BuceA.18048.a

Probable acetoacetate decarboxylase (AAD) (ADC) (EC 4.1.1.4)

CENTER ID: BuceA.18048.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18048.a.A1.GE33234 full length 1 246
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18048.a.A1.PS01381 full length 1 246

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3695
RefSeq: YP_002232656.1
UniProt: B4EG09

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKPSDVRSKA FAMPLTSPAF PMGPYRFVDR EFLIITYRTD PDRLREIVPE PLQVTEPLVH YEFIRMADST GFGDYTESGQ VIPVEYNGQA GGYTLAMYLD DHPPIAGGRE LWGFPKKLAS PTLHVNTDHI LGTLDYGKVR VATGTMGYKH KELDIDEQTK RLAGPNFLLK IIPHVDGTAR VCELVRYYMQ DIKMKGAWTG PASLELAPHA LAPVADLPVL EIVEARHLVA DLTLGLGEVV YDYLAQ
NT Sequence
atgaaaccca gcgatgtccg atcgaaagcg tttgcgatgc cgttgaccag ccctgcgttc ccgatgggcc cgtaccgttt cgtcgatcgt gagtttctga tcatcacgta ccgcaccgat ccggaccgcc tccgtgaaat cgtccccgag ccgctgcagg tcaccgagcc gctcgtccac tacgaattca ttcgcatggc cgattcgacc ggcttcggcg attacaccga aagcggccag gtgatcccgg tcgaatacaa cggtcaggcg ggcggctata cgctcgcgat gtatctcgac gatcatccgc cgatcgccgg cggccgcgag ctgtggggct tcccgaagaa gctcgcgtcg cccacgctgc acgtgaacac cgatcacatc ctgggcacgc tcgactacgg caaggtgcgc gtcgcgaccg gcacgatggg ctacaagcac aaggaactcg acatcgacga gcagacgaag cggctcgccg gccccaattt cctgctgaag atcatcccgc acgtcgacgg caccgcgcgc gtctgcgaac tggtgcgcta ttacatgcag gacatcaaga tgaagggcgc atggacgggt cccgcatcgc tggagctggc gccgcacgcg ctcgcgccgg tggccgatct gccggtgctc gagatcgtcg aggcgcgcca cctcgtcgcg gatttgacgc tgggcctcgg cgaagtcgtc tacgattacc tggctcagta a
Details for BuceA.18048.a.A1.GE33234
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMKPSDVR SKAFAMPLTS PAFPMGPYRF VDREFLIITY RTDPDRLREI VPEPLQVTEP LVHYEFIRMA DSTGFGDYTE SGQVIPVEYN GQAGGYTLAM YLDDHPPIAG GRELWGFPKK LASPTLHVNT DHILGTLDYG KVRVATGTMG YKHKELDIDE QTKRLAGPNF LLKIIPHVDG TARVCELVRY YMQDIKMKGA WTGPASLELA PHALAPVADL PVLEIVEARH LVADLTLGLG EVVYDYLAQ
Validated NT Sequence
ttattactga gccaggtaat cgtagacgac ttcgccgagg cccagcgtca aatccgcgac gaggtggcgc gcctcgacga tctcgagcac cggcagatcg gccaccggcg cgagcgcgtg cggcgccagc tccagcgatg cgggacccgt ccatgcgccc ttcatcttga tgtcctgcat gtaatagcgc accagttcgc agacgcgcgc ggtgccgtcg acgtgcggga tgatcttcag caggaaattg gggccggcga gccgcttcgt ctgctcgtcg atgtcgagtt ccttgtgctt gtagcccatc gtgccggtcg cgacgcgcac cttgccgtag tcgagcgtgc ccaggatgtg atcggtgttc acgtgcagcg tgggcgacgc gagcttcttc gggaagcccc acagctcgcg gccgccggcg atcggcggat gatcgtcgag atacatcgcg agcgtatagc cgcccgcctg accgttgtat tcgaccggga tcacctggcc gctttcggtg taatcgccga agccggtcga atcggccatg cgaatgaatt cgtagtggac gagcggctcg gtgacctgca gcggctcggg gacgatttca cggaggcggt ccggatcggt gcggtacgtg atgatcagaa actcacgatc gacgaaacgg tacgggccca tcgggaacgc agggctggtc aacggcatcg caaacgcttt cgatcggaca tcgctgggtt tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat ga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKPSDVRSK AFAMPLTSPA FPMGPYRFVD REFLIITYRT DPDRLREIVP EPLQVTEPLV HYEFIRMADS TGFGDYTESG QVIPVEYNGQ AGGYTLAMYL DDHPPIAGGR ELWGFPKKLA SPTLHVNTDH ILGTLDYGKV RVATGTMGYK HKELDIDEQT KRLAGPNFLL KIIPHVDGTA RVCELVRYYM QDIKMKGAWT GPASLELAPH ALAPVADLPV LEIVEARHLV ADLTLGLGEV VYDYLAQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaacccagc gatgtccgat cgaaagcgtt tgcgatgccg ttgaccagcc ctgcgttccc gatgggcccg taccgtttcg tcgatcgtga gtttctgatc atcacgtacc gcaccgatcc ggaccgcctc cgtgaaatcg tccccgagcc gctgcaggtc accgagccgc tcgtccacta cgaattcatt cgcatggccg attcgaccgg cttcggcgat tacaccgaaa gcggccaggt gatcccggtc gaatacaacg gtcaggcggg cggctatacg ctcgcgatgt atctcgacga tcatccgccg atcgccggcg gccgcgagct gtggggcttc ccgaagaagc tcgcgtcgcc cacgctgcac gtgaacaccg atcacatcct gggcacgctc gactacggca aggtgcgcgt cgcgaccggc acgatgggct acaagcacaa ggaactcgac atcgacgagc agacgaagcg gctcgccggc cccaatttcc tgctgaagat catcccgcac gtcgacggca ccgcgcgcgt ctgcgaactg gtgcgctatt acatgcagga catcaagatg aagggcgcat ggacgggtcc cgcatcgctg gagctggcgc cgcacgcgct cgcgccggtg gccgatctgc cggtgctcga gatcgtcgag gcgcgccacc tcgtcgcgga tttgacgctg ggcctcggcg aagtcgtcta cgattacctg gctcagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BuceA.18048.a.A1.PS01381
PURIFICATION DATe: 2/8/2012
CONCENTRATION: 31.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMKPSDVR SKAFAMPLTS PAFPMGPYRF VDREFLIITY RTDPDRLREI VPEPLQVTEP LVHYEFIRMA DSTGFGDYTE SGQVIPVEYN GQAGGYTLAM YLDDHPPIAG GRELWGFPKK LASPTLHVNT DHILGTLDYG KVRVATGTMG YKHKELDIDE QTKRLAGPNF LLKIIPHVDG TARVCELVRY YMQDIKMKGA WTGPASLELA PHALAPVADL PVLEIVEARH LVADLTLGLG EVVYDYLAQ
Validated NT Sequence
ttattactga gccaggtaat cgtagacgac ttcgccgagg cccagcgtca aatccgcgac gaggtggcgc gcctcgacga tctcgagcac cggcagatcg gccaccggcg cgagcgcgtg cggcgccagc tccagcgatg cgggacccgt ccatgcgccc ttcatcttga tgtcctgcat gtaatagcgc accagttcgc agacgcgcgc ggtgccgtcg acgtgcggga tgatcttcag caggaaattg gggccggcga gccgcttcgt ctgctcgtcg atgtcgagtt ccttgtgctt gtagcccatc gtgccggtcg cgacgcgcac cttgccgtag tcgagcgtgc ccaggatgtg atcggtgttc acgtgcagcg tgggcgacgc gagcttcttc gggaagcccc acagctcgcg gccgccggcg atcggcggat gatcgtcgag atacatcgcg agcgtatagc cgcccgcctg accgttgtat tcgaccggga tcacctggcc gctttcggtg taatcgccga agccggtcga atcggccatg cgaatgaatt cgtagtggac gagcggctcg gtgacctgca gcggctcggg gacgatttca cggaggcggt ccggatcggt gcggtacgtg atgatcagaa actcacgatc gacgaaacgg tacgggccca tcgggaacgc agggctggtc aacggcatcg caaacgcttt cgatcggaca tcgctgggtt tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat ga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKPSDVRSK AFAMPLTSPA FPMGPYRFVD REFLIITYRT DPDRLREIVP EPLQVTEPLV HYEFIRMADS TGFGDYTESG QVIPVEYNGQ AGGYTLAMYL DDHPPIAGGR ELWGFPKKLA SPTLHVNTDH ILGTLDYGKV RVATGTMGYK HKELDIDEQT KRLAGPNFLL KIIPHVDGTA RVCELVRYYM QDIKMKGAWT GPASLELAPH ALAPVADLPV LEIVEARHLV ADLTLGLGEV VYDYLAQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAACCCAGC GATGTCCGAT CGAAAGCGTT TGCGATGCCG TTGACCAGCC CTGCGTTCCC GATGGGCCCG TACCGTTTCG TCGATCGTGA GTTTCTGATC ATCACGTACC GCACCGATCC GGACCGCCTC CGTGAAATCG TCCCCGAGCC GCTGCAGGTC ACCGAGCCGC TCGTCCACTA CGAATTCATT CGCATGGCCG ATTCGACCGG CTTCGGCGAT TACACCGAAA GCGGCCAGGT GATCCCGGTC GAATACAACG GTCAGGCGGG CGGCTATACG CTCGCGATGT ATCTCGACGA TCATCCGCCG ATCGCCGGCG GCCGCGAGCT GTGGGGCTTC CCGAAGAAGC TCGCGTCGCC CACGCTGCAC GTGAACACCG ATCACATCCT GGGCACGCTC GACTACGGCA AGGTGCGCGT CGCGACCGGC ACGATGGGCT ACAAGCACAA GGAACTCGAC ATCGACGAGC AGACGAAGCG GCTCGCCGGC CCCAATTTCC TGCTGAAGAT CATCCCGCAC GTCGACGGCA CCGCGCGCGT CTGCGAACTG GTGCGCTATT ACATGCAGGA CATCAAGATG AAGGGCGCAT GGACGGGTCC CGCATCGCTG GAGCTGGCGC CGCACGCGCT CGCGCCGGTG GCCGATCTGC CGGTGCTCGA GATCGTCGAG GCGCGCCACC TCGTCGCGGA TTTGACGCTG GGCCTCGGCG AAGTCGTCTA CGATTACCTG GCTCAGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA