SSGCID: Target Page

BuceA.18056.a

Putative type VI secretion system protein TssC

CENTER ID:	BuceA.18056.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18056.a.A1.GE33240	full length		1	496

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.359
RefSeq:	YP_002229508.1
UniProt:	B4E6R4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNQQTAAAQA SGAEYAAGTS LLDDIVEKSK VAKSDSEHAR AKDLIGELVH QVLDGTVIVS DNLSATIDAR VAELDRLIST QLSAVMHAPE FQRLESTWRG LDYLVKESNT GQTIKIKALH APKRDLVRDF KGASEFDQSA LFKKVYEEEF GTFGGSPFGA LIGDYEISRQ PEDMYFIEQM SHVAAAAHAP FIASASPELL GLESFSDLGK PRDLGKVFDT VEYAKWKSFR DAEDSRYVGL TLPRFLGRLP FNPKDGQTAE NFNFVEDVDG TDHDKYLWCN AAWAFAARLT AAFDDFGWCA AIRGVEGGGL VEDLPTHTFK TDDGEVALKC PTEIAITDRR EKELSDLGFI PLVHCKNSDY AAFFAAQSVQ KPKKYSTDSA NANAVLSAQL QYIFSVSRVA HYLKAMMRDK IGSFASAQNV ETFLNRWISQ YVLLDDNASQ EQKAQFPLRE ASIQVSEIPG KPGSYRSVAF LRPHFQLDEL SISLRLVADL PKPANS

NT Sequence

atgaaccagc aaacggctgc ggcccaagcg agcggcgcgg aatacgccgc cgggacttcg ttgctcgacg acatcgtcga gaagagcaag gtcgcgaaat ccgattccga gcatgcgcgc gcgaaggacc tgatcggcga actcgtgcac caggtgctcg acggcacggt gatcgtgtcg gacaacctgt cggccacgat cgatgcgcgc gtcgcggaac tcgaccgcct gatttccacg cagctttccg cggtgatgca cgcgccggaa ttccagcgcc tcgaaagcac gtggcgcggg ctggactatc tggtcaagga aagcaacacg ggccagacga tcaagatcaa ggcgctgcac gcaccgaagc gcgacctcgt gcgcgatttc aagggcgcga gcgagttcga ccagagcgcg ctgttcaaga aggtctacga agaggaattc ggcacgttcg gcggttcgcc gttcggtgcg ctgatcggcg attacgagat ctcgcgccag ccggaagaca tgtatttcat cgagcagatg tcgcacgtcg cggcggccgc gcatgcgccg ttcatcgcgt cggcgtcgcc ggagctgctc ggcctcgagt cgttctccga cctcggcaag ccgcgcgacc tcggcaaggt gttcgacacc gtcgaatacg cgaagtggaa atcgttccgc gacgccgagg attcccgcta cgtcggcctg acgctgccgc gcttcctcgg ccgcctgccg ttcaatccga aggacggcca gaccgcggag aacttcaact tcgtcgagga cgtcgacggc accgaccacg acaagtacct gtggtgcaac gcggcatggg ccttcgcggc acgcctgacg gccgcgttcg acgacttcgg ctggtgcgcg gcgatccgcg gcgtcgaagg cggtggcctc gtcgaggatc tgccgaccca cacgttcaag accgacgacg gtgaagtcgc gctgaagtgc ccgaccgaaa tcgcgatcac cgatcgccgc gagaaggagc tgagcgacct cggcttcatc ccgctcgtcc attgcaagaa ctcagattac gccgcgttct tcgctgcgca gtcggtgcag aagccgaaaa agtacagcac cgacagcgcg aatgcgaacg ccgtcctgtc cgcccagctt cagtacatct tctcggtatc gcgcgtcgcg cactacctga aggcgatgat gcgggacaag atcggcagct tcgcatcggc gcagaacgtg gagactttcc tcaaccggtg gatttcgcag tacgtcctgc tcgacgacaa cgcctcgcag gaacagaaag cgcaattccc gctgcgcgag gcatccatac aagtgtcgga gattccgggc aagccgggct cgtatcgttc ggtcgcgttc ctgcgtccgc actttcagct cgacgaactc tcgatttctc tgcgacttgt cgctgatctg cccaaaccgg caaattcata a

Details for BuceA.18056.a.A1.GE33240

HARVESTED ON:	8/30/2011
SEQUENCED ON:	9/2/2011
EXPECTED MW:	57kDa
OBSERVED MW:	57kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	85
PERCENT COVERAGE:	55

Validated AA Sequence

Validated NT Sequence

ggntcnnnnn catnaccatc atangggtac cctggaagct cagacccagg gtcctggttc gatgaaccag caaacggctg cggcccaagc gagcggcgcg gaatacgccg ccgggacttc gttgctcgac gacatcgtcg agaagagcaa ggtcgcgaaa tccgattccg agcatgcgcg cgcgaaggac ctgatcggcg aactcgtgca ccaggtgctc gacggcacgg tgatcgtgtc ggacaacctg tcggccacga tcgatgcgcg cgtcgcggaa ctcgaccgcc tgatttccac gcagctttcc gcggtgatgc acgcgccgga attccagcgc ctcgaaagca cgtggcgcgg gctggactat ctggtcaagg aaagcaacac gggccagacg atcaagatca aggcgctgca cgcaccgaag cgcgacctcg tgcgcgattt caagggcgcg agcgagttcg accagagcgc gctgttcaag aaggtctacg aagaggaatt cggcacgttc ggcggttcgc cgttcggtgc gctgatcggc gattacgaga tctcgcgcca gccggaagac atgtatttca tcgagcagat gtcgcacgtc gcggcggccg cgcatgcgcc gttcatcgcg tcggcgtcgc cggagctgct cggcctcgag tcgttctccg acctcggcaa gccgcgcgac ctcggcaagg tgttcgacac cgtcgaatac gcgaagtgga aatcgttccg cgacgccnag gattcgctac gtcggcctga cgctgccgcg cttcctcggc cgcctgccgt tcaatccnaa nggacggnca gaccgcggag aacttcaact tcgtcgagga cgtcganggc accgaccacg acaagtacct gtggtgcaac gcngcatggg ccttcgcggc acgcctgacg gncgcgttcg acgantncng ctggngcgcn gcganngcgg ngtcnannng ntggnctcgt cnnngnnctg nnnacccncn cgntcanann gannangntn annnnnnctn annnncngac gaancnnnat atngcncnan nnnncnnnnn nnnnnnnnnt ccnnnnnncn tgnanannnn nnnnnnnnnn nnnnnnncnn nnnnnnnnnn nnnnnnnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNQQTAAAQ ASGAEYAAGT SLLDDIVEKS KVAKSDSEHA RAKDLIGELV HQVLDGTVIV SDNLSATIDA RVAELDRLIS TQLSAVMHAP EFQRLESTWR GLDYLVKESN TGQTIKIKAL HAPKRDLVRD FKGASEFDQS ALFKKVYEEE FGTFGGSPFG ALIGDYEISR QPEDMYFIEQ MSHVAAAAHA PFIASASPEL LGLESFSDLG KPRDLGKVFD TVEYAKWKSF RDAEDSRYVG LTLPRFLGRL PFNPKDGQTA ENFNFVEDVD GTDHDKYLWC NAAWAFAARL TAAFDDFGWC AAIRGVEGGG LVEDLPTHTF KTDDGEVALK CPTEIAITDR REKELSDLGF IPLVHCKNSD YAAFFAAQSV QKPKKYSTDS ANANAVLSAQ LQYIFSVSRV AHYLKAMMRD KIGSFASAQN VETFLNRWIS QYVLLDDNAS QEQKAQFPLR EASIQVSEIP GKPGSYRSVA FLRPHFQLDE LSISLRLVAD LPKPANS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaccagcaa acggctgcgg cccaagcgag cggcgcggaa tacgccgccg ggacttcgtt gctcgacgac atcgtcgaga agagcaaggt cgcgaaatcc gattccgagc atgcgcgcgc gaaggacctg atcggcgaac tcgtgcacca ggtgctcgac ggcacggtga tcgtgtcgga caacctgtcg gccacgatcg atgcgcgcgt cgcggaactc gaccgcctga tttccacgca gctttccgcg gtgatgcacg cgccggaatt ccagcgcctc gaaagcacgt ggcgcgggct ggactatctg gtcaaggaaa gcaacacggg ccagacgatc aagatcaagg cgctgcacgc accgaagcgc gacctcgtgc gcgatttcaa gggcgcgagc gagttcgacc agagcgcgct gttcaagaag gtctacgaag aggaattcgg cacgttcggc ggttcgccgt tcggtgcgct gatcggcgat tacgagatct cgcgccagcc ggaagacatg tatttcatcg agcagatgtc gcacgtcgcg gcggccgcgc atgcgccgtt catcgcgtcg gcgtcgccgg agctgctcgg cctcgagtcg ttctccgacc tcggcaagcc gcgcgacctc ggcaaggtgt tcgacaccgt cgaatacgcg aagtggaaat cgttccgcga cgccgaggat tcccgctacg tcggcctgac gctgccgcgc ttcctcggcc gcctgccgtt caatccgaag gacggccaga ccgcggagaa cttcaacttc gtcgaggacg tcgacggcac cgaccacgac aagtacctgt ggtgcaacgc ggcatgggcc ttcgcggcac gcctgacggc cgcgttcgac gacttcggct ggtgcgcggc gatccgcggc gtcgaaggcg gtggcctcgt cgaggatctg ccgacccaca cgttcaagac cgacgacggt gaagtcgcgc tgaagtgccc gaccgaaatc gcgatcaccg atcgccgcga gaaggagctg agcgacctcg gcttcatccc gctcgtccat tgcaagaact cagattacgc cgcgttcttc gctgcgcagt cggtgcagaa gccgaaaaag tacagcaccg acagcgcgaa tgcgaacgcc gtcctgtccg cccagcttca gtacatcttc tcggtatcgc gcgtcgcgca ctacctgaag gcgatgatgc gggacaagat cggcagcttc gcatcggcgc agaacgtgga gactttcctc aaccggtgga tttcgcagta cgtcctgctc gacgacaacg cctcgcagga acagaaagcg caattcccgc tgcgcgaggc atccatacaa gtgtcggaga ttccgggcaa gccgggctcg tatcgttcgg tcgcgttcct gcgtccgcac tttcagctcg acgaactctc gatttctctg cgacttgtcg ctgatctgcc caaaccggca aattcataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa