BuceA.17888.a
Putative stringent starvation protein A
| CENTER ID: | BuceA.17888.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.17888.a.A1.GE33217 | full length | 1 | 203 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.17888.a.A1.PW33827 | full length | 1 | 203 |
Structures
4QQ7
DEPOSITED: 6/26/2014
DETERMINATION: XRay
CLONE: BuceA.17888.a.A1.GE33217
PROTEIN: BuceA.17888.a.A1.PW33827
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126817 |
| PATRIC ID: | fig|216591.5.peg.348 |
| RefSeq: | YP_002229497.1 |
| UniProt: | B4E6Q3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMVLYSGTTC PFSQRCRLVL FEKGMDFEIR DVDLFNKPED ISVMNPYGQV PILVERDLIL YESNIINEYI DERFPHPQLM PADPVQRARA RLFLLNFEKE LFVHVSTLEN EKGKAAEKNH EKARLAIRDR LTQLAPIFVK NKYMLGEEFS MLDVAIAPLL WRLDHYGIEL SKNAAPLMKY AERIFSRPAY IEALTPSEKV MRR
NT Sequence
atgatggttc tgtattccgg cacaacttgc ccgttctccc agcgttgccg gctggtgctg ttcgagaagg gcatggactt cgaaatccgc gacgtcgacc tgttcaacaa gccggaagac atttcggtga tgaacccgta cggtcaggtg ccgatcctgg tcgagcgcga cctgatcctg tacgaatcga acatcatcaa cgagtacatc gacgagcgct tcccgcatcc gcaactgatg ccggccgacc cggtgcagcg cgcccgtgcg cgcctgttcc tgctcaactt cgagaaggaa ctgttcgtcc acgtcagcac gctcgagaac gagaagggca aggcggcgga gaagaatcac gagaaggccc gcctcgcgat ccgcgatcgc ctgacgcaac tcgcgccgat cttcgtgaag aacaagtaca tgctcggcga agagttctcg atgctcgacg tcgcgatcgc accgctgctg tggcgcctgg atcactacgg catcgagctg tcgaagaacg ccgcgccgct gatgaagtac gccgaacgga tcttcagccg tccggcctac atcgaagcac tgacgccgtc cgaaaaggtc atgcgtcgtt aa
Details for BuceA.17888.a.A1.GE33217
| HARVESTED ON: | 8/30/2011 |
| SEQUENCED ON: | 9/2/2011 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMMVLYSGTT CPFSQRCRLV LFEKGMDFEI RDVDLFNKPE DISVMNPYGQ VPILVERDLI LYESNIINEY IDERFPHPQL MPADPVQRAR ARLFLLNFEK ELFVHVSTLE NEKGKAAEKN HEKARLAIRD RLTQLAPIFV KNKYMLGEEF SMLDVAIAPL LWRLDHYGIE LSKNAAPLMK YAERIFSRPA YIEALTPSEK VMRR
Validated NT Sequence
ttattaacga cgcatgacct tttcggacgg cgtcagtgct tcgatgtagg ccggacggct gaagatccgt tcggcgtact tcatcagcgg cgcggcgttc ttcgacagct cgatgccgta gtgatccagg cgccacagca gcggtgcgat cgcgacgtcg agcatcgaga actcttcgcc gagcatgtac ttgttcttca cgaagatcgg cgcgagttgc gtcaggcgat cgcggatcgc gaggcgggcc ttctcgtgat tcttctccgc cgccttgccc ttctcgttct cgagcgtgct gacgtggacg aacagttcct tctcgaagtt gagcaggaac aggcgcgcac gggcgcgctg caccgggtcg gccggcatca gttgcggatg cgggaagcgc tcgtcgatgt actcgttgat gatgttcgat tcgtacagga tcaggtcgcg ctcgaccagg atcggcacct gaccgtacgg gttcatcacc gaaatgtctt ccggcttgtt gaacaggtcg acgtcgcgga tttcgaagtc catgcccttc tcgaacagca ccagccggca acgctgggag aacgggcaag ttgtgccgga atacagaacc atcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMVLYSGTT CPFSQRCRLV LFEKGMDFEI RDVDLFNKPE DISVMNPYGQ VPILVERDLI LYESNIINEY IDERFPHPQL MPADPVQRAR ARLFLLNFEK ELFVHVSTLE NEKGKAAEKN HEKARLAIRD RLTQLAPIFV KNKYMLGEEF SMLDVAIAPL LWRLDHYGIE LSKNAAPLMK YAERIFSRPA YIEALTPSEK VMRR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatggttctg tattccggca caacttgccc gttctcccag cgttgccggc tggtgctgtt cgagaagggc atggacttcg aaatccgcga cgtcgacctg ttcaacaagc cggaagacat ttcggtgatg aacccgtacg gtcaggtgcc gatcctggtc gagcgcgacc tgatcctgta cgaatcgaac atcatcaacg agtacatcga cgagcgcttc ccgcatccgc aactgatgcc ggccgacccg gtgcagcgcg cccgtgcgcg cctgttcctg ctcaacttcg agaaggaact gttcgtccac gtcagcacgc tcgagaacga gaagggcaag gcggcggaga agaatcacga gaaggcccgc ctcgcgatcc gcgatcgcct gacgcaactc gcgccgatct tcgtgaagaa caagtacatg ctcggcgaag agttctcgat gctcgacgtc gcgatcgcac cgctgctgtg gcgcctggat cactacggca tcgagctgtc gaagaacgcc gcgccgctga tgaagtacgc cgaacggatc ttcagccgtc cggcctacat cgaagcactg acgccgtccg aaaaggtcat gcgtcgttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BuceA.17888.a.A1.PW33827
| PURIFICATION DATe: | 10/14/2011 |
| CONCENTRATION: | 30.46mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMMVLYSGTT CPFSQRCRLV LFEKGMDFEI RDVDLFNKPE DISVMNPYGQ VPILVERDLI LYESNIINEY IDERFPHPQL MPADPVQRAR ARLFLLNFEK ELFVHVSTLE NEKGKAAEKN HEKARLAIRD RLTQLAPIFV KNKYMLGEEF SMLDVAIAPL LWRLDHYGIE LSKNAAPLMK YAERIFSRPA YIEALTPSEK VMRR
Validated NT Sequence
ttattaacga cgcatgacct tttcggacgg cgtcagtgct tcgatgtagg ccggacggct gaagatccgt tcggcgtact tcatcagcgg cgcggcgttc ttcgacagct cgatgccgta gtgatccagg cgccacagca gcggtgcgat cgcgacgtcg agcatcgaga actcttcgcc gagcatgtac ttgttcttca cgaagatcgg cgcgagttgc gtcaggcgat cgcggatcgc gaggcgggcc ttctcgtgat tcttctccgc cgccttgccc ttctcgttct cgagcgtgct gacgtggacg aacagttcct tctcgaagtt gagcaggaac aggcgcgcac gggcgcgctg caccgggtcg gccggcatca gttgcggatg cgggaagcgc tcgtcgatgt actcgttgat gatgttcgat tcgtacagga tcaggtcgcg ctcgaccagg atcggcacct gaccgtacgg gttcatcacc gaaatgtctt ccggcttgtt gaacaggtcg acgtcgcgga tttcgaagtc catgcccttc tcgaacagca ccagccggca acgctgggag aacgggcaag ttgtgccgga atacagaacc atcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMVLYSGTT CPFSQRCRLV LFEKGMDFEI RDVDLFNKPE DISVMNPYGQ VPILVERDLI LYESNIINEY IDERFPHPQL MPADPVQRAR ARLFLLNFEK ELFVHVSTLE NEKGKAAEKN HEKARLAIRD RLTQLAPIFV KNKYMLGEEF SMLDVAIAPL LWRLDHYGIE LSKNAAPLMK YAERIFSRPA YIEALTPSEK VMRR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATGGTTCTG TATTCCGGCA CAACTTGCCC GTTCTCCCAG CGTTGCCGGC TGGTGCTGTT CGAGAAGGGC ATGGACTTCG AAATCCGCGA CGTCGACCTG TTCAACAAGC CGGAAGACAT TTCGGTGATG AACCCGTACG GTCAGGTGCC GATCCTGGTC GAGCGCGACC TGATCCTGTA CGAATCGAAC ATCATCAACG AGTACATCGA CGAGCGCTTC CCGCATCCGC AACTGATGCC GGCCGACCCG GTGCAGCGCG CCCGTGCGCG CCTGTTCCTG CTCAACTTCG AGAAGGAACT GTTCGTCCAC GTCAGCACGC TCGAGAACGA GAAGGGCAAG GCGGCGGAGA AGAATCACGA GAAGGCCCGC CTCGCGATCC GCGATCGCCT GACGCAACTC GCGCCGATCT TCGTGAAGAA CAAGTACATG CTCGGCGAAG AGTTCTCGAT GCTCGACGTC GCGATCGCAC CGCTGCTGTG GCGCCTGGAT CACTACGGCA TCGAGCTGTC GAAGAACGCC GCGCCGCTGA TGAAGTACGC CGAACGGATC TTCAGCCGTC CGGCCTACAT CGAAGCACTG ACGCCGTCCG AAAAGGTCAT GCGTCGTTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA