BuceA.17071.a

Putative exported protein

CENTER ID: BuceA.17071.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.17071.a.A2.GE34660 Mature protein( BuceA.17071.a ) 40 724
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.17071.a.A2.PS01483 Mature protein( BuceA.17071.a ) 40 724

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1548
RefSeq: YP_002230620.1
UniProt: B4E7J4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIFDWFSGL RRTVAPGSPT TSRVFGCGAL ALAVLCTGCA ESAAQRDSNT SIASTKIERT KAERLAREQQ IARTVPSLAS ISLTQPRPFT LRDSGQNGAM TFLRDVDFRI VNNLGFFIHQ LSATLVPTQA GAPIVFDDPT SFEIDVHEGT VTLDNAKLTA LFNTYIFGYR NAPLRKLAVS AGDGVIHLQG EMQRDGWVPF SLTGTLAIRD GSQLVFHPTG VRVSGINAQP VMRAANVKMA DLLKVQTPIA QLAGDDLVMA VDKLMPPPRL KLTITAVRVT PAGLDLKLDD GTHAGFAMPA NAPQQAMYIR GGDVKFMRSM PMNADILIGP VDSSKREQTF VFDLYHYRDQ VSAGYFNFDE SGAMAIRMPS YTGPASGALL GNAAARLNDS FLAAQQTALR DVRQHWEAYA LAADAMRPGM QKVAMRRTST TPFNERHVSN RNPTIHLRNV DFNLSGDIGF HVEDLDVELV SRRPGEPVDL DDPNQYDIRI LGGTVVESWK AMSALFNNYL LDYSPRSLND LQLSADGQNL RVQGGIKLWN HVPGVWLPTD MKGSLTVLDD RHLAFKPSQV SVLGIPQATL LRSLGIELAS LTPLRRRGAE LRGDTLVLDQ YTVFPPPVLN GKLGQATVER DGLRLTFRRA AGAAAPKRPQ IDAPSYMWME GGDMKMFNVL ELNVRALIRN SAEAGPMRFD LYGYRSQVAQ GSVRMLPDGT LVVDIGQKNP LASR
NT Sequence
TTGGCAATTT TCGACTGGTT TTCCGGCCTG CGACGCACGG TCGCCCCGGG ATCGCCCACG ACGTCCCGAG TGTTCGGCTG CGGCGCGCTG GCGCTTGCAG TCCTCTGCAC CGGCTGCGCG GAATCGGCCG CGCAGCGCGA CAGCAACACG TCCATCGCAT CGACGAAGAT CGAACGCACC AAGGCCGAGC GACTCGCGCG CGAACAGCAG ATCGCGCGCA CCGTGCCGTC GCTCGCATCG ATCAGCCTCA CGCAGCCGCG CCCGTTCACG CTGCGCGACT CGGGCCAGAA CGGCGCGATG ACGTTCCTGC GCGACGTCGA TTTCCGCATC GTCAACAACC TCGGCTTCTT CATCCACCAG TTGTCGGCCA CGCTCGTGCC GACGCAGGCC GGCGCGCCGA TCGTCTTCGA CGATCCGACG AGCTTCGAGA TCGACGTACA TGAAGGCACG GTGACGCTCG ACAACGCGAA GCTGACCGCC CTCTTCAACA CCTACATCTT CGGCTATCGC AACGCGCCGC TGCGCAAGCT CGCAGTGTCG GCCGGCGACG GCGTGATCCA TCTGCAAGGC GAGATGCAGC GCGACGGCTG GGTACCGTTC TCGCTGACCG GCACGCTCGC GATCCGCGAC GGCAGCCAGC TCGTGTTCCA CCCGACCGGC GTACGCGTGT CGGGTATCAA CGCGCAGCCG GTGATGCGCG CGGCCAACGT GAAGATGGCC GACCTGCTGA AGGTGCAGAC CCCGATCGCG CAACTTGCCG GCGACGATCT CGTGATGGCG GTCGACAAGC TGATGCCGCC ACCGCGCCTG AAGCTCACGA TCACCGCCGT GCGCGTGACG CCGGCAGGCC TCGACCTGAA GCTCGACGAC GGCACGCATG CCGGCTTCGC GATGCCGGCG AATGCCCCGC AGCAGGCGAT GTACATCCGC GGCGGCGACG TGAAGTTCAT GCGCTCGATG CCGATGAACG CCGACATCCT GATCGGTCCG GTCGATTCGT CGAAGCGCGA GCAGACGTTC GTGTTCGACC TGTATCACTA TCGCGACCAG GTGTCGGCCG GTTACTTCAA CTTCGACGAA AGCGGCGCGA TGGCGATCCG GATGCCTTCG TATACGGGCC CGGCGAGCGG CGCGCTGCTC GGCAACGCGG CGGCGCGCCT GAACGACAGC TTCCTCGCCG CGCAGCAGAC TGCGCTGCGC GACGTGCGCC AGCACTGGGA AGCGTACGCG CTCGCGGCGG ATGCGATGCG GCCCGGCATG CAGAAGGTCG CGATGCGGCG CACGTCGACG ACGCCGTTCA ACGAACGGCA CGTGTCGAAC CGCAACCCGA CGATCCATCT GCGCAACGTC GACTTCAACC TGTCCGGCGA TATCGGCTTT CATGTCGAGG ATCTCGACGT GGAACTCGTG TCGAGGCGGC CCGGCGAACC GGTCGATCTC GACGATCCGA ACCAGTACGA CATCCGCATT CTCGGCGGCA CGGTCGTCGA ATCGTGGAAG GCGATGTCGG CGCTCTTCAA CAACTACCTG CTCGACTATT CGCCGCGTTC GCTGAACGAC CTGCAACTGA GCGCGGACGG GCAGAACCTG CGCGTCCAGG GCGGCATCAA GCTGTGGAAT CACGTGCCGG GGGTCTGGCT GCCGACCGAC ATGAAGGGCT CGCTGACGGT GCTCGACGAC CGGCACCTCG CCTTCAAGCC GTCGCAGGTG TCGGTGCTCG GCATCCCGCA GGCGACGCTG CTGCGCTCGC TCGGCATCGA GCTCGCGTCG CTGACGCCGT TGCGCCGACG CGGCGCGGAA CTGCGCGGCG ACACGCTCGT GCTCGACCAG TACACCGTGT TCCCGCCGCC CGTGCTGAAC GGCAAGCTCG GCCAGGCAAC GGTCGAACGC GACGGCTTGC GTCTCACGTT CCGGCGCGCA GCGGGTGCAG CCGCGCCGAA GCGGCCGCAG ATCGATGCGC CGAGCTACAT GTGGATGGAA GGCGGCGACA TGAAGATGTT CAACGTGCTC GAGCTGAACG TGCGCGCGTT GATCCGGAAT TCGGCCGAGG CGGGCCCGAT GCGGTTCGAC CTGTACGGAT ACCGCAGCCA GGTCGCGCAG GGCTCGGTGC GGATGCTGCC GGACGGCACG CTGGTCGTCG ACATCGGGCA GAAGAATCCG CTCGCGTCGC GC
Details for BuceA.17071.a.A2.GE34660
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 77kDa
OBSERVED MW: 77kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 44
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAESAAQRD SNTSIASTKI ERTKAERLAR EQQIARTVPS LASISLTQPR PFTLRDSGQN GAMTFLRDVD FRIVNNLGFF IHQLSATLVP TQAGAPIVFD DPTSFEIDVH EGTVTLDNAK LTALFNTYIF GYRNAPLRKL AVSAGDGVIH LQGEMQRDGW VPFSLTGTLA IRDGSQLVFH PTGVRVSGIN AQPVMRAANV KMADLLKVQT PIAQLAGDDL VMAVDKLMPP PRLKLTITAV RVTPAGLDLK LDDGTHAGFX MPANAPQXXM YIRGGDVKFM RSMPMNAXXL IGPVD
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcggaa tcggccgcgc agcgcgacag caacacgtcc atcgcatcga cgaagatcga acgcaccaag gccgagcgac tcgcgcgcga acagcagatc gcgcgcaccg tgccgtcgct cgcatcgatc agcctcacgc agccgcgccc gttcacgctg cgcgactcgg gccagaacgg cgcgatgacg ttcctgcgcg acgtcgattt ccgcatcgtc aacaacctcg gcttcttcat ccaccagttg tcggccacgc tcgtgccgac gcaggccggc gcgccgatcg tcttcgacga tccgacgagc ttcgagatcg acgtacatga aggcacggtg acgctcgaca acgcgaagct gaccgccctc ttcaacacct acatcttcgg ctatcgcaac gcgccgctgc gcaagctcgc agtgtcggcc ggcgacggcg tgatccatct gcaaggcgag atgcagcgcg acggctgggt accgttctcg ctgaccggca cgctcgcgat ccgcgacggc agccagctcg tgttccaccc gaccggcgta cgcgtgtcgg gtatcaacgc gcagccggtg atgcgcgcgg ccaacgtgaa gatggccgac ctgctgaagg tgcagacccc gatcgcgcaa cttgccggcg acgatctcgt gatggcggtc gacaagctga tgccgccacc gcgcctgaag ctcacgatca ccgccgtgcg cgtgacgccn gcaggnctcg acctgaagct cgacgacggc acgcatgccg gcttcncgat gccggcgaat gccccgcagc nggngatgta catccgcggc ggcgacgtga agttcatgcg ctcgatgccg atgaacgccg anntcctgat cggtccggtc gattc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAESAAQRD SNTSIASTKI ERTKAERLAR EQQIARTVPS LASISLTQPR PFTLRDSGQN GAMTFLRDVD FRIVNNLGFF IHQLSATLVP TQAGAPIVFD DPTSFEIDVH EGTVTLDNAK LTALFNTYIF GYRNAPLRKL AVSAGDGVIH LQGEMQRDGW VPFSLTGTLA IRDGSQLVFH PTGVRVSGIN AQPVMRAANV KMADLLKVQT PIAQLAGDDL VMAVDKLMPP PRLKLTITAV RVTPAGLDLK LDDGTHAGFA MPANAPQQAM YIRGGDVKFM RSMPMNADIL IGPVDSSKRE QTFVFDLYHY RDQVSAGYFN FDESGAMAIR MPSYTGPASG ALLGNAAARL NDSFLAAQQT ALRDVRQHWE AYALAADAMR PGMQKVAMRR TSTTPFNERH VSNRNPTIHL RNVDFNLSGD IGFHVEDLDV ELVSRRPGEP VDLDDPNQYD IRILGGTVVE SWKAMSALFN NYLLDYSPRS LNDLQLSADG QNLRVQGGIK LWNHVPGVWL PTDMKGSLTV LDDRHLAFKP SQVSVLGIPQ ATLLRSLGIE LASLTPLRRR GAELRGDTLV LDQYTVFPPP VLNGKLGQAT VERDGLRLTF RRAAGAAAPK RPQIDAPSYM WMEGGDMKMF NVLELNVRAL IRNSAEAGPM RFDLYGYRSQ VAQGSVRMLP DGTLVVDIGQ KNPLASR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggaatcg gccgcgcagc gcgacagcaa cacgtccatc gcatcgacga agatcgaacg caccaaggcc gagcgactcg cgcgcgaaca gcagatcgcg cgcaccgtgc cgtcgctcgc atcgatcagc ctcacgcagc cgcgcccgtt cacgctgcgc gactcgggcc agaacggcgc gatgacgttc ctgcgcgacg tcgatttccg catcgtcaac aacctcggct tcttcatcca ccagttgtcg gccacgctcg tgccgacgca ggccggcgcg ccgatcgtct tcgacgatcc gacgagcttc gagatcgacg tacatgaagg cacggtgacg ctcgacaacg cgaagctgac cgccctcttc aacacctaca tcttcggcta tcgcaacgcg ccgctgcgca agctcgcagt gtcggccggc gacggcgtga tccatctgca aggcgagatg cagcgcgacg gctgggtacc gttctcgctg accggcacgc tcgcgatccg cgacggcagc cagctcgtgt tccacccgac cggcgtacgc gtgtcgggta tcaacgcgca gccggtgatg cgcgcggcca acgtgaagat ggccgacctg ctgaaggtgc agaccccgat cgcgcaactt gccggcgacg atctcgtgat ggcggtcgac aagctgatgc cgccaccgcg cctgaagctc acgatcaccg ccgtgcgcgt gacgccggca ggcctcgacc tgaagctcga cgacggcacg catgccggct tcgcgatgcc ggcgaatgcc ccgcagcagg cgatgtacat ccgcggcggc gacgtgaagt tcatgcgctc gatgccgatg aacgccgaca tcctgatcgg tccggtcgat tcgtcgaagc gcgagcagac gttcgtgttc gacctgtatc actatcgcga ccaggtgtcg gccggttact tcaacttcga cgaaagcggc gcgatggcga tccggatgcc ttcgtatacg ggcccggcga gcggcgcgct gctcggcaac gcggcggcgc gcctgaacga cagcttcctc gccgcgcagc agactgcgct gcgcgacgtg cgccagcact gggaagcgta cgcgctcgcg gcggatgcga tgcggcccgg catgcagaag gtcgcgatgc ggcgcacgtc gacgacgccg ttcaacgaac ggcacgtgtc gaaccgcaac ccgacgatcc atctgcgcaa cgtcgacttc aacctgtccg gcgatatcgg ctttcatgtc gaggatctcg acgtggaact cgtgtcgagg cggcccggcg aaccggtcga tctcgacgat ccgaaccagt acgacatccg cattctcggc ggcacggtcg tcgaatcgtg gaaggcgatg tcggcgctct tcaacaacta cctgctcgac tattcgccgc gttcgctgaa cgacctgcaa ctgagcgcgg acgggcagaa cctgcgcgtc cagggcggca tcaagctgtg gaatcacgtg ccgggggtct ggctgccgac cgacatgaag ggctcgctga cggtgctcga cgaccggcac ctcgccttca agccgtcgca ggtgtcggtg ctcggcatcc cgcaggcgac gctgctgcgc tcgctcggca tcgagctcgc gtcgctgacg ccgttgcgcc gacgcggcgc ggaactgcgc ggcgacacgc tcgtgctcga ccagtacacc gtgttcccgc cgcccgtgct gaacggcaag ctcggccagg caacggtcga acgcgacggc ttgcgtctca cgttccggcg cgcagcgggt gcagccgcgc cgaagcggcc gcagatcgat gcgccgagct acatgtggat ggaaggcggc gacatgaaga tgttcaacgt gctcgagctg aacgtgcgcg cgttgatccg gaattcggcc gaggcgggcc cgatgcggtt cgacctgtac ggataccgca gccaggtcgc gcagggctcg gtgcggatgc tgccggacgg cacgctggtc gtcgacatcg ggcagaagaa tccgctcgcg tcgcgctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BuceA.17071.a.A2.PS01483
PURIFICATION DATe: 5/1/2012
CONCENTRATION: 9.98mg/ml
OBSERVED MW: 78kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 44
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAESAAQRD SNTSIASTKI ERTKAERLAR EQQIARTVPS LASISLTQPR PFTLRDSGQN GAMTFLRDVD FRIVNNLGFF IHQLSATLVP TQAGAPIVFD DPTSFEIDVH EGTVTLDNAK LTALFNTYIF GYRNAPLRKL AVSAGDGVIH LQGEMQRDGW VPFSLTGTLA IRDGSQLVFH PTGVRVSGIN AQPVMRAANV KMADLLKVQT PIAQLAGDDL VMAVDKLMPP PRLKLTITAV RVTPAGLDLK LDDGTHAGFX MPANAPQXXM YIRGGDVKFM RSMPMNAXXL IGPVD
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcggaa tcggccgcgc agcgcgacag caacacgtcc atcgcatcga cgaagatcga acgcaccaag gccgagcgac tcgcgcgcga acagcagatc gcgcgcaccg tgccgtcgct cgcatcgatc agcctcacgc agccgcgccc gttcacgctg cgcgactcgg gccagaacgg cgcgatgacg ttcctgcgcg acgtcgattt ccgcatcgtc aacaacctcg gcttcttcat ccaccagttg tcggccacgc tcgtgccgac gcaggccggc gcgccgatcg tcttcgacga tccgacgagc ttcgagatcg acgtacatga aggcacggtg acgctcgaca acgcgaagct gaccgccctc ttcaacacct acatcttcgg ctatcgcaac gcgccgctgc gcaagctcgc agtgtcggcc ggcgacggcg tgatccatct gcaaggcgag atgcagcgcg acggctgggt accgttctcg ctgaccggca cgctcgcgat ccgcgacggc agccagctcg tgttccaccc gaccggcgta cgcgtgtcgg gtatcaacgc gcagccggtg atgcgcgcgg ccaacgtgaa gatggccgac ctgctgaagg tgcagacccc gatcgcgcaa cttgccggcg acgatctcgt gatggcggtc gacaagctga tgccgccacc gcgcctgaag ctcacgatca ccgccgtgcg cgtgacgccn gcaggnctcg acctgaagct cgacgacggc acgcatgccg gcttcncgat gccggcgaat gccccgcagc nggngatgta catccgcggc ggcgacgtga agttcatgcg ctcgatgccg atgaacgccg anntcctgat cggtccggtc gattc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAESAAQRD SNTSIASTKI ERTKAERLAR EQQIARTVPS LASISLTQPR PFTLRDSGQN GAMTFLRDVD FRIVNNLGFF IHQLSATLVP TQAGAPIVFD DPTSFEIDVH EGTVTLDNAK LTALFNTYIF GYRNAPLRKL AVSAGDGVIH LQGEMQRDGW VPFSLTGTLA IRDGSQLVFH PTGVRVSGIN AQPVMRAANV KMADLLKVQT PIAQLAGDDL VMAVDKLMPP PRLKLTITAV RVTPAGLDLK LDDGTHAGFA MPANAPQQAM YIRGGDVKFM RSMPMNADIL IGPVDSSKRE QTFVFDLYHY RDQVSAGYFN FDESGAMAIR MPSYTGPASG ALLGNAAARL NDSFLAAQQT ALRDVRQHWE AYALAADAMR PGMQKVAMRR TSTTPFNERH VSNRNPTIHL RNVDFNLSGD IGFHVEDLDV ELVSRRPGEP VDLDDPNQYD IRILGGTVVE SWKAMSALFN NYLLDYSPRS LNDLQLSADG QNLRVQGGIK LWNHVPGVWL PTDMKGSLTV LDDRHLAFKP SQVSVLGIPQ ATLLRSLGIE LASLTPLRRR GAELRGDTLV LDQYTVFPPP VLNGKLGQAT VERDGLRLTF RRAAGAAAPK RPQIDAPSYM WMEGGDMKMF NVLELNVRAL IRNSAEAGPM RFDLYGYRSQ VAQGSVRMLP DGTLVVDIGQ KNPLASR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGAATCG GCCGCGCAGC GCGACAGCAA CACGTCCATC GCATCGACGA AGATCGAACG CACCAAGGCC GAGCGACTCG CGCGCGAACA GCAGATCGCG CGCACCGTGC CGTCGCTCGC ATCGATCAGC CTCACGCAGC CGCGCCCGTT CACGCTGCGC GACTCGGGCC AGAACGGCGC GATGACGTTC CTGCGCGACG TCGATTTCCG CATCGTCAAC AACCTCGGCT TCTTCATCCA CCAGTTGTCG GCCACGCTCG TGCCGACGCA GGCCGGCGCG CCGATCGTCT TCGACGATCC GACGAGCTTC GAGATCGACG TACATGAAGG CACGGTGACG CTCGACAACG CGAAGCTGAC CGCCCTCTTC AACACCTACA TCTTCGGCTA TCGCAACGCG CCGCTGCGCA AGCTCGCAGT GTCGGCCGGC GACGGCGTGA TCCATCTGCA AGGCGAGATG CAGCGCGACG GCTGGGTACC GTTCTCGCTG ACCGGCACGC TCGCGATCCG CGACGGCAGC CAGCTCGTGT TCCACCCGAC CGGCGTACGC GTGTCGGGTA TCAACGCGCA GCCGGTGATG CGCGCGGCCA ACGTGAAGAT GGCCGACCTG CTGAAGGTGC AGACCCCGAT CGCGCAACTT GCCGGCGACG ATCTCGTGAT GGCGGTCGAC AAGCTGATGC CGCCACCGCG CCTGAAGCTC ACGATCACCG CCGTGCGCGT GACGCCGGCA GGCCTCGACC TGAAGCTCGA CGACGGCACG CATGCCGGCT TCGCGATGCC GGCGAATGCC CCGCAGCAGG CGATGTACAT CCGCGGCGGC GACGTGAAGT TCATGCGCTC GATGCCGATG AACGCCGACA TCCTGATCGG TCCGGTCGAT TCGTCGAAGC GCGAGCAGAC GTTCGTGTTC GACCTGTATC ACTATCGCGA CCAGGTGTCG GCCGGTTACT TCAACTTCGA CGAAAGCGGC GCGATGGCGA TCCGGATGCC TTCGTATACG GGCCCGGCGA GCGGCGCGCT GCTCGGCAAC GCGGCGGCGC GCCTGAACGA CAGCTTCCTC GCCGCGCAGC AGACTGCGCT GCGCGACGTG CGCCAGCACT GGGAAGCGTA CGCGCTCGCG GCGGATGCGA TGCGGCCCGG CATGCAGAAG GTCGCGATGC GGCGCACGTC GACGACGCCG TTCAACGAAC GGCACGTGTC GAACCGCAAC CCGACGATCC ATCTGCGCAA CGTCGACTTC AACCTGTCCG GCGATATCGG CTTTCATGTC GAGGATCTCG ACGTGGAACT CGTGTCGAGG CGGCCCGGCG AACCGGTCGA TCTCGACGAT CCGAACCAGT ACGACATCCG CATTCTCGGC GGCACGGTCG TCGAATCGTG GAAGGCGATG TCGGCGCTCT TCAACAACTA CCTGCTCGAC TATTCGCCGC GTTCGCTGAA CGACCTGCAA CTGAGCGCGG ACGGGCAGAA CCTGCGCGTC CAGGGCGGCA TCAAGCTGTG GAATCACGTG CCGGGGGTCT GGCTGCCGAC CGACATGAAG GGCTCGCTGA CGGTGCTCGA CGACCGGCAC CTCGCCTTCA AGCCGTCGCA GGTGTCGGTG CTCGGCATCC CGCAGGCGAC GCTGCTGCGC TCGCTCGGCA TCGAGCTCGC GTCGCTGACG CCGTTGCGCC GACGCGGCGC GGAACTGCGC GGCGACACGC TCGTGCTCGA CCAGTACACC GTGTTCCCGC CGCCCGTGCT GAACGGCAAG CTCGGCCAGG CAACGGTCGA ACGCGACGGC TTGCGTCTCA CGTTCCGGCG CGCAGCGGGT GCAGCCGCGC CGAAGCGGCC GCAGATCGAT GCGCCGAGCT ACATGTGGAT GGAAGGCGGC GACATGAAGA TGTTCAACGT GCTCGAGCTG AACGTGCGCG CGTTGATCCG GAATTCGGCC GAGGCGGGCC CGATGCGGTT CGACCTGTAC GGATACCGCA GCCAGGTCGC GCAGGGCTCG GTGCGGATGC TGCCGGACGG CACGCTGGTC GTCGACATCG GGCAGAAGAA TCCGCTCGCG TCGCGCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA