SSGCID: Target Page

BuceA.18055.a

DNA-directed RNA polymerase subunit beta (RNAP subunit beta) (EC 2.7.7.6) (RNA polymerase subunit beta) (Transcriptase subunit beta)

CENTER ID:	BuceA.18055.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18055.a.A2.GE33238	domain 1		1	722
BuceA.18055.a.A3.GE33239	domain 2		723	1368

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18055.a.A2.PS01298	domain 1		1	722

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.236
RefSeq:	YP_002229393.1
UniProt:	B4E5B2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQYSFTEKKR IRKSFAKRPI VHQVPFLLAT QLESFSTFLQ ADVPATQRKP EGLQAAFTSV FPIVSHNGFA RLEFVSYALS SPAFNIKECQ QRGLTYCSAL RAKVRLVILD KESPNKPVVK EVKEQEVYMG EIPLMTPTGS FVINGTERVI VSQLHRSPGV FFEHDKGKTH SSGKLLFSAR IIPYRGSWLD FEFDPKDILY FRVDRRRKMP VTILLKAIGL TPEQILANFF VFDNFTLMDE GAQLEFVPER LRGEVARFDI TDRDGKVIVQ KDKRINAKHI RDLEAAKTKF ISVPEDYLLG RVLAKNVVDG DTGEVIASAN DEVTESVLEK LREAGIKDIQ TLYTNDLDQG PYISSTLRVD ETTDKTAARI AIHRMMRPGE PPTEEAVEAL FNRLFYSEEA YDLSKVGRMK FNRRVGRDEI VGPMTLQDDD ILATIKILVE LRNGKGEVDD IDHLGNRRVR CVGELAENQF RAGLVRVERA VKERLGQAES ENLMPHDLIN SKPISSAIRE FFGSSQLSQF MDQTNPLSEI THKRRVSALG PGGLTRERAG FEVRDVHPTH YGRVCPIETP EGPNIGLINS LALYAHLNEY GFLETPYRKV VDSKVTDQID YLSAIEEGRY MIAQANAAID EDGRLIDELV SSREAGETMM VTPDRIQYMD VAPSQIVSVA ASLIPFLEHD DANRALMGSN MQRQAVPCLR PEKPVVGTGI ERTCAVDSGT TVQAFRGGVV DYVDAGRIVI RVNDDEAVAG EVGVDIYNLI KYTRSNQNTN INQRPIVKMG DKVSRGDVLA DGASTDLGEL ALGQNMLIAF MPWNGYNFED SILISEKVVA DDRYTSIHIE ELNVVARDTK LGPEEITRDI SNLAEVQLGR LDESGIVYIG AEVEAGDVLV GKVTPKGETQ LTPEEKLLRA IFGEKASDVK DTSLRVPSGM SGTVIDVQVF TREGIQRDKR AQQIIDDELK RYRLDLNDQL RIVEGDAFQR LARMLVGKVA NGGPKKLAKG TKIDQAYLED LDHYHWFDIR LADDEAAASL EAIKNSIEEK RHQFDLAFEE KRKKLTQGDE LPPGVLKMVK VYLAVKRRLQ PGDKMAGRHG NKGVVSKIVP IEDMPYMADG RPADVVLNPL GVPSRMNVGQ VLEVHLGWAA KGLGWRIGEM LQRQAKIEEL RTFLTKIYNE SGRQEDLESF TDDEILELAK NLREGVPFAT PVFDGATEEE MGKMLDLAFP DDIAEQLGMN PSKNQVRLYD GRTGEMFERR VTLGYMHYLK LHHLVDDKMH ARSTGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAS YVLQEMLTVK SDDVTGRTKV YENLVKGDHV IDAGMPESFN VLVKEIRSLG IDIDLDRN

NT Sequence

atgcaatatt ccttcaccga gaagaagcgc attcgcaaga gtttcgcgaa gcgccccatc gttcaccaag ttccgttctt gctggctact cagcttgaat cattcagcac gtttctgcaa gccgatgtgc ccgcgacgca acgtaagccc gagggtttgc aggccgcgtt cacgtcggta tttcccattg tttcgcacaa cggtttcgcg cgcctcgagt tcgtgagcta tgcgctgtcc tcgccggcat tcaacatcaa ggaatgccag cagcgaggcc tgacgtactg ctccgcgctg cgcgcgaagg tccgcctcgt catcctcgac aaggaatcgc cgaacaagcc ggtcgtcaag gaagtgaagg agcaggaagt gtacatgggc gaaattccgc tcatgacgcc gacgggctcg ttcgtcatca acggcaccga gcgtgtcatc gtctcgcagc tgcatcgttc gcctggcgtg ttcttcgaac acgacaaggg caagacgcac agctcgggca agctgctgtt ctcggcacgg atcattccgt accgcggttc gtggctcgac ttcgaattcg atccgaagga catcctgtac ttccgcgtcg accgtcgccg caagatgccg gtcacgatcc tgctgaaggc catcggcctg acgccggaac agatcctcgc gaacttcttc gtgttcgaca atttcacgct gatggacgaa ggcgcgcaac tcgagttcgt gcccgagcgc ctgcgtggtg aagtcgcgcg tttcgacatc acggatcgtg atggcaaggt catcgtccag aaggacaagc ggatcaacgc gaagcacatt cgcgacctcg aagccgcgaa gaccaagttc atctcggtgc cggaagacta tctgctcggc cgcgtgctgg cgaagaacgt cgtcgacggc gacaccggcg aagtgatcgc gagcgcgaac gacgaagtca cggaaagcgt gctcgagaag ctgcgcgaag cgggcatcaa ggacatccag acgctctaca cgaacgatct ggatcagggc ccgtacatct cgtcgacgct gcgtgtcgac gaaacgaccg acaagacggc cgcgcgcatc gcgatccacc gcatgatgcg tccgggcgag ccgccgaccg aagaagcggt cgaggccctg ttcaaccgtc tgttctacag cgaagaagcg tacgacctgt cgaaggtcgg ccgcatgaag ttcaaccgcc gcgtcggccg tgacgagatc gtcggcccga tgacgctgca ggacgacgac atcctcgcga cgatcaagat cctcgtcgag ctgcgcaacg gcaagggcga agtggacgac atcgaccacc tcggcaaccg tcgcgtgcgt tgcgtcggcg aactggcgga aaaccagttc cgcgccggtc tcgtgcgtgt cgagcgcgcg gtcaaggaac gcctcggcca ggccgaaagc gaaaacctga tgccgcacga cctgatcaac tcgaagccga tttcgtcggc gattcgcgag ttcttcggct cgtcgcagct gtcgcagttc atggaccaga ccaacccgct gtcggaaatc acgcacaagc gccgtgtttc cgcactgggc ccgggcggtc tgacgcgcga acgcgcaggc ttcgaagtcc gcgacgtgca cccgacccac tacggccgcg tgtgcccgat cgaaacgccg gaaggtccga acatcggcct gatcaactcg ctcgcgctgt acgcgcacct gaacgagtac ggcttcctcg agacgccgta ccgcaaggtc gtggacagca aggtgaccga tcagatcgac tacctgtcgg cgatcgagga aggccgctac atgatcgctc aggcgaacgc cgcgatcgac gaagacggcc gactcatcga cgaactcgtg tcgtcgcgcg aagccggcga aacgatgatg gtcacgccgg accgtatcca gtacatggac gtcgcgccgt cgcagatcgt gtcggtggcc gcatcgctga ttccgttcct cgagcacgat gacgcgaacc gcgcactgat gggctcgaac atgcagcgtc aggccgtgcc gtgtctgcgt ccggaaaagc cggtcgtcgg tacgggcatc gagcgtacct gcgcggtcga ctccggtacg acggtccagg cgttccgcgg cggtgtggtc gactacgtcg acgcaggccg tatcgtgatt cgcgtgaacg acgacgaagc agtcgcgggt gaagtcggtg tcgacatcta caacctgatc aagtacacgc gttcgaacca gaacacgaac atcaaccagc gtccgatcgt gaagatgggc gacaaggtct cgcgcggcga cgtgctggcc gacggcgcct cgacggacct gggcgagctc gcgctcggcc agaacatgct gatcgcgttc atgccgtgga acggctacaa cttcgaggat tcgatcctga tctcggagaa agtggtcgcg gacgatcgct acacgtcgat ccacatcgaa gagctgaacg tcgttgcacg cgacacgaag ctcgggccgg aagaaatcac gcgcgacatc tcgaacctgg cggaagtcca gctcggccgt ctcgacgaat cgggcatcgt gtacatcggt gctgaagtcg aagcaggcga cgtgctggtc ggcaaggtca cgccgaaggg cgagacccag ctgacgccgg aagagaagct gctgcgcgcg atcttcggcg agaaggcttc ggacgtgaag gacacgtcgc tgcgcgtgcc gtcgggcatg agcggcaccg tgatcgacgt ccaggtgttc acgcgtgaag gcatccagcg cgacaagcgt gcgcaacaga tcatcgacga cgaactgaag cgctaccgtc tcgacctgaa cgaccagctg cgcatcgtgg aaggcgacgc gttccagcgt ctcgcccgca tgctcgtggg caaggtcgcg aacggcggtc cgaagaagct cgcgaagggc acgaagatcg accaggctta cctggaagat ctcgaccact accactggtt cgacatccgc ctcgcggacg acgaagcagc ggcatcgctc gaagcgatca agaactcgat cgaagagaag cgtcaccagt tcgatctcgc gttcgaagaa aagcgcaaga agctcacgca aggcgacgaa ctgccgccgg gcgtgctgaa gatggtcaag gtgtacctcg cggtgaagcg ccgtctgcag cctggcgaca agatggcagg ccgtcacggt aacaagggtg tcgtgtcgaa gatcgtcccg atcgaagaca tgccgtacat ggccgatggc cgtccggcag acgtcgtgct gaacccgctc ggcgtgccgt cgcggatgaa cgtgggtcag gttctggaag tgcacctcgg ctgggccgcg aagggcctcg gctggcgtat cggcgaaatg ctgcagcgtc aggcgaagat cgaggaactg cgcacgttcc tgacgaagat ctacaacgag tcgggccgcc aggaagatct ggaaagcttc accgacgacg agatcctcga actcgcgaag aacctgcgcg aaggcgtgcc gttcgcgacg ccggtgttcg acggtgcgac cgaggaagaa atgggcaaga tgctcgacct cgcgttcccg gacgacatcg ccgaacagct cggcatgaac ccgtcgaaga accaggtccg tctgtacgac ggccgcacgg gtgaaatgtt cgaacgtcgc gtgacgctcg gctacatgca ctacctgaag ctgcaccact tggtcgacga caagatgcac gcgcgttcga ccggcccgta ctcgctcgtc acgcagcagc cgctgggtgg taaggcgcag ttcggtggcc agcgtttcgg tgaaatggaa gtgtgggcac tcgaagcgta cggcgcgtcc tacgtgctgc aggaaatgct gacggtgaag tcggacgacg tgaccggccg gacgaaggtg tacgaaaacc tcgtcaaggg cgaccacgtg atcgatgcag gcatgccgga atccttcaac gtgctcgtga aggaaatccg ctcgctcggt atcgatatcg atctcgaccg caattaa

Details for BuceA.18055.a.A2.GE33238

HARVESTED ON:	8/30/2011
SEQUENCED ON:	9/2/2011
EXPECTED MW:	83kDa
OBSERVED MW:	82kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	75
PERCENT COVERAGE:	54

Validated AA Sequence

Validated NT Sequence

tntnnnnnnn nnagnnatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgcaa tattccttca ccgagaagaa gcgcattcgc aagagtttcg cgaagcgccc catcgttcac caagttccgt tcttgctggc tactcagctt gaatcattca gcacgtttct gcaagccgat gtgcccgcga cgcaacgtaa gcccgagggt ttgcaggccg cgttcacgtc ggtatttccc attgtttcgc acaacggttt cgcgcgcctc gagttcgtga gctatgcgct gtcctcgccg gcattcaaca tcaaggaatg ccagcagcga ggcctgacgt actgctccgc gctgcgcgcg aaggtccgcc tcgtcatcct cgacaaggaa tcgccgaaca agccggtcgt caaggaagtg aaggagcagg aagtgtacat gggcgaaatt ccgctcatga cgccgacggg ctcgttcgtc atcaacggca ccgagcgtgt catcgtctcg cagctgcatc gttcgcctgg cgtgttcttc gaacacgaca agggcaagac gcacagctcg ggcaagctgc tgttctcggc acggatcatt ccgtaccgcg gttcgtggct cgacttcgaa ttcgatccga aggacatcct gtacttccgc gtcgaccgtc gccgcaagat gccggtcacg atcctgctga aggccatcgg cctgacgccg gaacagatcc tcgcgaactt cttcgtgttc gacaatttca cgctgatgga cgannncgcg caactcgagt tcgtgcccga ncgcctgcgt ggtgaagtcg cgcgtttcga catcacggat cgtgatgnca aggtcatcgt ccagaangga caagcggatc aacgcgaanc anattcgcga cctcnaagcc gcnaagaccn agttcatctc ggtgccngaa nnactatctg ctcgccgcgt gctggncnaa naangtcgtc nannncnann ccngcnaann nntcgcgagc nnnaacgacn aanncncnna annnntgnnn nnnnanctgc nannnnggnn atnnnnnnnn ncnnnnnnnn nnntacnnnn angancnnga ncnnnnnnnn nnntnntnnn nnnnannnnn ncnnnnnnnn naannnnnaa nnannnnnnn nnnnnnnnnn nnntnnnnnn nnnnnnnnnn nnnnnnana

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMQYSFTEKK RIRKSFAKRP IVHQVPFLLA TQLESFSTFL QADVPATQRK PEGLQAAFTS VFPIVSHNGF ARLEFVSYAL SSPAFNIKEC QQRGLTYCSA LRAKVRLVIL DKESPNKPVV KEVKEQEVYM GEIPLMTPTG SFVINGTERV IVSQLHRSPG VFFEHDKGKT HSSGKLLFSA RIIPYRGSWL DFEFDPKDIL YFRVDRRRKM PVTILLKAIG LTPEQILANF FVFDNFTLMD EGAQLEFVPE RLRGEVARFD ITDRDGKVIV QKDKRINAKH IRDLEAAKTK FISVPEDYLL GRVLAKNVVD GDTGEVIASA NDEVTESVLE KLREAGIKDI QTLYTNDLDQ GPYISSTLRV DETTDKTAAR IAIHRMMRPG EPPTEEAVEA LFNRLFYSEE AYDLSKVGRM KFNRRVGRDE IVGPMTLQDD DILATIKILV ELRNGKGEVD DIDHLGNRRV RCVGELAENQ FRAGLVRVER AVKERLGQAE SENLMPHDLI NSKPISSAIR EFFGSSQLSQ FMDQTNPLSE ITHKRRVSAL GPGGLTRERA GFEVRDVHPT HYGRVCPIET PEGPNIGLIN SLALYAHLNE YGFLETPYRK VVDSKVTDQI DYLSAIEEGR YMIAQANAAI DEDGRLIDEL VSSREAGETM MVTPDRIQYM DVAPSQIVSV AASLIPFLEH DDANRALMGS NMQRQAVPCL RPEKPVVGTG IERTCAVDSG TTV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaatattcc ttcaccgaga agaagcgcat tcgcaagagt ttcgcgaagc gccccatcgt tcaccaagtt ccgttcttgc tggctactca gcttgaatca ttcagcacgt ttctgcaagc cgatgtgccc gcgacgcaac gtaagcccga gggtttgcag gccgcgttca cgtcggtatt tcccattgtt tcgcacaacg gtttcgcgcg cctcgagttc gtgagctatg cgctgtcctc gccggcattc aacatcaagg aatgccagca gcgaggcctg acgtactgct ccgcgctgcg cgcgaaggtc cgcctcgtca tcctcgacaa ggaatcgccg aacaagccgg tcgtcaagga agtgaaggag caggaagtgt acatgggcga aattccgctc atgacgccga cgggctcgtt cgtcatcaac ggcaccgagc gtgtcatcgt ctcgcagctg catcgttcgc ctggcgtgtt cttcgaacac gacaagggca agacgcacag ctcgggcaag ctgctgttct cggcacggat cattccgtac cgcggttcgt ggctcgactt cgaattcgat ccgaaggaca tcctgtactt ccgcgtcgac cgtcgccgca agatgccggt cacgatcctg ctgaaggcca tcggcctgac gccggaacag atcctcgcga acttcttcgt gttcgacaat ttcacgctga tggacgaagg cgcgcaactc gagttcgtgc ccgagcgcct gcgtggtgaa gtcgcgcgtt tcgacatcac ggatcgtgat ggcaaggtca tcgtccagaa ggacaagcgg atcaacgcga agcacattcg cgacctcgaa gccgcgaaga ccaagttcat ctcggtgccg gaagactatc tgctcggccg cgtgctggcg aagaacgtcg tcgacggcga caccggcgaa gtgatcgcga gcgcgaacga cgaagtcacg gaaagcgtgc tcgagaagct gcgcgaagcg ggcatcaagg acatccagac gctctacacg aacgatctgg atcagggccc gtacatctcg tcgacgctgc gtgtcgacga aacgaccgac aagacggccg cgcgcatcgc gatccaccgc atgatgcgtc cgggcgagcc gccgaccgaa gaagcggtcg aggccctgtt caaccgtctg ttctacagcg aagaagcgta cgacctgtcg aaggtcggcc gcatgaagtt caaccgccgc gtcggccgtg acgagatcgt cggcccgatg acgctgcagg acgacgacat cctcgcgacg atcaagatcc tcgtcgagct gcgcaacggc aagggcgaag tggacgacat cgaccacctc ggcaaccgtc gcgtgcgttg cgtcggcgaa ctggcggaaa accagttccg cgccggtctc gtgcgtgtcg agcgcgcggt caaggaacgc ctcggccagg ccgaaagcga aaacctgatg ccgcacgacc tgatcaactc gaagccgatt tcgtcggcga ttcgcgagtt cttcggctcg tcgcagctgt cgcagttcat ggaccagacc aacccgctgt cggaaatcac gcacaagcgc cgtgtttccg cactgggccc gggcggtctg acgcgcgaac gcgcaggctt cgaagtccgc gacgtgcacc cgacccacta cggccgcgtg tgcccgatcg aaacgccgga aggtccgaac atcggcctga tcaactcgct cgcgctgtac gcgcacctga acgagtacgg cttcctcgag acgccgtacc gcaaggtcgt ggacagcaag gtgaccgatc agatcgacta cctgtcggcg atcgaggaag gccgctacat gatcgctcag gcgaacgccg cgatcgacga agacggccga ctcatcgacg aactcgtgtc gtcgcgcgaa gccggcgaaa cgatgatggt cacgccggac cgtatccagt acatggacgt cgcgccgtcg cagatcgtgt cggtggccgc atcgctgatt ccgttcctcg agcacgatga cgcgaaccgc gcactgatgg gctcgaacat gcagcgtcag gccgtgccgt gtctgcgtcc ggaaaagccg gtcgtcggta cgggcatcga gcgtacctgc gcggtcgact ccggtacgac ggtctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BuceA.18055.a.A3.GE33239

HARVESTED ON:	8/30/2011
SEQUENCED ON:	9/2/2011
EXPECTED MW:	74kDa
OBSERVED MW:	74kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	73
PERCENT COVERAGE:	58

Validated AA Sequence

Validated NT Sequence

ttnnnnangg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcaggc gttccgcggc ggtgtggtcg actacgtcga cgcaggccgt atcgtgattc gcgtgaacga cgacgaagca gtcgcgggtg aagtcggtgt cgacatctac aacctgatca agtacacgcg ttcgaaccag aacacgaaca tcaaccagcg tccgatcgtg aagatgggcg acaaggtctc gcgcggcgac gtgctggccg acggcgcctc gacggacctg ggcgagctcg cgctcggcca gaacatgctg atcgcgttca tgccgtggaa cggctacaac ttcgaggatt cgatcctgat ctcggagaaa gtggtcgcgg acgatcgcta cacgtcgatc cacatcgaag agctgaacgt cgttgcacgc gacacgaagc tcgggccgga agaaatcacg cgcgacatct cgaacctggc ggaagtccag ctcggccgtc tcgacgaatc gggcatcgtg tacatcggtg ctgaagtcga agcaggcgac gtgctggtcg gcaaggtcac gccgaagggc gagacccagc tgacgccgga agagaagctg ctgcgcgcga tcttcggcga gaaggcttcg gacgtgaagg acacgtcgct gcgcgtgccg tcgggcatga gcggcaccgt gatcgacgtc caggtgttca cgcgtgaagg catccagcgc gacaagcgtg cgcaacagat catcgacgac gaactgaagc gctaccgtct cgacctgaac gaccagctgc gcatcgtggn aangngacgc gttccagcgt ctcgcccgca tgctcgtggg caaggtcgcg aacggcggtc cgaagaanct cgcgaagggc acgaanatcg accaggctta cctgggaaga tctcgaccac taccactggn ncgacatccg cctcgcggan gangaancag cggcatcgct cgaancgatc aannactcga tcgannanaa ncgtcnccng ttcgatcncn nnnnnnnnan nnnnnnnnac nnncnnnnnn gacnannnnn nngnnnnnnn nanatggnna gnnnnncntn nnnnnnnann nnnnnnnncn nnnnnnnnnn nnnnnnnnnn nncnnnnnan nnnnnnnnnn nnnnaannnn nnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMQAFRGGVV DYVDAGRIVI RVNDDEAVAG EVGVDIYNLI KYTRSNQNTN INQRPIVKMG DKVSRGDVLA DGASTDLGEL ALGQNMLIAF MPWNGYNFED SILISEKVVA DDRYTSIHIE ELNVVARDTK LGPEEITRDI SNLAEVQLGR LDESGIVYIG AEVEAGDVLV GKVTPKGETQ LTPEEKLLRA IFGEKASDVK DTSLRVPSGM SGTVIDVQVF TREGIQRDKR AQQIIDDELK RYRLDLNDQL RIVEGDAFQR LARMLVGKVA NGGPKKLAKG TKIDQAYLED LDHYHWFDIR LADDEAAASL EAIKNSIEEK RHQFDLAFEE KRKKLTQGDE LPPGVLKMVK VYLAVKRRLQ PGDKMAGRHG NKGVVSKIVP IEDMPYMADG RPADVVLNPL GVPSRMNVGQ VLEVHLGWAA KGLGWRIGEM LQRQAKIEEL RTFLTKIYNE SGRQEDLESF TDDEILELAK NLREGVPFAT PVFDGATEEE MGKMLDLAFP DDIAEQLGMN PSKNQVRLYD GRTGEMFERR VTLGYMHYLK LHHLVDDKMH ARSTGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAS YVLQEMLTVK SDDVTGRTKV YENLVKGDHV IDAGMPESFN VLVKEIRSLG IDIDLDRN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaggcgttc cgcggcggtg tggtcgacta cgtcgacgca ggccgtatcg tgattcgcgt gaacgacgac gaagcagtcg cgggtgaagt cggtgtcgac atctacaacc tgatcaagta cacgcgttcg aaccagaaca cgaacatcaa ccagcgtccg atcgtgaaga tgggcgacaa ggtctcgcgc ggcgacgtgc tggccgacgg cgcctcgacg gacctgggcg agctcgcgct cggccagaac atgctgatcg cgttcatgcc gtggaacggc tacaacttcg aggattcgat cctgatctcg gagaaagtgg tcgcggacga tcgctacacg tcgatccaca tcgaagagct gaacgtcgtt gcacgcgaca cgaagctcgg gccggaagaa atcacgcgcg acatctcgaa cctggcggaa gtccagctcg gccgtctcga cgaatcgggc atcgtgtaca tcggtgctga agtcgaagca ggcgacgtgc tggtcggcaa ggtcacgccg aagggcgaga cccagctgac gccggaagag aagctgctgc gcgcgatctt cggcgagaag gcttcggacg tgaaggacac gtcgctgcgc gtgccgtcgg gcatgagcgg caccgtgatc gacgtccagg tgttcacgcg tgaaggcatc cagcgcgaca agcgtgcgca acagatcatc gacgacgaac tgaagcgcta ccgtctcgac ctgaacgacc agctgcgcat cgtggaaggc gacgcgttcc agcgtctcgc ccgcatgctc gtgggcaagg tcgcgaacgg cggtccgaag aagctcgcga agggcacgaa gatcgaccag gcttacctgg aagatctcga ccactaccac tggttcgaca tccgcctcgc ggacgacgaa gcagcggcat cgctcgaagc gatcaagaac tcgatcgaag agaagcgtca ccagttcgat ctcgcgttcg aagaaaagcg caagaagctc acgcaaggcg acgaactgcc gccgggcgtg ctgaagatgg tcaaggtgta cctcgcggtg aagcgccgtc tgcagcctgg cgacaagatg gcaggccgtc acggtaacaa gggtgtcgtg tcgaagatcg tcccgatcga agacatgccg tacatggccg atggccgtcc ggcagacgtc gtgctgaacc cgctcggcgt gccgtcgcgg atgaacgtgg gtcaggttct ggaagtgcac ctcggctggg ccgcgaaggg cctcggctgg cgtatcggcg aaatgctgca gcgtcaggcg aagatcgagg aactgcgcac gttcctgacg aagatctaca acgagtcggg ccgccaggaa gatctggaaa gcttcaccga cgacgagatc ctcgaactcg cgaagaacct gcgcgaaggc gtgccgttcg cgacgccggt gttcgacggt gcgaccgagg aagaaatggg caagatgctc gacctcgcgt tcccggacga catcgccgaa cagctcggca tgaacccgtc gaagaaccag gtccgtctgt acgacggccg cacgggtgaa atgttcgaac gtcgcgtgac gctcggctac atgcactacc tgaagctgca ccacttggtc gacgacaaga tgcacgcgcg ttcgaccggc ccgtactcgc tcgtcacgca gcagccgctg ggtggtaagg cgcagttcgg tggccagcgt ttcggtgaaa tggaagtgtg ggcactcgaa gcgtacggcg cgtcctacgt gctgcaggaa atgctgacgg tgaagtcgga cgacgtgacc ggccggacga aggtgtacga aaacctcgtc aagggcgacc acgtgatcga tgcaggcatg ccggaatcct tcaacgtgct cgtgaaggaa atccgctcgc tcggtatcga tatcgatctc gaccgcaatt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for BuceA.18055.a.A2.PS01298

PURIFICATION DATe:	12/6/2011
CONCENTRATION:	67.6mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	75
PERCENT COVERAGE:	54

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAATATTCC TTCACCGAGA AGAAGCGCAT TCGCAAGAGT TTCGCGAAGC GCCCCATCGT TCACCAAGTT CCGTTCTTGC TGGCTACTCA GCTTGAATCA TTCAGCACGT TTCTGCAAGC CGATGTGCCC GCGACGCAAC GTAAGCCCGA GGGTTTGCAG GCCGCGTTCA CGTCGGTATT TCCCATTGTT TCGCACAACG GTTTCGCGCG CCTCGAGTTC GTGAGCTATG CGCTGTCCTC GCCGGCATTC AACATCAAGG AATGCCAGCA GCGAGGCCTG ACGTACTGCT CCGCGCTGCG CGCGAAGGTC CGCCTCGTCA TCCTCGACAA GGAATCGCCG AACAAGCCGG TCGTCAAGGA AGTGAAGGAG CAGGAAGTGT ACATGGGCGA AATTCCGCTC ATGACGCCGA CGGGCTCGTT CGTCATCAAC GGCACCGAGC GTGTCATCGT CTCGCAGCTG CATCGTTCGC CTGGCGTGTT CTTCGAACAC GACAAGGGCA AGACGCACAG CTCGGGCAAG CTGCTGTTCT CGGCACGGAT CATTCCGTAC CGCGGTTCGT GGCTCGACTT CGAATTCGAT CCGAAGGACA TCCTGTACTT CCGCGTCGAC CGTCGCCGCA AGATGCCGGT CACGATCCTG CTGAAGGCCA TCGGCCTGAC GCCGGAACAG ATCCTCGCGA ACTTCTTCGT GTTCGACAAT TTCACGCTGA TGGACGAAGG CGCGCAACTC GAGTTCGTGC CCGAGCGCCT GCGTGGTGAA GTCGCGCGTT TCGACATCAC GGATCGTGAT GGCAAGGTCA TCGTCCAGAA GGACAAGCGG ATCAACGCGA AGCACATTCG CGACCTCGAA GCCGCGAAGA CCAAGTTCAT CTCGGTGCCG GAAGACTATC TGCTCGGCCG CGTGCTGGCG AAGAACGTCG TCGACGGCGA CACCGGCGAA GTGATCGCGA GCGCGAACGA CGAAGTCACG GAAAGCGTGC TCGAGAAGCT GCGCGAAGCG GGCATCAAGG ACATCCAGAC GCTCTACACG AACGATCTGG ATCAGGGCCC GTACATCTCG TCGACGCTGC GTGTCGACGA AACGACCGAC AAGACGGCCG CGCGCATCGC GATCCACCGC ATGATGCGTC CGGGCGAGCC GCCGACCGAA GAAGCGGTCG AGGCCCTGTT CAACCGTCTG TTCTACAGCG AAGAAGCGTA CGACCTGTCG AAGGTCGGCC GCATGAAGTT CAACCGCCGC GTCGGCCGTG ACGAGATCGT CGGCCCGATG ACGCTGCAGG ACGACGACAT CCTCGCGACG ATCAAGATCC TCGTCGAGCT GCGCAACGGC AAGGGCGAAG TGGACGACAT CGACCACCTC GGCAACCGTC GCGTGCGTTG CGTCGGCGAA CTGGCGGAAA ACCAGTTCCG CGCCGGTCTC GTGCGTGTCG AGCGCGCGGT CAAGGAACGC CTCGGCCAGG CCGAAAGCGA AAACCTGATG CCGCACGACC TGATCAACTC GAAGCCGATT TCGTCGGCGA TTCGCGAGTT CTTCGGCTCG TCGCAGCTGT CGCAGTTCAT GGACCAGACC AACCCGCTGT CGGAAATCAC GCACAAGCGC CGTGTTTCCG CACTGGGCCC GGGCGGTCTG ACGCGCGAAC GCGCAGGCTT CGAAGTCCGC GACGTGCACC CGACCCACTA CGGCCGCGTG TGCCCGATCG AAACGCCGGA AGGTCCGAAC ATCGGCCTGA TCAACTCGCT CGCGCTGTAC GCGCACCTGA ACGAGTACGG CTTCCTCGAG ACGCCGTACC GCAAGGTCGT GGACAGCAAG GTGACCGATC AGATCGACTA CCTGTCGGCG ATCGAGGAAG GCCGCTACAT GATCGCTCAG GCGAACGCCG CGATCGACGA AGACGGCCGA CTCATCGACG AACTCGTGTC GTCGCGCGAA GCCGGCGAAA CGATGATGGT CACGCCGGAC CGTATCCAGT ACATGGACGT CGCGCCGTCG CAGATCGTGT CGGTGGCCGC ATCGCTGATT CCGTTCCTCG AGCACGATGA CGCGAACCGC GCACTGATGG GCTCGAACAT GCAGCGTCAG GCCGTGCCGT GTCTGCGTCC GGAAAAGCCG GTCGTCGGTA CGGGCATCGA GCGTACCTGC GCGGTCGACT CCGGTACGAC GGTCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA