BuceA.17309.a

Putative ubiquinone biosynthesis-related protein

CENTER ID: BuceA.17309.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.17309.a.B2.GE35028 mature protein 33 395
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3456
RefSeq: YP_002232436.1
UniProt: B4EES1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTASSPATP DYDLAIVGAG PVGLALAGWL ARRSATQHAS IALIDAREPA ASANDPRAIA VSHGSRVLLD TLAWPADATP IEHIHVSQRG HFGRTLIDRD EHALAALGYV VRYGSIVQAL AGAVRGTRVD WLTSTTAHTP QQDADGVTLT LDGPQGERTL RARIVINAEG GLFHQQQADT GKHRRDYGQT ALVGTVTVSA PRPNVAWERF THEGPLALLP LGGPRQADYS LVWCCTPDEA ARRAALPDDA FLRELGSVFG ERMGEFVAIA GRASFPLGLN AAQTLVNGRI AIVGNAAQTL HPVAGQGLNL GLRDAHTLVD TLSAQGFEAT ALATFNARRA LDRRFTIGAT DTLARLFTID AGPLPLLRGA ALTALEFVPP LKKAIARQMM FGQRN
NT Sequence
atgacgaccg cttcctcccc ggccacgccg gactacgacc tcgccatcgt cggcgcgggc cccgtcgggc tcgcgctcgc cggctggctc gcccgccgca gcgcgacgca gcacgcgtcg atcgcgctga tcgatgcgcg cgaaccggcc gcgagcgcga acgacccgcg cgcgatcgcc gtgtcgcacg gcagccgcgt gctgctcgat acgctcgcat ggcccgccga cgccacgccg atcgaacata tccacgtgtc gcagcgcggc catttcggcc gcacgctgat cgaccgcgac gaacacgcgc tcgccgcgct cggctacgtg gtgcgctacg gctcgatcgt gcaggcactc gcgggcgccg tacgcggcac gcgcgtcgac tggctcacgt cgaccaccgc gcacacgccg cagcaggatg ccgacggcgt cacgctgacg ctcgacggcc cgcaaggcga acgcacgctg cgtgcgcgca tcgtcatcaa cgccgaaggc gggctgttcc accagcagca ggccgatacg ggcaagcatc gccgcgacta cggccagacc gcgctcgtcg gcacggtcac ggtatcggcg ccacgcccga acgtcgcgtg ggaacgcttc acgcacgaag gcccgctcgc gctgctgccg ctgggcgggc cgcgtcaggc cgactactcg ctcgtctggt gctgtacgcc cgacgaagcg gcgcgacggg ccgcgctgcc cgacgatgcg ttcctgcgcg agctcggcag cgtgttcggc gaacgcatgg gcgaattcgt cgcgatcgcc gggcgcgcgt cgttcccgct cggcctgaac gccgcgcaga cgctcgtcaa cggccgcatc gcgatcgtcg gcaatgccgc gcaaacgctg catccggtgg caggccaggg gctcaacctc ggcctgcgcg atgcacacac gctcgtcgac acactgtccg cacaaggctt cgaagcgacc gcgctcgcga ccttcaacgc gcgccgcgcg ctcgaccggc gcttcacgat cggcgcgacc gacacgctgg cgcgcctgtt tacgatcgat gcgggcccgc tgccgctgct gcgtggcgcg gcgctcaccg cgctcgaatt cgtgccgccg ctcaagaagg cgatcgcacg ccagatgatg ttcggccagc gcaactga
Details for BuceA.17309.a.B2.GE35028
HARVESTED ON: 3/19/2012
SEQUENCED ON: 3/22/2012
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR SATQHASIAL IDAREPAASA NDPRAIAVSH GSRVLLDTLA WPADATPIEH IHVSQRGHFG RTLIDRDEHA LAALGYVVRY GSIVQALAGA VRGTRVDWLT STTAHTPQQD ADGVTLTLDG PQGERTLRAR IVINAEGGLF HQQQADTGKH RRDYGQTALV GTVTVSAPRP NVAWERFTHE GPLALLPLGG PRQADYSLVW CCTPDEAARR AALPDDAFLR ELGSVFGERM GEFVAIAGRA SFPLGLNAAQ TLVNGRIAIV GNAAQTLHPV AGXGLNLGLR DAHTLVDXLS AQGXEAXXXX XXXXXRRXXX XXGXTXXLXX XSXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
annattttnn nnnntttaag aaggagatat accatggctc accaccacca ccaccatatg cgcagcgcga cgcagcacgc gtcgatcgcg ctgatcgatg cgcgcgaacc ggccgcgagc gcgaacgacc cgcgcgcgat cgccgtgtcg cacggcagcc gcgtgctgct cgatacgctc gcatggcccg ccgacgccac gccgatcgaa catatccacg tgtcgcagcg cggccatttc ggccgcacgc tgatcgaccg cgacgaacac gcgctcgccg cgctcggcta cgtggtgcgc tacggctcga tcgtgcaggc actcgcgggc gccgtacgcg gcacgcgcgt cgactggctc acgtcgacca ccgcgcacac gccgcagcag gatgccgacg gcgtcacgct gacgctcgac ggcccgcaag gcgaacgcac gctgcgtgcg cgcatcgtca tcaacgccga aggcgggctg ttccaccagc agcaggccga tacgggcaag catcgccgcg actacggcca gaccgcgctc gtcggcacgg tcacggtatc ggcgccacgc ccgaacgtcg cgtgggaacg cttcacgcac gaaggcccgc tcgcgctgct gccgctgggc gggccgcgtc aggccgacta ctcgctcgtc tggtgctgta cgcccgacga agcggcgcga cgggccgcgc tgcccgacga tgcgttcctg cgcgagctcg gcagcgtgtt cggcgaacgc atgggcgaat tcgtcgcgat cgccgggcgc gcgtcgttcc cgctcggcct gaacgccgcg cagacgctcg tcaacggccg catcgcgatc gtcggcaatg ccgcgcaaac gctgcatccg gtggcaggcc nnggnctcaa cctcggcctg cgcgatgcac acacgctcgt cgacncnctg tccgcacaag gctnngaagc gannngctng nganntnaan gngngcngcg tcgancggnn ttnnngatng gnnngaccga nnnnctggnn nnnngatcga tnnnggnnnc nnnnncnnnn nnnnnnnnnn nnnnnnncnn cgnnnnntnn nnnnncnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngnca
Expected Protein Sequence
MAHHHHHHMR SATQHASIAL IDAREPAASA NDPRAIAVSH GSRVLLDTLA WPADATPIEH IHVSQRGHFG RTLIDRDEHA LAALGYVVRY GSIVQALAGA VRGTRVDWLT STTAHTPQQD ADGVTLTLDG PQGERTLRAR IVINAEGGLF HQQQADTGKH RRDYGQTALV GTVTVSAPRP NVAWERFTHE GPLALLPLGG PRQADYSLVW CCTPDEAARR AALPDDAFLR ELGSVFGERM GEFVAIAGRA SFPLGLNAAQ TLVNGRIAIV GNAAQTLHPV AGQGLNLGLR DAHTLVDTLS AQGFEATALA TFNARRALDR RFTIGATDTL ARLFTIDAGP LPLLRGAALT ALEFVPPLKK AIARQMMFGQ RN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgca gcgcgacgca gcacgcgtcg atcgcgctga tcgatgcgcg cgaaccggcc gcgagcgcga acgacccgcg cgcgatcgcc gtgtcgcacg gcagccgcgt gctgctcgat acgctcgcat ggcccgccga cgccacgccg atcgaacata tccacgtgtc gcagcgcggc catttcggcc gcacgctgat cgaccgcgac gaacacgcgc tcgccgcgct cggctacgtg gtgcgctacg gctcgatcgt gcaggcactc gcgggcgccg tacgcggcac gcgcgtcgac tggctcacgt cgaccaccgc gcacacgccg cagcaggatg ccgacggcgt cacgctgacg ctcgacggcc cgcaaggcga acgcacgctg cgtgcgcgca tcgtcatcaa cgccgaaggc gggctgttcc accagcagca ggccgatacg ggcaagcatc gccgcgacta cggccagacc gcgctcgtcg gcacggtcac ggtatcggcg ccacgcccga acgtcgcgtg ggaacgcttc acgcacgaag gcccgctcgc gctgctgccg ctgggcgggc cgcgtcaggc cgactactcg ctcgtctggt gctgtacgcc cgacgaagcg gcgcgacggg ccgcgctgcc cgacgatgcg ttcctgcgcg agctcggcag cgtgttcggc gaacgcatgg gcgaattcgt cgcgatcgcc gggcgcgcgt cgttcccgct cggcctgaac gccgcgcaga cgctcgtcaa cggccgcatc gcgatcgtcg gcaatgccgc gcaaacgctg catccggtgg caggccaggg gctcaacctc ggcctgcgcg atgcacacac gctcgtcgac acactgtccg cacaaggctt cgaagcgacc gcgctcgcga ccttcaacgc gcgccgcgcg ctcgaccggc gcttcacgat cggcgcgacc gacacgctgg cgcgcctgtt tacgatcgat gcgggcccgc tgccgctgct gcgtggcgcg gcgctcaccg cgctcgaatt cgtgccgccg ctcaagaagg cgatcgcacg ccagatgatg ttcggccagc gcaactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat