SSGCID: Target Page

BuceA.17266.b

Putative peptidylprolyl isomerase

CENTER ID:	BuceA.17266.b
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17266.b.A2.GE31894	Mature protein( BuceA.17266.b )		26	280

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.3901
RefSeq:	YP_002232848.1
UniProt:	B4EHP7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKHHISIVS TLIALACACG APVARAAGTK TDTPLPPGVE AVVNDTPIAR SDVDSMIEAT GQTVTPELRA QAKRELIAQH LVEQAAEKAN YGSRPEVNRV VMRARTVAAT DLYLRDNVHP QAVTDAQVKA RYDDIVANAA QFEYRADVIA VADPAEVNAL VAELKQGAAF DAVAKKYNTT PNGGVAQWVE LRTPVAEGKT GGLPLPLAQA ITSLQPGAVA GPIRVGNAFA IVKLDEKRAT VVPTFEAAKN VLRQQLEGQA QQRAMTALVD KLAGQATIQQ

NT Sequence

ATGAAGAAGC ATCACATTTC CATTGTTTCC ACACTGATCG CACTCGCGTG CGCCTGCGGC GCCCCCGTCG CGCGCGCCGC AGGCACGAAA ACCGACACGC CGCTGCCGCC GGGCGTCGAA GCGGTCGTCA ACGACACACC CATCGCGCGC AGCGATGTGG ACAGCATGAT CGAAGCGACC GGACAGACCG TCACGCCCGA ATTGCGCGCA CAAGCCAAGC GTGAGCTGAT CGCCCAGCAT CTGGTCGAGC AGGCGGCGGA AAAAGCCAAC TACGGCAGCC GTCCCGAAGT GAACCGCGTC GTGATGCGTG CGCGCACGGT CGCGGCGACG GATCTCTACC TGCGTGACAA CGTGCACCCG CAAGCGGTGA CCGATGCGCA GGTGAAGGCG CGTTACGACG ACATCGTCGC GAATGCCGCG CAGTTCGAAT ATCGCGCCGA CGTGATCGCC GTAGCCGATC CTGCCGAAGT GAACGCGCTG GTCGCGGAGC TGAAGCAGGG CGCTGCGTTC GACGCGGTGG CGAAGAAGTA CAACACGACG CCGAACGGCG GCGTTGCGCA GTGGGTCGAA TTGCGTACGC CGGTGGCCGA GGGGAAGACG GGCGGTTTGC CGTTACCGCT CGCGCAGGCG ATTACGTCGC TGCAGCCGGG TGCCGTCGCA GGCCCGATCC GGGTCGGTAA CGCGTTCGCG ATCGTGAAAC TCGACGAGAA GCGCGCGACG GTCGTGCCGA CCTTCGAAGC GGCGAAGAAC GTGCTGCGTC AGCAGCTGGA AGGGCAGGCG CAGCAGCGTG CGATGACGGC GCTGGTGGAC AAGCTGGCCG GGCAGGCGAC GATTCAGCAG

Details for BuceA.17266.b.A2.GE31894

HARVESTED ON:	3/22/2011
SEQUENCED ON:	3/25/2011
EXPECTED MW:	29kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAAGTKTDT PLPPGVEAVV NDTPIARSDV DSMIEATGQT VTPELRAQAK RELIAQHLVE QAAEKANYGS RPEVNRVVMR ARTVAATDLY LRDNVHPQAV TDAQVKARYD DIVANAAQFE YRADVIAVAD PAEVNALVAE LKQGAAFDAV AKKYNTTPNG GVAQWVELRT PVAEGKTGGL PLPLAQAITS LQPGAVAGPI RVGNAFAIVK LDEKRATVVP TFEAAKNVLR QQLEGQAQQR AMTALVDKLA GQAT

Validated NT Sequence

aatcgtcgcc tgcccggcca gcttgtccac cagcgccgtc atcgcacgct gctgcgcctg cccttccagc tgctgacgca gcacgttctt cgccgcttcg aaggtcggca cgaccgtcgc gcgcttctcg tcgagtttca cgatcgcgaa cgcgttaccg acccggatcg ggcctgcgac ggcacccggc tgcagcgacg taatcgcctg cgcgagcggt aacggcaaac cgcccgtctt cccctcggcc accggcgtac gcaattcgac ccactgcgca acgccgccgt tcggcgtcgt gttgtacttc ttcgccaccg cgtcgaacgc agcgccctgc ttcagctccg cgaccagcgc gttcacttcg gcaggatcgg ctacggcgat cacgtcggcg cgatattcga actgcgcggc attcgcgacg atgtcgtcgt aacgcgcctt cacctgcgca tcggtcaccg cttgcgggtg cacgttgtca cgcaggtaga gatccgtcgc cgcgaccgtg cgcgcacgca tcacgacgcg gttcacttcg ggacggctgc cgtagttggc tttttccgcc gcctgctcga ccagatgctg ggcgatcagc tcacgcttgg cttgtgcgcg caattcgggc gtgacggtct gtccggtcgc ttcgatcatg ctgtccacat cgctgcgcgc gatgggtgtg tcgttgacga ccgcttcgac gcccggcggc agcggcgtgt cggttttcgt gcctgcggcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccatggta

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgcaggc acgaaaaccg acacgccgct gccgccgggc gtcgaagcgg tcgtcaacga cacacccatc gcgcgcagcg atgtggacag catgatcgaa gcgaccggac agaccgtcac gcccgaattg cgcgcacaag ccaagcgtga gctgatcgcc cagcatctgg tcgagcaggc ggcggaaaaa gccaactacg gcagccgtcc cgaagtgaac cgcgtcgtga tgcgtgcgcg cacggtcgcg gcgacggatc tctacctgcg tgacaacgtg cacccgcaag cggtgaccga tgcgcaggtg aaggcgcgtt acgacgacat cgtcgcgaat gccgcgcagt tcgaatatcg cgccgacgtg atcgccgtag ccgatcctgc cgaagtgaac gcgctggtcg cggagctgaa gcagggcgct gcgttcgacg cggtggcgaa gaagtacaac acgacgccga acggcggcgt tgcgcagtgg gtcgaattgc gtacgccggt ggccgagggg aagacgggcg gtttgccgtt accgctcgcg caggcgatta cgtcgctgca gccgggtgcc gtcgcaggcc cgatccgggt cggtaacgcg ttcgcgatcg tgaaactcga cgagaagcgc gcgacggtcg tgccgacctt cgaagcggcg aagaacgtgc tgcgtcagca gctggaaggg caggcgcagc agcgtgcgat gacggcgctg gtggacaagc tggccgggca ggcgacgatt cagcagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa