BuceA.17460.a
HpcH/HpaI aldolase/citrate lyase family protein
| CENTER ID: | BuceA.17460.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.17460.a.B1.GE36634 | full length | 1 | 261 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.17460.a.B1.PS01858 | full length | 1 | 261 |
Structures
4MF4
DEPOSITED: 8/27/2013
DETERMINATION: XRay
CLONE: BuceA.17460.a.B1.GE36634
PROTEIN: BuceA.17460.a.B1.PS01858
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|216591.5.peg.496 |
| RefSeq: | YP_002229638.1 |
| UniProt: | B4E7W8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTLTNSLKQ RLRDGDEPLY GLWLSLGSDS AAEALAHAGY DWLCIDMEHA PNDSRDVASQ LRAIAAAHLP SEPVVRVPAR EPWLVKRALD AGARTLMFPC IETPDDAAHA VRLTRFPSPE SPDGLRGVAG MVRAAAFGMR RDYLQTANAQ VAVIVQVESA RGVDEVERIA ATPGVDCLFV GPADLAASLG HLGDIRHPDV ETAMARVLAA GKQAGVAVGI FAGDTAAARQ YREAGYRLIT VSADVSWLLR ATRQALQEVR S
NT Sequence
atgagcacgc tcaccaattc cctcaaacaa cgcctgcgcg acggcgacga gccgctgtac ggcctgtggc tgtcgctcgg cagcgactcg gccgcggaag cgctcgcgca tgccggttac gactggctct gcatcgacat ggagcatgcg ccgaacgaca gtcgcgatgt agcgtcgcaa ctgcgcgcga tcgccgcggc gcacctgccg agcgagccgg tcgtgcgcgt gccggcgcgc gagccgtggc tcgtgaagcg tgcgctcgac gccggcgccc gcacgctgat gtttccgtgt atcgagacgc ccgacgacgc cgcgcacgcg gtgcggctca cgcgcttccc gtcgcccgaa tcgccggacg ggctgcgcgg cgtcgcgggc atggtgcgcg cggcggcgtt cggcatgcgc cgcgactacc tgcagacggc gaatgcgcaa gtcgcggtga tcgtgcaggt cgaatcggcg cgcggcgtcg acgaagtcga gcggatcgcg gcgacgcccg gtgtcgattg cctgttcgtc ggcccggccg atctcgcggc gagcctcggg catctcggcg atatccgcca cccggacgtc gaaaccgcga tggcgcgcgt gctcgcggcg ggcaagcagg cgggcgtcgc ggtcggcatc ttcgcgggcg acacggcggc cgcgcgccag tatcgcgagg ccggctaccg gttgatcacg gtgtcggccg acgtgagctg gctgttgcgc gcgacgcggc aggcgttgca ggaggtacgg tcatga
Details for BuceA.17460.a.B1.GE36634
| HARVESTED ON: | 2/8/2013 |
| SEQUENCED ON: | 2/14/2013 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 79 |
Validated AA Sequence
MEHAPNDSRD VASQLRAIAA AHLPSEPVVR VPAREPWLVK RALDAGARTL MFPCIETPDD AAHAVRLTRF PSPESPDGLR GVAGMVRAAA FGMRRDYLQT ANAQVAVIVQ VESARGVDEV ERIAATPGVD CLFVGPADLA ASLGHLGDIR HPDVETAMAR VLAAGKQAGV AVGIFAGDTA AARQYREAGY RLITVSADVS WLLRATRQAL QEVRS
Validated NT Sequence
atcttactca cttatgaccg tacctcctgc aacgcctgcc gcgtcgcgcg caacagccag ctcacgtcgg ccgacaccgt gatcaaccgg tagccggcct cgcgatactg gcgcgcggcc gccgtgtcgc ccgcgaagat gccgaccgcg acgcccgcct gcttgcccgc cgcgagcacg cgcgccatcg cggtttcgac gtccgggtgg cggatatcgc cgagatgccc gaggctcgcc gcgagatcgg ccgggccgac gaacaggcaa tcgacaccgg gcgtcgccgc gatccgctcg acttcgtcga cgccgcgcgc cgattcgacc tgcacgatca ccgcgacttg cgcattcgcc gtctgcaggt agtcgcggcg catgccgaac gccgccgcgc gcaccatgcc cgcgacgccg cgcagcccgt ccggcgattc gggcgacggg aagcgcgtga gccgcaccgc gtgcgcggcg tcgtcgggcg tctcgataca cggaaacatc agcgtgcggg cgccggcgtc gagcgcacgc ttcacgagcc acggctcgcg cgccggcacg cgcacgaccg gctcgctcgg caggtgcgcc gcggcgatcg cgcgcagttg cgacgctaca tcgcgactgt cgttcggcgc atgctccatg tcgatgcaga gccagtcgta accggcatgc gcgagcgctt ccgcggccga gtcgctgccg agcgacagcc acaggccgta cagcggctcg tcgccgtcgc gcaggcgttg tttgagggaa ttggtgagcg tg
Expected Protein Sequence
MAHHHHHHMS TLTNSLKQRL RDGDEPLYGL WLSLGSDSAA EALAHAGYDW LCIDMEHAPN DSRDVASQLR AIAAAHLPSE PVVRVPAREP WLVKRALDAG ARTLMFPCIE TPDDAAHAVR LTRFPSPESP DGLRGVAGMV RAAAFGMRRD YLQTANAQVA VIVQVESARG VDEVERIAAT PGVDCLFVGP ADLAASLGHL GDIRHPDVET AMARVLAAGK QAGVAVGIFA GDTAAARQYR EAGYRLITVS ADVSWLLRAT RQALQEVRS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca cgctcaccaa ttccctcaaa caacgcctgc gcgacggcga cgagccgctg tacggcctgt ggctgtcgct cggcagcgac tcggccgcgg aagcgctcgc gcatgccggt tacgactggc tctgcatcga catggagcat gcgccgaacg acagtcgcga tgtagcgtcg caactgcgcg cgatcgccgc ggcgcacctg ccgagcgagc cggtcgtgcg cgtgccggcg cgcgagccgt ggctcgtgaa gcgtgcgctc gacgccggcg cccgcacgct gatgtttccg tgtatcgaga cgcccgacga cgccgcgcac gcggtgcggc tcacgcgctt cccgtcgccc gaatcgccgg acgggctgcg cggcgtcgcg ggcatggtgc gcgcggcggc gttcggcatg cgccgcgact acctgcagac ggcgaatgcg caagtcgcgg tgatcgtgca ggtcgaatcg gcgcgcggcg tcgacgaagt cgagcggatc gcggcgacgc ccggtgtcga ttgcctgttc gtcggcccgg ccgatctcgc ggcgagcctc gggcatctcg gcgatatccg ccacccggac gtcgaaaccg cgatggcgcg cgtgctcgcg gcgggcaagc aggcgggcgt cgcggtcggc atcttcgcgg gcgacacggc ggccgcgcgc cagtatcgcg aggccggcta ccggttgatc acggtgtcgg ccgacgtgag ctggctgttg cgcgcgacgc ggcaggcgtt gcaggaggta cggtcatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BuceA.17460.a.B1.PS01858
| PURIFICATION DATe: | 7/9/2013 |
| CONCENTRATION: | 65.1mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 79 |
Protocol Notes
notes unavailable
Validated AA Sequence
MEHAPNDSRD VASQLRAIAA AHLPSEPVVR VPAREPWLVK RALDAGARTL MFPCIETPDD AAHAVRLTRF PSPESPDGLR GVAGMVRAAA FGMRRDYLQT ANAQVAVIVQ VESARGVDEV ERIAATPGVD CLFVGPADLA ASLGHLGDIR HPDVETAMAR VLAAGKQAGV AVGIFAGDTA AARQYREAGY RLITVSADVS WLLRATRQAL QEVRS
Validated NT Sequence
atcttactca cttatgaccg tacctcctgc aacgcctgcc gcgtcgcgcg caacagccag ctcacgtcgg ccgacaccgt gatcaaccgg tagccggcct cgcgatactg gcgcgcggcc gccgtgtcgc ccgcgaagat gccgaccgcg acgcccgcct gcttgcccgc cgcgagcacg cgcgccatcg cggtttcgac gtccgggtgg cggatatcgc cgagatgccc gaggctcgcc gcgagatcgg ccgggccgac gaacaggcaa tcgacaccgg gcgtcgccgc gatccgctcg acttcgtcga cgccgcgcgc cgattcgacc tgcacgatca ccgcgacttg cgcattcgcc gtctgcaggt agtcgcggcg catgccgaac gccgccgcgc gcaccatgcc cgcgacgccg cgcagcccgt ccggcgattc gggcgacggg aagcgcgtga gccgcaccgc gtgcgcggcg tcgtcgggcg tctcgataca cggaaacatc agcgtgcggg cgccggcgtc gagcgcacgc ttcacgagcc acggctcgcg cgccggcacg cgcacgaccg gctcgctcgg caggtgcgcc gcggcgatcg cgcgcagttg cgacgctaca tcgcgactgt cgttcggcgc atgctccatg tcgatgcaga gccagtcgta accggcatgc gcgagcgctt ccgcggccga gtcgctgccg agcgacagcc acaggccgta cagcggctcg tcgccgtcgc gcaggcgttg tttgagggaa ttggtgagcg tg
Expressed Protein Sequence
MAHHHHHHMS TLTNSLKQRL RDGDEPLYGL WLSLGSDSAA EALAHAGYDW LCIDMEHAPN DSRDVASQLR AIAAAHLPSE PVVRVPAREP WLVKRALDAG ARTLMFPCIE TPDDAAHAVR LTRFPSPESP DGLRGVAGMV RAAAFGMRRD YLQTANAQVA VIVQVESARG VDEVERIAAT PGVDCLFVGP ADLAASLGHL GDIRHPDVET AMARVLAAGK QAGVAVGIFA GDTAAARQYR EAGYRLITVS ADVSWLLRAT RQALQEVRS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA CGCTCACCAA TTCCCTCAAA CAACGCCTGC GCGACGGCGA CGAGCCGCTG TACGGCCTGT GGCTGTCGCT CGGCAGCGAC TCGGCCGCGG AAGCGCTCGC GCATGCCGGT TACGACTGGC TCTGCATCGA CATGGAGCAT GCGCCGAACG ACAGTCGCGA TGTAGCGTCG CAACTGCGCG CGATCGCCGC GGCGCACCTG CCGAGCGAGC CGGTCGTGCG CGTGCCGGCG CGCGAGCCGT GGCTCGTGAA GCGTGCGCTC GACGCCGGCG CCCGCACGCT GATGTTTCCG TGTATCGAGA CGCCCGACGA CGCCGCGCAC GCGGTGCGGC TCACGCGCTT CCCGTCGCCC GAATCGCCGG ACGGGCTGCG CGGCGTCGCG GGCATGGTGC GCGCGGCGGC GTTCGGCATG CGCCGCGACT ACCTGCAGAC GGCGAATGCG CAAGTCGCGG TGATCGTGCA GGTCGAATCG GCGCGCGGCG TCGACGAAGT CGAGCGGATC GCGGCGACGC CCGGTGTCGA TTGCCTGTTC GTCGGCCCGG CCGATCTCGC GGCGAGCCTC GGGCATCTCG GCGATATCCG CCACCCGGAC GTCGAAACCG CGATGGCGCG CGTGCTCGCG GCGGGCAAGC AGGCGGGCGT CGCGGTCGGC ATCTTCGCGG GCGACACGGC GGCCGCGCGC CAGTATCGCG AGGCCGGCTA CCGGTTGATC ACGGTGTCGG CCGACGTGAG CTGGCTGTTG CGCGCGACGC GGCAGGCGTT GCAGGAGGTA CGGTCATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T