BuceA.18054.a

Putative lipoprotein

CENTER ID: BuceA.18054.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18054.a.A1.GE33237 full length 1 321
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2505
RefSeq: YP_002231522.1
UniProt: B4E6C4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGGLPFPAS RKSLPSSTVF LILAAAALLS GCGLLPVQNP PAPISEALVE SVEETAGEPL TMPPVPAPTQ PETPEAPKKP HREVTRSKPV QRPESPTPPP PPPPPIVATR PLDRTQIHAL LDSEVARRNG KVVGRAVDMV VDASGKPREM IVNLQGFMGI GDRKVIFPWN VFRFTPGGKQ EPIVLDVPSG DLPPAARPKA VPLSGSAAAS PTTRLPMLDS DVERPNGTKI GRIVDVLIDR GAQPQAVVLD LGGLVNTDRR SIAASWGALR FVTRDKALHP QLDLNDAQIK ASPPYAADKP IVAVFPPVVP AAPASSASAT R
NT Sequence
atgagcggcg gactcccgtt tcccgcatcc cgcaagtcgt tgccgtcgtc gacggtcttc ctgattctcg ccgccgccgc gctgctgtcc ggctgcgggc tgctgccggt ccagaacccg cccgccccga tcagcgaggc gctcgtcgag tcggtcgagg agaccgccgg cgagccgctg acgatgccgc ccgtgccggc gccgacgcaa ccggaaacgc cggaagcgcc gaagaagccg caccgcgagg tgacgcggtc gaaacccgtg cagcgccccg aatcgccgac cccgccaccc cctccgcccc cgccgatcgt cgcgacgcgc ccgctcgacc gtacccagat ccacgcgctg ctcgacagcg aagtcgcgcg ccgcaacggc aaggtggtcg gccgcgcggt cgacatggtg gtcgacgcga gcggcaagcc gcgcgagatg atcgtcaacc tgcaaggctt catgggcatc ggcgaccgca aggtcatctt cccgtggaac gtgttccgct tcacgccggg cggcaagcag gagccgatcg tgctcgacgt cccgtcgggc gacctgccgc cggccgcgcg accgaaggcg gtgccgctgt cgggttcggc cgcggcgtcg ccgacgacgc ggctgccgat gctcgacagc gatgtcgagc gcccgaacgg cacgaagatc ggccgcatcg tcgacgtgct gatcgaccgc ggtgcacagc cgcaggccgt cgtgctcgac ctcggcgggc tcgtcaatac cgaccgccgt tcgatcgccg cgagctgggg cgcgctgcgc ttcgtcacgc gcgacaaggc gctgcacccg cagctcgacc tgaacgacgc ccagatcaag gcatcgccgc cctacgcggc cgacaagccg atcgtcgcgg tcttcccgcc cgttgtcccg gccgcgccgg cttcgtctgc gagtgccacc cgatga
Details for BuceA.18054.a.A1.GE33237
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 36kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 88
PERCENT COVERAGE: 86
Validated AA Sequence
MGTLEAQTQG PGSMSGGLPF PASRKSLPSS TVFLILAAAA LLSGCGLLPV QNPPAPISEA LVESVEETAG EPLTMPPVPA PTQPETPEAP KKPHREVTRS KPVQRPESPT PPPPPPPPIV ATRPLDRTQI HALLDSEVAR RNGKVVGRAV DMVVDASGKP REMIVNLQGF MGIGDRKVIF PWNVFRFTPG GKXEPIVLDV PSGDLPPAAR PKAVPLSGSA AASPTTRXAD ARQRCXAPER HXDRPHXXVL IDRGAQPQAV VLDLXGLVXT DRRSIAASWG XAALRXARQX RCXRXSX
Validated NT Sequence
gntncannnc nnnncccntc atatgggtac cctggaagct cagacccagg gtcctggttc gatgagcggc ggactcccgt ttcccgcatc ccgcaagtcg ttgccgtcgt cgacggtctt cctgattctc gccgccgccg cgctgctgtc cggctgcggg ctgctgccgg tccagaaccc gcccgccccg atcagcgagg cgctcgtcga gtcggtcgag gagaccgccg gcgagccgct gacgatgccg cccgtgccgg cgccgacgca accggaaacg ccggaagcgc cgaagaagcc gcaccgcgag gtgacgcggt cgaaacccgt gcagcgcccc gaatcgccga ccccgccacc ccctccgccc ccgccgatcg tcgcgacgcg cccgctcgac cgtacccaga tccacgcgct gctcgacagc gaagtcgcgc gccgcaacgg caaggtggtc ggccgcgcgg tcgacatggt ggtcgacgcg agcggcaagc cgcgcgagat gatcgtcaac ctgcaaggct tcatgggcat cggcgaccgc aaggtcatct tcccgtggaa cgtgttccgc ttcacgccgg gcggcaagca ngagccgatc gtgctcgacg tcccgtcggg cgacctgccg ccggccgcgc gaccgaaggc ggtgccgctg tcnggttcgg ccgcggcgtc gccgacnacg cggnctgccg atgctcgaca gcgatgtcna gcgcccgaac ggcacnnaga tcggccgcat cntcncgtgc tgatcgaccg cggngcacag ccgcaggccg tcgtgctcga cctcngcggg ctcgtcanta ccgaccgccg ntcgatcgcc gcgagctggg gnngcgctgc gcttcgtcnc gcgcgacaan gncgctgcnc ccgcanctcg anctgaacga ngccnnnatc aaggcatcgn cgccctacnn cggccganaa gcatcgtcgc ggncntnccn nccgtnnncc cnggccgcnn ngnnttgncn nnnnncnncc gnnnnnnnaa nnnnnacgaa caanntncnn nnnnncnann ctncnnnnnn gnnnccggcn gctaacnaan ncngaanggn nannanttng gcnnnctncc nccnnntnnn nnnnnnnnnn nnnnnnnana
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGGLPFPA SRKSLPSSTV FLILAAAALL SGCGLLPVQN PPAPISEALV ESVEETAGEP LTMPPVPAPT QPETPEAPKK PHREVTRSKP VQRPESPTPP PPPPPPIVAT RPLDRTQIHA LLDSEVARRN GKVVGRAVDM VVDASGKPRE MIVNLQGFMG IGDRKVIFPW NVFRFTPGGK QEPIVLDVPS GDLPPAARPK AVPLSGSAAA SPTTRLPMLD SDVERPNGTK IGRIVDVLID RGAQPQAVVL DLGGLVNTDR RSIAASWGAL RFVTRDKALH PQLDLNDAQI KASPPYAADK PIVAVFPPVV PAAPASSASA TR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcggcgga ctcccgtttc ccgcatcccg caagtcgttg ccgtcgtcga cggtcttcct gattctcgcc gccgccgcgc tgctgtccgg ctgcgggctg ctgccggtcc agaacccgcc cgccccgatc agcgaggcgc tcgtcgagtc ggtcgaggag accgccggcg agccgctgac gatgccgccc gtgccggcgc cgacgcaacc ggaaacgccg gaagcgccga agaagccgca ccgcgaggtg acgcggtcga aacccgtgca gcgccccgaa tcgccgaccc cgccaccccc tccgcccccg ccgatcgtcg cgacgcgccc gctcgaccgt acccagatcc acgcgctgct cgacagcgaa gtcgcgcgcc gcaacggcaa ggtggtcggc cgcgcggtcg acatggtggt cgacgcgagc ggcaagccgc gcgagatgat cgtcaacctg caaggcttca tgggcatcgg cgaccgcaag gtcatcttcc cgtggaacgt gttccgcttc acgccgggcg gcaagcagga gccgatcgtg ctcgacgtcc cgtcgggcga cctgccgccg gccgcgcgac cgaaggcggt gccgctgtcg ggttcggccg cggcgtcgcc gacgacgcgg ctgccgatgc tcgacagcga tgtcgagcgc ccgaacggca cgaagatcgg ccgcatcgtc gacgtgctga tcgaccgcgg tgcacagccg caggccgtcg tgctcgacct cggcgggctc gtcaataccg accgccgttc gatcgccgcg agctggggcg cgctgcgctt cgtcacgcgc gacaaggcgc tgcacccgca gctcgacctg aacgacgccc agatcaaggc atcgccgccc tacgcggccg acaagccgat cgtcgcggtc ttcccgcccg ttgtcccggc cgcgccggct tcgtctgcga gtgccacccg ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa