SSGCID: Target Page

BuceA.17969.a

Putative uncharacterized protein

CENTER ID:	BuceA.17969.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17969.a.B1.GE35022	full length		1	342

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17969.a.B1.PW35637	full length		1	342

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2934
RefSeq:	YP_002231937.1
UniProt:	B4EA19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSDLPKHVT PAQIDAAAAL LDAGQLVAFP TETVYGLGGD AANPEAVARI YAAKGRPANH PVIVHLPPGG DPGYWADDLP ADAHALIDAF WPGPLTLILK RHARIPDAVS GGQDSVGLRC PSHPVAQALL AAFSARRGGH GGVAAPSANR FGHVSPTTAQ HVRDEFGDTV HVLDGGASEV GIESTILDLS RGFPALLRPG HVTPQQIADV LGRAPKLPDG SDATAPRASG TLKAHYAPRT PLALLPFDAL EPLLAAAQTD GERIALVARA SRAGRWAQAD GVHFVAAPED PQAYARDLYG MLRALDRAQV ARILVEKLPE TVEWIAVNDR LGRAAAAFEA RD

NT Sequence

atgtccagcg atctcccgaa gcacgtgacg cccgcgcaga tcgacgcggc tgccgcgttg ctcgacgcgg gccagctcgt cgcgtttccg accgaaaccg tctacgggct cggcggcgat gcggcgaacc ccgaggccgt cgcacgcatc tacgcggcga aggggcggcc cgcgaaccat ccggtgatcg tgcatctgcc gccgggcggc gatcccggtt actgggccga cgacttgccg gccgacgcgc acgcgctgat cgacgcgttc tggccgggcc cgctgacgct gatcctgaag cgccacgcgc gcattccgga tgccgtgagc ggcgggcagg attcggtcgg gctgcgctgc ccgtcgcatc cggtcgcgca ggcgctgctg gccgcgttca gcgcgcggcg cggcggccac ggcggcgtgg ctgcgccgtc cgcgaaccgg ttcggccacg tgagtccgac gaccgcgcaa cacgtgcgcg acgaattcgg cgataccgtg cacgtgctcg acggcggcgc atccgaagtc ggcatcgagt cgacgattct cgacctgtcg cgtggtttcc cggcgctgct gcgcccgggg cacgtgacgc cgcaacagat cgccgacgtg ctcggccgcg ccccgaagct gccggacggc agcgatgcga cggccccacg cgcgtcgggc acgctgaaag cgcattacgc gccgcgcacg ccgctcgcgc tgctgccgtt cgacgcgctc gagccgctgc tcgcggccgc gcaaaccgac ggcgaacgca tcgcgctcgt cgcccgcgcg tcgcgtgcgg gacgctgggc gcaggctgac ggcgtgcact tcgtcgcggc gccggaagat ccgcaagcct atgcgcgcga cctgtacgga atgctgcgcg cgctcgaccg ggcgcaggtc gcacgcattc tcgtcgagaa gctgcccgag accgtcgaat ggatcgcggt caacgatcgc ctcggccgcg cggccgcggc gttcgaagcg cgcgactga

Details for BuceA.17969.a.B1.GE35022

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	22
PERCENT COVERAGE:	66

Validated AA Sequence

MRFQRARRAW GRRIAAVRQL RGAAEHVGDL LRRHVPRAQQ RRETTRQVEN RRLDADFGCA AVEHVHGIAE FVAHVLRGRR THVAEPVRGR RSHAAVAAAP RAERGQQRLR DRMRRAAQPD RILPAAHGIR NARVALQDQR QRARPERVDQ RVRVGRQVVG PVTGIAARRQ MHDHRMVRGP PLRRVDACDG LGVRRIAAEP VDGFGRKRDE LARVEQRGSR VDLRGRHVLR EI

Validated NT Sequence

tagtcgcgcg cttcgaacgc cgcggccgcg cggccgaggc gatcgttgac cgcgatccat tcgacggtct cgggcagctt ctcgacgaga atgcgtgcga cctgcgcccg gtcgagcgcg cgcagcattc cgtacaggtc gcgcgcatag gcttgcggat cttccggcgc cgcgacgaag tgcacgccgt cagcctgcgc ccagcgtccc gcacgcgacg cgcgggcgac gagcgcgatg cgttcgccgt cggtttgcgc ggccgcgagc agcggctcga gcgcgtcgaa cggcagcagc gcgagcggcg tgcgcggcgc gtaatgcgct ttcagcgtgc ccgacgcgcg tggggccgtc gcatcgctgc cgtccggcag cttcggggcg cggccgagca cgtcggcgat ctgttgcggc gtcacgtgcc ccgggcgcag cagcgccggg aaaccacgcg acaggtcgag aatcgtcgac tcgatgccga cttcggatgc gccgccgtcg agcacgtgca cggtatcgcc gaattcgtcg cgcacgtgtt gcgcggtcgt cggactcacg tggccgaacc ggttcgcgga cggcgcagcc acgccgccgt ggccgccgcg ccgcgcgctg aacgcggcca gcagcgcctg cgcgaccgga tgcgacgggc agcgcagccc gaccgaatcc tgcccgccgc tcacggcatc cggaatgcgc gcgtggcgct tcaggatcag cgtcagcggg cccggccaga acgcgtcgat cagcgcgtgc gcgtcggccg gcaagtcgtc ggcccagtaa ccgggatcgc cgcccggcgg cagatgcacg atcaccggat ggttcgcggg ccgccccttc gccgcgtaga tgcgtgcgac ggcctcgggg ttcgccgcat cgccgccgag cccgtagacg gtttcggtcg gaaacgcgac gagctggccc gcgtcgagca acgcggcagc cgcgtcgatc tgcgcgggcg tcacgtgctt cgggagatcg c

Expected Protein Sequence

MAHHHHHHMS SDLPKHVTPA QIDAAAALLD AGQLVAFPTE TVYGLGGDAA NPEAVARIYA AKGRPANHPV IVHLPPGGDP GYWADDLPAD AHALIDAFWP GPLTLILKRH ARIPDAVSGG QDSVGLRCPS HPVAQALLAA FSARRGGHGG VAAPSANRFG HVSPTTAQHV RDEFGDTVHV LDGGASEVGI ESTILDLSRG FPALLRPGHV TPQQIADVLG RAPKLPDGSD ATAPRASGTL KAHYAPRTPL ALLPFDALEP LLAAAQTDGE RIALVARASR AGRWAQADGV HFVAAPEDPQ AYARDLYGML RALDRAQVAR ILVEKLPETV EWIAVNDRLG RAAAAFEARD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca gcgatctccc gaagcacgtg acgcccgcgc agatcgacgc ggctgccgcg ttgctcgacg cgggccagct cgtcgcgttt ccgaccgaaa ccgtctacgg gctcggcggc gatgcggcga accccgaggc cgtcgcacgc atctacgcgg cgaaggggcg gcccgcgaac catccggtga tcgtgcatct gccgccgggc ggcgatcccg gttactgggc cgacgacttg ccggccgacg cgcacgcgct gatcgacgcg ttctggccgg gcccgctgac gctgatcctg aagcgccacg cgcgcattcc ggatgccgtg agcggcgggc aggattcggt cgggctgcgc tgcccgtcgc atccggtcgc gcaggcgctg ctggccgcgt tcagcgcgcg gcgcggcggc cacggcggcg tggctgcgcc gtccgcgaac cggttcggcc acgtgagtcc gacgaccgcg caacacgtgc gcgacgaatt cggcgatacc gtgcacgtgc tcgacggcgg cgcatccgaa gtcggcatcg agtcgacgat tctcgacctg tcgcgtggtt tcccggcgct gctgcgcccg gggcacgtga cgccgcaaca gatcgccgac gtgctcggcc gcgccccgaa gctgccggac ggcagcgatg cgacggcccc acgcgcgtcg ggcacgctga aagcgcatta cgcgccgcgc acgccgctcg cgctgctgcc gttcgacgcg ctcgagccgc tgctcgcggc cgcgcaaacc gacggcgaac gcatcgcgct cgtcgcccgc gcgtcgcgtg cgggacgctg ggcgcaggct gacggcgtgc acttcgtcgc ggcgccggaa gatccgcaag cctatgcgcg cgacctgtac ggaatgctgc gcgcgctcga ccgggcgcag gtcgcacgca ttctcgtcga gaagctgccc gagaccgtcg aatggatcgc ggtcaacgat cgcctcggcc gcgcggccgc ggcgttcgaa gcgcgcgact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BuceA.17969.a.B1.PW35637

PURIFICATION DATe:	4/20/2012
CONCENTRATION:	35.31mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	200µl
PERCENT IDENTITY:	22
PERCENT COVERAGE:	66

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCA GCGATCTCCC GAAGCACGTG ACGCCCGCGC AGATCGACGC GGCTGCCGCG TTGCTCGACG CGGGCCAGCT CGTCGCGTTT CCGACCGAAA CCGTCTACGG GCTCGGCGGC GATGCGGCGA ACCCCGAGGC CGTCGCACGC ATCTACGCGG CGAAGGGGCG GCCCGCGAAC CATCCGGTGA TCGTGCATCT GCCGCCGGGC GGCGATCCCG GTTACTGGGC CGACGACTTG CCGGCCGACG CGCACGCGCT GATCGACGCG TTCTGGCCGG GCCCGCTGAC GCTGATCCTG AAGCGCCACG CGCGCATTCC GGATGCCGTG AGCGGCGGGC AGGATTCGGT CGGGCTGCGC TGCCCGTCGC ATCCGGTCGC GCAGGCGCTG CTGGCCGCGT TCAGCGCGCG GCGCGGCGGC CACGGCGGCG TGGCTGCGCC GTCCGCGAAC CGGTTCGGCC ACGTGAGTCC GACGACCGCG CAACACGTGC GCGACGAATT CGGCGATACC GTGCACGTGC TCGACGGCGG CGCATCCGAA GTCGGCATCG AGTCGACGAT TCTCGACCTG TCGCGTGGTT TCCCGGCGCT GCTGCGCCCG GGGCACGTGA CGCCGCAACA GATCGCCGAC GTGCTCGGCC GCGCCCCGAA GCTGCCGGAC GGCAGCGATG CGACGGCCCC ACGCGCGTCG GGCACGCTGA AAGCGCATTA CGCGCCGCGC ACGCCGCTCG CGCTGCTGCC GTTCGACGCG CTCGAGCCGC TGCTCGCGGC CGCGCAAACC GACGGCGAAC GCATCGCGCT CGTCGCCCGC GCGTCGCGTG CGGGACGCTG GGCGCAGGCT GACGGCGTGC ACTTCGTCGC GGCGCCGGAA GATCCGCAAG CCTATGCGCG CGACCTGTAC GGAATGCTGC GCGCGCTCGA CCGGGCGCAG GTCGCACGCA TTCTCGTCGA GAAGCTGCCC GAGACCGTCG AATGGATCGC GGTCAACGAT CGCCTCGGCC GCGCGGCCGC GGCGTTCGAA GCGCGCGACT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT