SSGCID: Target Page

BuceA.17737.a

Flagellin (Type II)

CENTER ID:	BuceA.17737.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17737.a.B1.GE35030	full length		1	384

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17737.a.B1.PS01522	full length		1	384

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.127
RefSeq:	YP_002229280.1
UniProt:	B4EDI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLGINSNINS LVAQQNLNGS QNALSQAITR LSSGKRINSA ADDAAGLAIS TRMQTQINGL NQGVSNANDG VSMIQTASSA LSSLTNSLQR IRQLAVQAST GTMSTTDQAA LQQEVSQQIQ EVNRIASQTT YNGTNILDGS AGIVSFQVGA NVGQTISLDL SQSMSAAKIG GGLVQKGQTV GTVTGLSLDN NGAYTGSGAT ITAINVLSDG KGGYTFTDQN GGAISQTVAQ SVFGANATTG TGTAVGNLTL QSGATGAGTS AAQQTAITNA IAQINAVNKP ATVSNLDIST VSGANVAMVS IDNALQTVNN VQAALGAAQN RFTAIATSQQ AESTDLSSAQ SQITDANFAQ ETANMSKHQV LQQAGISVLA QANSLPQQVL KLLQ

NT Sequence

atgctcggaa ttaacagcaa catcaactcg ctggttgcac agcagaacct caacggctcg caaaacgccc tgtcccaggc catcacgcgc ctgtcgtcgg gcaagcgcat caacagcgca gcggacgacg cagcaggcct cgcgatctcg acccggatgc agacccagat caacggtctg aaccagggcg tgtcgaacgc caacgacggc gtgtcgatga ttcaaacggc atcgagcgca ctgtcgtcgc tgacgaacag cctgcagcgt atccgccagc tggccgtcca ggcgtcgacg ggcacgatgt ccacgaccga ccaggccgca ctgcagcagg aagtttcgca gcagatccag gaagtgaacc gtatcgcgtc gcaaacgacg tacaacggca cgaacattct cgacggctcg gcaggcatcg tgtcgttcca ggtcggcgcg aacgtcggcc agacgatttc gctggacctg agccaaagca tgtcggccgc gaagatcggc ggcggcctgg tgcagaaggg ccagacggtc ggtacggtga cgggtctgtc gctcgacaac aacggcgcgt acaccggttc gggcgcaacg atcaccgcga tcaacgtgct gtccgacggt aagggtggct acacgttcac cgaccagaac ggcggcgcca tctcgcaaac cgttgcccag tccgtgttcg gcgcaaacgc aaccaccggc accggcacgg cagtcggcaa cctgacgctg cagtcgggcg caaccggcgc aggtacgtcc gcggcacaac agaccgcaat caccaatgca atcgctcaga tcaacgccgt caacaagccg gctaccgtgt cgaacctcga catcagcacg gtcagcgggg cgaacgtcgc gatggtgtcg atcgacaacg cgctgcagac ggtgaacaac gtgcaggcgg cactcggcgc ggcgcaaaac cgcttcacgg caatcgccac gtcgcagcag gcagaatcga ccgacctgtc gtcggcacag tcgcagatca ccgacgcgaa cttcgcgcag gaaaccgcga acatgtcgaa gcaccaggtg ctgcagcaag caggcatctc ggtgctcgcg caggcgaact cgctgccgca gcaggtcctg aagctcctgc agtaa

Details for BuceA.17737.a.B1.GE35030

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	82

Validated AA Sequence

MQTQINGLNQ GVSNANDGVS MIQTASSALS SLTNSLQRIR QLAVQASTGT MSTTDQAALQ QEVSQQIQEV NRIASQTTYN GTNILDGSAG IVSFQVGANV GQTISLDLSQ SMSAAKIGGG LVQKGQTVGT VTGLSLDNNG AYTGSGATIT AINVLSDGKG GYTFTDQNGG AISQTVAQSV FGANATTGTG TAVGNLTLQS GATGAGTSAA QQTAITNAIA QINAVNKPAT VSNLDISTVS GANVAMVSID NALQTVNNVQ AALGAAQNRF TAIATSQQAE STDLSSAQSQ ITDANFAQET ANMSKHQVLQ QAGISVLAQA NS

Validated NT Sequence

cagcgagttc gcctgcgcga gcaccgagat gcctgcttgc tgcagcacct ggtgcttcga catgttcgcg gtttcctgcg cgaagttcgc gtcggtgatc tgcgactgtg ccgacgacag gtcggtcgat tctgcctgct gcgacgtggc gattgccgtg aagcggtttt gcgccgcgcc gagtgccgcc tgcacgttgt tcaccgtctg cagcgcgttg tcgatcgaca ccatcgcgac gttcgccccg ctgaccgtgc tgatgtcgag gttcgacacg gtagccggct tgttgacggc gttgatctga gcgattgcat tggtgattgc ggtctgttgt gccgcggacg tacctgcgcc ggttgcgccc gactgcagcg tcaggttgcc gactgccgtg ccggtgccgg tggttgcgtt tgcgccgaac acggactggg caacggtttg cgagatggcg ccgccgttct ggtcggtgaa cgtgtagcca cccttaccgt cggacagcac gttgatcgcg gtgatcgttg cgcccgaacc ggtgtacgcg ccgttgttgt cgagcgacag acccgtcacc gtaccgaccg tctggccctt ctgcaccagg ccgccgccga tcttcgcggc cgacatgctt tggctcaggt ccagcgaaat cgtctggccg acgttcgcgc cgacctggaa cgacacgatg cctgccgagc cgtcgagaat gttcgtgccg ttgtacgtcg tttgcgacgc gatacggttc acttcctgga tctgctgcga aacttcctgc tgcagtgcgg cctggtcggt cgtggacatc gtgcccgtcg acgcctggac ggccagctgg cggatacgct gcaggctgtt cgtcagcgac gacagtgcgc tcgatgccgt ttgaatcatc gacacgccgt cgttggcgtt cgacacgccc tggttcagac cgttgatctg ggtctgcatc cgggtcgaga tcgcgaggcc tgctgcgtcg tccgctgcgc tgttgatgcg cttgcccgac gacaggcgcg tgatggcctg ggacagggcg ttttgcgagc cgttgaggtt ctgctgtgca accagcgagt tgatgttgct gttaattc

Expected Protein Sequence

MAHHHHHHML GINSNINSLV AQQNLNGSQN ALSQAITRLS SGKRINSAAD DAAGLAISTR MQTQINGLNQ GVSNANDGVS MIQTASSALS SLTNSLQRIR QLAVQASTGT MSTTDQAALQ QEVSQQIQEV NRIASQTTYN GTNILDGSAG IVSFQVGANV GQTISLDLSQ SMSAAKIGGG LVQKGQTVGT VTGLSLDNNG AYTGSGATIT AINVLSDGKG GYTFTDQNGG AISQTVAQSV FGANATTGTG TAVGNLTLQS GATGAGTSAA QQTAITNAIA QINAVNKPAT VSNLDISTVS GANVAMVSID NALQTVNNVQ AALGAAQNRF TAIATSQQAE STDLSSAQSQ ITDANFAQET ANMSKHQVLQ QAGISVLAQA NSLPQQVLKL LQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctcg gaattaacag caacatcaac tcgctggttg cacagcagaa cctcaacggc tcgcaaaacg ccctgtccca ggccatcacg cgcctgtcgt cgggcaagcg catcaacagc gcagcggacg acgcagcagg cctcgcgatc tcgacccgga tgcagaccca gatcaacggt ctgaaccagg gcgtgtcgaa cgccaacgac ggcgtgtcga tgattcaaac ggcatcgagc gcactgtcgt cgctgacgaa cagcctgcag cgtatccgcc agctggccgt ccaggcgtcg acgggcacga tgtccacgac cgaccaggcc gcactgcagc aggaagtttc gcagcagatc caggaagtga accgtatcgc gtcgcaaacg acgtacaacg gcacgaacat tctcgacggc tcggcaggca tcgtgtcgtt ccaggtcggc gcgaacgtcg gccagacgat ttcgctggac ctgagccaaa gcatgtcggc cgcgaagatc ggcggcggcc tggtgcagaa gggccagacg gtcggtacgg tgacgggtct gtcgctcgac aacaacggcg cgtacaccgg ttcgggcgca acgatcaccg cgatcaacgt gctgtccgac ggtaagggtg gctacacgtt caccgaccag aacggcggcg ccatctcgca aaccgttgcc cagtccgtgt tcggcgcaaa cgcaaccacc ggcaccggca cggcagtcgg caacctgacg ctgcagtcgg gcgcaaccgg cgcaggtacg tccgcggcac aacagaccgc aatcaccaat gcaatcgctc agatcaacgc cgtcaacaag ccggctaccg tgtcgaacct cgacatcagc acggtcagcg gggcgaacgt cgcgatggtg tcgatcgaca acgcgctgca gacggtgaac aacgtgcagg cggcactcgg cgcggcgcaa aaccgcttca cggcaatcgc cacgtcgcag caggcagaat cgaccgacct gtcgtcggca cagtcgcaga tcaccgacgc gaacttcgcg caggaaaccg cgaacatgtc gaagcaccag gtgctgcagc aagcaggcat ctcggtgctc gcgcaggcga actcgctgcc gcagcaggtc ctgaagctcc tgcagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BuceA.17737.a.B1.PS01522

PURIFICATION DATe:	6/11/2012
CONCENTRATION:	28.6mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	82

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTCG GAATTAACAG CAACATCAAC TCGCTGGTTG CACAGCAGAA CCTCAACGGC TCGCAAAACG CCCTGTCCCA GGCCATCACG CGCCTGTCGT CGGGCAAGCG CATCAACAGC GCAGCGGACG ACGCAGCAGG CCTCGCGATC TCGACCCGGA TGCAGACCCA GATCAACGGT CTGAACCAGG GCGTGTCGAA CGCCAACGAC GGCGTGTCGA TGATTCAAAC GGCATCGAGC GCACTGTCGT CGCTGACGAA CAGCCTGCAG CGTATCCGCC AGCTGGCCGT CCAGGCGTCG ACGGGCACGA TGTCCACGAC CGACCAGGCC GCACTGCAGC AGGAAGTTTC GCAGCAGATC CAGGAAGTGA ACCGTATCGC GTCGCAAACG ACGTACAACG GCACGAACAT TCTCGACGGC TCGGCAGGCA TCGTGTCGTT CCAGGTCGGC GCGAACGTCG GCCAGACGAT TTCGCTGGAC CTGAGCCAAA GCATGTCGGC CGCGAAGATC GGCGGCGGCC TGGTGCAGAA GGGCCAGACG GTCGGTACGG TGACGGGTCT GTCGCTCGAC AACAACGGCG CGTACACCGG TTCGGGCGCA ACGATCACCG CGATCAACGT GCTGTCCGAC GGTAAGGGTG GCTACACGTT CACCGACCAG AACGGCGGCG CCATCTCGCA AACCGTTGCC CAGTCCGTGT TCGGCGCAAA CGCAACCACC GGCACCGGCA CGGCAGTCGG CAACCTGACG CTGCAGTCGG GCGCAACCGG CGCAGGTACG TCCGCGGCAC AACAGACCGC AATCACCAAT GCAATCGCTC AGATCAACGC CGTCAACAAG CCGGCTACCG TGTCGAACCT CGACATCAGC ACGGTCAGCG GGGCGAACGT CGCGATGGTG TCGATCGACA ACGCGCTGCA GACGGTGAAC AACGTGCAGG CGGCACTCGG CGCGGCGCAA AACCGCTTCA CGGCAATCGC CACGTCGCAG CAGGCAGAAT CGACCGACCT GTCGTCGGCA CAGTCGCAGA TCACCGACGC GAACTTCGCG CAGGAAACCG CGAACATGTC GAAGCACCAG GTGCTGCAGC AAGCAGGCAT CTCGGTGCTC GCGCAGGCGA ACTCGCTGCC GCAGCAGGTC CTGAAGCTCC TGCAGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT