SSGCID: Target Page

BuceA.17229.a

4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1 (IspH/LytB) (IspG/H)

CENTER ID:	BuceA.17229.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17229.a.A1.GE31888	Full length( BuceA.17229.a )		1	326

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.17229.a.A1.PS01125	Full length( BuceA.17229.a )		1	326

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2806
RefSeq:	YP_002231814.1
UniProt:	B4E8Y4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTTDTLSGQ TVAADAEILL AQPRGFCAGV DRAIEIVERA IAMHGAPIYV RHEIVHNKYV VEDLKKKGAI FVEELEEVPA GNTVIFSAHG VSKAVRDEAD VRGLRIYDAT CPLVTKVHVE VAKMRQDGVD IVMIGHKGHP EVEGTMGQVE RGMHLVESVE DVQKLELADP ERIALVTQTT LSVDDAAEII GALKAKYPKI REPKKQDICY ATQNRQDAVK FMAPQCDVVI VVGSPNSSNS SRLREVAEKR GVAAYMVDAP DQIDPAWVAG KRRIGVTAGA SAPEVLAQAV IARLRELGVR NVRALDGIEE NVSFPLPRGL NLPPAA

NT Sequence

ATGAGTACCA CCGATACGCT GTCCGGGCAG ACCGTCGCCG CCGACGCCGA AATCCTGCTG GCCCAGCCGC GCGGTTTCTG CGCGGGTGTC GATCGCGCGA TCGAGATCGT CGAGCGCGCC ATCGCGATGC ACGGTGCGCC GATCTACGTG CGTCACGAAA TCGTCCACAA CAAGTACGTG GTCGAGGATC TGAAAAAGAA GGGCGCGATC TTCGTCGAGG AACTCGAGGA AGTGCCGGCC GGCAACACCG TGATCTTCAG TGCGCACGGC GTGTCGAAGG CCGTGCGCGA CGAGGCCGAC GTGCGCGGGC TGCGCATTTA CGACGCAACC TGCCCGCTCG TCACGAAGGT GCACGTCGAA GTGGCGAAGA TGCGCCAGGA CGGCGTCGAC ATCGTGATGA TCGGCCACAA GGGCCATCCC GAAGTCGAAG GCACGATGGG CCAGGTCGAG CGCGGCATGC ACCTCGTCGA AAGTGTCGAG GACGTGCAGA AGCTCGAGCT CGCCGATCCC GAGCGCATCG CGCTCGTCAC GCAGACGACG CTGTCCGTTG ACGACGCGGC CGAGATCATC GGCGCGCTGA AGGCAAAGTA TCCGAAGATC CGCGAGCCGA AGAAGCAGGA CATCTGCTAC GCGACGCAGA ACCGCCAGGA CGCGGTGAAG TTCATGGCGC CGCAATGCGA TGTCGTGATC GTGGTCGGTA GTCCGAACAG CTCGAACTCG AGCCGGCTGC GCGAAGTGGC CGAGAAGCGC GGCGTGGCCG CGTACATGGT CGATGCACCG GACCAGATCG ATCCGGCGTG GGTCGCCGGC AAGCGCCGCA TCGGCGTGAC GGCCGGTGCG TCGGCGCCCG AGGTGCTCGC CCAGGCCGTG ATCGCGCGGC TGCGCGAGCT CGGCGTGCGC AACGTCCGCG CACTCGACGG CATCGAGGAA AACGTGTCGT TTCCGCTGCC GCGCGGGTTG AACCTGCCGC CCGCCGCC

Details for BuceA.17229.a.A1.GE31888

HARVESTED ON:	3/22/2011
SEQUENCED ON:	3/25/2011
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ttattaggcg gcgggcggca ggttcaaccc gcgcggcagc ggaaacgaca cgttttcctc gatgccgtcn agtgcgcgga cgttgcgcac gccgagctcg cgcagccgcg cgatcacggc ctgggcgagc acctcgggcg ccgacgcacc ggccgtcacg ccgatgcggc gcttgccggc gacccacgcc ggatcgatct ggtccggtgc atcgaccatg tacgcggcca cgccgcgctt ctcggccact tcgcgcagcc ggctcgagtt cgagctgttc ggactaccga ccacgatcac gacatcgcat tgcggcgcca tgaacttcac cgcgtcctgg cggttctgcg tcgcgtagca gatgtcctgc ttcttcggct cgcggatctt cggatacttt gccttcagcg cgccgatgat ctcggccgcg tcgtcaacgg acagcgtcgt ctgcgtgacg agcgcgatgc gctcgggatc ggcgagctcg agcttctgca cgtcctcgac actttcgacg aggtgcatgc cgcgctcgac ctggcccatc gtgccttcga cttcgggatg gcccttgtgg ccgatcatca cgatgtcgac gccgtcctgg cgcatcttcg ccacttcgac gtgcaccttc gtgacgagcg ggcaggttgc gtcgtaaatg cgcagcccgc gcacgtcggc ctcgtcgcgc acggccttcg acacgccgtg cgcactgaag atcacggtgt tgccggccgg cacttcctcg agttcctcga cgaagatcgc gcccttcttt ttcagatcct cgaccacgta cttgttgtgg acgatttcgt gacgcacgta gatcggcgca ccgtgcatcg cgatggcgcg ctcgacgatc tcgatcgcgc gatcgacacc cgcgcagaaa ccgcgcggct gggccagcag gatttcggcg tcggcggcga cggtctgccc ggacagcgta tcggtggtac tcatcgaacc aggaccctgg gtctgagctt ccagggtacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTTDTLSG QTVAADAEIL LAQPRGFCAG VDRAIEIVER AIAMHGAPIY VRHEIVHNKY VVEDLKKKGA IFVEELEEVP AGNTVIFSAH GVSKAVRDEA DVRGLRIYDA TCPLVTKVHV EVAKMRQDGV DIVMIGHKGH PEVEGTMGQV ERGMHLVESV EDVQKLELAD PERIALVTQT TLSVDDAAEI IGALKAKYPK IREPKKQDIC YATQNRQDAV KFMAPQCDVV IVVGSPNSSN SSRLREVAEK RGVAAYMVDA PDQIDPAWVA GKRRIGVTAG ASAPEVLAQA VIARLRELGV RNVRALDGIE ENVSFPLPRG LNLPPAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtaccacc gatacgctgt ccgggcagac cgtcgccgcc gacgccgaaa tcctgctggc ccagccgcgc ggtttctgcg cgggtgtcga tcgcgcgatc gagatcgtcg agcgcgccat cgcgatgcac ggtgcgccga tctacgtgcg tcacgaaatc gtccacaaca agtacgtggt cgaggatctg aaaaagaagg gcgcgatctt cgtcgaggaa ctcgaggaag tgccggccgg caacaccgtg atcttcagtg cgcacggcgt gtcgaaggcc gtgcgcgacg aggccgacgt gcgcgggctg cgcatttacg acgcaacctg cccgctcgtc acgaaggtgc acgtcgaagt ggcgaagatg cgccaggacg gcgtcgacat cgtgatgatc ggccacaagg gccatcccga agtcgaaggc acgatgggcc aggtcgagcg cggcatgcac ctcgtcgaaa gtgtcgagga cgtgcagaag ctcgagctcg ccgatcccga gcgcatcgcg ctcgtcacgc agacgacgct gtccgttgac gacgcggccg agatcatcgg cgcgctgaag gcaaagtatc cgaagatccg cgagccgaag aagcaggaca tctgctacgc gacgcagaac cgccaggacg cggtgaagtt catggcgccg caatgcgatg tcgtgatcgt ggtcggtagt ccgaacagct cgaactcgag ccggctgcgc gaagtggccg agaagcgcgg cgtggccgcg tacatggtcg atgcaccgga ccagatcgat ccggcgtggg tcgccggcaa gcgccgcatc ggcgtgacgg ccggtgcgtc ggcgcccgag gtgctcgccc aggccgtgat cgcgcggctg cgcgagctcg gcgtgcgcaa cgtccgcgca ctcgacggca tcgaggaaaa cgtgtcgttt ccgctgccgc gcgggttgaa cctgccgccc gccgcctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BuceA.17229.a.A1.PS01125

PURIFICATION DATe:	6/21/2011
CONCENTRATION:	27.31mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTACCACC GATACGCTGT CCGGGCAGAC CGTCGCCGCC GACGCCGAAA TCCTGCTGGC CCAGCCGCGC GGTTTCTGCG CGGGTGTCGA TCGCGCGATC GAGATCGTCG AGCGCGCCAT CGCGATGCAC GGTGCGCCGA TCTACGTGCG TCACGAAATC GTCCACAACA AGTACGTGGT CGAGGATCTG AAAAAGAAGG GCGCGATCTT CGTCGAGGAA CTCGAGGAAG TGCCGGCCGG CAACACCGTG ATCTTCAGTG CGCACGGCGT GTCGAAGGCC GTGCGCGACG AGGCCGACGT GCGCGGGCTG CGCATTTACG ACGCAACCTG CCCGCTCGTC ACGAAGGTGC ACGTCGAAGT GGCGAAGATG CGCCAGGACG GCGTCGACAT CGTGATGATC GGCCACAAGG GCCATCCCGA AGTCGAAGGC ACGATGGGCC AGGTCGAGCG CGGCATGCAC CTCGTCGAAA GTGTCGAGGA CGTGCAGAAG CTCGAGCTCG CCGATCCCGA GCGCATCGCG CTCGTCACGC AGACGACGCT GTCCGTTGAC GACGCGGCCG AGATCATCGG CGCGCTGAAG GCAAAGTATC CGAAGATCCG CGAGCCGAAG AAGCAGGACA TCTGCTACGC GACGCAGAAC CGCCAGGACG CGGTGAAGTT CATGGCGCCG CAATGCGATG TCGTGATCGT GGTCGGTAGT CCGAACAGCT CGAACTCGAG CCGGCTGCGC GAAGTGGCCG AGAAGCGCGG CGTGGCCGCG TACATGGTCG ATGCACCGGA CCAGATCGAT CCGGCGTGGG TCGCCGGCAA GCGCCGCATC GGCGTGACGG CCGGTGCGTC GGCGCCCGAG GTGCTCGCCC AGGCCGTGAT CGCGCGGCTG CGCGAGCTCG GCGTGCGCAA CGTCCGCGCA CTCGACGGCA TCGAGGAAAA CGTGTCGTTT CCGCTGCCGC GCGGGTTGAA CCTGCCGCCC GCCGCCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA