BuceA.17333.a
Putative UDP-4-amino-4-deoxy-l-arabinose--oxoglutarate aminotransferas
| CENTER ID: | BuceA.17333.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.17333.a.B1.GE36632 | full length | 1 | 383 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.17333.a.B1.PS01806 | full length | 1 | 383 |
Structures
4LC3
DEPOSITED: 6/21/2013
DETERMINATION: XRay
CLONE: BuceA.17333.a.B1.GE36632
PROTEIN: BuceA.17333.a.B1.PS01806
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|216591.5.peg.2005 |
| RefSeq: | YP_002231059.1 |
| UniProt: | B4EB17 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQTTAPFLP FTRPEIDEET IQGVVEVLRS GWITTGPQCQ KFEAALSEYC GGRPVRVFNS GTCTLEIGLR IAGVGPGDEV ITTPASWVST SNVIIETGAT PVFADIDPVT RNIDLDKLEQ AITPRTKAII PVFLSGLPVD MDRLYAIARA HKLRVIEDAA QAFGSTWHGK RIGAIGDLVS FSFHANKNLT TIEGGALVLN NEDEAVLAQK YRLQGITRTG FDGMDCDVLG GKYNLTDVAA RVGLGQLPHL ERFTAQRRAL ARAYFAAFDG GAAAKLGVGL PVAEFENGNW HMFLVTLPLE RLTITRAEFM AQMKERGIGT GIHYPAIHLF TLYRARGFKE GMFPHAERYG ASTVTLPLFT QMTEGDVRRV VDAVNQICEQ YGK
NT Sequence
atgagccaga ctaccgcccc gtttctgccg ttcacccgcc ccgagatcga cgaggaaacc atccagggcg tcgtcgaggt gctgcgctcg ggctggatca ccaccggccc gcagtgccag aaattcgagg cggcactgtc cgagtactgc ggcggccgtc cggtccgcgt attcaattcg ggcacctgca cgctcgagat cggcctgcgc atcgcgggcg tcggccccgg cgacgaggtg atcacgacgc cggcgtcgtg ggtctcgacc agcaacgtga tcatcgaaac gggcgcgacg cccgtgttcg cggacatcga tcccgtcacg cgcaacatcg acctcgacaa gctcgagcag gcgatcacgc cgcgcacgaa ggcgatcatc cccgtgttcc tgtccggcct gccggtcgac atggaccgcc tgtacgcgat cgcccgcgcg cacaagctgc gcgtgatcga ggatgccgcg caggcgttcg gctcgacgtg gcacggcaag cgcatcggcg cgatcggcga cctcgtgtcg ttcagcttcc acgcgaacaa gaacctgacg acgatcgaag gcggcgcgct cgtgctgaac aacgaggacg aagcggtgct cgcgcagaag taccggctgc agggcatcac gcgcaccggc ttcgacggaa tggactgcga cgtgctcggc ggcaagtaca acctgaccga cgtcgccgcg cgcgtcggcc tcggccagct gccgcacctc gagcgcttca ccgcgcagcg ccgcgcactc gcgcgcgcgt atttcgccgc gttcgacggc ggcgcggccg cgaagctcgg cgtcggcctg cccgtcgccg aattcgagaa cggcaactgg cacatgttcc tggtcacgct gccgctcgag cggctgacga tcacgcgcgc cgaattcatg gcgcagatga aggaacgcgg catcggcacg ggcatccact acccggcgat ccatcttttc acgctgtacc gtgcgcgcgg gttcaaggag ggcatgttcc cccacgccga acggtacggc gcgtcgaccg tcacgctgcc gctgtttacg cagatgacgg aaggcgacgt gcgtcgcgtc gtcgacgccg tcaatcagat ttgcgaacaa tacggaaaat aa
Details for BuceA.17333.a.B1.GE36632
| HARVESTED ON: | 2/8/2013 |
| SEQUENCED ON: | 2/15/2013 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MSQTTAPFLP FTRPEIDEET IQGVVEVLRS GWITTGPQCQ KFEAALSEYC GGRPVRVFNS GTCTLEIGLR IAGVGPGDEV ITTPASWVST SNVIIETGAT PVFADIDPVT RNIDLDKLEQ AITPRTKAII PVFLSGLPVD MDRLYAIARA HKLRVIEDAA QAFGSTWHGK RIGAIGDLVS FSFHANKNLT TIEGGALVLN NEDEAVLAQK YRLQGITRTG FDGMDCDVLG GKYNLTDVAA RVGLGQLPHL ERFTAQRRAL ARAYFAAFDG GAAAKLGVGL PVAEFENGNW HMFLVTLPLE RLTITRAEFM AQMKERGIGT GIHYPAIHLF TLYRARGFKE GMFPHAERYG ASTVTLPLFT QMTEGDVRRV VDAVNQICEQ YGK
Validated NT Sequence
ccatatgagc cagactaccg ccccgtttct gccgttcacc cgccccgaga tcgacgagga aaccatccag ggcgtcgtcg aggtgctgcg ctcgggctgg atcaccaccg gcccgcagtg ccagaaattc gaggcggcac tgtccgagta ctgcggcggc cgtccggtcc gcgtattcaa ttcgggcacc tgcacgctcg agatcggcct gcgcatcgcg ggcgtcggcc ccggcgacga ggtgatcacg acgccggcgt cgtgggtctc gaccagcaac gtgatcatcg aaacgggcgc gacgcccgtg ttcgcggaca tcgatcccgt cacgcgcaac atcgacctcg acaagctcga gcaggcgatc acgccgcgca cgaaggcgat catccccgtg ttcctgtccg gcctgccggt cgacatggac cgcctgtacg cgatcgcccg cgcgcacaag ctgcgcgtga tcgaggatgc cgcgcaggcg ttcggctcga cgtggcacgg caagcgcatc ggcgcgatcg gcgacctcgt gtcgttcagc ttccacgcga acaagaacct gacgacgatc gaaggcggcg cgctcgtgct gaacaacgag gacgaagcgg tgctcgcgca gaagtaccgg ctgcagggca tcacgcgcac cggcttcgac ggaatggact gcgacgtgct cggcggcaag tacaacctga ccgacgtcgc cgcgcgcgtc ggcctcggcc agctgccgca cctcgagcgc ttcaccgcgc agcgccgcgc actcgcgcgc gcgtatttcg ccgcgttcga cggcggcgcg gccgcgaagc tcggcgtcgg cctgcccgtc gccgaattcg agaacggcaa ctggcacatg ttcctggtca cgctgccgct cgagcggctg acgatcacgc gcgccgaatt catggcgcag atgaaggaac gcggcatcgg cacgggcatc cactacccgg cgatccatct tttcacgctg taccgtgcgc gcgggttcaa ggagggcatg ttcccccacg ccgaacggta cggcgcgtcg accgtcacgc tgccgctgtt tacgcagatg acggaaggcg acgtgcgtcg cgtcgtcgac gccgtcaatc agatttgcga acaatacgga aaataagtga gtaagat
Expected Protein Sequence
MAHHHHHHMS QTTAPFLPFT RPEIDEETIQ GVVEVLRSGW ITTGPQCQKF EAALSEYCGG RPVRVFNSGT CTLEIGLRIA GVGPGDEVIT TPASWVSTSN VIIETGATPV FADIDPVTRN IDLDKLEQAI TPRTKAIIPV FLSGLPVDMD RLYAIARAHK LRVIEDAAQA FGSTWHGKRI GAIGDLVSFS FHANKNLTTI EGGALVLNNE DEAVLAQKYR LQGITRTGFD GMDCDVLGGK YNLTDVAARV GLGQLPHLER FTAQRRALAR AYFAAFDGGA AAKLGVGLPV AEFENGNWHM FLVTLPLERL TITRAEFMAQ MKERGIGTGI HYPAIHLFTL YRARGFKEGM FPHAERYGAS TVTLPLFTQM TEGDVRRVVD AVNQICEQYG K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc agactaccgc cccgtttctg ccgttcaccc gccccgagat cgacgaggaa accatccagg gcgtcgtcga ggtgctgcgc tcgggctgga tcaccaccgg cccgcagtgc cagaaattcg aggcggcact gtccgagtac tgcggcggcc gtccggtccg cgtattcaat tcgggcacct gcacgctcga gatcggcctg cgcatcgcgg gcgtcggccc cggcgacgag gtgatcacga cgccggcgtc gtgggtctcg accagcaacg tgatcatcga aacgggcgcg acgcccgtgt tcgcggacat cgatcccgtc acgcgcaaca tcgacctcga caagctcgag caggcgatca cgccgcgcac gaaggcgatc atccccgtgt tcctgtccgg cctgccggtc gacatggacc gcctgtacgc gatcgcccgc gcgcacaagc tgcgcgtgat cgaggatgcc gcgcaggcgt tcggctcgac gtggcacggc aagcgcatcg gcgcgatcgg cgacctcgtg tcgttcagct tccacgcgaa caagaacctg acgacgatcg aaggcggcgc gctcgtgctg aacaacgagg acgaagcggt gctcgcgcag aagtaccggc tgcagggcat cacgcgcacc ggcttcgacg gaatggactg cgacgtgctc ggcggcaagt acaacctgac cgacgtcgcc gcgcgcgtcg gcctcggcca gctgccgcac ctcgagcgct tcaccgcgca gcgccgcgca ctcgcgcgcg cgtatttcgc cgcgttcgac ggcggcgcgg ccgcgaagct cggcgtcggc ctgcccgtcg ccgaattcga gaacggcaac tggcacatgt tcctggtcac gctgccgctc gagcggctga cgatcacgcg cgccgaattc atggcgcaga tgaaggaacg cggcatcggc acgggcatcc actacccggc gatccatctt ttcacgctgt accgtgcgcg cgggttcaag gagggcatgt tcccccacgc cgaacggtac ggcgcgtcga ccgtcacgct gccgctgttt acgcagatga cggaaggcga cgtgcgtcgc gtcgtcgacg ccgtcaatca gatttgcgaa caatacggaa aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuceA.17333.a.B1.PS01806
| PURIFICATION DATe: | 4/23/2013 |
| CONCENTRATION: | 50mg/ml |
| OBSERVED MW: | 38kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSQTTAPFLP FTRPEIDEET IQGVVEVLRS GWITTGPQCQ KFEAALSEYC GGRPVRVFNS GTCTLEIGLR IAGVGPGDEV ITTPASWVST SNVIIETGAT PVFADIDPVT RNIDLDKLEQ AITPRTKAII PVFLSGLPVD MDRLYAIARA HKLRVIEDAA QAFGSTWHGK RIGAIGDLVS FSFHANKNLT TIEGGALVLN NEDEAVLAQK YRLQGITRTG FDGMDCDVLG GKYNLTDVAA RVGLGQLPHL ERFTAQRRAL ARAYFAAFDG GAAAKLGVGL PVAEFENGNW HMFLVTLPLE RLTITRAEFM AQMKERGIGT GIHYPAIHLF TLYRARGFKE GMFPHAERYG ASTVTLPLFT QMTEGDVRRV VDAVNQICEQ YGK
Validated NT Sequence
ccatatgagc cagactaccg ccccgtttct gccgttcacc cgccccgaga tcgacgagga aaccatccag ggcgtcgtcg aggtgctgcg ctcgggctgg atcaccaccg gcccgcagtg ccagaaattc gaggcggcac tgtccgagta ctgcggcggc cgtccggtcc gcgtattcaa ttcgggcacc tgcacgctcg agatcggcct gcgcatcgcg ggcgtcggcc ccggcgacga ggtgatcacg acgccggcgt cgtgggtctc gaccagcaac gtgatcatcg aaacgggcgc gacgcccgtg ttcgcggaca tcgatcccgt cacgcgcaac atcgacctcg acaagctcga gcaggcgatc acgccgcgca cgaaggcgat catccccgtg ttcctgtccg gcctgccggt cgacatggac cgcctgtacg cgatcgcccg cgcgcacaag ctgcgcgtga tcgaggatgc cgcgcaggcg ttcggctcga cgtggcacgg caagcgcatc ggcgcgatcg gcgacctcgt gtcgttcagc ttccacgcga acaagaacct gacgacgatc gaaggcggcg cgctcgtgct gaacaacgag gacgaagcgg tgctcgcgca gaagtaccgg ctgcagggca tcacgcgcac cggcttcgac ggaatggact gcgacgtgct cggcggcaag tacaacctga ccgacgtcgc cgcgcgcgtc ggcctcggcc agctgccgca cctcgagcgc ttcaccgcgc agcgccgcgc actcgcgcgc gcgtatttcg ccgcgttcga cggcggcgcg gccgcgaagc tcggcgtcgg cctgcccgtc gccgaattcg agaacggcaa ctggcacatg ttcctggtca cgctgccgct cgagcggctg acgatcacgc gcgccgaatt catggcgcag atgaaggaac gcggcatcgg cacgggcatc cactacccgg cgatccatct tttcacgctg taccgtgcgc gcgggttcaa ggagggcatg ttcccccacg ccgaacggta cggcgcgtcg accgtcacgc tgccgctgtt tacgcagatg acggaaggcg acgtgcgtcg cgtcgtcgac gccgtcaatc agatttgcga acaatacgga aaataagtga gtaagat
Expressed Protein Sequence
MAHHHHHHMS QTTAPFLPFT RPEIDEETIQ GVVEVLRSGW ITTGPQCQKF EAALSEYCGG RPVRVFNSGT CTLEIGLRIA GVGPGDEVIT TPASWVSTSN VIIETGATPV FADIDPVTRN IDLDKLEQAI TPRTKAIIPV FLSGLPVDMD RLYAIARAHK LRVIEDAAQA FGSTWHGKRI GAIGDLVSFS FHANKNLTTI EGGALVLNNE DEAVLAQKYR LQGITRTGFD GMDCDVLGGK YNLTDVAARV GLGQLPHLER FTAQRRALAR AYFAAFDGGA AAKLGVGLPV AEFENGNWHM FLVTLPLERL TITRAEFMAQ MKERGIGTGI HYPAIHLFTL YRARGFKEGM FPHAERYGAS TVTLPLFTQM TEGDVRRVVD AVNQICEQYG K
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCC AGACTACCGC CCCGTTTCTG CCGTTCACCC GCCCCGAGAT CGACGAGGAA ACCATCCAGG GCGTCGTCGA GGTGCTGCGC TCGGGCTGGA TCACCACCGG CCCGCAGTGC CAGAAATTCG AGGCGGCACT GTCCGAGTAC TGCGGCGGCC GTCCGGTCCG CGTATTCAAT TCGGGCACCT GCACGCTCGA GATCGGCCTG CGCATCGCGG GCGTCGGCCC CGGCGACGAG GTGATCACGA CGCCGGCGTC GTGGGTCTCG ACCAGCAACG TGATCATCGA AACGGGCGCG ACGCCCGTGT TCGCGGACAT CGATCCCGTC ACGCGCAACA TCGACCTCGA CAAGCTCGAG CAGGCGATCA CGCCGCGCAC GAAGGCGATC ATCCCCGTGT TCCTGTCCGG CCTGCCGGTC GACATGGACC GCCTGTACGC GATCGCCCGC GCGCACAAGC TGCGCGTGAT CGAGGATGCC GCGCAGGCGT TCGGCTCGAC GTGGCACGGC AAGCGCATCG GCGCGATCGG CGACCTCGTG TCGTTCAGCT TCCACGCGAA CAAGAACCTG ACGACGATCG AAGGCGGCGC GCTCGTGCTG AACAACGAGG ACGAAGCGGT GCTCGCGCAG AAGTACCGGC TGCAGGGCAT CACGCGCACC GGCTTCGACG GAATGGACTG CGACGTGCTC GGCGGCAAGT ACAACCTGAC CGACGTCGCC GCGCGCGTCG GCCTCGGCCA GCTGCCGCAC CTCGAGCGCT TCACCGCGCA GCGCCGCGCA CTCGCGCGCG CGTATTTCGC CGCGTTCGAC GGCGGCGCGG CCGCGAAGCT CGGCGTCGGC CTGCCCGTCG CCGAATTCGA GAACGGCAAC TGGCACATGT TCCTGGTCAC GCTGCCGCTC GAGCGGCTGA CGATCACGCG CGCCGAATTC ATGGCGCAGA TGAAGGAACG CGGCATCGGC ACGGGCATCC ACTACCCGGC GATCCATCTT TTCACGCTGT ACCGTGCGCG CGGGTTCAAG GAGGGCATGT TCCCCCACGC CGAACGGTAC GGCGCGTCGA CCGTCACGCT GCCGCTGTTT ACGCAGATGA CGGAAGGCGA CGTGCGTCGC GTCGTCGACG CCGTCAATCA GATTTGCGAA CAATACGGAA AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT