BuceA.12800.u

LysR family regulatory protein

CENTER ID: BuceA.12800.u
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12800.u.A1.GE31876 Full length( BuceA.12800.u ) 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.5544
RefSeq: YP_002234371.1
UniProt: B4ELR8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLDSIRIFL RVVERGSFTA ASVELDMPLS RVSRKVKHLE DDLGVQLLYR TTRRVSVTEA GRDYYDRCLR AEDILLDADR QVRTLRTDPE GTLRVLVPYA IGLFQLEPAL AEFRRRYPLV QLVLIYDNSP LDLVEHGFDV ALRAGLLNDS GYVARSLGWS HAKLAASPAY LDRTGRPRTP DDLAQHDILF VGRDAPGLTL RLSNTDGERA DVPVQPVLIS NESVTVLRQA TSGGGIALIS THFTARRLAR NELEIVLPDW HRTNDVELHA LYPRRATLDS KVRAFVEFLT ELFATWRTAP
NT Sequence
ATGGACCTCG ATAGCATCCG AATCTTCTTG CGCGTAGTGG AACGCGGCAG CTTCACCGCC GCGTCCGTGG AACTCGACAT GCCGCTCAGT CGCGTGAGCC GCAAGGTCAA GCACCTCGAG GACGATCTCG GCGTGCAACT GCTGTACCGG ACCACACGCC GCGTGTCGGT CACCGAAGCC GGGCGCGATT ACTACGATCG CTGCCTGCGC GCCGAGGACA TCCTGCTCGA CGCGGACCGC CAGGTCCGCA CGCTTCGCAC CGACCCCGAA GGCACGCTGC GCGTGCTGGT GCCCTATGCG ATCGGCCTGT TCCAGCTCGA ACCGGCGCTC GCCGAATTCC GCCGGCGCTA CCCGCTGGTG CAGCTGGTGC TGATCTACGA CAACAGTCCG CTCGACCTCG TCGAGCATGG CTTCGACGTC GCGCTGCGCG CGGGTCTGCT GAACGATTCG GGCTACGTCG CGCGCTCGCT CGGCTGGTCG CATGCGAAGC TCGCCGCGAG CCCGGCCTAC CTGGATCGCA CCGGCCGGCC GCGCACGCCG GACGATCTCG CGCAACACGA CATCCTGTTC GTCGGCCGCG ATGCGCCGGG CCTGACGCTG CGGCTCAGCA ACACCGACGG CGAGCGGGCC GACGTCCCGG TGCAGCCCGT GCTGATCTCG AACGAATCGG TCACGGTATT GCGCCAGGCC ACGAGCGGCG GCGGCATTGC GCTGATCTCC ACGCATTTCA CCGCGCGACG GCTCGCGAGG AACGAGCTCG AGATCGTGCT GCCCGACTGG CACCGTACGA ACGACGTCGA GCTTCACGCG CTCTATCCGC GCCGCGCGAC GCTCGACAGC AAGGTGCGGG CGTTCGTCGA GTTCCTCACC GAACTGTTCG CCACCTGGCG AACCGCGCCG
Details for BuceA.12800.u.A1.GE31876
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDLDSIRIF LRVVERGSFT AASVELDMPL SRVSRKVKHL EDDLGVQLLY RTTRRVSVTE AGRDYYDRCL RAEDILLDAD RQVRTLRTDP EGTLRVLVPY AIGLFQLEPA LAEFRRRYPL VQLVLIYDNS PLDLVEHGFD VALRAGLLND SGYVARSLGW SHAKLAASPA YLDRTGRPRT PDDLAQHDIL FVGRDAPGLT LRLSNTDGER ADVPVQPVLI SNESVTVLRQ ATSGGGIALI STHFTARRLA RXXLEIVLPD WHRTNDVELH ALYPRRATLD SKXRAFVEFL TELXATWXXA X
Validated NT Sequence
gnnnnnnnnn nnnnnatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggacc tcgatagcat ccgaatcttc ttgcgcgtag tggaacgcgg cagcttcacc gccgcgtccg tggaactcga catgccgctc agtcgcgtga gccgcaaggt caagcacctc gaggacgatc tcggcgtgca actgctgtac cggaccacac gccgcgtgtc ggtcaccgaa gccgggcgcg attactacga tcgctgcctg cgcgccgagg acatcctgct cgacgcggac cgccaggtcc gcacgcttcg caccgacccc gaaggcacgc tgcgcgtgct ggtgccctat gcgatcggcc tgttccagct cgaaccggcg ctcgccgaat tccgccggcg ctacccgctg gtgcagctgg tgctgatcta cgacaacagt ccgctcgacc tcgtcgagca tggcttcgac gtcgcgctgc gcgcgggtct gctgaacgat tcgggctacg tcgcgcgctc gctcggctgg tcgcatgcga agctcgccgc gagcccggcc tacctggatc gcaccggccg gccgcgcacg ccggacgatc tcgcgcaaca cgacatcctg ttcgtcggcc gcgatgcgcc gggcctgacg ctgcggctca gcaacaccga cggcgagcgg gccgacgtcc cggtgcagcc cgtgctgatc tcgaacgaat cggtcacggt attgcgccag gccacgagcg gcggcggcat tgcgctgatc tccacgcatt tcaccgcgcg acggctcgcg aggnnnnnnc tcgagatcgt gctgcccgac tggcaccgta cgaacgacgt cgagcttcac gcgctctatc cgcgccgcgc gacgctcgac agcaaggngc gggcgttcgt cgagttcctc accgaactgn tcgccacctg gngaancgcg ncgtaatnaa acagcacgaa cnagttctgc agccnagctn cncnnngntn cnnctgctaa caaannccga annnagcngn actgctgcac gc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLDSIRIF LRVVERGSFT AASVELDMPL SRVSRKVKHL EDDLGVQLLY RTTRRVSVTE AGRDYYDRCL RAEDILLDAD RQVRTLRTDP EGTLRVLVPY AIGLFQLEPA LAEFRRRYPL VQLVLIYDNS PLDLVEHGFD VALRAGLLND SGYVARSLGW SHAKLAASPA YLDRTGRPRT PDDLAQHDIL FVGRDAPGLT LRLSNTDGER ADVPVQPVLI SNESVTVLRQ ATSGGGIALI STHFTARRLA RNELEIVLPD WHRTNDVELH ALYPRRATLD SKVRAFVEFL TELFATWRTA P
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacctcgat agcatccgaa tcttcttgcg cgtagtggaa cgcggcagct tcaccgccgc gtccgtggaa ctcgacatgc cgctcagtcg cgtgagccgc aaggtcaagc acctcgagga cgatctcggc gtgcaactgc tgtaccggac cacacgccgc gtgtcggtca ccgaagccgg gcgcgattac tacgatcgct gcctgcgcgc cgaggacatc ctgctcgacg cggaccgcca ggtccgcacg cttcgcaccg accccgaagg cacgctgcgc gtgctggtgc cctatgcgat cggcctgttc cagctcgaac cggcgctcgc cgaattccgc cggcgctacc cgctggtgca gctggtgctg atctacgaca acagtccgct cgacctcgtc gagcatggct tcgacgtcgc gctgcgcgcg ggtctgctga acgattcggg ctacgtcgcg cgctcgctcg gctggtcgca tgcgaagctc gccgcgagcc cggcctacct ggatcgcacc ggccggccgc gcacgccgga cgatctcgcg caacacgaca tcctgttcgt cggccgcgat gcgccgggcc tgacgctgcg gctcagcaac accgacggcg agcgggccga cgtcccggtg cagcccgtgc tgatctcgaa cgaatcggtc acggtattgc gccaggccac gagcggcggc ggcattgcgc tgatctccac gcatttcacc gcgcgacggc tcgcgaggaa cgagctcgag atcgtgctgc ccgactggca ccgtacgaac gacgtcgagc ttcacgcgct ctatccgcgc cgcgcgacgc tcgacagcaa ggtgcgggcg ttcgtcgagt tcctcaccga actgttcgcc acctggcgaa ccgcgccgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a