SSGCID: Target Page

BuceA.18039.a

Putative type VI secretion system protein TssA

CENTER ID:	BuceA.18039.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18039.a.A1.GE33229	full length		1	373

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18039.a.A1.PW33839	full length		1	373

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.365
RefSeq:	YP_002229514.1
UniProt:	B4E6S0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPINLPELLT PISEASPSGD DLLFSNEFDA IQDARRYDDP TLDQGEWVTE IKEADWGFVV DHAGELLRTR TKDLRLAVWL TEALALEDGI TGLTEGYALL EGLCREFWDT FHPLPEDDDI EHRLGNVAWL SGRTAELLRA VPLTDGASNA FSTLDWEVAQ HVAQSIKRDP EHADDIARGK PSIEQIDASR RVTSIAFYTA LLANLKAFEF ALDAFEERLV ERAGDSAPSF RQARDAFETV YRLAERFARE QGYTGSAPHT QAVQQAQPER IEPVFGQPIQ TEETHVQQQT ASRPPVTQTI AGIQNRAQAV DQLRAVARYF RQTEPHSPVA YLADKAAEWA DMPLHKWLES VVKDDGSLSH IRELLGVRPD EQS

NT Sequence

atgccgatca atctccccga gctgctgacg ccgatcagcg aggcgtcgcc cagcggcgac gacctgctgt tctcgaacga attcgacgcg atccaggacg cgcggcgcta cgacgacccg acgctcgacc agggcgaatg ggtgaccgag atcaaggagg ccgactgggg cttcgtcgtc gaccatgcgg gcgagctgct gcgcacgcgc acgaaggacc tgcggctcgc cgtgtggctg accgaggcgc tcgcgctcga ggacggcatc accggcctca ccgaaggcta tgcgctgctc gagggtctgt gccgcgagtt ctgggacacc ttccacccgc tgcccgagga cgacgacatc gagcaccggc tcggcaacgt cgcatggctg tccggtcgca cggccgagct gctgcgcgcg gtgccgctga ccgacggtgc atcgaatgcg ttcagcacgc tcgactggga agtcgcgcag cacgtcgcgc agtcgatcaa gcgcgacccc gagcatgcgg acgacatcgc gcgcggcaag ccgtcgatcg agcagatcga cgcgtcgcgg cgcgtgacgt cgatcgcgtt ctacacggcg ctgctggcga acctgaaggc gttcgaattc gcgctcgatg cgttcgagga gcggctcgtc gagcgcgcgg gcgattcggc gccgagcttc cggcaggcgc gcgacgcgtt cgagaccgtg taccggctcg ccgagcgctt cgcgcgcgaa cagggctata ccggcagcgc gccgcacacg caggcggtgc agcaggccca gcccgagcgc atcgagccgg tgttcggcca accgatccag accgaggaga cccacgtgca gcagcagacc gcttcgcgtc ctccggtgac gcagacgatc gccggcatcc agaaccgtgc gcaggccgtc gaccagttgc gtgcggtggc gcgctatttc cgtcagaccg agccgcacag cccagtcgcg tatctcgccg acaaagcggc cgaatgggcc gacatgccgc tgcacaagtg gctcgagagc gtcgtgaagg acgacggctc gctgtcgcat attcgcgaac tgctgggcgt gcggcccgac gagcagtcgt aa

Details for BuceA.18039.a.A1.GE33229

HARVESTED ON:	8/30/2011
SEQUENCED ON:	3/27/2014
EXPECTED MW:	44kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMPINLPE LLTPISEASP SGDDLLFSNE FDAIQDARRY DDPTLDQGEW VTEIKEADWG FVVDHAGELL RTRTKDLRLA VWLTEALALE DGITGLTEGY ALLEGLCREF WDTFHPLPED DDIEHRLGNV AWLSGRTAEL LRAVPLTDGA SNAFSTLDWE VAQHVAQSIK RDPEHADDIA RGKPSIEQID ASRRVTSIAF YTALLANLKA FEFALDAFEE RLVERAGDSA PSFRQARDAF ETVYRLAERF AREQGYTGSA PHTQAVQQAQ PERIEPVFGQ PIQTEETHVQ QQTASRPPVT QTIAGIQNRA QAVDQLRAVA RYFRQTEPHS PVAYLADKAA EWADMPLHKW LESVVKDDGS LSHIRELLGV RPD

Validated NT Sequence

caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgccg atcaatctcc ccgagctgct gacgccgatc agcgaggcgt cgcccagcgg cgacgacctg ctgttctcga acgaattcga cgcgatccag gacgcgcggc gctacgacga cccgacgctc gaccagggcg aatgggtgac cgagatcaag gaggccgact ggggcttcgt cgtcgaccat gcgggcgagc tgctgcgcac gcgcacgaag gacctgcggc tcgccgtgtg gctgaccgag gcgctcgcgc tcgaggacgg catcaccggc ctcaccgaag gctatgcgct gctcgagggt ctgtgccgcg agttctggga caccttccac ccgctgcccg aggacgacga catcgagcac cggctcggca acgtcgcatg gctgtccggt cgcacggccg agctgctgcg cgcggtgccg ctgaccgacg gtgcatcgaa tgcgttcagc acgctcgact gggaagtcgc gcagcacgtc gcgcagtcga tcaagcgcga ccccgagcat gcggacgaca tcgcgcgcgg caagccgtcg atcgagcaga tcgacgcgtc gcggcgcgtg acgtcgatcg cgttctacac ggcgctgctg gcgaacctga aggcgttcga attcgcgctc gatgcgttcg aggagcggct cgtcgagcgc gcgggcgatt cggcgccgag cttccggcag gcgcgcgacg cgttcgagac cgtgtaccgg ctcgccgagc gcttcgcgcg cgaacagggc tataccggca gcgcgccgca cacgcaggcg gtgcagcagg cccagcccga gcgcatcgag ccggtgttcg gccaaccgat ccagaccgag gagacccacg tgcagcagca gaccgcttcg cgtcctccgg tgacgcagac gatcgccggc atccagaacc gtgcgcaggc cgtcgaccag ttgcgtgcgg tggcgcgcta tttccgtcag accgagccgc acagcccagt cgcgtatctc gccgacaaag cggccgaatg ggccgacatg ccgctgcaca agtggctcga gagcgtcgtg aaggacgacg gctcgctgtc gcatattcgc gaactgctgg gcgtgcggcc cgacgag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPINLPELL TPISEASPSG DDLLFSNEFD AIQDARRYDD PTLDQGEWVT EIKEADWGFV VDHAGELLRT RTKDLRLAVW LTEALALEDG ITGLTEGYAL LEGLCREFWD TFHPLPEDDD IEHRLGNVAW LSGRTAELLR AVPLTDGASN AFSTLDWEVA QHVAQSIKRD PEHADDIARG KPSIEQIDAS RRVTSIAFYT ALLANLKAFE FALDAFEERL VERAGDSAPS FRQARDAFET VYRLAERFAR EQGYTGSAPH TQAVQQAQPE RIEPVFGQPI QTEETHVQQQ TASRPPVTQT IAGIQNRAQA VDQLRAVARY FRQTEPHSPV AYLADKAAEW ADMPLHKWLE SVVKDDGSLS HIRELLGVRP DEQS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgatcaat ctccccgagc tgctgacgcc gatcagcgag gcgtcgccca gcggcgacga cctgctgttc tcgaacgaat tcgacgcgat ccaggacgcg cggcgctacg acgacccgac gctcgaccag ggcgaatggg tgaccgagat caaggaggcc gactggggct tcgtcgtcga ccatgcgggc gagctgctgc gcacgcgcac gaaggacctg cggctcgccg tgtggctgac cgaggcgctc gcgctcgagg acggcatcac cggcctcacc gaaggctatg cgctgctcga gggtctgtgc cgcgagttct gggacacctt ccacccgctg cccgaggacg acgacatcga gcaccggctc ggcaacgtcg catggctgtc cggtcgcacg gccgagctgc tgcgcgcggt gccgctgacc gacggtgcat cgaatgcgtt cagcacgctc gactgggaag tcgcgcagca cgtcgcgcag tcgatcaagc gcgaccccga gcatgcggac gacatcgcgc gcggcaagcc gtcgatcgag cagatcgacg cgtcgcggcg cgtgacgtcg atcgcgttct acacggcgct gctggcgaac ctgaaggcgt tcgaattcgc gctcgatgcg ttcgaggagc ggctcgtcga gcgcgcgggc gattcggcgc cgagcttccg gcaggcgcgc gacgcgttcg agaccgtgta ccggctcgcc gagcgcttcg cgcgcgaaca gggctatacc ggcagcgcgc cgcacacgca ggcggtgcag caggcccagc ccgagcgcat cgagccggtg ttcggccaac cgatccagac cgaggagacc cacgtgcagc agcagaccgc ttcgcgtcct ccggtgacgc agacgatcgc cggcatccag aaccgtgcgc aggccgtcga ccagttgcgt gcggtggcgc gctatttccg tcagaccgag ccgcacagcc cagtcgcgta tctcgccgac aaagcggccg aatgggccga catgccgctg cacaagtggc tcgagagcgt cgtgaaggac gacggctcgc tgtcgcatat tcgcgaactg ctgggcgtgc ggcccgacga gcagtcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for BuceA.18039.a.A1.PW33839

PURIFICATION DATe:	11/19/2011
CONCENTRATION:	23.66mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	50µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGATCAAT CTCCCCGAGC TGCTGACGCC GATCAGCGAG GCGTCGCCCA GCGGCGACGA CCTGCTGTTC TCGAACGAAT TCGACGCGAT CCAGGACGCG CGGCGCTACG ACGACCCGAC GCTCGACCAG GGCGAATGGG TGACCGAGAT CAAGGAGGCC GACTGGGGCT TCGTCGTCGA CCATGCGGGC GAGCTGCTGC GCACGCGCAC GAAGGACCTG CGGCTCGCCG TGTGGCTGAC CGAGGCGCTC GCGCTCGAGG ACGGCATCAC CGGCCTCACC GAAGGCTATG CGCTGCTCGA GGGTCTGTGC CGCGAGTTCT GGGACACCTT CCACCCGCTG CCCGAGGACG ACGACATCGA GCACCGGCTC GGCAACGTCG CATGGCTGTC CGGTCGCACG GCCGAGCTGC TGCGCGCGGT GCCGCTGACC GACGGTGCAT CGAATGCGTT CAGCACGCTC GACTGGGAAG TCGCGCAGCA CGTCGCGCAG TCGATCAAGC GCGACCCCGA GCATGCGGAC GACATCGCGC GCGGCAAGCC GTCGATCGAG CAGATCGACG CGTCGCGGCG CGTGACGTCG ATCGCGTTCT ACACGGCGCT GCTGGCGAAC CTGAAGGCGT TCGAATTCGC GCTCGATGCG TTCGAGGAGC GGCTCGTCGA GCGCGCGGGC GATTCGGCGC CGAGCTTCCG GCAGGCGCGC GACGCGTTCG AGACCGTGTA CCGGCTCGCC GAGCGCTTCG CGCGCGAACA GGGCTATACC GGCAGCGCGC CGCACACGCA GGCGGTGCAG CAGGCCCAGC CCGAGCGCAT CGAGCCGGTG TTCGGCCAAC CGATCCAGAC CGAGGAGACC CACGTGCAGC AGCAGACCGC TTCGCGTCCT CCGGTGACGC AGACGATCGC CGGCATCCAG AACCGTGCGC AGGCCGTCGA CCAGTTGCGT GCGGTGGCGC GCTATTTCCG TCAGACCGAG CCGCACAGCC CAGTCGCGTA TCTCGCCGAC AAAGCGGCCG AATGGGCCGA CATGCCGCTG CACAAGTGGC TCGAGAGCGT CGTGAAGGAC GACGGCTCGC TGTCGCATAT TCGCGAACTG CTGGGCGTGC GGCCCGACGA GCAGTCGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA