BuceA.18021.a
Putative uncharacterized protein
CENTER ID:
BuceA.18021.a
ORGANISM:
Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
3
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
BuceA.18021.a.A1.GE33224
full length
info
1
369
BuceA.18021.a.A1.GE33224
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
BuceA.18021.a.A1.PD00490
full length
info
1
369
PD00490
BuceA.18021.a.A1.PW33834
full length
info
1
369
PW33834
Structures
6ANS
DEPOSITED: 8/14/2017
DETERMINATION: XRay
CLONE: BuceA.18021.a.A1.GE33224
PROTEIN: BuceA.18021.a.A1.PD00490
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDDTRATQL LSGQTWADFC DTLKRSGEQI LRTDAPDDPL TRAEGFRYLS RLMRIALEMH VEFADGAWPG FFSPSHETAK IGADNPDNLY QYARVDGRCE YRVTGRRGTV AYLSFGTQKG GYETDGKMLQ TGFLDAKQLE IAPDGSVEIV LSATPRAGNW VRMEPDTNAL LVRQTFLDRR TETPAQLKIE RIDAQARPAP LDPLALQGGL MRAAQFVEQT SKLFADWAAS YRPHVNALPP ADQALCQSVG GDPNIYYYHS CWSLAADEAL VIDVDTVPDC DFWNVQLNNY WMESLDYRHF DICVNKHSAR PNADGGVTVI VAATRPGSAN WLDTAGHRTG TICWRWVGAA QPVHPRTRVV KLAALKEAA
NT Sequence
atgacggatg acacacgcgc cacgcaattg ctttcgggcc agacctgggc cgatttctgc gacaccctga aacgcagcgg agaacagatc ctgcgcaccg acgcgccgga cgatccgctc acgcgtgcgg aaggcttccg ctacctgagc cgactgatgc gcatcgcgct ggaaatgcac gtcgagttcg cggacggcgc gtggccgggc ttcttctcgc cgtcccacga gaccgcgaag atcggcgccg acaaccccga caacctgtac cagtacgcgc gcgtcgacgg ccgctgcgaa taccgcgtga cgggacggcg cggcacggtc gcatacctga gcttcggcac acagaagggc ggctacgaga ccgacggcaa gatgctgcag acaggctttc tcgacgcgaa gcagctcgag atcgcgccgg acggcagcgt cgagatcgtg ttgagcgcga cgccgcgcgc gggcaactgg gtgcggatgg agccggacac caacgcgctg ctggtgcgcc agaccttcct cgaccggcgc acggagacgc ccgcgcaact gaagatcgaa cgcatcgatg cgcaggcgcg ccccgcgccg ctcgatccgc tcgcgctgca gggcggcctg atgcgcgccg cgcagttcgt cgagcagacg tcgaagctgt tcgcggactg ggccgcgagc tatcgcccgc acgtgaacgc gctgccgccc gccgaccagg cgctgtgcca gtcggtcggc ggcgatccga acatctacta ctaccactcg tgctggtcgc tcgccgccga cgaggcgctc gtgatcgacg tcgataccgt gccggactgc gatttctgga acgtgcagct caacaactac tggatggagt cgctcgacta tcggcacttc gacatctgcg tgaacaagca cagcgctcgg cccaatgcgg acggcggggt gacggtgatc gtcgcggcca cgcggccggg cagcgcgaac tggctcgata ccgccgggca tcgcaccggc acgatctgct ggcgctgggt gggcgccgcg caaccggtgc atccgcgcac gcgcgtcgtg aagctcgccg cgctgaagga ggccgcatga
Details for BuceA.18021.a.A1.GE33224
HARVESTED ON:
8/30/2011
SEQUENCED ON:
1/10/2017
EXPECTED MW:
43kDa
OBSERVED MW:
46kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
100
PERCENT COVERAGE:
97
Details for BuceA.18021.a.A1.PD00490
PURIFICATION DATe:
4/5/2017
CONCENTRATION:
15.21mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25mM tris pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
10
VIAL VOLUME:
100µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
97
Details for BuceA.18021.a.A1.PW33834
PURIFICATION DATe:
11/19/2011
CONCENTRATION:
40.37mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
15
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
97