SSGCID: Target Page

BuceA.12800.v

LysR family regulatory protein

CENTER ID:	BuceA.12800.v
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.12800.v.A1.GE31877	Full length( BuceA.12800.v )		1	303

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.12800.v.A1.PW31790	Full length( BuceA.12800.v )		1	303

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.5574
RefSeq:	YP_002234400.1
UniProt:	B4ELU7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDHLQAMRIF ARVAHLGSFT KAAEQLQLPR PTVSNAVQYL EKHLKIRLLQ RTTRRVALTA EGATYYERCV RLLADLDDAE TLFEDAGASP RGVIRVDLPE RFALNQVIPA LPDFHARYPD LRVAISTTDR FVDLVADGID CAVRVGALSD TSLVARRVGE MAQINCASPA YLARHGTPRS PDDLPDHVAV GYFSSRTGRE LDWEYADMDS GEMHTVKMHS VVSVNSSQAY LACCLAGLGL IQAPRDGLAP LLADGSLVEV LPEWRAGPLP VSVVFPTGRH LAPRVRIFVD WLAETLGGLR RAD

NT Sequence

ATGGATCATC TGCAAGCAAT GCGCATCTTC GCGCGCGTCG CCCATCTCGG CAGCTTCACG AAGGCGGCCG AGCAGCTCCA GTTGCCGCGC CCGACGGTCA GCAACGCCGT CCAGTATCTG GAAAAACACC TGAAGATCCG GCTGCTGCAA CGCACGACGC GACGCGTCGC GCTGACCGCC GAGGGCGCGA CCTACTACGA ACGCTGCGTG CGGTTGCTGG CCGATCTCGA CGACGCCGAA ACGCTGTTCG AGGATGCCGG CGCGTCGCCG CGCGGCGTGA TCCGCGTCGA CCTGCCCGAA CGGTTCGCGC TGAACCAGGT GATTCCGGCG CTGCCGGATT TCCACGCGCG CTACCCCGAC CTGCGCGTCG CGATCAGCAC GACCGACCGC TTCGTCGATC TCGTCGCGGA CGGCATCGAC TGCGCGGTGC GGGTCGGCGC GCTGTCCGAC ACGTCGCTCG TCGCGCGGCG CGTCGGCGAG ATGGCCCAGA TCAATTGCGC GTCGCCCGCC TATCTCGCGC GCCACGGCAC GCCGCGCTCG CCGGACGATC TGCCGGACCA CGTCGCGGTC GGCTATTTCT CGAGCCGCAC GGGCCGCGAG CTGGACTGGG AATATGCGGA CATGGATTCA GGCGAGATGC ATACGGTGAA GATGCACAGC GTCGTGTCGG TCAACAGTTC GCAAGCGTAT CTCGCGTGCT GCCTCGCGGG CCTCGGGCTG ATCCAGGCGC CGCGCGACGG GCTCGCGCCG CTGCTCGCCG ACGGCTCGCT GGTCGAGGTG CTGCCGGAAT GGCGTGCCGG GCCGCTGCCC GTATCGGTGG TGTTTCCGAC CGGCCGGCAT CTCGCGCCGC GCGTGCGGAT CTTCGTCGAC TGGCTCGCGG AAACGCTCGG CGGCCTGCGC CGGGCGGAC

Details for BuceA.12800.v.A1.GE31877

HARVESTED ON:	3/22/2011
SEQUENCED ON:	3/25/2011
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMDHLQAM RIFARVAHLG SFTKAAEQLQ LPRPTVSNAV QYLEKHLKIR LLQRTTRRVA LTAEGATYYE RCVRLLADLD DAETLFEDAG ASPRGVIRVD LPERFALNQV IPALPDFHAR YPDLRVAIST TDRFVDLVAD GIDCAVRVGA LSDTSLVARR VGEMAQINCA SPAYLARHGT PRSPDDLPDH VAVGYFSSRT GRELDWEYAD MDSGEMHTVK MHSVVSVNSS QAYLACCLAG LGLIQAPRDG LAPLLADGSL VEVLPEWRAG PLPVSVVFPT GRHLAPRVRI FVDWLAETLG GLRRA

Validated NT Sequence

tccgcccggc gcaggccgcc gagcgtttcc gcgagccagt cgacgaagat ccgcacgcgc ggcgcgagat gccggccggt cggaaacacc accgatacgg gcagcggccc ggcacgccat tccggcagca cctcgaccag cgagccgtcg gcgagcagcg gcgcgagccc gtcgcgcggc gcctggatca gcccgaggcc cgcgaggcag cacgcgagat acgcttgcga actgttgacc gacacgacgc tgtgcatctt caccgtatgc atctcgcctg aatccatgtc cgcatattcc cagtccagct cgcggcccgt gcggctcgag aaatagccga ccgcgacgtg gtccggcaga tcgtccggcg agcgcggcgt gccgtggcgc gcgagatagg cgggcgacgc gcaattgatc tgggccatct cgccgacgcg ccgcgcgacg agcgacgtgt cggacagcgc gccgacccgc accgcgcagt cgatgccgtc cgcgacgaga tcgacgaagc ggtcggtcgt gctgatcgcg acgcgcaggt cggggtagcg cgcgtggaaa tccggcagcg ccggaatcac ctggttcagc gcgaaccgtt cgggcaggtc gacgcggatc acgccgcgcg gcgacgcgcc ggcatcctcg aacagcgttt cggcgtcgtc gagatcggcc agcaaccgca cgcagcgttc gtagtaggtc gcgccctcgg cggtcagcgc gacgcgtcgc gtcgtgcgtt gcagcagccg gatcttcagg tgtttttcca gatactggac ggcgttgctg accgtcgggc gcggcaactg gagctgctcg gccgccttcg tgaagctgcc gagatgggcg acgcgcgcga agatgcgcat tgcttgcaga tgatccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDHLQAMRI FARVAHLGSF TKAAEQLQLP RPTVSNAVQY LEKHLKIRLL QRTTRRVALT AEGATYYERC VRLLADLDDA ETLFEDAGAS PRGVIRVDLP ERFALNQVIP ALPDFHARYP DLRVAISTTD RFVDLVADGI DCAVRVGALS DTSLVARRVG EMAQINCASP AYLARHGTPR SPDDLPDHVA VGYFSSRTGR ELDWEYADMD SGEMHTVKMH SVVSVNSSQA YLACCLAGLG LIQAPRDGLA PLLADGSLVE VLPEWRAGPL PVSVVFPTGR HLAPRVRIFV DWLAETLGGL RRAD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatcatctg caagcaatgc gcatcttcgc gcgcgtcgcc catctcggca gcttcacgaa ggcggccgag cagctccagt tgccgcgccc gacggtcagc aacgccgtcc agtatctgga aaaacacctg aagatccggc tgctgcaacg cacgacgcga cgcgtcgcgc tgaccgccga gggcgcgacc tactacgaac gctgcgtgcg gttgctggcc gatctcgacg acgccgaaac gctgttcgag gatgccggcg cgtcgccgcg cggcgtgatc cgcgtcgacc tgcccgaacg gttcgcgctg aaccaggtga ttccggcgct gccggatttc cacgcgcgct accccgacct gcgcgtcgcg atcagcacga ccgaccgctt cgtcgatctc gtcgcggacg gcatcgactg cgcggtgcgg gtcggcgcgc tgtccgacac gtcgctcgtc gcgcggcgcg tcggcgagat ggcccagatc aattgcgcgt cgcccgccta tctcgcgcgc cacggcacgc cgcgctcgcc ggacgatctg ccggaccacg tcgcggtcgg ctatttctcg agccgcacgg gccgcgagct ggactgggaa tatgcggaca tggattcagg cgagatgcat acggtgaaga tgcacagcgt cgtgtcggtc aacagttcgc aagcgtatct cgcgtgctgc ctcgcgggcc tcgggctgat ccaggcgccg cgcgacgggc tcgcgccgct gctcgccgac ggctcgctgg tcgaggtgct gccggaatgg cgtgccgggc cgctgcccgt atcggtggtg tttccgaccg gccggcatct cgcgccgcgc gtgcggatct tcgtcgactg gctcgcggaa acgctcggcg gcctgcgccg ggcggactaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for BuceA.12800.v.A1.PW31790

PURIFICATION DATe:	5/13/2011
CONCENTRATION:	29.86mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATCATCTG CAAGCAATGC GCATCTTCGC GCGCGTCGCC CATCTCGGCA GCTTCACGAA GGCGGCCGAG CAGCTCCAGT TGCCGCGCCC GACGGTCAGC AACGCCGTCC AGTATCTGGA AAAACACCTG AAGATCCGGC TGCTGCAACG CACGACGCGA CGCGTCGCGC TGACCGCCGA GGGCGCGACC TACTACGAAC GCTGCGTGCG GTTGCTGGCC GATCTCGACG ACGCCGAAAC GCTGTTCGAG GATGCCGGCG CGTCGCCGCG CGGCGTGATC CGCGTCGACC TGCCCGAACG GTTCGCGCTG AACCAGGTGA TTCCGGCGCT GCCGGATTTC CACGCGCGCT ACCCCGACCT GCGCGTCGCG ATCAGCACGA CCGACCGCTT CGTCGATCTC GTCGCGGACG GCATCGACTG CGCGGTGCGG GTCGGCGCGC TGTCCGACAC GTCGCTCGTC GCGCGGCGCG TCGGCGAGAT GGCCCAGATC AATTGCGCGT CGCCCGCCTA TCTCGCGCGC CACGGCACGC CGCGCTCGCC GGACGATCTG CCGGACCACG TCGCGGTCGG CTATTTCTCG AGCCGCACGG GCCGCGAGCT GGACTGGGAA TATGCGGACA TGGATTCAGG CGAGATGCAT ACGGTGAAGA TGCACAGCGT CGTGTCGGTC AACAGTTCGC AAGCGTATCT CGCGTGCTGC CTCGCGGGCC TCGGGCTGAT CCAGGCGCCG CGCGACGGGC TCGCGCCGCT GCTCGCCGAC GGCTCGCTGG TCGAGGTGCT GCCGGAATGG CGTGCCGGGC CGCTGCCCGT ATCGGTGGTG TTTCCGACCG GCCGGCATCT CGCGCCGCGC GTGCGGATCT TCGTCGACTG GCTCGCGGAA ACGCTCGGCG GCCTGCGCCG GGCGGACTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA