BuceA.17895.a

Multifunctional CCA protein [Includes: CCA-adding enzyme (EC 2.7.7.72) (CCA tRNA nucleotidyltransferase) (tRNA CCA-pyrophosphorylase) (tRNA adenylyl-/cytidylyl-transferase) (tRNA nucleotidyltransferase) (tRNA-NT); 2-prime-nucleotidase (EC 3.1.3.-); 2-prime-,3-prime-cyclic phosphodiesterase (EC 3.1.4.-); Phosphatase (EC 3.1.3.-)]

CENTER ID: BuceA.17895.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.17895.a.B1.GE35033 full length 1 413
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.17895.a.B1.PW36256 full length 1 413

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.581
RefSeq: YP_002229719.1
UniProt: B4E8K1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIYAVGGAI RDELLGVPVQ DRDYVVVGAT PEQMTAQGFR PVGKDFPVFL HPQTQEEYAL ARTERKTAAG YHGFQFHYAP DVTLDEDLAR RDLTINAMAR EVSPEGTLVG PVIDPFDGQA DLRARVFRHV SDAFVEDPVR ILRIARFAAR FADFTVADET LALMRRMVDA GEVDALVPER VWQEIARGLM EAKPSRMFAV LRDCGALARI LPEVDALWGV PQRADYHPEV DTGVHVMMVV DYAAKQGYSL AVRFAALTHD LGKGTTPADV LPRHVGHESR SVELLKPLCE RLRVPNECRD LALVVAREHG NLHRVMEMGA AALVRLFERS DALRKPARFA ELLQACESDA RGRLGLDAQP YPQAERLRVA LAAARSVDAG AIARGIGSDT EKIKEAVHRA RIQAVAQALA IGE
NT Sequence
atgaacatct acgcagtagg cggcgcgatc cgcgacgaat tgctcggcgt gccggtgcag gaccgcgact acgtggtggt cggcgcgacg cccgagcaga tgaccgcgca gggcttccgg ccggtcggca aggatttccc ggtgttcctg catccgcaga cgcaagagga gtacgcgctg gcgcgcaccg agcgcaagac cgcagccggc tatcacggct tccagttcca ctacgcgccg gacgtcacgc tcgacgagga tctcgcgcgg cgcgacctga cgatcaacgc gatggcgcgc gaggtgagcc cggaaggcac gctggtcggc ccggtgatcg atccgttcga cgggcaggcc gacctgcgcg cgcgtgtgtt ccggcacgtg agcgacgcgt tcgtcgagga tccggtgcgg atcctgcgga tcgcgcgctt tgccgcgcgg ttcgcggatt tcacggtcgc ggacgaaacg ctcgcgctga tgcggcggat ggtcgacgcg ggcgaggtcg acgcgctcgt ccccgaacgc gtgtggcagg aaatcgcgcg cgggctgatg gaggcgaagc cgtcgcggat gttcgcggtg ctgcgcgact gcggcgcgct cgcgcgcatc ctgcccgagg tcgatgcgct gtggggcgtg ccgcagcgcg ccgattacca cccggaagtc gataccggcg tgcacgtgat gatggtcgtc gactacgcgg cgaagcaggg ctattcgctg gcggtgcgct tcgcggcgct cacgcacgat ctcggcaagg ggacgacgcc cgcggacgtg ctgccgcgcc acgtcggcca cgaaagccgc agcgtcgagc tgctcaagcc gttgtgcgag cggctgcggg tgccgaacga atgccgcgat ctcgcgctgg tggtcgcgcg cgagcacggc aacctgcatc gcgtgatgga aatgggcgcg gccgcgctgg tgcggctgtt cgagcgcagc gacgcgctgc gcaagccggc gcgcttcgcc gagctgctgc aggcctgcga atcggatgcg cgcggccggc tcggactcga tgcgcagccg tatccgcagg ccgagcggtt gcgcgtcgcg ctcgcggccg cgcgcagcgt cgatgccggc gcgatcgcac gcggcatcgg cagcgacacg gagaagatca aggaagccgt gcatcgcgcg cggatccagg ccgtcgcgca ggcgctcgca atcggcgagt ag
Details for BuceA.17895.a.B1.GE35033
HARVESTED ON: 3/19/2012
SEQUENCED ON: 3/22/2012
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMN IYAVGGAIRD ELLGVPVQDR DYVVVGATPE QMTAQGFRPV GKDFPVFLHP QTQEEYALAR TERKTAAGYH GFQFHYAPDV TLDEDLARRD LTINAMAREV SPEGTLVGPV IDPFDGQADL RARVFRHVSD AFVEDPVRIL RIARFAARFA DFTVADETLA LMRRMVDAGE VDALVPERVW QEIARGLMEA KPSRMFAVLR DCGALARILP EVDALWGVPQ RADYHPEVDT GVHVMMVVDY AAKQGYSLAV RFAALTHDLG KGTTPADVLP RHVGHESRSV ELLKPLCERL RVPNECRDLA LVVAREHGNL HRVMEMGAAA LVRLFERSDA LRKPARFAEL LQACESDARG RLGLDAQPYP QAERLRVALA AARSVDAGAI ARGIGSDTEK IKEAVHRARI
Validated NT Sequence
ataccatggc tcaccaccac caccaccata tgaacatcta cgcagtaggc ggcgcgatcc gcgacgaatt gctcggcgtg ccggtgcagg accgcgacta cgtggtggtc ggcgcgacgc ccgagcagat gaccgcgcag ggcttccggc cggtcggcaa ggatttcccg gtgttcctgc atccgcagac gcaagaggag tacgcgctgg cgcgcaccga gcgcaagacc gcagccggct atcacggctt ccagttccac tacgcgccgg acgtcacgct cgacgaggat ctcgcgcggc gcgacctgac gatcaacgcg atggcgcgcg aggtgagccc ggaaggcacg ctggtcggcc cggtgatcga tccgttcgac gggcaggccg acctgcgcgc gcgtgtgttc cggcacgtga gcgacgcgtt cgtcgaggat ccggtgcgga tcctgcggat cgcgcgcttt gccgcgcggt tcgcggattt cacggtcgcg gacgaaacgc tcgcgctgat gcggcggatg gtcgacgcgg gcgaggtcga cgcgctcgtc cccgaacgcg tgtggcagga aatcgcgcgc gggctgatgg aggcgaagcc gtcgcggatg ttcgcggtgc tgcgcgactg cggcgcgctc gcgcgcatcc tgcccgaggt cgatgcgctg tggggcgtgc cgcagcgcgc cgattaccac ccggaagtcg ataccggcgt gcacgtgatg atggtcgtcg actacgcggc gaagcagggc tattcgctgg cggtgcgctt cgcggcgctc acgcacgatc tcggcaaggg gacgacgccc gcggacgtgc tgccgcgcca cgtcggccac gaaagccgca gcgtcgagct gctcaagccg ttgtgcgagc ggctgcgggt gccgaacgaa tgccgcgatc tcgcgctggt ggtcgcgcgc gagcacggca acctgcatcg cgtgatggaa atgggcgcgg ccgcgctggt gcggctgttc gagcgcagcg acgcgctgcg caagccggcg cgcttcgccg agctgctgca ggcctgcgaa tcggatgcgc gcggccggct cggactcgat gcgcagccgt atccgcaggc cgagcggttg cgcgtcgcgc tcgcggccgc gcgcagcgtc gatgccggcg cgatcgcacg cggcatcggc agcgacacgg agaagatcaa ggaagccgtg catcgcgcgc ggatccag
Expected Protein Sequence
MAHHHHHHMN IYAVGGAIRD ELLGVPVQDR DYVVVGATPE QMTAQGFRPV GKDFPVFLHP QTQEEYALAR TERKTAAGYH GFQFHYAPDV TLDEDLARRD LTINAMAREV SPEGTLVGPV IDPFDGQADL RARVFRHVSD AFVEDPVRIL RIARFAARFA DFTVADETLA LMRRMVDAGE VDALVPERVW QEIARGLMEA KPSRMFAVLR DCGALARILP EVDALWGVPQ RADYHPEVDT GVHVMMVVDY AAKQGYSLAV RFAALTHDLG KGTTPADVLP RHVGHESRSV ELLKPLCERL RVPNECRDLA LVVAREHGNL HRVMEMGAAA LVRLFERSDA LRKPARFAEL LQACESDARG RLGLDAQPYP QAERLRVALA AARSVDAGAI ARGIGSDTEK IKEAVHRARI QAVAQALAIG E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca tctacgcagt aggcggcgcg atccgcgacg aattgctcgg cgtgccggtg caggaccgcg actacgtggt ggtcggcgcg acgcccgagc agatgaccgc gcagggcttc cggccggtcg gcaaggattt cccggtgttc ctgcatccgc agacgcaaga ggagtacgcg ctggcgcgca ccgagcgcaa gaccgcagcc ggctatcacg gcttccagtt ccactacgcg ccggacgtca cgctcgacga ggatctcgcg cggcgcgacc tgacgatcaa cgcgatggcg cgcgaggtga gcccggaagg cacgctggtc ggcccggtga tcgatccgtt cgacgggcag gccgacctgc gcgcgcgtgt gttccggcac gtgagcgacg cgttcgtcga ggatccggtg cggatcctgc ggatcgcgcg ctttgccgcg cggttcgcgg atttcacggt cgcggacgaa acgctcgcgc tgatgcggcg gatggtcgac gcgggcgagg tcgacgcgct cgtccccgaa cgcgtgtggc aggaaatcgc gcgcgggctg atggaggcga agccgtcgcg gatgttcgcg gtgctgcgcg actgcggcgc gctcgcgcgc atcctgcccg aggtcgatgc gctgtggggc gtgccgcagc gcgccgatta ccacccggaa gtcgataccg gcgtgcacgt gatgatggtc gtcgactacg cggcgaagca gggctattcg ctggcggtgc gcttcgcggc gctcacgcac gatctcggca aggggacgac gcccgcggac gtgctgccgc gccacgtcgg ccacgaaagc cgcagcgtcg agctgctcaa gccgttgtgc gagcggctgc gggtgccgaa cgaatgccgc gatctcgcgc tggtggtcgc gcgcgagcac ggcaacctgc atcgcgtgat ggaaatgggc gcggccgcgc tggtgcggct gttcgagcgc agcgacgcgc tgcgcaagcc ggcgcgcttc gccgagctgc tgcaggcctg cgaatcggat gcgcgcggcc ggctcggact cgatgcgcag ccgtatccgc aggccgagcg gttgcgcgtc gcgctcgcgg ccgcgcgcag cgtcgatgcc ggcgcgatcg cacgcggcat cggcagcgac acggagaaga tcaaggaagc cgtgcatcgc gcgcggatcc aggccgtcgc gcaggcgctc gcaatcggcg agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuceA.17895.a.B1.PW36256
PURIFICATION DATe: 5/11/2012
CONCENTRATION: 47.7mg/ml
OBSERVED MW: 39kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN IYAVGGAIRD ELLGVPVQDR DYVVVGATPE QMTAQGFRPV GKDFPVFLHP QTQEEYALAR TERKTAAGYH GFQFHYAPDV TLDEDLARRD LTINAMAREV SPEGTLVGPV IDPFDGQADL RARVFRHVSD AFVEDPVRIL RIARFAARFA DFTVADETLA LMRRMVDAGE VDALVPERVW QEIARGLMEA KPSRMFAVLR DCGALARILP EVDALWGVPQ RADYHPEVDT GVHVMMVVDY AAKQGYSLAV RFAALTHDLG KGTTPADVLP RHVGHESRSV ELLKPLCERL RVPNECRDLA LVVAREHGNL HRVMEMGAAA LVRLFERSDA LRKPARFAEL LQACESDARG RLGLDAQPYP QAERLRVALA AARSVDAGAI ARGIGSDTEK IKEAVHRARI
Validated NT Sequence
ataccatggc tcaccaccac caccaccata tgaacatcta cgcagtaggc ggcgcgatcc gcgacgaatt gctcggcgtg ccggtgcagg accgcgacta cgtggtggtc ggcgcgacgc ccgagcagat gaccgcgcag ggcttccggc cggtcggcaa ggatttcccg gtgttcctgc atccgcagac gcaagaggag tacgcgctgg cgcgcaccga gcgcaagacc gcagccggct atcacggctt ccagttccac tacgcgccgg acgtcacgct cgacgaggat ctcgcgcggc gcgacctgac gatcaacgcg atggcgcgcg aggtgagccc ggaaggcacg ctggtcggcc cggtgatcga tccgttcgac gggcaggccg acctgcgcgc gcgtgtgttc cggcacgtga gcgacgcgtt cgtcgaggat ccggtgcgga tcctgcggat cgcgcgcttt gccgcgcggt tcgcggattt cacggtcgcg gacgaaacgc tcgcgctgat gcggcggatg gtcgacgcgg gcgaggtcga cgcgctcgtc cccgaacgcg tgtggcagga aatcgcgcgc gggctgatgg aggcgaagcc gtcgcggatg ttcgcggtgc tgcgcgactg cggcgcgctc gcgcgcatcc tgcccgaggt cgatgcgctg tggggcgtgc cgcagcgcgc cgattaccac ccggaagtcg ataccggcgt gcacgtgatg atggtcgtcg actacgcggc gaagcagggc tattcgctgg cggtgcgctt cgcggcgctc acgcacgatc tcggcaaggg gacgacgccc gcggacgtgc tgccgcgcca cgtcggccac gaaagccgca gcgtcgagct gctcaagccg ttgtgcgagc ggctgcgggt gccgaacgaa tgccgcgatc tcgcgctggt ggtcgcgcgc gagcacggca acctgcatcg cgtgatggaa atgggcgcgg ccgcgctggt gcggctgttc gagcgcagcg acgcgctgcg caagccggcg cgcttcgccg agctgctgca ggcctgcgaa tcggatgcgc gcggccggct cggactcgat gcgcagccgt atccgcaggc cgagcggttg cgcgtcgcgc tcgcggccgc gcgcagcgtc gatgccggcg cgatcgcacg cggcatcggc agcgacacgg agaagatcaa ggaagccgtg catcgcgcgc ggatccag
Expressed Protein Sequence
MAHHHHHHMN IYAVGGAIRD ELLGVPVQDR DYVVVGATPE QMTAQGFRPV GKDFPVFLHP QTQEEYALAR TERKTAAGYH GFQFHYAPDV TLDEDLARRD LTINAMAREV SPEGTLVGPV IDPFDGQADL RARVFRHVSD AFVEDPVRIL RIARFAARFA DFTVADETLA LMRRMVDAGE VDALVPERVW QEIARGLMEA KPSRMFAVLR DCGALARILP EVDALWGVPQ RADYHPEVDT GVHVMMVVDY AAKQGYSLAV RFAALTHDLG KGTTPADVLP RHVGHESRSV ELLKPLCERL RVPNECRDLA LVVAREHGNL HRVMEMGAAA LVRLFERSDA LRKPARFAEL LQACESDARG RLGLDAQPYP QAERLRVALA AARSVDAGAI ARGIGSDTEK IKEAVHRARI QAVAQALAIG E
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACA TCTACGCAGT AGGCGGCGCG ATCCGCGACG AATTGCTCGG CGTGCCGGTG CAGGACCGCG ACTACGTGGT GGTCGGCGCG ACGCCCGAGC AGATGACCGC GCAGGGCTTC CGGCCGGTCG GCAAGGATTT CCCGGTGTTC CTGCATCCGC AGACGCAAGA GGAGTACGCG CTGGCGCGCA CCGAGCGCAA GACCGCAGCC GGCTATCACG GCTTCCAGTT CCACTACGCG CCGGACGTCA CGCTCGACGA GGATCTCGCG CGGCGCGACC TGACGATCAA CGCGATGGCG CGCGAGGTGA GCCCGGAAGG CACGCTGGTC GGCCCGGTGA TCGATCCGTT CGACGGGCAG GCCGACCTGC GCGCGCGTGT GTTCCGGCAC GTGAGCGACG CGTTCGTCGA GGATCCGGTG CGGATCCTGC GGATCGCGCG CTTTGCCGCG CGGTTCGCGG ATTTCACGGT CGCGGACGAA ACGCTCGCGC TGATGCGGCG GATGGTCGAC GCGGGCGAGG TCGACGCGCT CGTCCCCGAA CGCGTGTGGC AGGAAATCGC GCGCGGGCTG ATGGAGGCGA AGCCGTCGCG GATGTTCGCG GTGCTGCGCG ACTGCGGCGC GCTCGCGCGC ATCCTGCCCG AGGTCGATGC GCTGTGGGGC GTGCCGCAGC GCGCCGATTA CCACCCGGAA GTCGATACCG GCGTGCACGT GATGATGGTC GTCGACTACG CGGCGAAGCA GGGCTATTCG CTGGCGGTGC GCTTCGCGGC GCTCACGCAC GATCTCGGCA AGGGGACGAC GCCCGCGGAC GTGCTGCCGC GCCACGTCGG CCACGAAAGC CGCAGCGTCG AGCTGCTCAA GCCGTTGTGC GAGCGGCTGC GGGTGCCGAA CGAATGCCGC GATCTCGCGC TGGTGGTCGC GCGCGAGCAC GGCAACCTGC ATCGCGTGAT GGAAATGGGC GCGGCCGCGC TGGTGCGGCT GTTCGAGCGC AGCGACGCGC TGCGCAAGCC GGCGCGCTTC GCCGAGCTGC TGCAGGCCTG CGAATCGGAT GCGCGCGGCC GGCTCGGACT CGATGCGCAG CCGTATCCGC AGGCCGAGCG GTTGCGCGTC GCGCTCGCGG CCGCGCGCAG CGTCGATGCC GGCGCGATCG CACGCGGCAT CGGCAGCGAC ACGGAGAAGA TCAAGGAAGC CGTGCATCGC GCGCGGATCC AGGCCGTCGC GCAGGCGCTC GCAATCGGCG AGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT