BuceA.18020.a

Putative oxidoreductase

CENTER ID: BuceA.18020.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18020.a.A1.GE33223 full length 1 476
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18020.a.A1.PS01276 full length 1 476

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.60
RefSeq: YP_002229216.1
UniProt: B4EF01

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPTPNPTVAG RAPDGRPLPD DAVDAVLAAE LGDALVTRAA DIDPRYFTAY NEPAGVRPRA LVRPRSVDDV SRALALCMRL GQPVVPQGGL TGLARGAVAL GGEVVLSMER FAGIEALDAA AGTITVRAGT PLQTVQDAAE AAGFTFGVDL GARGSCQIGG MLATNAGGTR AIRYGMMREQ VLGLEAVLAD GTVVSSMNRM LKNNAGYDLK QLFIGSEGTL GVVTRAVLRL HPLLAAPATA LCRVRDYDAV VALWNRVRTL PEVVSFEAMW PAFYDYVARH TPGVSAPFVG DGGFVVLIEC ATSAPGGDAH HALETALAAG FDAALVDDAV LATSERQARD LWTLREGLAI DALPHLVNFD VSLPTGELGA FAERCEAVLR TRWPALTCLF FGHVGDGNVH IGVSLAAMTD ADADALDHCV YAVVRDMGGS VSAEHGIGVL KRPYLAHTRS DAEIGLMRRL KAALDPRGIL NPGKVL
NT Sequence
atgccgaccc cgaacccgac cgtcgcgggc cgcgcaccgg acggcaggcc gttgccggac gacgccgtcg atgcggtgct cgccgccgaa ctgggcgacg cgctcgtcac gcgcgcggcc gatatcgatc cacgttactt caccgcgtac aacgagccgg ccggcgtgcg gccgcgtgcg ctggtgcgcc cgcgcagcgt cgacgacgtg tcgcgcgcgc tcgcgctgtg catgcggctc ggccagccgg tcgtgccgca aggcgggctg accgggctcg cgcgcggggc ggtcgcgctg ggcggcgagg tcgtgctgtc gatggagcgc ttcgcgggca tcgaagcgct cgacgcggcg gccggcacga tcaccgtgcg cgcgggcacg ccgctgcaga ccgtgcagga cgccgcggaa gcggcgggct tcacgttcgg cgtcgatctc ggcgcgcgcg gctcgtgcca gatcggcggc atgctcgcga ccaacgcggg cggcacgcgt gcgatccgct acggaatgat gcgcgagcag gtgttggggc tcgaagcggt gctcgcggac ggcacggtcg tgtcgtcgat gaaccggatg ctgaagaaca acgcgggcta cgacctgaag cagctgttca tcggcagcga agggacgctc ggcgtcgtca cgcgcgcggt gctgcggctg catccgttgc tcgcggcgcc ggccacggcg ctctgccgcg tgcgcgacta cgacgcggtg gtcgcgctgt ggaaccgcgt gcgcacgctg ccggaagtcg tcagcttcga ggcgatgtgg ccggcgttct acgattacgt cgcgcggcat acgcccggtg tgtcggcacc gttcgtcggc gacggcggtt tcgtggtgtt gatcgaatgc gcgacgagcg cgccgggcgg cgatgcgcat catgcgctcg aaacggcgct cgccgcgggg ttcgatgcag cgctcgtcga cgatgcggta ctcgcgactt ccgagcggca ggcgcgcgat ttgtggacgc tgcgcgaagg cctcgcgatc gatgcgctgc cccatctcgt caacttcgac gtgagtctgc cgaccggcga actcggcgcg ttcgccgagc gctgcgaggc ggtgttgcgg acgcgctggc cggcgctcac gtgcctgttc ttcggccatg tcggcgacgg caacgtgcat atcggcgtgt cgctggccgc gatgaccgac gcggacgccg atgcgctcga tcactgcgtg tatgcggtgg tgcgcgacat gggcgggtcg gtgtcggccg agcacgggat cggcgtgctc aagcgtcctt atctcgcgca cacgcgcagc gacgcggaaa tcgggctgat gcgacgcctg aaggccgcgc tggacccgcg cggcatcctc aatccgggca aggtcctttg a
Details for BuceA.18020.a.A1.GE33223
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MPTPNPTVAG RAPDGRPLPD DAVDAVLAAE LGDALVTRAA DIDPRYFTAY NEPAGVRPRA LVRPRSVDDV SRALALCMRL GQPVVPQGGL TGLARGAVAL GGEVVLSMER FAGIEALDAA AGTITVRAGT PLQTVQDAAE AAGFTFGVDL GARGSCQIGG MLATNAGGTR AIRYGMMREQ VLGLEAVLAD GTVVSSMNRM LKNNAGYDLK QLFIGSEGTL GVVTRAVLRL HPLLAAPATA LCRVRDYDAV VALWNRVRTL PEVVSFEAMW PAFYDYVARH TPGVSAPFVG DGGFVVLIEC ATSAPGGDAH HALETALAAG FDAALVDDAV LATSERQARD LWTLREGLAI DALPHLVNFD VSLPTGELGA FAERCEAVLR TRWPALTCLF FGHVGDGNVH IGVSLAAMTD ADADALDHCV YAVVRDMGGS VSAEHGIGVL KRPYLAHTRS DAEIGLMRRL K
Validated NT Sequence
gccttcaggc gtcgcatcag cccgatttcc gcgtcgctgc gcgtgtgcgc gagataagga cgcttgagca cgccgatccc gtgctcggcc gacaccgacc cgcccatgtc gcgcaccacc gcatacacgc agtgatcgag cgcatcggcg tccgcgtcgg tcatcgcggc cagcgacacg ccgatatgca cgttgccgtc gccgacatgg ccgaagaaca ggcacgtgag cgccggccag cgcgtccgca acaccgcctc gcagcgctcg gcgaacgcgc cgagttcgcc ggtcggcaga ctcacgtcga agttgacgag atggggcagc gcatcgatcg cgaggccttc gcgcagcgtc cacaaatcgc gcgcctgccg ctcggaagtc gcgagtaccg catcgtcgac gagcgctgca tcgaaccccg cggcgagcgc cgtttcgagc gcatgatgcg catcgccgcc cggcgcgctc gtcgcgcatt cgatcaacac cacgaaaccg ccgtcgccga cgaacggtgc cgacacaccg ggcgtatgcc gcgcgacgta atcgtagaac gccggccaca tcgcctcgaa gctgacgact tccggcagcg tgcgcacgcg gttccacagc gcgaccaccg cgtcgtagtc gcgcacgcgg cagagcgccg tggccggcgc cgcgagcaac ggatgcagcc gcagcaccgc gcgcgtgacg acgccgagcg tcccttcgct gccgatgaac agctgcttca ggtcgtagcc cgcgttgttc ttcagcatcc ggttcatcga cgacacgacc gtgccgtccg cgagcaccgc ttcgagcccc aacacctgct cgcgcatcat tccgtagcgg atcgcacgcg tgccgcccgc gttggtcgcg agcatgccgc cgatctggca cgagccgcgc gcgccgagat cgacgccgaa cgtgaagccc gccgcttccg cggcgtcctg cacggtctgc agcggcgtgc ccgcgcgcac ggtgatcgtg ccggccgccg cgtcgagcgc ttcgatgccc gcgaagcgct ccatcgacag cacgacctcg ccgcccagcg cgaccgcccc gcgcgcgagc ccggtcagcc cgccttgcgg cacgaccggc tggccgagcc gcatgcacag cgcgagcgcg cgcgacacgt cgtcgacgct gcgcgggcgc accagcgcac gcggccgcac gccggccggc tcgttgtacg cggtgaagta acgtggatcg atatcggccg cgcgcgtgac gagcgcgtcg cccagttcgg cggcgagcac cgcatcgacg gcgtcgtccg gcaacggcct gccgtccggt gcgcggcccg cgacggtcgg gttcggggtc ggcatcgaac caggaccctg ggtctgagct tccagggtac cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPTPNPTVA GRAPDGRPLP DDAVDAVLAA ELGDALVTRA ADIDPRYFTA YNEPAGVRPR ALVRPRSVDD VSRALALCMR LGQPVVPQGG LTGLARGAVA LGGEVVLSME RFAGIEALDA AAGTITVRAG TPLQTVQDAA EAAGFTFGVD LGARGSCQIG GMLATNAGGT RAIRYGMMRE QVLGLEAVLA DGTVVSSMNR MLKNNAGYDL KQLFIGSEGT LGVVTRAVLR LHPLLAAPAT ALCRVRDYDA VVALWNRVRT LPEVVSFEAM WPAFYDYVAR HTPGVSAPFV GDGGFVVLIE CATSAPGGDA HHALETALAA GFDAALVDDA VLATSERQAR DLWTLREGLA IDALPHLVNF DVSLPTGELG AFAERCEAVL RTRWPALTCL FFGHVGDGNV HIGVSLAAMT DADADALDHC VYAVVRDMGG SVSAEHGIGV LKRPYLAHTR SDAEIGLMRR LKAALDPRGI LNPGKVL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgaccccg aacccgaccg tcgcgggccg cgcaccggac ggcaggccgt tgccggacga cgccgtcgat gcggtgctcg ccgccgaact gggcgacgcg ctcgtcacgc gcgcggccga tatcgatcca cgttacttca ccgcgtacaa cgagccggcc ggcgtgcggc cgcgtgcgct ggtgcgcccg cgcagcgtcg acgacgtgtc gcgcgcgctc gcgctgtgca tgcggctcgg ccagccggtc gtgccgcaag gcgggctgac cgggctcgcg cgcggggcgg tcgcgctggg cggcgaggtc gtgctgtcga tggagcgctt cgcgggcatc gaagcgctcg acgcggcggc cggcacgatc accgtgcgcg cgggcacgcc gctgcagacc gtgcaggacg ccgcggaagc ggcgggcttc acgttcggcg tcgatctcgg cgcgcgcggc tcgtgccaga tcggcggcat gctcgcgacc aacgcgggcg gcacgcgtgc gatccgctac ggaatgatgc gcgagcaggt gttggggctc gaagcggtgc tcgcggacgg cacggtcgtg tcgtcgatga accggatgct gaagaacaac gcgggctacg acctgaagca gctgttcatc ggcagcgaag ggacgctcgg cgtcgtcacg cgcgcggtgc tgcggctgca tccgttgctc gcggcgccgg ccacggcgct ctgccgcgtg cgcgactacg acgcggtggt cgcgctgtgg aaccgcgtgc gcacgctgcc ggaagtcgtc agcttcgagg cgatgtggcc ggcgttctac gattacgtcg cgcggcatac gcccggtgtg tcggcaccgt tcgtcggcga cggcggtttc gtggtgttga tcgaatgcgc gacgagcgcg ccgggcggcg atgcgcatca tgcgctcgaa acggcgctcg ccgcggggtt cgatgcagcg ctcgtcgacg atgcggtact cgcgacttcc gagcggcagg cgcgcgattt gtggacgctg cgcgaaggcc tcgcgatcga tgcgctgccc catctcgtca acttcgacgt gagtctgccg accggcgaac tcggcgcgtt cgccgagcgc tgcgaggcgg tgttgcggac gcgctggccg gcgctcacgt gcctgttctt cggccatgtc ggcgacggca acgtgcatat cggcgtgtcg ctggccgcga tgaccgacgc ggacgccgat gcgctcgatc actgcgtgta tgcggtggtg cgcgacatgg gcgggtcggt gtcggccgag cacgggatcg gcgtgctcaa gcgtccttat ctcgcgcaca cgcgcagcga cgcggaaatc gggctgatgc gacgcctgaa ggccgcgctg gacccgcgcg gcatcctcaa tccgggcaag gtcctttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BuceA.18020.a.A1.PS01276
PURIFICATION DATe: 11/8/2011
CONCENTRATION: 60.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MPTPNPTVAG RAPDGRPLPD DAVDAVLAAE LGDALVTRAA DIDPRYFTAY NEPAGVRPRA LVRPRSVDDV SRALALCMRL GQPVVPQGGL TGLARGAVAL GGEVVLSMER FAGIEALDAA AGTITVRAGT PLQTVQDAAE AAGFTFGVDL GARGSCQIGG MLATNAGGTR AIRYGMMREQ VLGLEAVLAD GTVVSSMNRM LKNNAGYDLK QLFIGSEGTL GVVTRAVLRL HPLLAAPATA LCRVRDYDAV VALWNRVRTL PEVVSFEAMW PAFYDYVARH TPGVSAPFVG DGGFVVLIEC ATSAPGGDAH HALETALAAG FDAALVDDAV LATSERQARD LWTLREGLAI DALPHLVNFD VSLPTGELGA FAERCEAVLR TRWPALTCLF FGHVGDGNVH IGVSLAAMTD ADADALDHCV YAVVRDMGGS VSAEHGIGVL KRPYLAHTRS DAEIGLMRRL K
Validated NT Sequence
gccttcaggc gtcgcatcag cccgatttcc gcgtcgctgc gcgtgtgcgc gagataagga cgcttgagca cgccgatccc gtgctcggcc gacaccgacc cgcccatgtc gcgcaccacc gcatacacgc agtgatcgag cgcatcggcg tccgcgtcgg tcatcgcggc cagcgacacg ccgatatgca cgttgccgtc gccgacatgg ccgaagaaca ggcacgtgag cgccggccag cgcgtccgca acaccgcctc gcagcgctcg gcgaacgcgc cgagttcgcc ggtcggcaga ctcacgtcga agttgacgag atggggcagc gcatcgatcg cgaggccttc gcgcagcgtc cacaaatcgc gcgcctgccg ctcggaagtc gcgagtaccg catcgtcgac gagcgctgca tcgaaccccg cggcgagcgc cgtttcgagc gcatgatgcg catcgccgcc cggcgcgctc gtcgcgcatt cgatcaacac cacgaaaccg ccgtcgccga cgaacggtgc cgacacaccg ggcgtatgcc gcgcgacgta atcgtagaac gccggccaca tcgcctcgaa gctgacgact tccggcagcg tgcgcacgcg gttccacagc gcgaccaccg cgtcgtagtc gcgcacgcgg cagagcgccg tggccggcgc cgcgagcaac ggatgcagcc gcagcaccgc gcgcgtgacg acgccgagcg tcccttcgct gccgatgaac agctgcttca ggtcgtagcc cgcgttgttc ttcagcatcc ggttcatcga cgacacgacc gtgccgtccg cgagcaccgc ttcgagcccc aacacctgct cgcgcatcat tccgtagcgg atcgcacgcg tgccgcccgc gttggtcgcg agcatgccgc cgatctggca cgagccgcgc gcgccgagat cgacgccgaa cgtgaagccc gccgcttccg cggcgtcctg cacggtctgc agcggcgtgc ccgcgcgcac ggtgatcgtg ccggccgccg cgtcgagcgc ttcgatgccc gcgaagcgct ccatcgacag cacgacctcg ccgcccagcg cgaccgcccc gcgcgcgagc ccggtcagcc cgccttgcgg cacgaccggc tggccgagcc gcatgcacag cgcgagcgcg cgcgacacgt cgtcgacgct gcgcgggcgc accagcgcac gcggccgcac gccggccggc tcgttgtacg cggtgaagta acgtggatcg atatcggccg cgcgcgtgac gagcgcgtcg cccagttcgg cggcgagcac cgcatcgacg gcgtcgtccg gcaacggcct gccgtccggt gcgcggcccg cgacggtcgg gttcggggtc ggcatcgaac caggaccctg ggtctgagct tccagggtac cc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPTPNPTVA GRAPDGRPLP DDAVDAVLAA ELGDALVTRA ADIDPRYFTA YNEPAGVRPR ALVRPRSVDD VSRALALCMR LGQPVVPQGG LTGLARGAVA LGGEVVLSME RFAGIEALDA AAGTITVRAG TPLQTVQDAA EAAGFTFGVD LGARGSCQIG GMLATNAGGT RAIRYGMMRE QVLGLEAVLA DGTVVSSMNR MLKNNAGYDL KQLFIGSEGT LGVVTRAVLR LHPLLAAPAT ALCRVRDYDA VVALWNRVRT LPEVVSFEAM WPAFYDYVAR HTPGVSAPFV GDGGFVVLIE CATSAPGGDA HHALETALAA GFDAALVDDA VLATSERQAR DLWTLREGLA IDALPHLVNF DVSLPTGELG AFAERCEAVL RTRWPALTCL FFGHVGDGNV HIGVSLAAMT DADADALDHC VYAVVRDMGG SVSAEHGIGV LKRPYLAHTR SDAEIGLMRR LKAALDPRGI LNPGKVL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGACCCCG AACCCGACCG TCGCGGGCCG CGCACCGGAC GGCAGGCCGT TGCCGGACGA CGCCGTCGAT GCGGTGCTCG CCGCCGAACT GGGCGACGCG CTCGTCACGC GCGCGGCCGA TATCGATCCA CGTTACTTCA CCGCGTACAA CGAGCCGGCC GGCGTGCGGC CGCGTGCGCT GGTGCGCCCG CGCAGCGTCG ACGACGTGTC GCGCGCGCTC GCGCTGTGCA TGCGGCTCGG CCAGCCGGTC GTGCCGCAAG GCGGGCTGAC CGGGCTCGCG CGCGGGGCGG TCGCGCTGGG CGGCGAGGTC GTGCTGTCGA TGGAGCGCTT CGCGGGCATC GAAGCGCTCG ACGCGGCGGC CGGCACGATC ACCGTGCGCG CGGGCACGCC GCTGCAGACC GTGCAGGACG CCGCGGAAGC GGCGGGCTTC ACGTTCGGCG TCGATCTCGG CGCGCGCGGC TCGTGCCAGA TCGGCGGCAT GCTCGCGACC AACGCGGGCG GCACGCGTGC GATCCGCTAC GGAATGATGC GCGAGCAGGT GTTGGGGCTC GAAGCGGTGC TCGCGGACGG CACGGTCGTG TCGTCGATGA ACCGGATGCT GAAGAACAAC GCGGGCTACG ACCTGAAGCA GCTGTTCATC GGCAGCGAAG GGACGCTCGG CGTCGTCACG CGCGCGGTGC TGCGGCTGCA TCCGTTGCTC GCGGCGCCGG CCACGGCGCT CTGCCGCGTG CGCGACTACG ACGCGGTGGT CGCGCTGTGG AACCGCGTGC GCACGCTGCC GGAAGTCGTC AGCTTCGAGG CGATGTGGCC GGCGTTCTAC GATTACGTCG CGCGGCATAC GCCCGGTGTG TCGGCACCGT TCGTCGGCGA CGGCGGTTTC GTGGTGTTGA TCGAATGCGC GACGAGCGCG CCGGGCGGCG ATGCGCATCA TGCGCTCGAA ACGGCGCTCG CCGCGGGGTT CGATGCAGCG CTCGTCGACG ATGCGGTACT CGCGACTTCC GAGCGGCAGG CGCGCGATTT GTGGACGCTG CGCGAAGGCC TCGCGATCGA TGCGCTGCCC CATCTCGTCA ACTTCGACGT GAGTCTGCCG ACCGGCGAAC TCGGCGCGTT CGCCGAGCGC TGCGAGGCGG TGTTGCGGAC GCGCTGGCCG GCGCTCACGT GCCTGTTCTT CGGCCATGTC GGCGACGGCA ACGTGCATAT CGGCGTGTCG CTGGCCGCGA TGACCGACGC GGACGCCGAT GCGCTCGATC ACTGCGTGTA TGCGGTGGTG CGCGACATGG GCGGGTCGGT GTCGGCCGAG CACGGGATCG GCGTGCTCAA GCGTCCTTAT CTCGCGCACA CGCGCAGCGA CGCGGAAATC GGGCTGATGC GACGCCTGAA GGCCGCGCTG GACCCGCGCG GCATCCTCAA TCCGGGCAAG GTCCTTTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA