SSGCID: Target Page

BuceA.17622.a

Uncharacterized protein

CENTER ID:	BuceA.17622.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17622.a.B1.GE41152	full length		1	254

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.5660
UniProt:	B4EMD3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDQSQVFRAM MPGLTSASGA RVTVAYPSFP RPLPLVRLDR RGLVFRAAGA APLVCGLPRP ATLLVADEPL CSLRLVIRDI AATGDGRHDL TMQPSGAAGD ELLWHALRDR EPYRRIRHPL APVDLPVPID GEPHAAAGET AARPSRSAAF RLPCHSDALF FADWLEYHFD ELRALSQQHG PQLQLNQLER QVDDDEVGVR FVYDIDGHAT RHALTDCARE ACAWIETEMR SKYALPVAQE RFGAFAAHAR AGGM

NT Sequence

gtggaccagt ctcaggtctt tcgcgcgatg atgcccgggt tgacgagcgc cagcggcgcc cgcgtcacgg tcgcctatcc gtcgtttccc aggccgttgc cgctcgtgcg gctcgaccgc cgcgggctcg tgtttcgtgc cgccggcgcc gcaccgctcg tgtgcgggct gccgcgcccg gcaaccctgc tggtcgcgga cgagccgctg tgctcgctgc gcctcgtgat ccgcgacatc gccgcgaccg gcgacggccg ccacgacctg acgatgcagc cgtccggcgc cgccggtgac gaattgctgt ggcacgcgtt gcgcgatcgc gagccgtacc ggcgaattcg gcacccgctc gcgccggtcg atctgcccgt tcccatcgac ggcgaaccgc atgcggcggc cggcgaaacc gctgcgcgcc cgtcgcgcag cgcggctttc cgcctgccct gccacagcga cgcgctgttc ttcgcggact ggctcgaata tcacttcgat gaactgcgcg cattgtcgca acagcacggg ccgcaactgc agctcaatca gctcgaacga caggttgacg atgacgaggt cggcgtgcgt ttcgtctacg acatcgacgg gcatgcgacg cgacacgcat tgaccgactg cgcgcgcgag gcatgtgcat ggatcgagac ggagatgcgc agcaaatacg cgctgcccgt cgcgcaagag cggtttggcg cgtttgccgc gcatgcgcgg gccggcggca tgtga

Details for BuceA.17622.a.B1.GE41152

HARVESTED ON:	3/1/2017
SEQUENCED ON:	3/2/2017
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMD QSQVFRAMMP GLTSASGARV TVAYPSFPRP LPLVRLDRRG LVFRAAGAAP LVCGLPRPAT LLVADEPLCS LRLVIRDIAA TGDGRHDLTM QPSGAAGDEL LWHALRDREP YRRIRHPLAP VDLPVPIDGE PHAAAGETAA RPSRSAAFRL PCHSDALFFA DWLEYHFDEL RALSQQHGPQ LQLNQLERQV DDDEVGVRFV YDIDGHATRH ALTDCAREAC AWIETEMRSK YALPVAQERF GAFAAHARAG GM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggacc agtctcaggt ctttcgcgcg atgatgcccg ggttgacgag cgccagcggc gcccgcgtca cggtcgccta tccgtcgttt cccaggccgt tgccgctcgt gcggctcgac cgccgcgggc tcgtgtttcg tgccgccggc gccgcaccgc tcgtgtgcgg gctgccgcgc ccggcaaccc tgctggtcgc ggacgagccg ctgtgctcgc tgcgcctcgt gatccgcgac atcgccgcga ccggcgacgg ccgccacgac ctgacgatgc agccgtccgg cgccgccggt gacgaattgc tgtggcacgc gttgcgcgat cgcgagccgt accggcgaat tcggcacccg ctcgcgccgg tcgatctgcc cgttcccatc gacggcgaac cgcatgcggc ggccggcgaa accgctgcgc gcccgtcgcg cagcgcggct ttccgcctgc cctgccacag cgacgcgctg ttcttcgcgg actggctcga atatcacttc gatgaactgc gcgcattgtc gcaacagcac gggccgcaac tgcagctcaa tcagctcgaa cgacaggttg acgatgacga ggtcggcgtg cgtttcgtct acgacatcga cgggcatgcg acgcgacacg cattgaccga ctgcgcgcgc gaggcatgtg catggatcga gacggagatg cgcagcaaat acgcgctgcc cgtcgcgcaa gagcggtttg gcgcgtttgc cgcgcatgcg cgggccggcg gcatgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat