BuceA.12800.x

LysR family regulatory protein

CENTER ID: BuceA.12800.x
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12800.x.A1.GE31879 Full length( BuceA.12800.x ) 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.7415
RefSeq: YP_002154084.1
UniProt: B4EPA1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRTLHAMSV FSHVARLGSF TAAADMLGIS AASASTLVRR LEEYLGVTLL QRSTRCVRLT TEGEQYQEHC QRILGEIELM QQQLSGAGKI ARGRLAVDLD QEVAHSVLPF LPVFRANHPE VGLRVDVGGD ADGLIANGVD CAVVVGQLAD SSLRSRRVGQ FHAVTVASPD YLKARGTPVD PDGLHCHDVI HYTPRRFGPV REFRYTVDGA ETRLKLAERI SVNDVRSAVR YAVEGVGIAQ VSERMVAEEL ATGTLVSILG EYGPPPLPIS VLYPDRRQVP MSIRAFIDWM QTQLRDLEIA SFDHTSAPNG RRDAAPYWTS NSERPFITTG FQQLSATA
NT Sequence
ATGGACCGTA CCCTGCACGC GATGTCAGTG TTTAGTCACG TCGCCCGCCT CGGCAGTTTC ACCGCAGCGG CTGATATGCT CGGTATCAGT GCAGCGTCGG CCAGCACGCT GGTTCGCAGA CTGGAAGAAT ATCTCGGCGT AACGTTGCTT CAGCGATCGA CCCGCTGTGT CAGGCTCACG ACGGAGGGCG AGCAGTATCA GGAGCACTGC CAGCGCATTC TCGGCGAGAT CGAACTGATG CAGCAGCAAC TGAGCGGCGC CGGGAAGATC GCGCGCGGAC GGTTGGCGGT GGATCTCGAT CAGGAAGTCG CGCATTCGGT GTTGCCGTTC CTGCCGGTGT TCCGTGCGAA CCATCCGGAA GTGGGCTTGC GGGTCGACGT CGGCGGCGAC GCGGACGGCC TGATCGCGAA CGGCGTGGAC TGCGCGGTCG TGGTCGGCCA GCTGGCGGAC TCGTCGCTGC GCAGCCGGCG CGTCGGGCAG TTTCATGCGG TGACGGTGGC GAGTCCCGAC TATCTGAAAG CGCGCGGCAC GCCGGTCGAC CCCGACGGCC TCCATTGCCA CGACGTGATC CATTACACGC CGCGGCGCTT CGGTCCGGTC CGAGAGTTCC GTTACACCGT GGACGGCGCC GAGACGCGGC TCAAGCTGGC GGAGCGGATT TCCGTCAATG ATGTGCGTTC GGCCGTTCGA TATGCGGTCG AAGGCGTCGG CATCGCGCAG GTGAGCGAAC GGATGGTCGC GGAAGAGTTG GCGACGGGTA CGCTCGTCAG CATTCTCGGC GAGTACGGCC CGCCGCCGCT GCCGATTTCG GTGCTGTATC CGGATCGACG TCAGGTGCCG ATGTCGATAC GCGCGTTCAT CGACTGGATG CAGACCCAGC TTCGCGATCT GGAAATCGCC AGCTTCGACC ACACGTCGGC GCCGAACGGC AGGCGCGACG CGGCACCGTA CTGGACGTCG AACAGCGAGC GGCCGTTCAT TACAACGGGC TTCCAGCAGC TGTCGGCTAC GGCG
Details for BuceA.12800.x.A1.GE31879
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMDRTLHA MSVFSHVARL GSFTAAADML GISAASASTL VRRLEEYLGV TLLQRSTRCV RLTTEGEQYQ EHCQRILGEI ELMQQQLSGA GKIARGRLAV DLDQEVAHSV LPFLPVFRAN HPEVGLRVDV GGDADGLIAN GVDCAVVVGQ LADSSLRSRR VGQFHAVTVA SPDYLKARGT PVDPDGLHCH DVIHYTPRRF GPVREFRYTV DGAETRLKLA ERISVNDVRS AVRYAVEGVG IAQVSERMVA EELATGTLVS ILGEYGPPPL PISVLYPDRR QVPMSIRAFI DWMQTQLRDL EIASFDHTSA PNGRRDAAPY WTSNSERPFI TTGFQQLSAT A
Validated NT Sequence
ttattacgcc gtagccgaca gctgctggaa gcccgttgta atgaacggcc gctcgctgtt cgacgtccag tacggtgccg cgtcgcgcct gccgttcggc gccgacgtgt ggtcgaagct ggcgatttcc agatcgcgaa gctgggtctg catccagtcg atgaacgcgc gtatcgacat cggcacctga cgtcgatccg gatacagcac cgaaatcggc agcggcggcg ggccgtactc gccgagaatg ctgacgagcg tacccgtcgc caactcttcc gcgaccatcc gttcgctcac ctgcgcgatg ccgacgcctt cgaccgcata tcgaacggcc gaacgcacat cattgacgga aatccgctcc gccagcttga gccgcgtctc ggcgccgtcc acggtgtaac ggaactctcg gaccggaccg aagcgccgcg gcgtgtaatg gatcacgtcg tggcaatgga ggccgtcggg gtcgaccggc gtgccgcgcg ctttcagata gtcgggactc gccaccgtca ccgcatgaaa ctgcccgacg cgccggctgc gcagcgacga gtccgccagc tggccgacca cgaccgcgca gtccacgccg ttcgcgatca ggccgtccgc gtcgccgccg acgtcgaccc gcaagcccac ttccggatgg ttcgcacgga acaccggcag gaacggcaac accgaatgcg cgacttcctg atcgagatcc accgccaacc gtccgcgcgc gatcttcccg gcgccgctca gttgctgctg catcagttcg atctcgccga gaatgcgctg gcagtgctcc tgatactgct cgccctccgt cgtgagcctg acacagcggg tcgatcgctg aagcaacgtt acgccgagat attcttccag tctgcgaacc agcgtgctgg ccgacgctgc actgataccg agcatatcag ccgctgcggt gaaactgccg aggcgggcga cgtgactaaa cactgacatc gcgtgcaggg tacggtccat cgaaccagga ccctgggtct gagcttccag ggtacccata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRTLHAMS VFSHVARLGS FTAAADMLGI SAASASTLVR RLEEYLGVTL LQRSTRCVRL TTEGEQYQEH CQRILGEIEL MQQQLSGAGK IARGRLAVDL DQEVAHSVLP FLPVFRANHP EVGLRVDVGG DADGLIANGV DCAVVVGQLA DSSLRSRRVG QFHAVTVASP DYLKARGTPV DPDGLHCHDV IHYTPRRFGP VREFRYTVDG AETRLKLAER ISVNDVRSAV RYAVEGVGIA QVSERMVAEE LATGTLVSIL GEYGPPPLPI SVLYPDRRQV PMSIRAFIDW MQTQLRDLEI ASFDHTSAPN GRRDAAPYWT SNSERPFITT GFQQLSATA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccgtacc ctgcacgcga tgtcagtgtt tagtcacgtc gcccgcctcg gcagtttcac cgcagcggct gatatgctcg gtatcagtgc agcgtcggcc agcacgctgg ttcgcagact ggaagaatat ctcggcgtaa cgttgcttca gcgatcgacc cgctgtgtca ggctcacgac ggagggcgag cagtatcagg agcactgcca gcgcattctc ggcgagatcg aactgatgca gcagcaactg agcggcgccg ggaagatcgc gcgcggacgg ttggcggtgg atctcgatca ggaagtcgcg cattcggtgt tgccgttcct gccggtgttc cgtgcgaacc atccggaagt gggcttgcgg gtcgacgtcg gcggcgacgc ggacggcctg atcgcgaacg gcgtggactg cgcggtcgtg gtcggccagc tggcggactc gtcgctgcgc agccggcgcg tcgggcagtt tcatgcggtg acggtggcga gtcccgacta tctgaaagcg cgcggcacgc cggtcgaccc cgacggcctc cattgccacg acgtgatcca ttacacgccg cggcgcttcg gtccggtccg agagttccgt tacaccgtgg acggcgccga gacgcggctc aagctggcgg agcggatttc cgtcaatgat gtgcgttcgg ccgttcgata tgcggtcgaa ggcgtcggca tcgcgcaggt gagcgaacgg atggtcgcgg aagagttggc gacgggtacg ctcgtcagca ttctcggcga gtacggcccg ccgccgctgc cgatttcggt gctgtatccg gatcgacgtc aggtgccgat gtcgatacgc gcgttcatcg actggatgca gacccagctt cgcgatctgg aaatcgccag cttcgaccac acgtcggcgc cgaacggcag gcgcgacgcg gcaccgtact ggacgtcgaa cagcgagcgg ccgttcatta caacgggctt ccagcagctg tcggctacgg cgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa