BuceA.17449.a

Putative uncharacterized protein

CENTER ID: BuceA.17449.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.17449.a.B1.GE35726 full length 1 294
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1104
RefSeq: YP_002230200.1
UniProt: B4ECL4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATQRAARAL IFTADDFGLH PRVNAAVERA HRDGVLNAAS LMVGASAAQD AIERARRLPS LAVGLHLVLA DGPATLPAHE IPALVGRDGR FGDAMAKDGC RFFFLPHVRA QLRREIRAQF DAFAASGLPL DHVNAHKHFH LHPTVLSMII EIGRDYGLRA VRLPYETSAP ALLKPWIALV RARLDRAGLA HNDYVVGIEH TGAMDEAVLL DALAKLPAGV GEIYCHPAEA GDGPITPTMA DYRPVDELEA LLSPRVAAAL KAAGVATGGF ADVFGGPAAR RGAQASRAPG AQPS
NT Sequence
gtggcgacgc agcgggcggc gcgggcgctg atcttcaccg cggacgactt cgggctgcac ccgcgcgtca acgcggcggt cgagcgtgcg caccgcgacg gcgtgctgaa cgccgccagc ctgatggtcg gcgcgagcgc cgcgcaggat gcgatcgaac gcgcgcggcg gctgccgtcg ctcgcggtcg gcctgcatct ggtgctcgcg gacgggccgg ccacgctgcc cgcgcatgag attcccgcgc tcgtcggccg cgacgggcga ttcggcgacg cgatggcgaa ggacggctgt cgcttcttct tcctgccgca cgtgcgcgcg caactgcgcc gcgagatccg tgcgcagttc gatgcgttcg cggcgagcgg cctgccgctc gaccacgtga acgcgcacaa gcatttccac ctgcatccga ccgtgctgtc gatgatcatc gagatcggcc gcgactacgg gctgcgcgcg gtgcggctgc cgtacgaaac gagcgcgccc gcgctgctca agccgtggat cgcgctggtg cgcgcgcggc tcgaccgcgc ggggctcgcg cacaacgact acgtggtcgg gatcgagcat acgggcgcga tggacgaggc cgtgctgctc gacgcgctcg cgaagctgcc ggcgggcgtc ggcgagatct actgtcatcc ggccgaggcc ggcgacggcc cgatcacgcc gacgatggcc gattaccgtc cggtcgacga gctcgaggcg ctgctgtcgc cgcgcgtcgc ggccgcgctg aaggcggcgg gcgtcgcgac cggcgggttt gccgacgtgt tcggcgggcc ggcggcgcga cgcggcgcgc aggcgtcgcg cgcaccggga gcgcagccgt catga
Details for BuceA.17449.a.B1.GE35726
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/3/2012
EXPECTED MW: 32kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 19
PERCENT COVERAGE: 77
Validated AA Sequence
MTVDXADARR QLRERVXQHG LVHRARMLDP DHVVVVREPR AVEPRAHQRD XXLEQRGRAR FVRQPHRAQP VVAADLDDHR QXXXXQVEXL VRVXVVEXQX ARREXIXXXX DLXXQLRXHG XXKKKXQPSX AIXXXXXXXX XXXXGXSXXX XXXGXSAXXR CXXXXXXGSP RXXDXIXXGA XADHQXXAXX MPSRXARSXX XXRXXXXXSX PXXXPXXXRX XHXXXXVXSH GISPS
Validated NT Sequence
cnnnnnnntt ttgtnnnntt taagaaggag atataccatg gctcancacc nncnccancn tatggngncn acgcncgngn ncnggnnttn acnncggacn acttcnggnt gnancnncnc gncncgnngn ngncgancgt gcgcnccgcg acggcatgnt naacgccncc ngctgatggt cggcgcnagc nccnnnnang atgcnatcna anncncgcgg gctgccntcn ctcnnggnnn ncntgcatct ggngntcgcg gacnggccgg ncncnctgnc nncncatgan attcccncgn tcgncngnnn nnacnggnga nncnncnacn cgatggcnaa ngacggctgn nncttcttct tcntgncncc gtgcgngcgc aactgcnccn cgagatccan gnncanntcg atgcnttcgc ggcgagcggn ctgncnctcg accacntgaa cgcgcacnag cntttccacc tgcntccnan gntgctgtcg atgatcatcg agatcggccg cgactacggg ctgcgcgcgg tgcggctgcc gtacgaaacg agcgcgcccg cgctgctcaa gncnngnatc gcgctggtgc gcgcgcggct cgaccgcgcg gggctcgcgc acaacgacta cgtggtcggg atcgagcata cgggcgcgat ggacnaggcc gtgctgcnnn acgcgctcgc gaagctgccg gcgggcgtcg gcganatcta ctgtcatccn ggccgangcc ggngacggnc cgatcacncc gacgatggcc gantaccgtc cggtcnacna gctnnnnggc nnntgctgtc gccgcgcgtc ncngnnnnnn nnnannnnnn nng
Expected Protein Sequence
MAHHHHHHMV ATQRAARALI FTADDFGLHP RVNAAVERAH RDGVLNAASL MVGASAAQDA IERARRLPSL AVGLHLVLAD GPATLPAHEI PALVGRDGRF GDAMAKDGCR FFFLPHVRAQ LRREIRAQFD AFAASGLPLD HVNAHKHFHL HPTVLSMIIE IGRDYGLRAV RLPYETSAPA LLKPWIALVR ARLDRAGLAH NDYVVGIEHT GAMDEAVLLD ALAKLPAGVG EIYCHPAEAG DGPITPTMAD YRPVDELEAL LSPRVAAALK AAGVATGGFA DVFGGPAARR GAQASRAPGA QPS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgg cgacgcagcg ggcggcgcgg gcgctgatct tcaccgcgga cgacttcggg ctgcacccgc gcgtcaacgc ggcggtcgag cgtgcgcacc gcgacggcgt gctgaacgcc gccagcctga tggtcggcgc gagcgccgcg caggatgcga tcgaacgcgc gcggcggctg ccgtcgctcg cggtcggcct gcatctggtg ctcgcggacg ggccggccac gctgcccgcg catgagattc ccgcgctcgt cggccgcgac gggcgattcg gcgacgcgat ggcgaaggac ggctgtcgct tcttcttcct gccgcacgtg cgcgcgcaac tgcgccgcga gatccgtgcg cagttcgatg cgttcgcggc gagcggcctg ccgctcgacc acgtgaacgc gcacaagcat ttccacctgc atccgaccgt gctgtcgatg atcatcgaga tcggccgcga ctacgggctg cgcgcggtgc ggctgccgta cgaaacgagc gcgcccgcgc tgctcaagcc gtggatcgcg ctggtgcgcg cgcggctcga ccgcgcgggg ctcgcgcaca acgactacgt ggtcgggatc gagcatacgg gcgcgatgga cgaggccgtg ctgctcgacg cgctcgcgaa gctgccggcg ggcgtcggcg agatctactg tcatccggcc gaggccggcg acggcccgat cacgccgacg atggccgatt accgtccggt cgacgagctc gaggcgctgc tgtcgccgcg cgtcgcggcc gcgctgaagg cggcgggcgt cgcgaccggc gggtttgccg acgtgttcgg cgggccggcg gcgcgacgcg gcgcgcaggc gtcgcgcgca ccgggagcgc agccgtcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat