SSGCID: Target Page

BuceA.12800.w

LysR family regulatory protein

CENTER ID:	BuceA.12800.w
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.12800.w.A1.GE31878	Full length( BuceA.12800.w )		1	333

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.4445
RefSeq:	YP_002233364.1
UniProt:	B4EM43

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDNPIRAMRI FTRIVEMNSF TRAAQALGIS RATATRTVQE LEAALGMPLL VRTTRALRPT PEGDAYYRRC VSITADVDEL EASIRGAALH PSGPLRVELP ASVAGAIVLP ALGEFHSRHP DLELMLAVAG RAGDRIGDAI DCSVRLGALP DSSLVARRLG ALERVTCASP AYLDLFGVPR TPDDLGAHRA ICGSHVFGQR PAELEFMVDD AVRKVRLDGI VSVDDEQAYL ACGVHGLGLI QPTRVAAQPL IEAGRLREVL PCWQPDALAV SVVYVKRPHV SPGVRAFVDW IAERFVQAGK EHTGLAAAGA AGWRPAQDAA APCDRYSGLV AAA

NT Sequence

GTGGACAATC CGATCCGCGC GATGCGCATC TTCACGCGCA TCGTCGAAAT GAACAGTTTT ACGCGCGCGG CGCAGGCGCT CGGCATTTCG CGCGCCACCG CCACGCGCAC CGTGCAGGAG CTCGAAGCGG CGCTCGGCAT GCCGCTGCTG GTGCGCACGA CGCGCGCGCT GCGGCCGACG CCCGAAGGCG ACGCGTATTA CCGGCGCTGC GTCAGCATCA CGGCGGACGT CGACGAGCTC GAAGCGAGCA TCCGCGGCGC CGCGCTGCAT CCGAGCGGGC CGCTGCGCGT CGAATTGCCG GCGTCGGTGG CGGGCGCGAT CGTGTTGCCG GCGCTCGGCG AATTTCATAG CCGGCATCCC GATCTGGAGC TGATGCTCGC CGTGGCCGGG CGCGCGGGCG ACCGCATCGG CGACGCGATC GATTGCAGCG TCCGGCTCGG TGCGCTGCCC GATTCGTCGC TCGTCGCGCG CCGGCTCGGC GCGCTCGAGC GCGTGACCTG CGCGAGCCCC GCGTATCTCG ACCTCTTCGG CGTGCCGCGC ACACCCGACG ATCTGGGCGC GCATCGTGCG ATTTGCGGAT CGCACGTGTT CGGCCAGCGT CCGGCGGAGC TCGAGTTCAT GGTGGACGAC GCGGTGCGCA AGGTGCGGCT CGACGGCATC GTCAGCGTGG ACGACGAGCA GGCGTATCTC GCCTGCGGCG TGCACGGCCT CGGACTGATC CAGCCGACGC GCGTCGCCGC GCAGCCGCTG ATCGAGGCGG GACGGCTGCG GGAGGTGCTG CCGTGTTGGC AACCCGATGC GCTCGCCGTG TCGGTCGTCT ACGTGAAGCG CCCGCATGTG TCGCCCGGCG TGCGCGCGTT CGTCGACTGG ATCGCGGAGC GTTTCGTGCA GGCCGGGAAG GAACACACAG GGCTGGCGGC GGCGGGGGCG GCAGGGTGGC GGCCGGCGCA GGATGCCGCC GCTCCCTGCG ACCGCTACAG CGGACTGGTC GCGGCAGCC

Details for BuceA.12800.w.A1.GE31878

HARVESTED ON:	3/22/2011
SEQUENCED ON:	3/25/2011
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	77
PERCENT COVERAGE:	109

Validated AA Sequence

MGTLEAQTQG PGSMVDNPIR AMRIFTRIVE MNSFTRAAQA LGISRATATR TVQELEAALG MPLLVRTTRA LRPTPEGDAY YRRCVSITAD VDELEASIRG AALHPSGPLR VELPASVAGA IVLPALGEFH SRHPDLELML AVAGRAGDRI GDAIDCSVRL GALPDSSLVA RRLGALERVT CASPAYLDLF GVPRTPDDLG AHRAICGSHV FGQRPAXLEF MVDDAVRKVR LDGIVSVDDE XAYLACGVHG LGLIQPXRVA AQPXXXXXRL RXXAAVXATR CARRVGRLRE APXCVXRXXR VXRLDXXXFV XAXXXXXRXX GXGGXRXAXX XQXAXXXXXX LXXXWXXXXX XXXXXXXXXX XLXXXXXXXX XXXXXXXXXX XXXXXXXXX

Validated NT Sequence

gcggnnnagc catgtnnnnn nnnnnntncc nnngnnnnnn nnnnnncacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggtg gacaatccga tccgcgcgat gcgcatcttc acgcgcatcg tcgaaatgaa cagttttacg cgcgcggcgc aggcgctcgg catttcgcgc gccaccgcca cgcgcaccgt gcaggagctc gaagcggcgc tcggcatgcc gctgctggtg cgcacgacgc gcgcgctgcg gccgacgccc gaaggcgacg cgtattaccg gcgctgcgtc agcatcacgg cggacgtcga cgagctcgaa gcgagcatcc gcggcgccgc gctgcatccg agcgggccgc tgcgcgtcga attgccggcg tcggtggcgg gcgcgatcgt gttgccggcg ctcggcgaat ttcatagccg gcatcccgat ctggagctga tgctcgccgt ggccgggcgc gcgggcgacc gcatcggcga cgcgatcgat tgcagcgtcc ggctcggtgc gctgcccgat tcgtcgctcg tcgcgcgccg gctcggcgcg ctcgagcgcg tgacctgcgc gagccccgcg tatctcgacc tcttcggcgt gccgcgcaca cccgacgatc tgggcgcgca tcgtgcgatt tgcggatcgc acgtgttcgg ccagcgtccg gcgganctcg agttcatggt ggacgacgcg gtgcgcaagg tgcggctcga cggcatcgtc agcgtggacg acgagcangc gtatctcgcc tgcggcgtgc acggcctcgg actgatccag ccgangcgcg tcgccgcgca gccgntgann nnnnngnnnc ggctgcgggn nngngctgcc gtgntggcaa cccgatgcgc tcgccgtgtc ggtcgtctac gtgaagcncc cncatgtgtc ncccggngtg nncgcgttcn tcgactggat cncnnnncgt tcgtgcangc cnggnannna nnnnnnaggg ntngnggcng cgggggnngn agggnggcng ncnggnnnca gnatgccncc nctnccntgc nacnnctncn nnngnantgg nnnnnnnnnn nncntaanna aanngncnnn annnnntnnn nnnnnnngct tnnnncnnnn nnnnnnnnnn nnnnnnnnna nnnncnaaan nnnnnnnnnn nnnnnnnnnn cnnnnnnnnn nngnnnnnnn nnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVDNPIRAM RIFTRIVEMN SFTRAAQALG ISRATATRTV QELEAALGMP LLVRTTRALR PTPEGDAYYR RCVSITADVD ELEASIRGAA LHPSGPLRVE LPASVAGAIV LPALGEFHSR HPDLELMLAV AGRAGDRIGD AIDCSVRLGA LPDSSLVARR LGALERVTCA SPAYLDLFGV PRTPDDLGAH RAICGSHVFG QRPAELEFMV DDAVRKVRLD GIVSVDDEQA YLACGVHGLG LIQPTRVAAQ PLIEAGRLRE VLPCWQPDAL AVSVVYVKRP HVSPGVRAFV DWIAERFVQA GKEHTGLAAA GAAGWRPAQD AAAPCDRYSG LVAAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggacaat ccgatccgcg cgatgcgcat cttcacgcgc atcgtcgaaa tgaacagttt tacgcgcgcg gcgcaggcgc tcggcatttc gcgcgccacc gccacgcgca ccgtgcagga gctcgaagcg gcgctcggca tgccgctgct ggtgcgcacg acgcgcgcgc tgcggccgac gcccgaaggc gacgcgtatt accggcgctg cgtcagcatc acggcggacg tcgacgagct cgaagcgagc atccgcggcg ccgcgctgca tccgagcggg ccgctgcgcg tcgaattgcc ggcgtcggtg gcgggcgcga tcgtgttgcc ggcgctcggc gaatttcata gccggcatcc cgatctggag ctgatgctcg ccgtggccgg gcgcgcgggc gaccgcatcg gcgacgcgat cgattgcagc gtccggctcg gtgcgctgcc cgattcgtcg ctcgtcgcgc gccggctcgg cgcgctcgag cgcgtgacct gcgcgagccc cgcgtatctc gacctcttcg gcgtgccgcg cacacccgac gatctgggcg cgcatcgtgc gatttgcgga tcgcacgtgt tcggccagcg tccggcggag ctcgagttca tggtggacga cgcggtgcgc aaggtgcggc tcgacggcat cgtcagcgtg gacgacgagc aggcgtatct cgcctgcggc gtgcacggcc tcggactgat ccagccgacg cgcgtcgccg cgcagccgct gatcgaggcg ggacggctgc gggaggtgct gccgtgttgg caacccgatg cgctcgccgt gtcggtcgtc tacgtgaagc gcccgcatgt gtcgcccggc gtgcgcgcgt tcgtcgactg gatcgcggag cgtttcgtgc aggccgggaa ggaacacaca gggctggcgg cggcgggggc ggcagggtgg cggccggcgc aggatgccgc cgctccctgc gaccgctaca gcggactggt cgcggcagcc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa