATION:	34.17mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATTCCGAC ACGATTCCCG GCGACTACGC AACGCTCGAG ACGCCGTGCC CGAACTGCGG CGGCCAGGTG AAGGAAAACT ACCGCCGCTT CGCGTGCACG AAGTGCGACT TCTCGATCTC GAAGATCCCG GGCAGCCGGC AGTTCGAGAT TCCGGAAGTC GAGGAGCTGC TGCAGAAGAA GGAAATCGGG CCGCTGTCCG GGTTCCGCAG CAAGATGGGG CGCCCGTTCT CCGCGATCCT CAAGCTGTCC TTCGACGACG AGACGAAGAA CTACAAGCTC GAGTTCGATT TCGGCCAGGA CCAGGGCGGC GAGGAAGGCG AAGCGCCCGA CTTCTCCGCG CAGGAACCGG TCGGCGCGTG CCCGAAGTGC AAGGGCCGCG TGTTCGAGCA CGGGATGAGC TACGTGTGCG AGCACTCGGT CGCGAACCCG AAGACCTGCG ACTTCCGCTC GGGCAAGGTG ATCCTGCAAC AGGAAATCAC GCGCGAGCAG ATGGGCAAGC TGCTGGCCGA CGGCCGCACG GATCTGCTGC CGAACTTCAA GTCGTCGCGC ACCGGCCGCA ACTTCAAGGC GTTCCTCGTG AAGCAGCCGG ACGGCAAGAT CGGTTTCGAG TTCGAGAAGA AGGAGCCGAA AGCCGCCGCC GCGAAGAAGA CGGCCAAATC GGCGACGAAG GACGCCGAAA CCGTGACGGA AGGCGCCGAG GAGAAACCGG CACCGGCCCG CAAGACCGCC GCCCGCAAAA CCACGGCGCG CAAGACGGGT TCGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA