SSGCID: Target Page

BuceA.17935.a

60 kDa chaperonin 3 (GroEL protein 3) (Protein Cpn60 3)

CENTER ID:	BuceA.17935.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17935.a.B1.GE37060	full length		1	540

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17935.a.B1.PS01898	full length		1	540

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.7347
RefSeq:	YP_002154019.1
UniProt:	B4EP31

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTAKDVKFRD GARQQIVKGV NVLADAVKVT LGPKGRNVVI ERGFGAPVIT KDGVSVAKEI ELKDRFENMG AQIVKQVASK TADVAGDGTT TATVLAQAIV QEGMKHVAAG MNPMDLKRGI DKAVGAVLDE LRKLSRPIST HKEIAQVGAI SANSDDAIGK IIADAMEKVG KDGVITVEDG KSMENELDVV EGMQFDRGYV SPYFINDPAK QAAYLDDALI LLHDKKISSV RDLLPILEAA SKAGKPLLIV AEDVEAEALA TLVVNSMRGI LKVAAVKAPG FGDRRKAMLE DLAILTGATV ISEETGKQLD KATLEDLGSA KRVEVRKDDT IIIDGAGDAA RIDARVKAIR AQIDEATSDY DREKLQERVA KLAGGVAVIK VGAVTEVEMK EKKDRVDDAL HATRAAVEEG IVPGGGVALL RARAALSDIR GANADQDAGI RIVLRALEAP LRVIAANAGD EPSVVISKVL EGKGNFGYNA ATGEYGDLVD AGVVDPTKVT RTALQNAASI ASLVLTTDAT VAQAPKEDGA EPAAAPDLGY

NT Sequence

atgactgcaa aagacgtgaa attccgcgac ggcgcgcgcc agcagatcgt caagggcgtg aacgtgctgg ccgatgcggt gaaggtgacg ctcggtccga agggccgcaa cgtcgtgatc gagcgcggct tcggcgcgcc cgtgatcacg aaggacggcg tgtcggtcgc gaaggaaatc gagctgaagg accgcttcga gaacatgggc gcgcagatcg tcaagcaggt cgcgtcgaag acggccgacg tggccggcga cgggaccacc accgccaccg tgctcgcgca ggcgatcgtg caggaaggca tgaagcacgt cgcggccggc atgaacccga tggatctgaa gcgcggcatc gacaaggccg tcggcgcggt actcgacgag ttgcgcaagc tgtcccgacc gatttcgacc cacaaggaaa tcgcgcaggt cggcgcgatt tccgcgaact cggacgacgc gatcggcaag atcatcgccg atgcgatgga gaaggtcggc aaggacggcg tgatcaccgt cgaggacggc aagtcgatgg agaacgaact cgacgtggtc gagggcatgc agttcgatcg cggttacgtg agcccgtatt tcatcaacga tcccgccaag caggctgcct atctcgacga tgcgctgatt ctgctgcatg acaagaagat ctccagtgtc cgcgatctgc tgccgatcct cgaagcggcg tccaaggccg gcaagccgct gctgatcgtc gccgaggacg tcgaggccga agcgctcgcg acactcgtcg tgaattcgat gcgcggcatc ctgaaggtcg ccgccgtcaa ggcgccgggc ttcggcgacc ggcgcaaggc gatgctggaa gatctcgcga tcctgaccgg cgcgacggtc atctcggagg aaaccggcaa gcagctcgac aaggccacgc tggaggatct tggcagtgcg aagcgcgtcg aggtgcgcaa ggacgatacg atcatcatcg acggcgcagg cgatgcggcg cgcatcgacg cgcgcgtgaa ggcgattcgc gcgcagatcg acgaagcgac gagcgactac gatcgcgaga agctgcagga gcgtgtcgcg aagctcgcgg gcggcgtggc ggtgatcaag gtcggcgccg tcaccgaagt ggaaatgaag gagaagaagg accgcgtcga cgatgcgctg catgcgacgc gtgccgcggt cgaggagggc atcgtgccgg gcggtggcgt cgcgctgctg cgtgcgcgcg cggcgctgtc cgacatccgg ggcgcgaatg cggaccagga tgccggcatc cggatcgtgc tgcgtgcgct ggaagcgccg ctgcgcgtca tcgcggccaa cgcgggcgac gaaccgtcgg tcgtgatctc gaaggtgctc gaaggcaaag ggaacttcgg ctacaacgcg gcgaccggtg agtacggcga tctggtcgat gcgggcgtgg tcgatccgac caaggtcacg cggaccgcgc tgcagaacgc ggcatcgatc gcgagcctgg tgctgacgac cgatgcgacc gtcgcgcaag cgccgaaaga ggacggcgcc gagccggccg ccgcgcccga cctcggctac tga

Details for BuceA.17935.a.B1.GE37060

HARVESTED ON:	6/12/2013
SEQUENCED ON:	6/11/2013
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	18
PERCENT COVERAGE:	45

Validated AA Sequence

MTRSGASSAR STIRMPASWS AFXPRMSDSA ARARXXATPP PGTMPSSTAA RVACSASSTR SFFSFXSXSV TAPTLITATP PAXXXTRSCX FXRSXSLVXS SICARIXXXR ASMRAAXXAX SMMIVSSXXX XXRFXXXRSS XVXLSSCLXX SSEMTVAPVR IARSSSIXLR RSPKPGXXRR RPSGCRAXXS RRVSRALRXR RXXRRSAAAC RPWTPLRGSA ADRGHWRSSC HAAESAHRRD RQPAWRDR

Validated NT Sequence

gctcggcgcc gtcctctttc ggcgcttgcg cgacggtcgc atcggtcgtc agcaccaggc tcgcgatcga tgccgcgttc tgcagcgcgg tccgcgtgac cttggtcgga tcgaccacgc ccgcatcgac cagatcgccg tactcaccgg tcgccgcgtt gtagccgaag ttccctttgc cttcgagcac cttcgagatc acgaccgacg gttcgtcgcc cgcgttggcc gcgatgacgc gcagcggcgc ttccagcgca cgcagcacga tccggatgcc ggcatcctgg tccgcattcn cgccccggat gtcggacagc gccgcgcgcg cacgcancan cgcgacgcca ccgcccggca cgatgccctc ctcgaccgcg gcacgcgtcg catgcagcgc atcgtcgacg cggtccttct tctccttcnt ttccncttcn gtgacggcgc cgaccttgat caccgccacg ccgcccgcga gntncnngac acgctcntgc nncttcnngc gatcgnantc gctcgtcnct tcntcgatct gcgcgcgaat cgncttnnnn cgngcgtcna tgcgcgccgc ancgnctgcn ncgtcgatga tgatcgtatc gtcnnngnnn nnnnnnnnnc gcttcnnant gnnnagatcc tcnagngtgn ncttgtcgag ctgcttgnnn nnttcctccg agatgaccgt cgcgccggtc aggatcgcga gatcttccag catcnccttg cgccgntcgc cgaagcccgg cnnnngacgg cggcgacctt caggatgccg cgcatngant tcacgacgag tgtcgcgagc gcttcggcnt cgacgtcntn ggcgacgatc agcagcggct tgccgncctt ggacgccgct tcgaggatcg gcagcagatc gcggacactg gagatcttct tgtcatgcag cagaatcagc gcatcgtcga gataggcagc ctgcttggcg ggatcgttga tgaaatacgg gctcacgtaa ccgcgatcga actgcatgcc ctcgaccacg tcgagttcgt tctccatcga cttgccgtcc tcgacggtga tcacgccgtc cttgccgacc ttctccatcg catcggcgat gatcttgccg atcgcgtcgt ccgagttcgc ggaaatcgcg ccgacctgcg cgatttcctt gtgggtcgaa atcggtcggg acagcttgcg caactcgtcg agtaccgcgc cgacggcctt gtcgatgccg cgcttcagat ccatcgggtt catgccggcc gcgacgtgct tcatgccttc ctgcacgatc gcctgcgcga gcacggtggc ggtggtggtc ccgtcgccgg ccacgtcggc cgtcttcgac gcgacctgct tgacgatctg cgcgcccatg ttctcgaagc ggtccttcag ctcgatttcc ttcgcgaccg acacgccgtc cttcgtgatc acgggcgcgc cgaagccgcg ctcgatcacg acgttgcggc ccttcggacc gagcgtcacc ttcaccgcat cggccagcac gttcacgccc ttgacgatct gctggcgcgc gccgtcgcgg aatttcacgt cttttg

Expected Protein Sequence

MAHHHHHHMT AKDVKFRDGA RQQIVKGVNV LADAVKVTLG PKGRNVVIER GFGAPVITKD GVSVAKEIEL KDRFENMGAQ IVKQVASKTA DVAGDGTTTA TVLAQAIVQE GMKHVAAGMN PMDLKRGIDK AVGAVLDELR KLSRPISTHK EIAQVGAISA NSDDAIGKII ADAMEKVGKD GVITVEDGKS MENELDVVEG MQFDRGYVSP YFINDPAKQA AYLDDALILL HDKKISSVRD LLPILEAASK AGKPLLIVAE DVEAEALATL VVNSMRGILK VAAVKAPGFG DRRKAMLEDL AILTGATVIS EETGKQLDKA TLEDLGSAKR VEVRKDDTII IDGAGDAARI DARVKAIRAQ IDEATSDYDR EKLQERVAKL AGGVAVIKVG AVTEVEMKEK KDRVDDALHA TRAAVEEGIV PGGGVALLRA RAALSDIRGA NADQDAGIRI VLRALEAPLR VIAANAGDEP SVVISKVLEG KGNFGYNAAT GEYGDLVDAG VVDPTKVTRT ALQNAASIAS LVLTTDATVA QAPKEDGAEP AAAPDLGY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg caaaagacgt gaaattccgc gacggcgcgc gccagcagat cgtcaagggc gtgaacgtgc tggccgatgc ggtgaaggtg acgctcggtc cgaagggccg caacgtcgtg atcgagcgcg gcttcggcgc gcccgtgatc acgaaggacg gcgtgtcggt cgcgaaggaa atcgagctga aggaccgctt cgagaacatg ggcgcgcaga tcgtcaagca ggtcgcgtcg aagacggccg acgtggccgg cgacgggacc accaccgcca ccgtgctcgc gcaggcgatc gtgcaggaag gcatgaagca cgtcgcggcc ggcatgaacc cgatggatct gaagcgcggc atcgacaagg ccgtcggcgc ggtactcgac gagttgcgca agctgtcccg accgatttcg acccacaagg aaatcgcgca ggtcggcgcg atttccgcga actcggacga cgcgatcggc aagatcatcg ccgatgcgat ggagaaggtc ggcaaggacg gcgtgatcac cgtcgaggac ggcaagtcga tggagaacga actcgacgtg gtcgagggca tgcagttcga tcgcggttac gtgagcccgt atttcatcaa cgatcccgcc aagcaggctg cctatctcga cgatgcgctg attctgctgc atgacaagaa gatctccagt gtccgcgatc tgctgccgat cctcgaagcg gcgtccaagg ccggcaagcc gctgctgatc gtcgccgagg acgtcgaggc cgaagcgctc gcgacactcg tcgtgaattc gatgcgcggc atcctgaagg tcgccgccgt caaggcgccg ggcttcggcg accggcgcaa ggcgatgctg gaagatctcg cgatcctgac cggcgcgacg gtcatctcgg aggaaaccgg caagcagctc gacaaggcca cgctggagga tcttggcagt gcgaagcgcg tcgaggtgcg caaggacgat acgatcatca tcgacggcgc aggcgatgcg gcgcgcatcg acgcgcgcgt gaaggcgatt cgcgcgcaga tcgacgaagc gacgagcgac tacgatcgcg agaagctgca ggagcgtgtc gcgaagctcg cgggcggcgt ggcggtgatc aaggtcggcg ccgtcaccga agtggaaatg aaggagaaga aggaccgcgt cgacgatgcg ctgcatgcga cgcgtgccgc ggtcgaggag ggcatcgtgc cgggcggtgg cgtcgcgctg ctgcgtgcgc gcgcggcgct gtccgacatc cggggcgcga atgcggacca ggatgccggc atccggatcg tgctgcgtgc gctggaagcg ccgctgcgcg tcatcgcggc caacgcgggc gacgaaccgt cggtcgtgat ctcgaaggtg ctcgaaggca aagggaactt cggctacaac gcggcgaccg gtgagtacgg cgatctggtc gatgcgggcg tggtcgatcc gaccaaggtc acgcggaccg cgctgcagaa cgcggcatcg atcgcgagcc tggtgctgac gaccgatgcg accgtcgcgc aagcgccgaa agaggacggc gccgagccgg ccgccgcgcc cgacctcggc tactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for BuceA.17935.a.B1.PS01898

PURIFICATION DATe:	9/12/2013
CONCENTRATION:	73.5mg/ml
OBSERVED MW:	66kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	18
PERCENT COVERAGE:	45

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTG CAAAAGACGT GAAATTCCGC GACGGCGCGC GCCAGCAGAT CGTCAAGGGC GTGAACGTGC TGGCCGATGC GGTGAAGGTG ACGCTCGGTC CGAAGGGCCG CAACGTCGTG ATCGAGCGCG GCTTCGGCGC GCCCGTGATC ACGAAGGACG GCGTGTCGGT CGCGAAGGAA ATCGAGCTGA AGGACCGCTT CGAGAACATG GGCGCGCAGA TCGTCAAGCA GGTCGCGTCG AAGACGGCCG ACGTGGCCGG CGACGGGACC ACCACCGCCA CCGTGCTCGC GCAGGCGATC GTGCAGGAAG GCATGAAGCA CGTCGCGGCC GGCATGAACC CGATGGATCT GAAGCGCGGC ATCGACAAGG CCGTCGGCGC GGTACTCGAC GAGTTGCGCA AGCTGTCCCG ACCGATTTCG ACCCACAAGG AAATCGCGCA GGTCGGCGCG ATTTCCGCGA ACTCGGACGA CGCGATCGGC AAGATCATCG CCGATGCGAT GGAGAAGGTC GGCAAGGACG GCGTGATCAC CGTCGAGGAC GGCAAGTCGA TGGAGAACGA ACTCGACGTG GTCGAGGGCA TGCAGTTCGA TCGCGGTTAC GTGAGCCCGT ATTTCATCAA CGATCCCGCC AAGCAGGCTG CCTATCTCGA CGATGCGCTG ATTCTGCTGC ATGACAAGAA GATCTCCAGT GTCCGCGATC TGCTGCCGAT CCTCGAAGCG GCGTCCAAGG CCGGCAAGCC GCTGCTGATC GTCGCCGAGG ACGTCGAGGC CGAAGCGCTC GCGACACTCG TCGTGAATTC GATGCGCGGC ATCCTGAAGG TCGCCGCCGT CAAGGCGCCG GGCTTCGGCG ACCGGCGCAA GGCGATGCTG GAAGATCTCG CGATCCTGAC CGGCGCGACG GTCATCTCGG AGGAAACCGG CAAGCAGCTC GACAAGGCCA CGCTGGAGGA TCTTGGCAGT GCGAAGCGCG TCGAGGTGCG CAAGGACGAT ACGATCATCA TCGACGGCGC AGGCGATGCG GCGCGCATCG ACGCGCGCGT GAAGGCGATT CGCGCGCAGA TCGACGAAGC GACGAGCGAC TACGATCGCG AGAAGCTGCA GGAGCGTGTC GCGAAGCTCG CGGGCGGCGT GGCGGTGATC AAGGTCGGCG CCGTCACCGA AGTGGAAATG AAGGAGAAGA AGGACCGCGT CGACGATGCG CTGCATGCGA CGCGTGCCGC GGTCGAGGAG GGCATCGTGC CGGGCGGTGG CGTCGCGCTG CTGCGTGCGC GCGCGGCGCT GTCCGACATC CGGGGCGCGA ATGCGGACCA GGATGCCGGC ATCCGGATCG TGCTGCGTGC GCTGGAAGCG CCGCTGCGCG TCATCGCGGC CAACGCGGGC GACGAACCGT CGGTCGTGAT CTCGAAGGTG CTCGAAGGCA AAGGGAACTT CGGCTACAAC GCGGCGACCG GTGAGTACGG CGATCTGGTC GATGCGGGCG TGGTCGATCC GACCAAGGTC ACGCGGACCG CGCTGCAGAA CGCGGCATCG ATCGCGAGCC TGGTGCTGAC GACCGATGCG ACCGTCGCGC AAGCGCCGAA AGAGGACGGC GCCGAGCCGG CCGCCGCGCC CGACCTCGGC TACTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT