BuceA.18019.a

Putative polygalacturonase

CENTER ID: BuceA.18019.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18019.a.A1.GE34661 full length 1 725
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6888
RefSeq: YP_002153588.1
UniProt: B4EPW3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTLSVARPHS RPDSPPSRRP RALGLLAALL ATAAALTACG GGDASTGGGA ASTAPPATSV FQVGTTTVDP NLPPEPALPA DTQVCSTLEA ANTLVSRPDG SLPPEADPST AGVGKAVSTA TANPDQARIQ AALDACGAAV DAEVGATIAA ADAAATAAQK TAAAPNANLA GASGEELAKP KYRASKFAVR LVVNRAGPGN GFISGPLTLP SGVTLWIDKG ATLYASRDVK AYSPNAAGPY CGNTAVSATK AGSSSNCAAL ITGTNLVNAA VVGDGRIDGR GYAELVTSDA KYPLMKVDLT CSNTYAAYKS GTVAPDGTAC DDGGTVVDSK STVRNMTWWD LAYLGNMVQN GTTGFGSQSN FRLMVFNYAK NLTLYRITLN NSPNFHVVPS GVDGLTIWNV KVQTPTLAAF ANPAGNGNPL YNGQTFNADN VKNTDAFDPG SASKPTSVAL STGSTTTSAA AISFDGYLKN FVFAYNYVST GDDDIALKGS NNPAPAGSGL FGIDGNRDVR ADRKWGVVIA HNHIYWGHGI SIGSETNGGV TNVQVYDNAF RDSEEGLRIK SDYARGGEVS NIHYANICIR DAKNALLFTP YYSTKAVPAS GPLYPNFHDI SLANVVIEGA AGVKLQGFEA NTGGYGEPAF PLVMSLTDVV ADTPDGIATI ASDANLTLRN VNLPVFPSTA NRVVVNGSAV HAIDPASVLD CSKAFVDFPG IDQSNPFGST WAPAL
NT Sequence
atgacacttt ccgttgcccg gccacattcc cggccggatt ccccgcccag ccgacgcccg cgtgcgctcg gcctgctcgc cgcgctgctc gccaccgccg ccgcgctcac cgcgtgcggc ggcggcgacg cgagtaccgg cggcggcgcg gcgtcgaccg caccgcccgc gaccagcgtg ttccaggtcg gcacgacgac cgtcgacccg aacctgccac ccgaaccggc gttgcccgcc gacacgcagg tgtgcagcac gctcgaagcc gccaatacgc tcgtgagccg ccccgacggc tcgctgccgc cggaggccga tccgtcgacg gccggcgtcg gcaaggccgt gtcgaccgcg acggccaacc ccgaccaggc gcgcatccag gccgcgctcg acgcctgcgg cgcggcggtc gacgccgaag tgggcgcgac gatcgcggcg gcggacgccg cggccaccgc cgcgcagaag acggccgccg cgccgaacgc gaacctcgcc ggcgcgtccg gcgaggaact cgcgaagccg aagtaccgcg cgagcaagtt cgcggtgcgg ctcgtcgtca atcgcgcggg gcccggcaac ggcttcatca gcgggccgct gacgctgccg tccggcgtca cgctgtggat cgacaagggc gccacgctgt atgcgtcgcg cgacgtgaag gcgtattcgc cgaacgccgc cggcccgtac tgcggcaaca cggccgtcag cgcgacgaaa gccggcagct cgtcgaactg cgccgcgctg atcaccggca ccaacctcgt caacgcggcc gtggtcggcg acggccggat tgacgggcgc ggctacgcgg agctcgtgac gtccgatgcg aaatacccgc tgatgaaggt cgacctgacc tgctcgaaca cctacgcggc gtacaagagc ggcaccgtgg cgcccgacgg caccgcatgc gacgacggcg gcacggtcgt cgattcgaaa tcgacggtgc gcaacatgac gtggtgggat ctcgcgtatc tcggcaacat ggtgcagaac ggcacgaccg gcttcggctc gcagtcgaat ttccggctga tggtgttcaa ctacgcgaag aacctcacgc tgtaccgcat cacgctgaac aacagcccga atttccacgt ggtgccgagc ggcgtcgacg ggctgacgat ctggaacgtg aaggtgcaga cgccgacgct cgccgcgttc gcgaacccgg ccggcaacgg caacccgctg tacaacgggc agacgttcaa cgcggacaac gtgaagaaca ccgacgcgtt cgacccgggc tccgcgtcga agccgacgtc ggtggcgctg tcgaccggca gcacgacgac gtccgccgcc gcgatctcgt tcgacggcta cctgaagaac ttcgtgttcg cgtacaacta cgtgagcacc ggcgacgacg acatcgcgct caagggcagc aacaatccgg cgcccgccgg ctcggggctg ttcggcatcg acggcaatcg cgacgtgcgc gccgatcgca aatggggcgt cgtgatcgcg cacaaccaca tctactgggg gcacggcatc tcgatcggca gcgagaccaa cggcggcgtg accaacgttc aggtgtacga caacgcgttc cgcgattcgg aagaaggact gcgcatcaag tccgactatg cgcgcggcgg cgaagtgagc aacatccact acgcgaatat ctgcatccgc gatgcgaaga acgcgctgct gttcacgccg tactacagca ccaaggccgt gcccgcgagc ggcccgctgt atccgaactt ccacgacatc tcgctcgcga acgtcgtgat cgagggcgcg gccggcgtga agctgcaggg cttcgaggcg aacaccggcg gctacggcga gccggcgttc ccgctcgtga tgtcgctgac cgacgtggtg gccgatacgc ccgacggcat cgcgacgatc gcgtccgacg cgaacctcac gctgcgcaac gtgaacctgc cggtgttccc gtcgaccgcg aaccgggtgg tcgtcaacgg cagcgcggtc catgcgatcg atccggccag cgtgctcgac tgcagcaagg cgttcgtcga cttcccgggc atcgaccagt cgaacccgtt cgggtcgacg tgggcgccgg cgctgtaa
Details for BuceA.18019.a.A1.GE34661
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/20/2012
EXPECTED MW: 76kDa
OBSERVED MW: 76kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 57
PERCENT COVERAGE: 22
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTLSVARPH SRPDSPPSRR PRALGLLAAL LATAXXXXXX VCFILPVHAS SPKPGDFANT QARHIATFFP GRMTGTPAEM LSADYIRQQF QQMGYRSDIR TFNSRYIYTA RDNRKSWHNV TGSTVIAAHE GKAPQXIIIM AHLDTYAP
Validated NT Sequence
aggagatata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatgac actttccgtt gcccggccac attcccggcc ggattccccg cccagccgac gcccgcgtgc gctcggcctg ctcgccgcgc tgctcgccac cgctnannnn nactnnnnnn ncgtatgctt tattctcccc gtacacgcct cgtcacctaa acctggcgat tttgctaata ctcaggcacg acatattgct actttctttc cgggacgcat gaccggaact cctgcagaaa tgttatctgc cgattatatt cgccaacagt ttcagcaaat gggttatcgc agtgatattc ggacatttaa tagtcggtat atttataccg cccgcgataa tcgtaagagc tggcataacg tgacgggaag tacggtgatt gccgctcatg aaggcaaagc gccgcagcan atcatcatta tggcgcatct ggatacttac gccccgct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTLSVARPH SRPDSPPSRR PRALGLLAAL LATAAALTAC GGGDASTGGG AASTAPPATS VFQVGTTTVD PNLPPEPALP ADTQVCSTLE AANTLVSRPD GSLPPEADPS TAGVGKAVST ATANPDQARI QAALDACGAA VDAEVGATIA AADAAATAAQ KTAAAPNANL AGASGEELAK PKYRASKFAV RLVVNRAGPG NGFISGPLTL PSGVTLWIDK GATLYASRDV KAYSPNAAGP YCGNTAVSAT KAGSSSNCAA LITGTNLVNA AVVGDGRIDG RGYAELVTSD AKYPLMKVDL TCSNTYAAYK SGTVAPDGTA CDDGGTVVDS KSTVRNMTWW DLAYLGNMVQ NGTTGFGSQS NFRLMVFNYA KNLTLYRITL NNSPNFHVVP SGVDGLTIWN VKVQTPTLAA FANPAGNGNP LYNGQTFNAD NVKNTDAFDP GSASKPTSVA LSTGSTTTSA AAISFDGYLK NFVFAYNYVS TGDDDIALKG SNNPAPAGSG LFGIDGNRDV RADRKWGVVI AHNHIYWGHG ISIGSETNGG VTNVQVYDNA FRDSEEGLRI KSDYARGGEV SNIHYANICI RDAKNALLFT PYYSTKAVPA SGPLYPNFHD ISLANVVIEG AAGVKLQGFE ANTGGYGEPA FPLVMSLTDV VADTPDGIAT IASDANLTLR NVNLPVFPST ANRVVVNGSA VHAIDPASVL DCSKAFVDFP GIDQSNPFGS TWAPAL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacactttcc gttgcccggc cacattcccg gccggattcc ccgcccagcc gacgcccgcg tgcgctcggc ctgctcgccg cgctgctcgc caccgccgcc gcgctcaccg cgtgcggcgg cggcgacgcg agtaccggcg gcggcgcggc gtcgaccgca ccgcccgcga ccagcgtgtt ccaggtcggc acgacgaccg tcgacccgaa cctgccaccc gaaccggcgt tgcccgccga cacgcaggtg tgcagcacgc tcgaagccgc caatacgctc gtgagccgcc ccgacggctc gctgccgccg gaggccgatc cgtcgacggc cggcgtcggc aaggccgtgt cgaccgcgac ggccaacccc gaccaggcgc gcatccaggc cgcgctcgac gcctgcggcg cggcggtcga cgccgaagtg ggcgcgacga tcgcggcggc ggacgccgcg gccaccgccg cgcagaagac ggccgccgcg ccgaacgcga acctcgccgg cgcgtccggc gaggaactcg cgaagccgaa gtaccgcgcg agcaagttcg cggtgcggct cgtcgtcaat cgcgcggggc ccggcaacgg cttcatcagc gggccgctga cgctgccgtc cggcgtcacg ctgtggatcg acaagggcgc cacgctgtat gcgtcgcgcg acgtgaaggc gtattcgccg aacgccgccg gcccgtactg cggcaacacg gccgtcagcg cgacgaaagc cggcagctcg tcgaactgcg ccgcgctgat caccggcacc aacctcgtca acgcggccgt ggtcggcgac ggccggattg acgggcgcgg ctacgcggag ctcgtgacgt ccgatgcgaa atacccgctg atgaaggtcg acctgacctg ctcgaacacc tacgcggcgt acaagagcgg caccgtggcg cccgacggca ccgcatgcga cgacggcggc acggtcgtcg attcgaaatc gacggtgcgc aacatgacgt ggtgggatct cgcgtatctc ggcaacatgg tgcagaacgg cacgaccggc ttcggctcgc agtcgaattt ccggctgatg gtgttcaact acgcgaagaa cctcacgctg taccgcatca cgctgaacaa cagcccgaat ttccacgtgg tgccgagcgg cgtcgacggg ctgacgatct ggaacgtgaa ggtgcagacg ccgacgctcg ccgcgttcgc gaacccggcc ggcaacggca acccgctgta caacgggcag acgttcaacg cggacaacgt gaagaacacc gacgcgttcg acccgggctc cgcgtcgaag ccgacgtcgg tggcgctgtc gaccggcagc acgacgacgt ccgccgccgc gatctcgttc gacggctacc tgaagaactt cgtgttcgcg tacaactacg tgagcaccgg cgacgacgac atcgcgctca agggcagcaa caatccggcg cccgccggct cggggctgtt cggcatcgac ggcaatcgcg acgtgcgcgc cgatcgcaaa tggggcgtcg tgatcgcgca caaccacatc tactgggggc acggcatctc gatcggcagc gagaccaacg gcggcgtgac caacgttcag gtgtacgaca acgcgttccg cgattcggaa gaaggactgc gcatcaagtc cgactatgcg cgcggcggcg aagtgagcaa catccactac gcgaatatct gcatccgcga tgcgaagaac gcgctgctgt tcacgccgta ctacagcacc aaggccgtgc ccgcgagcgg cccgctgtat ccgaacttcc acgacatctc gctcgcgaac gtcgtgatcg agggcgcggc cggcgtgaag ctgcagggct tcgaggcgaa caccggcggc tacggcgagc cggcgttccc gctcgtgatg tcgctgaccg acgtggtggc cgatacgccc gacggcatcg cgacgatcgc gtccgacgcg aacctcacgc tgcgcaacgt gaacctgccg gtgttcccgt cgaccgcgaa ccgggtggtc gtcaacggca gcgcggtcca tgcgatcgat ccggccagcg tgctcgactg cagcaaggcg ttcgtcgact tcccgggcat cgaccagtcg aacccgttcg ggtcgacgtg ggcgccggcg ctgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa