SSGCID: Target Page

BuceA.18043.a

Putative saccharopine dehydrogenase

CENTER ID:	BuceA.18043.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18043.a.A1.GE33231	full length		1	366

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18043.a.A1.PW33841	full length		1	366

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.1865
RefSeq:	YP_002230922.1
UniProt:	B4E9X7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKIAIVGAGL IGHTIAHMLR ETGDYEVVAF DRDADALAKL SREGIATQRV DSADANAIRE AVKGFDALVN ALPYYLAVNV AAAAKAAGVH YFDLTEDVRA THAIRELADG SDRAFMPQCG LAPGFIGIAA HELVNGFSEV RDVKMRVGAL PEYPTNALKY NLTWSVDGLI NEYCQPCEAI RDGRKQWVQP LEGLEHFSLD GTEYEAFNTS GGLGTLCETL SGKVETLDYK SVRYPGHREL VQFLLEDLRL STDRDTLKSI MRRAVPSTKQ DVVLVFVTVT GVKDGQLVQD VFTRKIFAKD VCGMHMSAIQ ITTAGAMCAV LDLFREKKLP QSGFIPQEKV SLKAFLSNRF GKLYDGGTME RVHAVA

NT Sequence

atgaaaatcg ccattgtcgg cgcaggtctg atcggccaca ccattgctca catgctgcgc gaaacgggcg actacgaagt cgtcgcgttc gaccgcgatg cggatgcgct cgcgaagctg tcgcgcgaag gcatcgcgac gcaacgggtc gattcggccg acgcgaatgc gattcgcgaa gcagtgaagg gcttcgatgc gctcgtcaac gcgctgcctt actacctcgc ggtcaacgtg gccgccgccg cgaaggctgc cggcgttcac tacttcgacc tgaccgaaga cgtgcgtgcg acccacgcga tccgcgaact ggccgacggc tccgaccgcg cgttcatgcc gcagtgcggc ctcgcgccgg gcttcatcgg catcgccgcg cacgagctcg tgaacggctt cagcgaagtg cgcgacgtga agatgcgcgt cggcgcgctg cccgagtatc cgaccaacgc gctgaagtac aacctgacgt ggagcgtcga cggcctgatc aacgagtact gccagccgtg cgaagcgatc cgcgacggcc gcaagcagtg ggtgcagccg ctcgaaggcc tcgagcactt ctcgctcgac ggcaccgaat acgaagcgtt caacacgtcg ggcggcctcg gcacgctgtg cgaaacgctg tcgggcaagg tcgagacgct cgactacaag tcggtgcgct acccgggtca ccgcgagctc gttcagttcc tgctggaaga cctgcgcctg tcgaccgatc gcgacacgct gaagtcgatc atgcgccgcg cggtgccgtc gaccaagcag gacgtcgtgc tcgtgttcgt cacggtgacg ggcgtgaagg acggccagct ggtgcaggac gtgttcaccc gcaagatctt cgcgaaggac gtgtgcggga tgcacatgag cgcgatccag atcacgacgg ccggcgcgat gtgcgcggtg ctggatctgt tccgcgaaaa gaagctgccg cagagcggtt tcatcccgca agagaaggtg tcgctgaagg cgttcctgtc gaaccgcttc ggcaagctgt acgacggcgg cacgatggag cgcgtgcacg cggttgcctg a

Details for BuceA.18043.a.A1.GE33231

HARVESTED ON:	8/30/2011
SEQUENCED ON:	2/4/2013
EXPECTED MW:	42kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

gggtaccctg gaagctcaga cccagggtcc tggttcgatg aaaatcgcca ttgtcggcgc aggtctgatc ggccacacca ttgctcacat gctgcgcgaa acgggcgact acgaagtcgt cgcgttcgac cgcgatgcgg atgcgctcgc gaagctgtcg cgcgaaggca tcgcgacgca acgggtcgat tcggccgacg cgaatgcgat tcgcgaagca gtgaagggct tcgatgcgct cgtcaacgcg ctgccttact acctcgcggt caacgtggcc gccgccgcga aggctgccgg cgttcactac ttcgacctga ccgaagacgt gcgtgcgacc cacgcgatcc gcgaactggc cgacggctcc gaccgcgcgt tcatgccgca gtgcggcctc gcgccgggct tcatcggcat cgccgcgcac gagctcgtga acggcttcag cgaagtgcgc gacgtgaaga tgcgcgtcgg cgcgctgccc gagtatccga ccaacgcgct gaagtacaac ctgacgtgga gcgtcgacgg cctgatcaac gagtactgcc agccgtgcga agcgatccgc gacggccgca agcagtgggt gcagccgctc gaaggcctcg agcacttctc gctcgacggc accgaatacg aagcgttcaa cacgtcgggc ggcctcggca cgctgtgcga aacgctgtcg ggcaaggtcg agacgctcga ctacaagtcg gtgcgctacc cgggtcaccg cgagctcgtt cagttcctgc tggaagacct gcgcctgtcg accgatcgcg acacgctgaa gtcgatcatg cgccgcgcgg tgccgtcgac caagcaggac gtcgtgctcg tgttcgtcac ggtgacgggc gtgaaggacg gccagctggt gcaggacgtg ttcacccgca agatcttcgc gaaggacgtg tgcgggatgc acatgagcgc gatccagatc acgacggccg gcgcgatgtg cgcggtgctg gatctgttcc gcgaaaagaa gctgccgcag agcggtttca tcccgcaaga gaaggtgtcg ctgaaggcgt tcctgtcgaa ccgcttcggc aagctgtacg acggcggcac gatggagcgc gtgcacgcgg ttgccta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKIAIVGAG LIGHTIAHML RETGDYEVVA FDRDADALAK LSREGIATQR VDSADANAIR EAVKGFDALV NALPYYLAVN VAAAAKAAGV HYFDLTEDVR ATHAIRELAD GSDRAFMPQC GLAPGFIGIA AHELVNGFSE VRDVKMRVGA LPEYPTNALK YNLTWSVDGL INEYCQPCEA IRDGRKQWVQ PLEGLEHFSL DGTEYEAFNT SGGLGTLCET LSGKVETLDY KSVRYPGHRE LVQFLLEDLR LSTDRDTLKS IMRRAVPSTK QDVVLVFVTV TGVKDGQLVQ DVFTRKIFAK DVCGMHMSAI QITTAGAMCA VLDLFREKKL PQSGFIPQEK VSLKAFLSNR FGKLYDGGTM ERVHAVA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaatcgcc attgtcggcg caggtctgat cggccacacc attgctcaca tgctgcgcga aacgggcgac tacgaagtcg tcgcgttcga ccgcgatgcg gatgcgctcg cgaagctgtc gcgcgaaggc atcgcgacgc aacgggtcga ttcggccgac gcgaatgcga ttcgcgaagc agtgaagggc ttcgatgcgc tcgtcaacgc gctgccttac tacctcgcgg tcaacgtggc cgccgccgcg aaggctgccg gcgttcacta cttcgacctg accgaagacg tgcgtgcgac ccacgcgatc cgcgaactgg ccgacggctc cgaccgcgcg ttcatgccgc agtgcggcct cgcgccgggc ttcatcggca tcgccgcgca cgagctcgtg aacggcttca gcgaagtgcg cgacgtgaag atgcgcgtcg gcgcgctgcc cgagtatccg accaacgcgc tgaagtacaa cctgacgtgg agcgtcgacg gcctgatcaa cgagtactgc cagccgtgcg aagcgatccg cgacggccgc aagcagtggg tgcagccgct cgaaggcctc gagcacttct cgctcgacgg caccgaatac gaagcgttca acacgtcggg cggcctcggc acgctgtgcg aaacgctgtc gggcaaggtc gagacgctcg actacaagtc ggtgcgctac ccgggtcacc gcgagctcgt tcagttcctg ctggaagacc tgcgcctgtc gaccgatcgc gacacgctga agtcgatcat gcgccgcgcg gtgccgtcga ccaagcagga cgtcgtgctc gtgttcgtca cggtgacggg cgtgaaggac ggccagctgg tgcaggacgt gttcacccgc aagatcttcg cgaaggacgt gtgcgggatg cacatgagcg cgatccagat cacgacggcc ggcgcgatgt gcgcggtgct ggatctgttc cgcgaaaaga agctgccgca gagcggtttc atcccgcaag agaaggtgtc gctgaaggcg ttcctgtcga accgcttcgg caagctgtac gacggcggca cgatggagcg cgtgcacgcg gttgcctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BuceA.18043.a.A1.PW33841

PURIFICATION DATe:	10/14/2011
CONCENTRATION:	25.14mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAATCGCC ATTGTCGGCG CAGGTCTGAT CGGCCACACC ATTGCTCACA TGCTGCGCGA AACGGGCGAC TACGAAGTCG TCGCGTTCGA CCGCGATGCG GATGCGCTCG CGAAGCTGTC GCGCGAAGGC ATCGCGACGC AACGGGTCGA TTCGGCCGAC GCGAATGCGA TTCGCGAAGC AGTGAAGGGC TTCGATGCGC TCGTCAACGC GCTGCCTTAC TACCTCGCGG TCAACGTGGC CGCCGCCGCG AAGGCTGCCG GCGTTCACTA CTTCGACCTG ACCGAAGACG TGCGTGCGAC CCACGCGATC CGCGAACTGG CCGACGGCTC CGACCGCGCG TTCATGCCGC AGTGCGGCCT CGCGCCGGGC TTCATCGGCA TCGCCGCGCA CGAGCTCGTG AACGGCTTCA GCGAAGTGCG CGACGTGAAG ATGCGCGTCG GCGCGCTGCC CGAGTATCCG ACCAACGCGC TGAAGTACAA CCTGACGTGG AGCGTCGACG GCCTGATCAA CGAGTACTGC CAGCCGTGCG AAGCGATCCG CGACGGCCGC AAGCAGTGGG TGCAGCCGCT CGAAGGCCTC GAGCACTTCT CGCTCGACGG CACCGAATAC GAAGCGTTCA ACACGTCGGG CGGCCTCGGC ACGCTGTGCG AAACGCTGTC GGGCAAGGTC GAGACGCTCG ACTACAAGTC GGTGCGCTAC CCGGGTCACC GCGAGCTCGT TCAGTTCCTG CTGGAAGACC TGCGCCTGTC GACCGATCGC GACACGCTGA AGTCGATCAT GCGCCGCGCG GTGCCGTCGA CCAAGCAGGA CGTCGTGCTC GTGTTCGTCA CGGTGACGGG CGTGAAGGAC GGCCAGCTGG TGCAGGACGT GTTCACCCGC AAGATCTTCG CGAAGGACGT GTGCGGGATG CACATGAGCG CGATCCAGAT CACGACGGCC GGCGCGATGT GCGCGGTGCT GGATCTGTTC CGCGAAAAGA AGCTGCCGCA GAGCGGTTTC ATCCCGCAAG AGAAGGTGTC GCTGAAGGCG TTCCTGTCGA ACCGCTTCGG CAAGCTGTAC GACGGCGGCA CGATGGAGCG CGTGCACGCG GTTGCCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA