SSGCID: Target Page

BuceA.17389.a

L-arabinose formyltransferase

CENTER ID:	BuceA.17389.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17389.a.B1.GE36633	full length		1	315

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17389.a.B1.PW37293	full length		1	315

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2007
RefSeq:	YP_002231061.1
UniProt:	B4EB19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKPRAVVFAY HNVGVRCLQV LLARGVDVAL VVTHEDNPNE NIWFGSVASV AAEHGIPVVT PADPADPALR RAVADAQPDF IFSFYYRHML PVDLLAIAPR GAYNMHGSLL PKYRGRVPTN WAVLNGETET GATLHEMAAK PDAGAIIGQT AVPILPDDTA AQVFDKVTVA AEQTLWRVLP ALLAGEASHL PNDLATGSYY GGRKPEDGRV DWSKPAAQVY NLVRAVAPPY PGAFTDIHGA RFVIARARLA APGSAAAAAA ADLPPGLHVS DNALFGVCGD SRALSILELR QQRDGSETVV TPAEFAQFIH SSRHS

NT Sequence

atgaagccgc gcgcggtcgt cttcgcatat cacaacgtcg gcgtgcggtg cctgcaggtg ctgctcgcgc gcggcgtcga cgtcgcgctg gtcgtcacgc acgaggacaa cccgaacgag aacatctggt tcggcagcgt cgcgtccgtc gcggccgagc acggcattcc ggtcgtcacg ccggccgacc cggccgatcc ggcgctgcgc cgcgcggtgg ccgacgcgca gcccgatttc atcttctcgt tctactaccg ccacatgctg ccggtggacc tgctcgcgat cgcgccgcgc ggcgcgtaca acatgcacgg ttcgctgctg ccgaaatacc ggggtcgggt accgaccaac tgggccgtgc tgaacggcga gaccgaaacc ggcgcgacgc tgcacgagat ggccgccaag ccggacgcgg gcgcgatcat cggccagacc gcggtgccga tcctgccgga cgacacggcc gcgcaggtgt tcgacaaggt cacggtggcc gccgagcaga cgctgtggcg cgtgctgccc gcgctgctcg cgggcgaggc gtcgcacctg ccgaacgatc tcgcgaccgg cagctactac ggcgggcgca agcccgagga cggccgcgtc gactggtcga agccggccgc gcaggtctac aacctggtgc gcgcggtcgc gcccccgtat ccgggcgcat tcacggacat ccacggcgcc cggttcgtga tcgcgcgcgc gcgcctcgcg gcgcccggca gcgcggcagc ggccgccgcg gcagatttgc cgcccggcct gcacgtaagc gataatgcgc tattcggcgt ctgcggcgac agccgcgccc tatccattct cgagctccgg cagcagcgcg acggcagcga aaccgtcgtg acgcccgcgg aattcgcgca gttcattcat tcttcccgtc attcatga

Details for BuceA.17389.a.B1.GE36633

HARVESTED ON:	2/8/2013
SEQUENCED ON:	2/14/2013
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK PRAVVFAYHN VGVRCLQVLL ARGVDVALVV THEDNPNENI WFGSVASVAA EHGIPVVTPA DPADPALRRA VADAQPDFIF SFYYRHMLPV DLLAIAPRGA YNMHGSLLPK YRGRVPTNWA VLNGETETGA TLHEMAAKPD AGAIIGQTAV PILPDDTAAQ VFDKVTVAAE QTLWRVLPAL LAGEASHLPN DLATGSYYGG RKPEDGRVDW SKPAAQVYNL VRAVAPPYPG AFTDIHGARF VIARARLAAP GSAAAAAAAD LPPGLHVSDN ALFGVCGDSR ALSILELRQQ RDGSETVVTP AEFAQFIHSS RHS

Validated NT Sequence

tactcactta tgaatgacgg gaagaatgaa tgaactgcgc gaattccgcg ggcgtcacga cggtttcgct gccgtcgcgc tgctgccgga gctcgagaat ggatagggcg cggctgtcgc cgcagacgcc gaatagcgca ttatcgctta cgtgcaggcc gggcggcaaa tctgccgcgg cggccgctgc cgcgctgccg ggcgccgcga ggcgcgcgcg cgcgatcacg aaccgggcgc cgtggatgtc cgtgaatgcg cccggatacg ggggcgcgac cgcgcgcacc aggttgtaga cctgcgcggc cggcttcgac cagtcgacgc ggccgtcctc gggcttgcgc ccgccgtagt agctgccggt cgcgagatcg ttcggcaggt gcgacgcctc gcccgcgagc agcgcgggca gcacgcgcca cagcgtctgc tcggcggcca ccgtgacctt gtcgaacacc tgcgcggccg tgtcgtccgg caggatcggc accgcggtct ggccgatgat cgcgcccgcg tccggcttgg cggccatctc gtgcagcgtc gcgccggttt cggtctcgcc gttcagcacg gcccagttgg tcggtacccg accccggtat ttcggcagca gcgaaccgtg catgttgtac gcgccgcgcg gcgcgatcgc gagcaggtcc accggcagca tgtggcggta gtagaacgag aagatgaaat cgggctgcgc gtcggccacc gcgcggcgca gcgccggatc ggccgggtcg gccggcgtga cgaccggaat gccgtgctcg gccgcgacgg acgcgacgct gccgaaccag atgttctcgt tcgggttgtc ctcgtgcgtg acgaccagcg cgacgtcgac gccgcgcgcg agcagcacct gcaggcaccg cacgccgacg ttgtgatatg cgaagacgac cgcgcgcggc ttcatatggt ggtggtggtg gtgagccatg gtat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc cgcgcgcggt cgtcttcgca tatcacaacg tcggcgtgcg gtgcctgcag gtgctgctcg cgcgcggcgt cgacgtcgcg ctggtcgtca cgcacgagga caacccgaac gagaacatct ggttcggcag cgtcgcgtcc gtcgcggccg agcacggcat tccggtcgtc acgccggccg acccggccga tccggcgctg cgccgcgcgg tggccgacgc gcagcccgat ttcatcttct cgttctacta ccgccacatg ctgccggtgg acctgctcgc gatcgcgccg cgcggcgcgt acaacatgca cggttcgctg ctgccgaaat accggggtcg ggtaccgacc aactgggccg tgctgaacgg cgagaccgaa accggcgcga cgctgcacga gatggccgcc aagccggacg cgggcgcgat catcggccag accgcggtgc cgatcctgcc ggacgacacg gccgcgcagg tgttcgacaa ggtcacggtg gccgccgagc agacgctgtg gcgcgtgctg cccgcgctgc tcgcgggcga ggcgtcgcac ctgccgaacg atctcgcgac cggcagctac tacggcgggc gcaagcccga ggacggccgc gtcgactggt cgaagccggc cgcgcaggtc tacaacctgg tgcgcgcggt cgcgcccccg tatccgggcg cattcacgga catccacggc gcccggttcg tgatcgcgcg cgcgcgcctc gcggcgcccg gcagcgcggc agcggccgcc gcggcagatt tgccgcccgg cctgcacgta agcgataatg cgctattcgg cgtctgcggc gacagccgcg ccctatccat tctcgagctc cggcagcagc gcgacggcag cgaaaccgtc gtgacgcccg cggaattcgc gcagttcatt cattcttccc gtcattcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for BuceA.17389.a.B1.PW37293

PURIFICATION DATe:	5/22/2013
CONCENTRATION:	30.4mg/ml
OBSERVED MW:	33kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGC CGCGCGCGGT CGTCTTCGCA TATCACAACG TCGGCGTGCG GTGCCTGCAG GTGCTGCTCG CGCGCGGCGT CGACGTCGCG CTGGTCGTCA CGCACGAGGA CAACCCGAAC GAGAACATCT GGTTCGGCAG CGTCGCGTCC GTCGCGGCCG AGCACGGCAT TCCGGTCGTC ACGCCGGCCG ACCCGGCCGA TCCGGCGCTG CGCCGCGCGG TGGCCGACGC GCAGCCCGAT TTCATCTTCT CGTTCTACTA CCGCCACATG CTGCCGGTGG ACCTGCTCGC GATCGCGCCG CGCGGCGCGT ACAACATGCA CGGTTCGCTG CTGCCGAAAT ACCGGGGTCG GGTACCGACC AACTGGGCCG TGCTGAACGG CGAGACCGAA ACCGGCGCGA CGCTGCACGA GATGGCCGCC AAGCCGGACG CGGGCGCGAT CATCGGCCAG ACCGCGGTGC CGATCCTGCC GGACGACACG GCCGCGCAGG TGTTCGACAA GGTCACGGTG GCCGCCGAGC AGACGCTGTG GCGCGTGCTG CCCGCGCTGC TCGCGGGCGA GGCGTCGCAC CTGCCGAACG ATCTCGCGAC CGGCAGCTAC TACGGCGGGC GCAAGCCCGA GGACGGCCGC GTCGACTGGT CGAAGCCGGC CGCGCAGGTC TACAACCTGG TGCGCGCGGT CGCGCCCCCG TATCCGGGCG CATTCACGGA CATCCACGGC GCCCGGTTCG TGATCGCGCG CGCGCGCCTC GCGGCGCCCG GCAGCGCGGC AGCGGCCGCC GCGGCAGATT TGCCGCCCGG CCTGCACGTA AGCGATAATG CGCTATTCGG CGTCTGCGGC GACAGCCGCG CCCTATCCAT TCTCGAGCTC CGGCAGCAGC GCGACGGCAG CGAAACCGTC GTGACGCCCG CGGAATTCGC GCAGTTCATT CATTCTTCCC GTCATTCATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT