BuceA.18021.a
Putative uncharacterized protein
| CENTER ID: | BuceA.18021.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.18021.a.A1.GE33224 | full length | 1 | 369 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.18021.a.A1.PD00490 | full length | 1 | 369 | ||
| BuceA.18021.a.A1.PW33834 | full length | 1 | 369 |
Structures
6ANS
DEPOSITED: 8/14/2017
DETERMINATION: XRay
CLONE: BuceA.18021.a.A1.GE33224
PROTEIN: BuceA.18021.a.A1.PD00490
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_169922 |
| PATRIC ID: | fig|216591.5.peg.2356 |
| RefSeq: | YP_002231384.1 |
| UniProt: | B4EDY7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDDTRATQL LSGQTWADFC DTLKRSGEQI LRTDAPDDPL TRAEGFRYLS RLMRIALEMH VEFADGAWPG FFSPSHETAK IGADNPDNLY QYARVDGRCE YRVTGRRGTV AYLSFGTQKG GYETDGKMLQ TGFLDAKQLE IAPDGSVEIV LSATPRAGNW VRMEPDTNAL LVRQTFLDRR TETPAQLKIE RIDAQARPAP LDPLALQGGL MRAAQFVEQT SKLFADWAAS YRPHVNALPP ADQALCQSVG GDPNIYYYHS CWSLAADEAL VIDVDTVPDC DFWNVQLNNY WMESLDYRHF DICVNKHSAR PNADGGVTVI VAATRPGSAN WLDTAGHRTG TICWRWVGAA QPVHPRTRVV KLAALKEAA
NT Sequence
atgacggatg acacacgcgc cacgcaattg ctttcgggcc agacctgggc cgatttctgc gacaccctga aacgcagcgg agaacagatc ctgcgcaccg acgcgccgga cgatccgctc acgcgtgcgg aaggcttccg ctacctgagc cgactgatgc gcatcgcgct ggaaatgcac gtcgagttcg cggacggcgc gtggccgggc ttcttctcgc cgtcccacga gaccgcgaag atcggcgccg acaaccccga caacctgtac cagtacgcgc gcgtcgacgg ccgctgcgaa taccgcgtga cgggacggcg cggcacggtc gcatacctga gcttcggcac acagaagggc ggctacgaga ccgacggcaa gatgctgcag acaggctttc tcgacgcgaa gcagctcgag atcgcgccgg acggcagcgt cgagatcgtg ttgagcgcga cgccgcgcgc gggcaactgg gtgcggatgg agccggacac caacgcgctg ctggtgcgcc agaccttcct cgaccggcgc acggagacgc ccgcgcaact gaagatcgaa cgcatcgatg cgcaggcgcg ccccgcgccg ctcgatccgc tcgcgctgca gggcggcctg atgcgcgccg cgcagttcgt cgagcagacg tcgaagctgt tcgcggactg ggccgcgagc tatcgcccgc acgtgaacgc gctgccgccc gccgaccagg cgctgtgcca gtcggtcggc ggcgatccga acatctacta ctaccactcg tgctggtcgc tcgccgccga cgaggcgctc gtgatcgacg tcgataccgt gccggactgc gatttctgga acgtgcagct caacaactac tggatggagt cgctcgacta tcggcacttc gacatctgcg tgaacaagca cagcgctcgg cccaatgcgg acggcggggt gacggtgatc gtcgcggcca cgcggccggg cagcgcgaac tggctcgata ccgccgggca tcgcaccggc acgatctgct ggcgctgggt gggcgccgcg caaccggtgc atccgcgcac gcgcgtcgtg aagctcgccg cgctgaagga ggccgcatga
Details for BuceA.18021.a.A1.GE33224
| HARVESTED ON: | 8/30/2011 |
| SEQUENCED ON: | 1/10/2017 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 46kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMTDDTRA TQLLSGQTWA DFCDTLKRSG EQILRTDAPD DPLTRAEGFR YLSRLMRIAL EMHVEFADGA WPGFFSPSHE TAKIGADNPD NLYQYARVDG RCEYRVTGRR GTVAYLSFGT QKGGYETDGK MLQTGFLDAK QLEIAPDGSV EIVLSATPRA GNWVRMEPDT NALLVRQTFL DRRTETPAQL KIERIDAQAR PAPLDPLALQ GGLMRAAQFV EQTSKLFADW AASYRPHVNA LPPADQALCQ SVGGDPNIYY YHSCWSLAAD EALVIDVDTV PDCDFWNVQL NNYWMESLDY RHFDICVNKH SARPNADGGV TVIVAATRPG SANWLDTAGH RTGTICWRWV GAAQPVHPRT RVVKLAALKE AA
Validated NT Sequence
ttattatgcg gcctccttca gcgcggcgag cttcacgacg cgcgtgcgcg gatgcaccgg ttgcgcggcg cccacccagc gccagcagat cgtgccggtg cgatgcccgg cggtatcgag ccagttcgcg ctgcccggcc gcgtggccgc gacgatcacc gtcaccccgc cgtccgcatt gggccgagcg ctgtgcttgt tcacgcagat gtcgaagtgc cgatagtcga gcgactccat ccagtagttg ttgagctgca cgttccagaa atcgcagtcc ggcacggtat cgacgtcgat cacgagcgcc tcgtcggcgg cgagcgacca gcacgagtgg tagtagtaga tgttcggatc gccgccgacc gactggcaca gcgcctggtc ggcgggcggc agcgcgttca cgtgcgggcg atagctcgcg gcccagtccg cgaacagctt cgacgtctgc tcgacgaact gcgcggcgcg catcaggccg ccctgcagcg cgagcggatc gagcggcgcg gggcgcgcct gcgcatcgat gcgttcgatc ttcagttgcg cgggcgtctc cgtgcgccgg tcgaggaagg tctggcgcac cagcagcgcg ttggtgtccg gctccatccg cacccagttg cccgcgcgcg gcgtcgcgct caacacgatc tcgacgctgc cgtccggcgc gatctcgagc tgcttcgcgt cgagaaagcc tgtctgcagc atcttgccgt cggtctcgta gccgcccttc tgtgtgccga agctcaggta tgcgaccgtg ccgcgccgtc ccgtcacgcg gtattcgcag cggccgtcga cgcgcgcgta ctggtacagg ttgtcggggt tgtcggcgcc gatcttcgcg gtctcgtggg acggcgagaa gaagcccggc cacgcgccgt ccgcgaactc gacgtgcatt tccagcgcga tgcgcatcag tcggctcagg tagcggaagc cttccgcacg cgtgagcgga tcgtccggcg cgtcggtgcg caggatctgt tctccgctgc gtttcagggt gtcgcagaaa tcggcccagg tctggcccga aagcaattgc gtggcgcgtg tgtcatccgt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDDTRATQ LLSGQTWADF CDTLKRSGEQ ILRTDAPDDP LTRAEGFRYL SRLMRIALEM HVEFADGAWP GFFSPSHETA KIGADNPDNL YQYARVDGRC EYRVTGRRGT VAYLSFGTQK GGYETDGKML QTGFLDAKQL EIAPDGSVEI VLSATPRAGN WVRMEPDTNA LLVRQTFLDR RTETPAQLKI ERIDAQARPA PLDPLALQGG LMRAAQFVEQ TSKLFADWAA SYRPHVNALP PADQALCQSV GGDPNIYYYH SCWSLAADEA LVIDVDTVPD CDFWNVQLNN YWMESLDYRH FDICVNKHSA RPNADGGVTV IVAATRPGSA NWLDTAGHRT GTICWRWVGA AQPVHPRTRV VKLAALKEAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggatgac acacgcgcca cgcaattgct ttcgggccag acctgggccg atttctgcga caccctgaaa cgcagcggag aacagatcct gcgcaccgac gcgccggacg atccgctcac gcgtgcggaa ggcttccgct acctgagccg actgatgcgc atcgcgctgg aaatgcacgt cgagttcgcg gacggcgcgt ggccgggctt cttctcgccg tcccacgaga ccgcgaagat cggcgccgac aaccccgaca acctgtacca gtacgcgcgc gtcgacggcc gctgcgaata ccgcgtgacg ggacggcgcg gcacggtcgc atacctgagc ttcggcacac agaagggcgg ctacgagacc gacggcaaga tgctgcagac aggctttctc gacgcgaagc agctcgagat cgcgccggac ggcagcgtcg agatcgtgtt gagcgcgacg ccgcgcgcgg gcaactgggt gcggatggag ccggacacca acgcgctgct ggtgcgccag accttcctcg accggcgcac ggagacgccc gcgcaactga agatcgaacg catcgatgcg caggcgcgcc ccgcgccgct cgatccgctc gcgctgcagg gcggcctgat gcgcgccgcg cagttcgtcg agcagacgtc gaagctgttc gcggactggg ccgcgagcta tcgcccgcac gtgaacgcgc tgccgcccgc cgaccaggcg ctgtgccagt cggtcggcgg cgatccgaac atctactact accactcgtg ctggtcgctc gccgccgacg aggcgctcgt gatcgacgtc gataccgtgc cggactgcga tttctggaac gtgcagctca acaactactg gatggagtcg ctcgactatc ggcacttcga catctgcgtg aacaagcaca gcgctcggcc caatgcggac ggcggggtga cggtgatcgt cgcggccacg cggccgggca gcgcgaactg gctcgatacc gccgggcatc gcaccggcac gatctgctgg cgctgggtgg gcgccgcgca accggtgcat ccgcgcacgc gcgtcgtgaa gctcgccgcg ctgaaggagg ccgcataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BuceA.18021.a.A1.PD00490
| PURIFICATION DATe: | 4/5/2017 |
| CONCENTRATION: | 15.21mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25mM tris pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTDDTRA TQLLSGQTWA DFCDTLKRSG EQILRTDAPD DPLTRAEGFR YLSRLMRIAL EMHVEFADGA WPGFFSPSHE TAKIGADNPD NLYQYARVDG RCEYRVTGRR GTVAYLSFGT QKGGYETDGK MLQTGFLDAK QLEIAPDGSV EIVLSATPRA GNWVRMEPDT NALLVRQTFL DRRTETPAQL KIERIDAQAR PAPLDPLALQ GGLMRAAQFV EQTSKLFADW AASYRPHVNA LPPADQALCQ SVGGDPNIYY YHSCWSLAAD EALVIDVDTV PDCDFWNVQL NNYWMESLDY RHFDICVNKH SARPNADGGV TVIVAATRPG SANWLDTAGH RTGTICWRWV GAAQPVHPRT RVVKLAALKE AA
Validated NT Sequence
ttattatgcg gcctccttca gcgcggcgag cttcacgacg cgcgtgcgcg gatgcaccgg ttgcgcggcg cccacccagc gccagcagat cgtgccggtg cgatgcccgg cggtatcgag ccagttcgcg ctgcccggcc gcgtggccgc gacgatcacc gtcaccccgc cgtccgcatt gggccgagcg ctgtgcttgt tcacgcagat gtcgaagtgc cgatagtcga gcgactccat ccagtagttg ttgagctgca cgttccagaa atcgcagtcc ggcacggtat cgacgtcgat cacgagcgcc tcgtcggcgg cgagcgacca gcacgagtgg tagtagtaga tgttcggatc gccgccgacc gactggcaca gcgcctggtc ggcgggcggc agcgcgttca cgtgcgggcg atagctcgcg gcccagtccg cgaacagctt cgacgtctgc tcgacgaact gcgcggcgcg catcaggccg ccctgcagcg cgagcggatc gagcggcgcg gggcgcgcct gcgcatcgat gcgttcgatc ttcagttgcg cgggcgtctc cgtgcgccgg tcgaggaagg tctggcgcac cagcagcgcg ttggtgtccg gctccatccg cacccagttg cccgcgcgcg gcgtcgcgct caacacgatc tcgacgctgc cgtccggcgc gatctcgagc tgcttcgcgt cgagaaagcc tgtctgcagc atcttgccgt cggtctcgta gccgcccttc tgtgtgccga agctcaggta tgcgaccgtg ccgcgccgtc ccgtcacgcg gtattcgcag cggccgtcga cgcgcgcgta ctggtacagg ttgtcggggt tgtcggcgcc gatcttcgcg gtctcgtggg acggcgagaa gaagcccggc cacgcgccgt ccgcgaactc gacgtgcatt tccagcgcga tgcgcatcag tcggctcagg tagcggaagc cttccgcacg cgtgagcgga tcgtccggcg cgtcggtgcg caggatctgt tctccgctgc gtttcagggt gtcgcagaaa tcggcccagg tctggcccga aagcaattgc gtggcgcgtg tgtcatccgt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDDTRATQ LLSGQTWADF CDTLKRSGEQ ILRTDAPDDP LTRAEGFRYL SRLMRIALEM HVEFADGAWP GFFSPSHETA KIGADNPDNL YQYARVDGRC EYRVTGRRGT VAYLSFGTQK GGYETDGKML QTGFLDAKQL EIAPDGSVEI VLSATPRAGN WVRMEPDTNA LLVRQTFLDR RTETPAQLKI ERIDAQARPA PLDPLALQGG LMRAAQFVEQ TSKLFADWAA SYRPHVNALP PADQALCQSV GGDPNIYYYH SCWSLAADEA LVIDVDTVPD CDFWNVQLNN YWMESLDYRH FDICVNKHSA RPNADGGVTV IVAATRPGSA NWLDTAGHRT GTICWRWVGA AQPVHPRTRV VKLAALKEAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGATGAC ACACGCGCCA CGCAATTGCT TTCGGGCCAG ACCTGGGCCG ATTTCTGCGA CACCCTGAAA CGCAGCGGAG AACAGATCCT GCGCACCGAC GCGCCGGACG ATCCGCTCAC GCGTGCGGAA GGCTTCCGCT ACCTGAGCCG ACTGATGCGC ATCGCGCTGG AAATGCACGT CGAGTTCGCG GACGGCGCGT GGCCGGGCTT CTTCTCGCCG TCCCACGAGA CCGCGAAGAT CGGCGCCGAC AACCCCGACA ACCTGTACCA GTACGCGCGC GTCGACGGCC GCTGCGAATA CCGCGTGACG GGACGGCGCG GCACGGTCGC ATACCTGAGC TTCGGCACAC AGAAGGGCGG CTACGAGACC GACGGCAAGA TGCTGCAGAC AGGCTTTCTC GACGCGAAGC AGCTCGAGAT CGCGCCGGAC GGCAGCGTCG AGATCGTGTT GAGCGCGACG CCGCGCGCGG GCAACTGGGT GCGGATGGAG CCGGACACCA ACGCGCTGCT GGTGCGCCAG ACCTTCCTCG ACCGGCGCAC GGAGACGCCC GCGCAACTGA AGATCGAACG CATCGATGCG CAGGCGCGCC CCGCGCCGCT CGATCCGCTC GCGCTGCAGG GCGGCCTGAT GCGCGCCGCG CAGTTCGTCG AGCAGACGTC GAAGCTGTTC GCGGACTGGG CCGCGAGCTA TCGCCCGCAC GTGAACGCGC TGCCGCCCGC CGACCAGGCG CTGTGCCAGT CGGTCGGCGG CGATCCGAAC ATCTACTACT ACCACTCGTG CTGGTCGCTC GCCGCCGACG AGGCGCTCGT GATCGACGTC GATACCGTGC CGGACTGCGA TTTCTGGAAC GTGCAGCTCA ACAACTACTG GATGGAGTCG CTCGACTATC GGCACTTCGA CATCTGCGTG AACAAGCACA GCGCTCGGCC CAATGCGGAC GGCGGGGTGA CGGTGATCGT CGCGGCCACG CGGCCGGGCA GCGCGAACTG GCTCGATACC GCCGGGCATC GCACCGGCAC GATCTGCTGG CGCTGGGTGG GCGCCGCGCA ACCGGTGCAT CCGCGCACGC GCGTCGTGAA GCTCGCCGCG CTGAAGGAGG CCGCATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for BuceA.18021.a.A1.PW33834
| PURIFICATION DATe: | 11/19/2011 |
| CONCENTRATION: | 40.37mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTDDTRA TQLLSGQTWA DFCDTLKRSG EQILRTDAPD DPLTRAEGFR YLSRLMRIAL EMHVEFADGA WPGFFSPSHE TAKIGADNPD NLYQYARVDG RCEYRVTGRR GTVAYLSFGT QKGGYETDGK MLQTGFLDAK QLEIAPDGSV EIVLSATPRA GNWVRMEPDT NALLVRQTFL DRRTETPAQL KIERIDAQAR PAPLDPLALQ GGLMRAAQFV EQTSKLFADW AASYRPHVNA LPPADQALCQ SVGGDPNIYY YHSCWSLAAD EALVIDVDTV PDCDFWNVQL NNYWMESLDY RHFDICVNKH SARPNADGGV TVIVAATRPG SANWLDTAGH RTGTICWRWV GAAQPVHPRT RVVKLAALKE AA
Validated NT Sequence
ttattatgcg gcctccttca gcgcggcgag cttcacgacg cgcgtgcgcg gatgcaccgg ttgcgcggcg cccacccagc gccagcagat cgtgccggtg cgatgcccgg cggtatcgag ccagttcgcg ctgcccggcc gcgtggccgc gacgatcacc gtcaccccgc cgtccgcatt gggccgagcg ctgtgcttgt tcacgcagat gtcgaagtgc cgatagtcga gcgactccat ccagtagttg ttgagctgca cgttccagaa atcgcagtcc ggcacggtat cgacgtcgat cacgagcgcc tcgtcggcgg cgagcgacca gcacgagtgg tagtagtaga tgttcggatc gccgccgacc gactggcaca gcgcctggtc ggcgggcggc agcgcgttca cgtgcgggcg atagctcgcg gcccagtccg cgaacagctt cgacgtctgc tcgacgaact gcgcggcgcg catcaggccg ccctgcagcg cgagcggatc gagcggcgcg gggcgcgcct gcgcatcgat gcgttcgatc ttcagttgcg cgggcgtctc cgtgcgccgg tcgaggaagg tctggcgcac cagcagcgcg ttggtgtccg gctccatccg cacccagttg cccgcgcgcg gcgtcgcgct caacacgatc tcgacgctgc cgtccggcgc gatctcgagc tgcttcgcgt cgagaaagcc tgtctgcagc atcttgccgt cggtctcgta gccgcccttc tgtgtgccga agctcaggta tgcgaccgtg ccgcgccgtc ccgtcacgcg gtattcgcag cggccgtcga cgcgcgcgta ctggtacagg ttgtcggggt tgtcggcgcc gatcttcgcg gtctcgtggg acggcgagaa gaagcccggc cacgcgccgt ccgcgaactc gacgtgcatt tccagcgcga tgcgcatcag tcggctcagg tagcggaagc cttccgcacg cgtgagcgga tcgtccggcg cgtcggtgcg caggatctgt tctccgctgc gtttcagggt gtcgcagaaa tcggcccagg tctggcccga aagcaattgc gtggcgcgtg tgtcatccgt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDDTRATQ LLSGQTWADF CDTLKRSGEQ ILRTDAPDDP LTRAEGFRYL SRLMRIALEM HVEFADGAWP GFFSPSHETA KIGADNPDNL YQYARVDGRC EYRVTGRRGT VAYLSFGTQK GGYETDGKML QTGFLDAKQL EIAPDGSVEI VLSATPRAGN WVRMEPDTNA LLVRQTFLDR RTETPAQLKI ERIDAQARPA PLDPLALQGG LMRAAQFVEQ TSKLFADWAA SYRPHVNALP PADQALCQSV GGDPNIYYYH SCWSLAADEA LVIDVDTVPD CDFWNVQLNN YWMESLDYRH FDICVNKHSA RPNADGGVTV IVAATRPGSA NWLDTAGHRT GTICWRWVGA AQPVHPRTRV VKLAALKEAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGATGAC ACACGCGCCA CGCAATTGCT TTCGGGCCAG ACCTGGGCCG ATTTCTGCGA CACCCTGAAA CGCAGCGGAG AACAGATCCT GCGCACCGAC GCGCCGGACG ATCCGCTCAC GCGTGCGGAA GGCTTCCGCT ACCTGAGCCG ACTGATGCGC ATCGCGCTGG AAATGCACGT CGAGTTCGCG GACGGCGCGT GGCCGGGCTT CTTCTCGCCG TCCCACGAGA CCGCGAAGAT CGGCGCCGAC AACCCCGACA ACCTGTACCA GTACGCGCGC GTCGACGGCC GCTGCGAATA CCGCGTGACG GGACGGCGCG GCACGGTCGC ATACCTGAGC TTCGGCACAC AGAAGGGCGG CTACGAGACC GACGGCAAGA TGCTGCAGAC AGGCTTTCTC GACGCGAAGC AGCTCGAGAT CGCGCCGGAC GGCAGCGTCG AGATCGTGTT GAGCGCGACG CCGCGCGCGG GCAACTGGGT GCGGATGGAG CCGGACACCA ACGCGCTGCT GGTGCGCCAG ACCTTCCTCG ACCGGCGCAC GGAGACGCCC GCGCAACTGA AGATCGAACG CATCGATGCG CAGGCGCGCC CCGCGCCGCT CGATCCGCTC GCGCTGCAGG GCGGCCTGAT GCGCGCCGCG CAGTTCGTCG AGCAGACGTC GAAGCTGTTC GCGGACTGGG CCGCGAGCTA TCGCCCGCAC GTGAACGCGC TGCCGCCCGC CGACCAGGCG CTGTGCCAGT CGGTCGGCGG CGATCCGAAC ATCTACTACT ACCACTCGTG CTGGTCGCTC GCCGCCGACG AGGCGCTCGT GATCGACGTC GATACCGTGC CGGACTGCGA TTTCTGGAAC GTGCAGCTCA ACAACTACTG GATGGAGTCG CTCGACTATC GGCACTTCGA CATCTGCGTG AACAAGCACA GCGCTCGGCC CAATGCGGAC GGCGGGGTGA CGGTGATCGT CGCGGCCACG CGGCCGGGCA GCGCGAACTG GCTCGATACC GCCGGGCATC GCACCGGCAC GATCTGCTGG CGCTGGGTGG GCGCCGCGCA ACCGGTGCAT CCGCGCACGC GCGTCGTGAA GCTCGCCGCG CTGAAGGAGG CCGCATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA