BuceA.18032.a

ClpB heat-shock protein

CENTER ID: BuceA.18032.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18032.a.A2.GE33226 uvrD helicase ATP-binding domain 185 775
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18032.a.A2.PS01284 uvrD helicase ATP-binding domain 185 775

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1993
RefSeq: YP_002231047.1
UniProt: B4EB05

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRIDKLTTKF QEALADAQSL AAGRDNQYIE PVHVLAALVA QQDGSARSLM SRAGVHVQAL QGALNEAISR LPQVTGTGGD IQIGRELAGL LNQADKEAQK LNDTFIASEM FLLAVSDDKG DAGKLARQHG LTRKALEAAI AAVRGGSQVH SQDAESQREA LKKYTVDLTE RARAGKLDPV IGRDDEIRRS IQILQRRTKN NPVLIGEPGV GKTAIVEGLA QRIVNGEVPE TLKGKRVLSL DMAALLAGAK YRGEFEERLK AVLNDIAKDE GQTIVFIDEI HTMVGAGKAE GAMDAGNMLK PALSRGELHC IGATTLDEYR KYIEKDAALE RRFQKVLVDE PSVEATIAIL RGLQEKYELH HGVDITDPAI VAAAELSHRY ITDRFLPDKA IDLIDEAASK IKMEIDSKPE EMDKLDRRLI QLKIEREAVK KEQDEASQKR LQLIEEEIER LGREYADLEE IWTAEKAAVQ GSAQLKEEIE KVRADIARLQ REGKLEKVAE LQYGKLPQLE SQLKQVTQAE EQEQHNPTRP RLLRTQVGAE EIAEVVSRST GIPVSRMMQG EREKLLHIEE KLHERVVGQD EAISAVADAI RRSRAGLADP NRPYGSFLFL GPTGVGKTEL CKALASFLFD SEEHLIRIDM SEFMEKHSVA RLIGAPPGYV GYEEGGYLTE AVRRKPYSVI LLDEIEKANP DVFNVLLQVL DDGRMTDGQG RTVDFKNTVI VMTSNLGSQV IQAMTGSPQD EIKDAVWLEV KQHFRPEFLN RIDDVVVFHA LDRSNIESIA KIQLARLHDR LAKLDMALDV SPAALEQIAK VGYDPVFGAR PLKRAIQQEI ENPVAKLILA GRFGPKDVIP VDVNDGQFVF DRVVH
NT Sequence
atgagaatcg acaagctcac caccaagttc caggaagcac tcgcggacgc gcaaagcctc gcggcaggcc gcgacaatca atacatcgaa cccgttcacg tcctcgcggc gctggtcgcg caacaggacg gctcggcccg ctcgctgatg tcgcgtgcgg gcgtgcacgt gcaggcgctg caaggcgcgc tgaacgaagc catctcgcgc ctgccgcagg tgacgggcac gggcggcgac atccagatcg gccgtgagtt ggccgggctg ctgaaccagg ccgacaagga agcgcagaag ctcaacgaca cgttcatcgc gagcgagatg ttcctgctcg cggtgtcgga cgacaaaggc gatgccggca agctcgcgcg ccagcacggc ctcacgcgca aggcgctgga agccgcgatc gccgcggtgc gcggcggctc gcaggtgcac agccaggatg cggaaagcca gcgcgaggcg ctgaagaaat acacggtcga cctgaccgag cgcgcccgcg cgggcaagct cgatccggtg atcggccgcg acgacgaaat ccgccgctcg atccagatcc tgcagcgccg caccaagaac aacccggtgc tgatcggcga gccgggcgtc ggcaagacgg cgatcgtcga agggctcgcg cagcggatcg tcaacggcga agtgcccgag acgctgaagg gcaagcgcgt gctgtcgctc gacatggccg cgctgctcgc cggcgcgaag tatcgcggcg agttcgagga gcgcctgaag gcggtgctca acgacatcgc gaaggacgaa ggccagacga tcgtcttcat cgacgagatc cacacgatgg tcggcgcggg caaggccgaa ggcgcgatgg acgcgggcaa catgctgaag ccggcgctgt cgcgcggcga gctgcactgc atcggcgcga ccacgctcga cgaataccgc aagtacatcg agaaggacgc cgcgctcgag cgccgcttcc agaaggtgct cgtcgacgag ccgagcgtcg aggcgacgat cgcgatcctg cgcgggctgc aggagaagta cgaactgcac cacggcgtcg acatcaccga cccggcgatc gtcgccgcgg ccgagctgtc gcaccgctac atcaccgacc gcttcctgcc cgacaaggcg atcgacctga tcgacgaagc cgcttcgaag atcaagatgg aaatcgattc gaagcccgaa gagatggaca agctcgaccg tcgtctgatt cagctgaaga tcgagcgcga agccgtgaag aaggagcagg acgaagcgtc gcagaagcgc ctgcagctga tcgaggaaga gatcgaacgc ctcggccgcg aatatgcgga cctggaagag atctggaccg ccgagaaggc cgcggtgcag ggcagcgcgc agctcaagga agagatcgag aaggtgcggg cggacatcgc gcgcctgcag cgtgaaggca agctcgagaa ggtcgccgag ctgcagtacg gcaagctgcc gcagctcgag tcgcaactga agcaggtcac gcaggccgaa gagcaggagc agcacaaccc gacgcgcccg cgcctgctgc gcacgcaggt cggcgccgag gaaatcgcgg aagtcgtgtc gcgttcgacg gggattcccg tgtcgcggat gatgcagggc gaacgcgaga agctgctgca catcgaggaa aagctgcatg agcgcgtggt cggccaggac gaggcgatca gcgcggtggc cgatgcgatc cgtcggtcgc gcgcgggtct cgccgatccg aaccgtccgt acggctcgtt cctgttcctc ggcccgacgg gggtcggcaa gaccgagctg tgcaaggcgc tggcgtcgtt cctgttcgat tcggaagagc acctgatccg catcgacatg agcgagttca tggagaagca cagcgtcgcg cggctgatcg gcgcgccgcc cggctatgtc ggctacgagg aaggcggcta cctgacggaa gccgtgcgcc gcaagccgta cagcgtgatc ctgctcgacg agatcgagaa ggcgaatccg gacgtgttca acgtgctgct gcaggtgctc gacgacggcc gcatgacgga cgggcagggg cgcacggtcg acttcaagaa cacggtcatc gtgatgacgt cgaatctcgg ctcgcaggtg atccaggcca tgaccggctc gccgcaggac gagatcaagg acgccgtgtg gctcgaggtg aagcagcatt tccgccccga gttcctgaac cggatcgacg acgtcgtcgt gttccatgcg ctcgaccgca gcaacatcga gtcgatcgcg aagatccagc tggcgcgcct gcacgaccgg cttgcgaagc tggacatggc gctcgacgtg tcgcccgctg cgctcgaaca gatcgcgaag gtcggctacg atccggtgtt cggtgcgcgg ccgctgaagc gcgcgatcca gcaggagatc gagaacccgg tcgcgaagct gatcctcgcc ggccgcttcg gtccgaagga cgtgatcccg gtcgacgtca acgacggcca gttcgtgttc gaccgcgtcg tgcactga
Details for BuceA.18032.a.A2.GE33226
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 68kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 43
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDEIRRSIQ ILQRRTKNNP VLIGEPGVGK TAIVEGLAQR IVNGEVPETL KGKRVLSLDM AALLAGAKYR GEFEERLKAV LNDIAKDEGQ TIVFIDEIHT MVGAGKAEGA MDAGNMLKPA LSRGELHCIG ATTLDEYRKY IEKDAALERR FQKVLVDEPS VEATIAILRG LQEKYELHHG VXITDPAIVA AAELSHRYIT DRFLPDKAID LIDEAASKIK MEIDSKPEEM DKLDRRLIQL KIEREP
Validated NT Sequence
ttnngtttan ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggacg aaatccgccg ctcgatccag atcctgcagc gccgcaccaa gaacaacccg gtgctgatcg gcgagccggg cgtcggcaag acggcgatcg tcgaagggct cgcgcagcgg atcgtcaacg gcgaagtgcc cgagacgctg aagggcaagc gcgtgctgtc gctcgacatg gccgcgctgc tcgccggcgc gaagtatcgc ggcgagttcg aggagcgcct gaaggcggtg ctcaacgaca tcgcgaagga cgaaggccag acgatcgtct tcatcgacga gatccacacg atggtcggcg cgggcaaggc cgaaggcgcg atggacgcgg gcaacatgct gaagccggcg ctgtcgcgcg gcgagctgca ctgcatcggc gcgaccacgc tcgacgaata ccgcaagtac atcgagaagg acgccgcgct cgagcgccgc ttccagaagg tgctcgtcga cgagccgagc gtcgaggcga cgatcgcgat cctgcgcggg ctgcaggaga agtacgaact gcaccacggc gtcnacatca ccgacccggc gatcgtcgcc gcggccgagc tgtcgcaccg ctacatcacc gaccgcttcc tgcccgacaa ggcgatcgac ctgatcgacg aagccgcttc naagatcaag atggaaatcg attcgaagcc cgaagagatg gacaagctcg accgtcgtct gattcagctg aagatcgagc gcgaaccntg aannanganc nnacgaancg tcncnnnagc gcctgcagct gatcgaggaa gannncgaac gnctcggncg cgaatatgcg gacctgnnan agatctggac cgccganann ncncnnngcn ngncnnncgc nntcaggaag anntcgagaa ngngnnggcg ganntcgcgc gccngcannn ngnangcaag ctcgnnnnnn nncnanntnn nntacgnnng ctgccncnnn cgagtccnca antgnancng nnnnnncnnn ngnannannn nnnnnnnnnn anccnanncn nnncnnnngn nnnnnnnnnn nnnnnnccnn nnnnnnnnnn annnnnnggg nnncnnnnnn nnnnnnnnnn cngnnnnnnn nnnnnnnnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDEIRRSIQ ILQRRTKNNP VLIGEPGVGK TAIVEGLAQR IVNGEVPETL KGKRVLSLDM AALLAGAKYR GEFEERLKAV LNDIAKDEGQ TIVFIDEIHT MVGAGKAEGA MDAGNMLKPA LSRGELHCIG ATTLDEYRKY IEKDAALERR FQKVLVDEPS VEATIAILRG LQEKYELHHG VDITDPAIVA AAELSHRYIT DRFLPDKAID LIDEAASKIK MEIDSKPEEM DKLDRRLIQL KIEREAVKKE QDEASQKRLQ LIEEEIERLG REYADLEEIW TAEKAAVQGS AQLKEEIEKV RADIARLQRE GKLEKVAELQ YGKLPQLESQ LKQVTQAEEQ EQHNPTRPRL LRTQVGAEEI AEVVSRSTGI PVSRMMQGER EKLLHIEEKL HERVVGQDEA ISAVADAIRR SRAGLADPNR PYGSFLFLGP TGVGKTELCK ALASFLFDSE EHLIRIDMSE FMEKHSVARL IGAPPGYVGY EEGGYLTEAV RRKPYSVILL DEIEKANPDV FNVLLQVLDD GRMTDGQGRT VDFKNTVIVM TSNLGSQVIQ AMTGSPQDEI KDAVWLEVKQ HFRPEFLNRI DDVVVFHALD RSN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgaaatc cgccgctcga tccagatcct gcagcgccgc accaagaaca acccggtgct gatcggcgag ccgggcgtcg gcaagacggc gatcgtcgaa gggctcgcgc agcggatcgt caacggcgaa gtgcccgaga cgctgaaggg caagcgcgtg ctgtcgctcg acatggccgc gctgctcgcc ggcgcgaagt atcgcggcga gttcgaggag cgcctgaagg cggtgctcaa cgacatcgcg aaggacgaag gccagacgat cgtcttcatc gacgagatcc acacgatggt cggcgcgggc aaggccgaag gcgcgatgga cgcgggcaac atgctgaagc cggcgctgtc gcgcggcgag ctgcactgca tcggcgcgac cacgctcgac gaataccgca agtacatcga gaaggacgcc gcgctcgagc gccgcttcca gaaggtgctc gtcgacgagc cgagcgtcga ggcgacgatc gcgatcctgc gcgggctgca ggagaagtac gaactgcacc acggcgtcga catcaccgac ccggcgatcg tcgccgcggc cgagctgtcg caccgctaca tcaccgaccg cttcctgccc gacaaggcga tcgacctgat cgacgaagcc gcttcgaaga tcaagatgga aatcgattcg aagcccgaag agatggacaa gctcgaccgt cgtctgattc agctgaagat cgagcgcgaa gccgtgaaga aggagcagga cgaagcgtcg cagaagcgcc tgcagctgat cgaggaagag atcgaacgcc tcggccgcga atatgcggac ctggaagaga tctggaccgc cgagaaggcc gcggtgcagg gcagcgcgca gctcaaggaa gagatcgaga aggtgcgggc ggacatcgcg cgcctgcagc gtgaaggcaa gctcgagaag gtcgccgagc tgcagtacgg caagctgccg cagctcgagt cgcaactgaa gcaggtcacg caggccgaag agcaggagca gcacaacccg acgcgcccgc gcctgctgcg cacgcaggtc ggcgccgagg aaatcgcgga agtcgtgtcg cgttcgacgg ggattcccgt gtcgcggatg atgcagggcg aacgcgagaa gctgctgcac atcgaggaaa agctgcatga gcgcgtggtc ggccaggacg aggcgatcag cgcggtggcc gatgcgatcc gtcggtcgcg cgcgggtctc gccgatccga accgtccgta cggctcgttc ctgttcctcg gcccgacggg ggtcggcaag accgagctgt gcaaggcgct ggcgtcgttc ctgttcgatt cggaagagca cctgatccgc atcgacatga gcgagttcat ggagaagcac agcgtcgcgc ggctgatcgg cgcgccgccc ggctatgtcg gctacgagga aggcggctac ctgacggaag ccgtgcgccg caagccgtac agcgtgatcc tgctcgacga gatcgagaag gcgaatccgg acgtgttcaa cgtgctgctg caggtgctcg acgacggccg catgacggac gggcaggggc gcacggtcga cttcaagaac acggtcatcg tgatgacgtc gaatctcggc tcgcaggtga tccaggccat gaccggctcg ccgcaggacg agatcaagga cgccgtgtgg ctcgaggtga agcagcattt ccgccccgag ttcctgaacc ggatcgacga cgtcgtcgtg ttccatgcgc tcgaccgcag caactaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BuceA.18032.a.A2.PS01284
PURIFICATION DATe: 11/30/2011
CONCENTRATION: 38.19mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 43
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDEIRRSIQ ILQRRTKNNP VLIGEPGVGK TAIVEGLAQR IVNGEVPETL KGKRVLSLDM AALLAGAKYR GEFEERLKAV LNDIAKDEGQ TIVFIDEIHT MVGAGKAEGA MDAGNMLKPA LSRGELHCIG ATTLDEYRKY IEKDAALERR FQKVLVDEPS VEATIAILRG LQEKYELHHG VXITDPAIVA AAELSHRYIT DRFLPDKAID LIDEAASKIK MEIDSKPEEM DKLDRRLIQL KIEREP
Validated NT Sequence
ttnngtttan ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggacg aaatccgccg ctcgatccag atcctgcagc gccgcaccaa gaacaacccg gtgctgatcg gcgagccggg cgtcggcaag acggcgatcg tcgaagggct cgcgcagcgg atcgtcaacg gcgaagtgcc cgagacgctg aagggcaagc gcgtgctgtc gctcgacatg gccgcgctgc tcgccggcgc gaagtatcgc ggcgagttcg aggagcgcct gaaggcggtg ctcaacgaca tcgcgaagga cgaaggccag acgatcgtct tcatcgacga gatccacacg atggtcggcg cgggcaaggc cgaaggcgcg atggacgcgg gcaacatgct gaagccggcg ctgtcgcgcg gcgagctgca ctgcatcggc gcgaccacgc tcgacgaata ccgcaagtac atcgagaagg acgccgcgct cgagcgccgc ttccagaagg tgctcgtcga cgagccgagc gtcgaggcga cgatcgcgat cctgcgcggg ctgcaggaga agtacgaact gcaccacggc gtcnacatca ccgacccggc gatcgtcgcc gcggccgagc tgtcgcaccg ctacatcacc gaccgcttcc tgcccgacaa ggcgatcgac ctgatcgacg aagccgcttc naagatcaag atggaaatcg attcgaagcc cgaagagatg gacaagctcg accgtcgtct gattcagctg aagatcgagc gcgaaccntg aannanganc nnacgaancg tcncnnnagc gcctgcagct gatcgaggaa gannncgaac gnctcggncg cgaatatgcg gacctgnnan agatctggac cgccganann ncncnnngcn ngncnnncgc nntcaggaag anntcgagaa ngngnnggcg ganntcgcgc gccngcannn ngnangcaag ctcgnnnnnn nncnanntnn nntacgnnng ctgccncnnn cgagtccnca antgnancng nnnnnncnnn ngnannannn nnnnnnnnnn anccnanncn nnncnnnngn nnnnnnnnnn nnnnnnccnn nnnnnnnnnn annnnnnggg nnncnnnnnn nnnnnnnnnn cngnnnnnnn nnnnnnnnaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDEIRRSIQ ILQRRTKNNP VLIGEPGVGK TAIVEGLAQR IVNGEVPETL KGKRVLSLDM AALLAGAKYR GEFEERLKAV LNDIAKDEGQ TIVFIDEIHT MVGAGKAEGA MDAGNMLKPA LSRGELHCIG ATTLDEYRKY IEKDAALERR FQKVLVDEPS VEATIAILRG LQEKYELHHG VDITDPAIVA AAELSHRYIT DRFLPDKAID LIDEAASKIK MEIDSKPEEM DKLDRRLIQL KIEREAVKKE QDEASQKRLQ LIEEEIERLG REYADLEEIW TAEKAAVQGS AQLKEEIEKV RADIARLQRE GKLEKVAELQ YGKLPQLESQ LKQVTQAEEQ EQHNPTRPRL LRTQVGAEEI AEVVSRSTGI PVSRMMQGER EKLLHIEEKL HERVVGQDEA ISAVADAIRR SRAGLADPNR PYGSFLFLGP TGVGKTELCK ALASFLFDSE EHLIRIDMSE FMEKHSVARL IGAPPGYVGY EEGGYLTEAV RRKPYSVILL DEIEKANPDV FNVLLQVLDD GRMTDGQGRT VDFKNTVIVM TSNLGSQVIQ AMTGSPQDEI KDAVWLEVKQ HFRPEFLNRI DDVVVFHALD RSN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACGAAATC CGCCGCTCGA TCCAGATCCT GCAGCGCCGC ACCAAGAACA ACCCGGTGCT GATCGGCGAG CCGGGCGTCG GCAAGACGGC GATCGTCGAA GGGCTCGCGC AGCGGATCGT CAACGGCGAA GTGCCCGAGA CGCTGAAGGG CAAGCGCGTG CTGTCGCTCG ACATGGCCGC GCTGCTCGCC GGCGCGAAGT ATCGCGGCGA GTTCGAGGAG CGCCTGAAGG CGGTGCTCAA CGACATCGCG AAGGACGAAG GCCAGACGAT CGTCTTCATC GACGAGATCC ACACGATGGT CGGCGCGGGC AAGGCCGAAG GCGCGATGGA CGCGGGCAAC ATGCTGAAGC CGGCGCTGTC GCGCGGCGAG CTGCACTGCA TCGGCGCGAC CACGCTCGAC GAATACCGCA AGTACATCGA GAAGGACGCC GCGCTCGAGC GCCGCTTCCA GAAGGTGCTC GTCGACGAGC CGAGCGTCGA GGCGACGATC GCGATCCTGC GCGGGCTGCA GGAGAAGTAC GAACTGCACC ACGGCGTCGA CATCACCGAC CCGGCGATCG TCGCCGCGGC CGAGCTGTCG CACCGCTACA TCACCGACCG CTTCCTGCCC GACAAGGCGA TCGACCTGAT CGACGAAGCC GCTTCGAAGA TCAAGATGGA AATCGATTCG AAGCCCGAAG AGATGGACAA GCTCGACCGT CGTCTGATTC AGCTGAAGAT CGAGCGCGAA GCCGTGAAGA AGGAGCAGGA CGAAGCGTCG CAGAAGCGCC TGCAGCTGAT CGAGGAAGAG ATCGAACGCC TCGGCCGCGA ATATGCGGAC CTGGAAGAGA TCTGGACCGC CGAGAAGGCC GCGGTGCAGG GCAGCGCGCA GCTCAAGGAA GAGATCGAGA AGGTGCGGGC GGACATCGCG CGCCTGCAGC GTGAAGGCAA GCTCGAGAAG GTCGCCGAGC TGCAGTACGG CAAGCTGCCG CAGCTCGAGT CGCAACTGAA GCAGGTCACG CAGGCCGAAG AGCAGGAGCA GCACAACCCG ACGCGCCCGC GCCTGCTGCG CACGCAGGTC GGCGCCGAGG AAATCGCGGA AGTCGTGTCG CGTTCGACGG GGATTCCCGT GTCGCGGATG ATGCAGGGCG AACGCGAGAA GCTGCTGCAC ATCGAGGAAA AGCTGCATGA GCGCGTGGTC GGCCAGGACG AGGCGATCAG CGCGGTGGCC GATGCGATCC GTCGGTCGCG CGCGGGTCTC GCCGATCCGA ACCGTCCGTA CGGCTCGTTC CTGTTCCTCG GCCCGACGGG GGTCGGCAAG ACCGAGCTGT GCAAGGCGCT GGCGTCGTTC CTGTTCGATT CGGAAGAGCA CCTGATCCGC ATCGACATGA GCGAGTTCAT GGAGAAGCAC AGCGTCGCGC GGCTGATCGG CGCGCCGCCC GGCTATGTCG GCTACGAGGA AGGCGGCTAC CTGACGGAAG CCGTGCGCCG CAAGCCGTAC AGCGTGATCC TGCTCGACGA GATCGAGAAG GCGAATCCGG ACGTGTTCAA CGTGCTGCTG CAGGTGCTCG ACGACGGCCG CATGACGGAC GGGCAGGGGC GCACGGTCGA CTTCAAGAAC ACGGTCATCG TGATGACGTC GAATCTCGGC TCGCAGGTGA TCCAGGCCAT GACCGGCTCG CCGCAGGACG AGATCAAGGA CGCCGTGTGG CTCGAGGTGA AGCAGCATTT CCGCCCCGAG TTCCTGAACC GGATCGACGA CGTCGTCGTG TTCCATGCGC TCGACCGCAG CAACTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA