BuceA.16001.a

Metallo-beta-lactamase superfamily protein

CENTER ID: BuceA.16001.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.16001.a.B1.GE37739 Full length( BuceA.16001.a ) 1 258
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2254
RefSeq: YP_002231292.1
UniProt: B4ED87

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRFASLGSGS EGNALVVEAS SGTTTTRVLL DCGFSAKEVE RRLGRLNLGV ADLDAILITH EHSDHVGSAL TLARRASLPL YMSWGTARAV GADEADVDLH VLWGDETAAI RDLAVMPYTV PHDAREPLQF VFMDGNRRLG VLTDVGMATP HITAVLSGCD ALVLESNHDT AMLAASRYPQ SLKARIGGNH GHLSNDAAAE ILASLERSRL QHLVAAHLSQ QNNLPELARQ AFGGVLGTDG EDVIVASQDA GFDWLMLG
NT Sequence
GTGCGATTCG CCAGCCTCGG AAGCGGCAGC GAAGGCAACG CGCTGGTCGT CGAGGCCTCG AGCGGCACGA CGACCACCCG TGTGCTGCTC GACTGCGGCT TTTCCGCCAA GGAGGTCGAG CGCCGGCTCG GCCGACTGAA TCTCGGCGTC GCCGATCTCG ACGCGATCCT CATTACCCAT GAACACAGCG ACCACGTCGG CAGCGCGCTG ACGCTCGCGC GCCGCGCGTC GCTGCCGCTC TACATGAGCT GGGGCACCGC CCGCGCGGTC GGCGCGGACG AGGCCGATGT CGATCTTCAC GTGCTGTGGG GCGACGAGAC GGCCGCGATA CGCGATCTGG CCGTGATGCC CTATACGGTC CCCCACGACG CGCGGGAGCC GCTCCAGTTC GTCTTCATGG ACGGAAACCG CCGGCTCGGC GTGCTGACGG ACGTCGGGAT GGCCACGCCG CACATCACGG CCGTCCTGAG CGGCTGTGAC GCGCTGGTGC TCGAATCCAA CCACGATACC GCGATGCTGG CCGCCAGCCG CTATCCGCAG TCGCTGAAGG CGCGGATCGG CGGCAACCAC GGCCACCTGA GCAACGACGC GGCGGCCGAG ATTCTCGCGT CGCTCGAACG CAGCCGTCTC CAGCACCTGG TGGCGGCCCA TCTGAGCCAG CAGAACAACC TGCCAGAACT GGCCCGCCAG GCGTTTGGCG GTGTGCTCGG GACGGACGGG GAAGACGTGA TCGTGGCCTC GCAGGACGCG GGCTTCGACT GGCTGATGCT CGGA
Details for BuceA.16001.a.B1.GE37739
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/7/2014
EXPECTED MW: 28kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV RFASLGSGSE GNALVVEASS GTTTTRVLLD CGFSAKEVER RLGRLNLGVA DLDAILITHE HSDHVGSALT LARRASLPLY MSWGTARAVG ADEADVDLHV LWGDETAAIR DLAVMPYTVP HDAREPLQFV FMDGNRRLGV LTDVGMATPH ITAVLSGCDA LVLESNHDTA MLAASRYPQS LKARIGGNHG HLSNDAAAEI LASLERSRLQ HLVAAHLSQQ NNLPELARQA FGGVLGTDGE DVIVASQDAG FDWLMLG
Validated NT Sequence
cnctanatat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc gattcgccag cctcggaagc ggcagcgaag gcaacgcgct ggtcgtcgag gcctcgagcg gcacgacgac cacccgtgtg ctgctcgact gcggcttttc cgccaaggag gtcgagcgcc ggctcggccg actgaatctc ggcgtcgccg atctcgacgc gatcctcatt acccatgaac acagcgacca cgtcggcagc gcgctgacgc tcgcgcgccg cgcgtcgctg ccgctctaca tgagctgggg caccgcccgc gcggtcggcg cggacgaggc cgatgtcgat cttcacgtgc tgtggggcga cgagacggcc gcgatacgcg atctggccgt gatgccctat acggtccccc acgacgcgcg ggagccgctc cagttcgtct tcatggacgg aaaccgccgg ctcggcgtgc tgacggacgt cgggatggcc acgccgcaca tcacggccgt cctgagcggc tgtgacgcgc tggtgctcga atccaaccac gataccgcga tgctggccgc cagccgctat ccgcagtcgc tgaaggcgcg gatcggcggc aaccacggcc acctgagcaa cgacgcggcg gccgagattc tcgcgtcgct cgaacgcagc cgtctccagc acctggtggc ggcccatctg agccagcaga acaacctgcc agaactggcc cgccaggcgt ttggcggtgt gctcgggacg gacggggaag acgtgatcgt ggcctcgcag gacgcgggct tcgactggct gatgctcgga taagtgagta agataggatc cggctgctaa nnacccccca aaaagnga
Expected Protein Sequence
MAHHHHHHMR FASLGSGSEG NALVVEASSG TTTTRVLLDC GFSAKEVERR LGRLNLGVAD LDAILITHEH SDHVGSALTL ARRASLPLYM SWGTARAVGA DEADVDLHVL WGDETAAIRD LAVMPYTVPH DAREPLQFVF MDGNRRLGVL TDVGMATPHI TAVLSGCDAL VLESNHDTAM LAASRYPQSL KARIGGNHGH LSNDAAAEIL ASLERSRLQH LVAAHLSQQN NLPELARQAF GGVLGTDGED VIVASQDAGF DWLMLG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgat tcgccagcct cggaagcggc agcgaaggca acgcgctggt cgtcgaggcc tcgagcggca cgacgaccac ccgtgtgctg ctcgactgcg gcttttccgc caaggaggtc gagcgccggc tcggccgact gaatctcggc gtcgccgatc tcgacgcgat cctcattacc catgaacaca gcgaccacgt cggcagcgcg ctgacgctcg cgcgccgcgc gtcgctgccg ctctacatga gctggggcac cgcccgcgcg gtcggcgcgg acgaggccga tgtcgatctt cacgtgctgt ggggcgacga gacggccgcg atacgcgatc tggccgtgat gccctatacg gtcccccacg acgcgcggga gccgctccag ttcgtcttca tggacggaaa ccgccggctc ggcgtgctga cggacgtcgg gatggccacg ccgcacatca cggccgtcct gagcggctgt gacgcgctgg tgctcgaatc caaccacgat accgcgatgc tggccgccag ccgctatccg cagtcgctga aggcgcggat cggcggcaac cacggccacc tgagcaacga cgcggcggcc gagattctcg cgtcgctcga acgcagccgt ctccagcacc tggtggcggc ccatctgagc cagcagaaca acctgccaga actggcccgc caggcgtttg gcggtgtgct cgggacggac ggggaagacg tgatcgtggc ctcgcaggac gcgggcttcg actggctgat gctcggatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at