BuceA.18041.a

Putative type VI secretion system protein TssG

CENTER ID: BuceA.18041.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18041.a.A1.GE33230 full length 1 366
BuceA.18041.a.B2.GE42110 PF06996_0 DUF 20 344
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.363
RefSeq: YP_002229512.1
UniProt: B4E6R8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQAPNRRIDP GVVDALLDEP HRFEFFQAVR VLEGLFARQA SDAPGAWRQG DVVAQRIAFR NTLSLGFPPS EIEGARSFDD DGASLSSDEQ RGAALAAGEL GRVELTPAFF GLLGGQGALP LHYTEQIVAR EHLRRDHAAR AFFDVFSNRA TALFYAAWKK YRLPFHYELD RDERYLPLLL AIAGVPSDEV RDSLAAGAGG VLDEAVAGYA LAARHRPMSA AYLQRTLSDY FRVPVKIDQF VGKWYDVPPD QLSVLGEVNA VLGATALVGE RVWQRDMRAR IVVGPLSKRD YEAFLPGGAQ AVALERMLTL LAGVTLEYEV KLVLKRTEVG ASVLGAGSRL GWDAFLCTRD AAEDRSDARY ELHVIH
NT Sequence
atgcaagccc cgaaccggcg aatcgatcct ggcgtagtcg acgcgctgct cgacgagccg caccgcttcg agttcttcca ggcggtgcgc gtgctcgagg ggctgttcgc gcggcaggcg tcggatgcgc ccggcgcatg gcggcagggc gacgtggtcg cgcagcgcat cgcattccgc aacacgctgt cgctcggctt tccgccgagc gagatcgaag gcgcgcgctc gttcgacgac gacggcgcgt cgctgagttc ggacgagcag cgcggcgccg cgctggccgc cggcgagctc ggccgcgtcg agctgacgcc cgcgttcttc gggctgctcg gcgggcaagg cgcgctgccg ctgcactaca ccgagcagat cgtcgcgcgc gagcatctgc ggcgcgacca tgcggcgcgc gcgttcttcg acgtgttctc gaaccgcgcg accgcgctgt tctacgcggc gtggaagaaa taccggctgc cgttccacta cgagctcgac cgcgacgagc gctacctgcc gctgttgctc gcgatcgcgg gcgtgccgag cgacgaggtg cgcgacagcc tcgcggccgg cgcgggcggt gtgctcgacg aggccgtggc cggctacgcg ctcgccgcgc ggcaccggcc gatgtcggcc gcctacctgc agcgcacgct gtccgattac ttccgcgtgc cggtgaagat cgaccagttc gtcggcaagt ggtacgacgt gccgcccgat cagctgagcg tgctcggtga agtcaacgcg gtgctcggcg cgacggcgct cgtcggcgag cgcgtgtggc agcgcgacat gcgcgcgcga atcgtcgtcg gcccgctgtc caagcgcgac tacgaggcgt tcctgcccgg cggcgcgcag gccgtcgcgc tcgagcggat gctgacgctg ctcgccggcg tcacgctcga atacgaggtg aagctcgtgc tgaagcgcac cgaggtcggc gcgagcgtgc tcggcgcggg ctcgcggctc ggctgggatg cgttcctgtg cacgcgcgac gcggccgagg accgttcgga cgcccgctac gaactgcacg tgattcactg a
Details for BuceA.18041.a.A1.GE33230
HARVESTED ON: 8/30/2011
SEQUENCED ON: 3/27/2014
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 79
Validated AA Sequence
MQAPNRRIDP GVVDALLDEP HRFEFFQAVR VLEGLFARQA SDAPGAWRQG DVVAQRIAFR NTLSLGFPPS EIEGARSFDD DGASLSSDEQ RGAALAAGEL GRVELTPAFF GLLGGQGALP LHYTEQIVAR EHLRRDHAAR AFFDVFSNRA TALFYAAWKK YRLPFHYELD RDERYLPLLL AIAGVPSDEV RDSLAAGAGG VLDEAVAGYA LAARHRPMSA AYLQRTLSDY FRVPVKIDQF VGKWYDVPPD QLSVLGEVNA XLGATALVGE RVWXRDXRAR IVVXXLSKRD YXXFLPXGXX XSRSXXC
Validated NT Sequence
gggtaccctg gaagctcaga cccagggtcc tggttcgatg caagccccga accggcgaat cgatcctggc gtagtcgacg cgctgctcga cgagccgcac cgcttcgagt tcttccaggc ggtgcgcgtg ctcgaggggc tgttcgcgcg gcaggcgtcg gatgcgcccg gcgcatggcg gcagggcgac gtggtcgcgc agcgcatcgc attccgcaac acgctgtcgc tcggctttcc gccgagcgag atcgaaggcg cgcgctcgtt cgacgacgac ggcgcgtcgc tgagttcgga cgagcagcgc ggcgccgcgc tggccgccgg cgagctcggc cgcgtcgagc tgacgcccgc gttcttcggg ctgctcggcg ggcaaggcgc gctgccgctg cactacaccg agcagatcgt cgcgcgcgag catctgcggc gcgaccatgc ggcgcgcgcg ttcttcgacg tgttctcgaa ccgcgcgacc gcgctgttct acgcggcgtg gaagaaatac cggctgccgt tccactacga gctcgaccgc gacgagcgct acctgccgct gttgctcgcg atcgcgggcg tgccgagcga cgaggtgcgc gacagcctcg cggccggcgc gggcggtgtg ctcgacgagg ccgtggccgg ctacgcgctc gccgcgcggc accggccgat gtcggccgcc tacctgcagc gcacgctgtc cgattacttc cgcgtgccgg tgaagatcga ccagttcgtc ggcaagtggt acgacgtgcc gcccgatcag ctgagcgtgc tcggtgaagt caacgcggng ctcggcgcga cggcgctcgt cggcgagcgc gtgtggnagc gcgacntgcg cgcgcgaatc gtcgtnngcc nnctgtccaa gcgcgactac nagnnnttcc tgccnngcgg cncgcnnncg tcgcgctcna ncgnntgctg annc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQAPNRRID PGVVDALLDE PHRFEFFQAV RVLEGLFARQ ASDAPGAWRQ GDVVAQRIAF RNTLSLGFPP SEIEGARSFD DDGASLSSDE QRGAALAAGE LGRVELTPAF FGLLGGQGAL PLHYTEQIVA REHLRRDHAA RAFFDVFSNR ATALFYAAWK KYRLPFHYEL DRDERYLPLL LAIAGVPSDE VRDSLAAGAG GVLDEAVAGY ALAARHRPMS AAYLQRTLSD YFRVPVKIDQ FVGKWYDVPP DQLSVLGEVN AVLGATALVG ERVWQRDMRA RIVVGPLSKR DYEAFLPGGA QAVALERMLT LLAGVTLEYE VKLVLKRTEV GASVLGAGSR LGWDAFLCTR DAAEDRSDAR YELHVIH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaagccccg aaccggcgaa tcgatcctgg cgtagtcgac gcgctgctcg acgagccgca ccgcttcgag ttcttccagg cggtgcgcgt gctcgagggg ctgttcgcgc ggcaggcgtc ggatgcgccc ggcgcatggc ggcagggcga cgtggtcgcg cagcgcatcg cattccgcaa cacgctgtcg ctcggctttc cgccgagcga gatcgaaggc gcgcgctcgt tcgacgacga cggcgcgtcg ctgagttcgg acgagcagcg cggcgccgcg ctggccgccg gcgagctcgg ccgcgtcgag ctgacgcccg cgttcttcgg gctgctcggc gggcaaggcg cgctgccgct gcactacacc gagcagatcg tcgcgcgcga gcatctgcgg cgcgaccatg cggcgcgcgc gttcttcgac gtgttctcga accgcgcgac cgcgctgttc tacgcggcgt ggaagaaata ccggctgccg ttccactacg agctcgaccg cgacgagcgc tacctgccgc tgttgctcgc gatcgcgggc gtgccgagcg acgaggtgcg cgacagcctc gcggccggcg cgggcggtgt gctcgacgag gccgtggccg gctacgcgct cgccgcgcgg caccggccga tgtcggccgc ctacctgcag cgcacgctgt ccgattactt ccgcgtgccg gtgaagatcg accagttcgt cggcaagtgg tacgacgtgc cgcccgatca gctgagcgtg ctcggtgaag tcaacgcggt gctcggcgcg acggcgctcg tcggcgagcg cgtgtggcag cgcgacatgc gcgcgcgaat cgtcgtcggc ccgctgtcca agcgcgacta cgaggcgttc ctgcccggcg gcgcgcaggc cgtcgcgctc gagcggatgc tgacgctgct cgccggcgtc acgctcgaat acgaggtgaa gctcgtgctg aagcgcaccg aggtcggcgc gagcgtgctc ggcgcgggct cgcggctcgg ctgggatgcg ttcctgtgca cgcgcgacgc ggccgaggac cgttcggacg cccgctacga actgcacgtg attcactaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BuceA.18041.a.B2.GE42110
HARVESTED ON: 1/16/2018
SEQUENCED ON: 1/17/2018
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMP HRXXFFQAVR VLEGLFARXA SDAPGAXXQG DVVAQRIAFR NTLSLGFPPS EIEGARSFDD YGASLSSDEQ RGXXLAAGEL GRVELTPAFF GLLGGQGALP LHYTEQIVAR EHLRRDHAAR AFFDVFSNRA TALFYAAWKK YRLPFHYELD RDERYLPLLL AIAGVPSDEV RDSLAAGAGG VLDEAVAGYA LAARHRPMSA AYLQRTLSDY FRVPVKIDQF VGKWYDVPPD QLSVLGEVNA VLGATALVGE RVWQRDMRAR IVVGPLSKRD YEAFLPGGAQ AVALERMLTL LAGVTLEYEV KLVLKRTEVG ASVLG
Validated NT Sequence
atggctcacc accaccacca ccatatgccg caccgcttnn ngttcttcca ggcggtgcgc gtgctcgagg ggctgttcgc gcggnnngcg tcggatgcgc ccggcgcatg nnnncagggc gacgtggtcg cgcagcgcat cgcattccgc aacacgctgt cgctcggctt tccgccgagc gagatcgaag gcgcgcgctc gttcgacgac tacggcgcgt cgctgagttc ggacgagcag cgcggcgnnn ngctggccgc cggcgagctc ggccgcgtcg agctgacgcc cgcgttcttc gggctgctcg gcgggcaagg cgcgctgccg ctgcactaca ccgagcagat cgtcgcgcgc gagcatctgc ggcgcgacca tgcggcgcgc gcgttcttcg acgtgttctc gaaccgcgcg accgcgctgt tctacgcggc gtggaagaaa taccggctgc cgttccacta cgagctcgac cgcgacgagc gctacctgcc gctgttgctc gcgatcgcgg gcgtgccgag cgacgaggtg cgcgacagcc tcgcggccgg cgcgggcggt gtgctcgacg aggccgtggc cggctacgcg ctcgccgcgc ggcaccggcc gatgtcggcc gcctacctgc agcgcacgct gtccgattac ttccgcgtgc cggtgaagat cgaccagttc gtcggcaagt ggtacgacgt gccgcccgat cagctgagcg tgctcggtga agtcaacgcg gtgctcggcg cgacggcgct cgtcggcgag cgcgtgtggc agcgcgacat gcgcgcgcga atcgtcgtcg gcccgctgtc caagcgcgac tacgaggcgt tcctgcccgg cggcgcgcag gccgtcgcgc tcgagcggat gctgacgctg ctcgccggcg tcacgctcga atacgaggtg aagctcgtgc tgaagcgcac cgaggtcggc gcgagcgtgc tcggcgcg
Expected Protein Sequence
MAHHHHHHPH RFEFFQAVRV LEGLFARQAS DAPGAWRQGD VVAQRIAFRN TLSLGFPPSE IEGARSFDDD GASLSSDEQR GAALAAGELG RVELTPAFFG LLGGQGALPL HYTEQIVARE HLRRDHAARA FFDVFSNRAT ALFYAAWKKY RLPFHYELDR DERYLPLLLA IAGVPSDEVR DSLAAGAGGV LDEAVAGYAL AARHRPMSAA YLQRTLSDYF RVPVKIDQFV GKWYDVPPDQ LSVLGEVNAV LGATALVGER VWQRDMRARI VVGPLSKRDY EAFLPGGAQA VALERMLTLL AGVTLEYEVK LVLKRTEVGA SVLGAGSRLG WDA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccgcacc gcttcgagtt cttccaggcg gtgcgcgtgc tcgaggggct gttcgcgcgg caggcgtcgg atgcgcccgg cgcatggcgg cagggcgacg tggtcgcgca gcgcatcgca ttccgcaaca cgctgtcgct cggctttccg ccgagcgaga tcgaaggcgc gcgctcgttc gacgacgacg gcgcgtcgct gagttcggac gagcagcgcg gcgccgcgct ggccgccggc gagctcggcc gcgtcgagct gacgcccgcg ttcttcgggc tgctcggcgg gcaaggcgcg ctgccgctgc actacaccga gcagatcgtc gcgcgcgagc atctgcggcg cgaccatgcg gcgcgcgcgt tcttcgacgt gttctcgaac cgcgcgaccg cgctgttcta cgcggcgtgg aagaaatacc ggctgccgtt ccactacgag ctcgaccgcg acgagcgcta cctgccgctg ttgctcgcga tcgcgggcgt gccgagcgac gaggtgcgcg acagcctcgc ggccggcgcg ggcggtgtgc tcgacgaggc cgtggccggc tacgcgctcg ccgcgcggca ccggccgatg tcggccgcct acctgcagcg cacgctgtcc gattacttcc gcgtgccggt gaagatcgac cagttcgtcg gcaagtggta cgacgtgccg cccgatcagc tgagcgtgct cggtgaagtc aacgcggtgc tcggcgcgac ggcgctcgtc ggcgagcgcg tgtggcagcg cgacatgcgc gcgcgaatcg tcgtcggccc gctgtccaag cgcgactacg aggcgttcct gcccggcggc gcgcaggccg tcgcgctcga gcggatgctg acgctgctcg ccggcgtcac gctcgaatac gaggtgaagc tcgtgctgaa gcgcaccgag gtcggcgcga gcgtgctcgg cgcgggctcg cggctcggct gggatgcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat