SSGCID: Target Page

BuceA.17267.a

Putative peptidyl-prolyl cis-trans isomerase

CENTER ID:	BuceA.17267.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17267.a.A2.GE34648	Mature protein( BuceA.17267.a )		18	442

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17267.a.A2.PW35258	Mature protein( BuceA.17267.a )		18	442

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.939
RefSeq:	YP_002230049.1
UniProt:	B4EBE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSLAASAAL LAAAPAAAQA LGSQGAQLAD EVVAVVNNDV ITGRELDQRV GLIARRLQQQ NAPVPPADQL RGQVLNQMVL ERIQVQKAKD DGIRVDDATV QATLQRLAQA NGMTLDQYRA RLEAQGVPWS IFTNDARTEL TLSKLREREV DGKITVSDAE VANYIASQRG PNASQQQDLR FQHIFIKAPT NAPQADIEAA QKKAEALLQQ AKSGADFERL AKNNSEANDA KKGGDLGFKS PSALPADVVE AASKLRPGQV NPTLIRVPDG FEIVRLVDRR QSQGASAAAP KIVQTHVRHI LLRVGEGKSE GQARQQLVDI RNQVEAGGDF AKFARTYSQD GSASQGGDLG WISPGETVPE FERAMNNLQD GQISQPIRTE YGYHLIQVLN RREAEGSVQQ QMDIARQAIG QRKAEQAYAD WLRELRDSSY VQYKIGGVGP AN

NT Sequence

GTGTCCAGCC TTGCCGCGTC CGCCGCGCTG CTCGCCGCCG CGCCGGCCGC GGCGCAGGCG CTCGGTTCGC AAGGCGCGCA GCTCGCCGAC GAAGTCGTCG CGGTCGTCAA CAACGACGTG ATCACGGGCC GTGAACTCGA CCAGCGTGTC GGCTTGATCG CGCGCCGGCT GCAGCAGCAG AACGCGCCGG TGCCGCCGGC CGACCAGCTG CGCGGGCAGG TGCTGAACCA GATGGTGCTC GAACGTATCC AGGTGCAGAA GGCCAAGGAT GACGGGATCC GTGTCGACGA CGCGACCGTG CAGGCCACGC TGCAGCGTCT CGCGCAGGCG AACGGGATGA CGCTCGACCA GTACCGCGCG CGTCTCGAGG CGCAAGGCGT GCCCTGGAGC ATCTTCACGA ACGACGCGCG CACCGAGCTG ACGCTGTCGA AGCTGCGCGA GCGGGAGGTC GACGGCAAGA TCACGGTGTC GGACGCCGAG GTCGCGAACT ACATCGCGAG CCAGCGCGGG CCGAACGCGT CGCAGCAGCA GGACCTGCGC TTCCAGCACA TCTTCATCAA GGCGCCCACC AACGCGCCGC AGGCCGACAT CGAAGCCGCG CAGAAGAAGG CGGAAGCGCT GCTGCAGCAG GCGAAGTCGG GCGCCGATTT TGAGCGGCTC GCGAAGAACA ACTCCGAAGC GAACGACGCG AAGAAGGGCG GCGATCTCGG CTTCAAGTCG CCGAGCGCGC TGCCGGCCGA CGTCGTCGAG GCCGCGTCGA AGCTGCGCCC GGGCCAGGTC AACCCGACGC TGATCCGCGT GCCGGACGGC TTCGAGATCG TGCGCCTCGT CGATCGCCGC CAGAGCCAGG GCGCGAGCGC CGCGGCGCCG AAGATCGTCC AGACGCACGT GCGCCACATC CTGCTGCGCG TCGGCGAAGG CAAGTCGGAA GGCCAGGCGC GCCAGCAACT CGTTGACATC CGCAACCAGG TCGAGGCCGG CGGCGATTTC GCGAAGTTCG CGCGCACCTA CTCGCAGGAC GGGTCGGCGT CGCAGGGCGG CGATCTCGGC TGGATCAGCC CGGGCGAGAC CGTGCCGGAA TTCGAGCGTG CGATGAACAA CCTGCAGGAC GGCCAGATCA GCCAGCCGAT TCGTACCGAG TACGGCTACC ACCTGATCCA GGTGCTGAAC CGCCGCGAAG CGGAAGGGTC GGTGCAGCAG CAGATGGACA TCGCCCGCCA GGCGATCGGC CAGCGCAAGG CCGAACAGGC CTATGCGGAC TGGCTGCGCG AGCTGCGCGA TTCGTCGTAC GTGCAGTACA AGATCGGCGG CGTCGGCCCG GCGAAC

Details for BuceA.17267.a.A2.GE34648

HARVESTED ON:	1/31/2012
SEQUENCED ON:	2/3/2012
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAQALGSQG AQLADEVVAV VNNDVITGRE LDQRVGLIAR RLQQQNAPVP PADQLRGQVL NQMVLERIQV QKAKDDGIRV DDATVQATLQ RLAQANGMTL DQYRARLEAQ GVPWSIFTND ARTELTLSKL REREVDGKIT VSDAEVANYI ASQRGPNASQ QQDLRFQHIF IKAPTNAPQA DIEAAQKKAE ALLQQAKSGA DFERLAKNNS EANDAKKGGD LGFKSPSALP ADVVEAASKL RPGQVNPTLI RVPDGFEIVR LVDRRQSQGA SAAAPKIVQT HVRHILLRVG EGKSEGQARQ QLVDIRNQVE AGGDFAKFAR TYSQDGSASQ GGDLGWISPG ETVPEFERAM NNLQDGQISQ PIRTEYGYHL IQVLNRREAE GSVQQQMDIA RQAIGQRKAE QAYADWLREL RDSSYVQYKI GGVGP

Validated NT Sequence

taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gcgcaggcgc tcggttcgca aggcgcgcag ctcgccgacg aagtcgtcgc ggtcgtcaac aacgacgtga tcacgggccg tgaactcgac cagcgtgtcg gcttgatcgc gcgccggctg cagcagcaga acgcgccggt gccgccggcc gaccagctgc gcgggcaggt gctgaaccag atggtgctcg aacgtatcca ggtgcagaag gccaaggatg acgggatccg tgtcgacgac gcgaccgtgc aggccacgct gcagcgtctc gcgcaggcga acgggatgac gctcgaccag taccgcgcgc gtctcgaggc gcaaggcgtg ccctggagca tcttcacgaa cgacgcgcgc accgagctga cgctgtcgaa gctgcgcgag cgggaggtcg acggcaagat cacggtgtcg gacgccgagg tcgcgaacta catcgcgagc cagcgcgggc cgaacgcgtc gcagcagcag gacctgcgct tccagcacat cttcatcaag gcgcccacca acgcgccgca ggccgacatc gaagccgcgc agaagaaggc ggaagcgctg ctgcagcagg cgaagtcggg cgccgatttt gagcggctcg cgaagaacaa ctccgaagcg aacgacgcga agaagggcgg cgatctcggc ttcaagtcgc cgagcgcgct gccggccgac gtcgtcgagg ccgcgtcgaa gctgcgcccg ggccaggtca acccgacgct gatccgcgtg ccggacggct tcgagatcgt gcgcctcgtc gatcgccgcc agagccaggg cgcgagcgcc gcggcgccga agatcgtcca gacgcacgtg cgccacatcc tgctgcgcgt cggcgaaggc aagtcggaag gccaggcgcg ccagcaactc gttgacatcc gcaaccaggt cgaggccggc ggcgatttcg cgaagttcgc gcgcacctac tcgcaggacg ggtcggcgtc gcagggcggc gatctcggct ggatcagccc gggcgagacc gtgccggaat tcgagcgtgc gatgaacaac ctgcaggacg gccagatcag ccagccgatt cgtaccgagt acggctacca cctgatccag gtgctgaacc gccgcgaagc ggaagggtcg gtgcagcagc agatggacat cgcccgccag gcgatcggcc agcgcaaggc cgaacaggcc tatgcggact ggctgcgcga gctgcgcgat tcgtcgtacg tgcagtacaa gatcggcggc gtcggcccgg c

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgcaggcg ctcggttcgc aaggcgcgca gctcgccgac gaagtcgtcg cggtcgtcaa caacgacgtg atcacgggcc gtgaactcga ccagcgtgtc ggcttgatcg cgcgccggct gcagcagcag aacgcgccgg tgccgccggc cgaccagctg cgcgggcagg tgctgaacca gatggtgctc gaacgtatcc aggtgcagaa ggccaaggat gacgggatcc gtgtcgacga cgcgaccgtg caggccacgc tgcagcgtct cgcgcaggcg aacgggatga cgctcgacca gtaccgcgcg cgtctcgagg cgcaaggcgt gccctggagc atcttcacga acgacgcgcg caccgagctg acgctgtcga agctgcgcga gcgggaggtc gacggcaaga tcacggtgtc ggacgccgag gtcgcgaact acatcgcgag ccagcgcggg ccgaacgcgt cgcagcagca ggacctgcgc ttccagcaca tcttcatcaa ggcgcccacc aacgcgccgc aggccgacat cgaagccgcg cagaagaagg cggaagcgct gctgcagcag gcgaagtcgg gcgccgattt tgagcggctc gcgaagaaca actccgaagc gaacgacgcg aagaagggcg gcgatctcgg cttcaagtcg ccgagcgcgc tgccggccga cgtcgtcgag gccgcgtcga agctgcgccc gggccaggtc aacccgacgc tgatccgcgt gccggacggc ttcgagatcg tgcgcctcgt cgatcgccgc cagagccagg gcgcgagcgc cgcggcgccg aagatcgtcc agacgcacgt gcgccacatc ctgctgcgcg tcggcgaagg caagtcggaa ggccaggcgc gccagcaact cgttgacatc cgcaaccagg tcgaggccgg cggcgatttc gcgaagttcg cgcgcaccta ctcgcaggac gggtcggcgt cgcagggcgg cgatctcggc tggatcagcc cgggcgagac cgtgccggaa ttcgagcgtg cgatgaacaa cctgcaggac ggccagatca gccagccgat tcgtaccgag tacggctacc acctgatcca ggtgctgaac cgccgcgaag cggaagggtc ggtgcagcag cagatggaca tcgcccgcca ggcgatcggc cagcgcaagg ccgaacaggc ctatgcggac tggctgcgcg agctgcgcga ttcgtcgtac gtgcagtaca agatcggcgg cgtcggcccg gcgaactaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BuceA.17267.a.A2.PW35258

PURIFICATION DATe:	3/16/2012
CONCENTRATION:	31.79mg/ml
OBSERVED MW:	49kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	14
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCAGGCG CTCGGTTCGC AAGGCGCGCA GCTCGCCGAC GAAGTCGTCG CGGTCGTCAA CAACGACGTG ATCACGGGCC GTGAACTCGA CCAGCGTGTC GGCTTGATCG CGCGCCGGCT GCAGCAGCAG AACGCGCCGG TGCCGCCGGC CGACCAGCTG CGCGGGCAGG TGCTGAACCA GATGGTGCTC GAACGTATCC AGGTGCAGAA GGCCAAGGAT GACGGGATCC GTGTCGACGA CGCGACCGTG CAGGCCACGC TGCAGCGTCT CGCGCAGGCG AACGGGATGA CGCTCGACCA GTACCGCGCG CGTCTCGAGG CGCAAGGCGT GCCCTGGAGC ATCTTCACGA ACGACGCGCG CACCGAGCTG ACGCTGTCGA AGCTGCGCGA GCGGGAGGTC GACGGCAAGA TCACGGTGTC GGACGCCGAG GTCGCGAACT ACATCGCGAG CCAGCGCGGG CCGAACGCGT CGCAGCAGCA GGACCTGCGC TTCCAGCACA TCTTCATCAA GGCGCCCACC AACGCGCCGC AGGCCGACAT CGAAGCCGCG CAGAAGAAGG CGGAAGCGCT GCTGCAGCAG GCGAAGTCGG GCGCCGATTT TGAGCGGCTC GCGAAGAACA ACTCCGAAGC GAACGACGCG AAGAAGGGCG GCGATCTCGG CTTCAAGTCG CCGAGCGCGC TGCCGGCCGA CGTCGTCGAG GCCGCGTCGA AGCTGCGCCC GGGCCAGGTC AACCCGACGC TGATCCGCGT GCCGGACGGC TTCGAGATCG TGCGCCTCGT CGATCGCCGC CAGAGCCAGG GCGCGAGCGC CGCGGCGCCG AAGATCGTCC AGACGCACGT GCGCCACATC CTGCTGCGCG TCGGCGAAGG CAAGTCGGAA GGCCAGGCGC GCCAGCAACT CGTTGACATC CGCAACCAGG TCGAGGCCGG CGGCGATTTC GCGAAGTTCG CGCGCACCTA CTCGCAGGAC GGGTCGGCGT CGCAGGGCGG CGATCTCGGC TGGATCAGCC CGGGCGAGAC CGTGCCGGAA TTCGAGCGTG CGATGAACAA CCTGCAGGAC GGCCAGATCA GCCAGCCGAT TCGTACCGAG TACGGCTACC ACCTGATCCA GGTGCTGAAC CGCCGCGAAG CGGAAGGGTC GGTGCAGCAG CAGATGGACA TCGCCCGCCA GGCGATCGGC CAGCGCAAGG CCGAACAGGC CTATGCGGAC TGGCTGCGCG AGCTGCGCGA TTCGTCGTAC GTGCAGTACA AGATCGGCGG CGTCGGCCCG GCGAACTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA