BuceA.12800.t

LysR family regulatory protein

CENTER ID: BuceA.12800.t
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12800.t.A1.GE31875 Full length( BuceA.12800.t ) 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12800.t.A1.PW31788 Full length( BuceA.12800.t ) 1 300

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4384
RefSeq: YP_002233307.1
UniProt: B4ELN4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRLQTMQIY VQIVERGNFT QAADALQLHR PAVTKAVQQL EQELGVRLLN RSTRRVSVTA EGEAFHARCV QLLGSIDDAF ASFPKQRAVP KGRLRIDVAL ALAKTVVIPA LPDFQQRYPQ IELVLGASDQ PADLIGEGID CVVRVGTLKD SSMVARTVGA VPMATCASPA YIARHGLPRT LDDLRMHRAI NFFTGRNRRV LEWTFRPHGD TVAVKLASSL LTDNSEALLS CGLAGLGIVH ALRPALQPSI DSGQLVELLP GVPAVPKPVS VLYPNRAHLP DKVRAFIDWL TELFARRYPA
NT Sequence
ATGGACCGCC TGCAAACGAT GCAGATCTAC GTACAGATCG TCGAACGCGG CAACTTCACG CAGGCCGCCG ACGCGCTGCA GCTGCATCGG CCGGCCGTCA CCAAAGCCGT GCAGCAGCTC GAACAGGAGC TCGGCGTGCG GCTGCTGAAC CGCAGCACGC GCCGCGTCAG CGTGACGGCC GAAGGCGAGG CATTCCATGC GCGCTGCGTG CAGTTGCTCG GCAGCATCGA CGATGCGTTC GCGTCGTTCC CGAAGCAACG CGCGGTGCCG AAGGGCCGGC TGCGGATCGA CGTTGCCCTC GCGCTTGCGA AGACGGTCGT GATTCCGGCG CTGCCCGACT TCCAGCAGCG CTATCCGCAG ATCGAGCTCG TGCTCGGCGC GAGCGACCAG CCGGCCGACC TGATCGGCGA AGGCATCGAC TGCGTGGTGC GGGTCGGGAC GCTGAAGGAT TCGAGCATGG TCGCGCGCAC CGTCGGCGCG GTGCCGATGG CCACATGCGC GTCGCCGGCC TATATCGCGC GTCACGGCTT GCCGCGTACG CTCGACGATC TGCGGATGCA TCGCGCGATC AACTTCTTCA CCGGCCGCAA CCGCCGCGTG CTCGAATGGA CGTTCCGCCC GCACGGCGAC ACCGTCGCGG TGAAGCTCGC GAGCAGCCTG CTCACCGACA ACTCGGAAGC ATTGCTGTCG TGCGGGCTCG CCGGTCTCGG CATCGTGCAC GCGTTGCGGC CCGCGCTGCA GCCGAGCATC GACTCGGGCC AGCTGGTCGA GCTGCTGCCC GGCGTGCCGG CCGTGCCGAA GCCGGTGTCG GTGCTGTACC CGAACCGCGC GCATCTGCCC GACAAGGTGC GCGCGTTCAT CGACTGGCTG ACCGAGCTGT TCGCGCGACG TTATCCGGCG
Details for BuceA.12800.t.A1.GE31875
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 18
PERCENT COVERAGE: 78
Validated AA Sequence
MRAVRVQHRH RLRHGRHAGQ QLDQLARVDA RLQRGPQRVH DAETGEPARQ QCFRVVGEQA ARELHRDGVA VRAERPFEHA AVAAGEEVDR AMHPQIVERT RQAVTRDIGR RRACGHRHRA DGARDHARIL QRPDPHHAVD AFADQVGRLV ARAEHELDLR IALLEVGQRR NHDRLRKREG NVDPQPALRH RALLRERRER IVDAAEQLHA ARMECLAFGR HADAARAAVQ QPHAELLFEL LHGFGDGRPM QL
Validated NT Sequence
gaacagctcg gtcagccagt cgatgaacgc gcgcaccttg tcgggcagat gcgcgcggtt cgggtacagc accgacaccg gcttcggcac ggccggcacg ccgggcagca gctcgaccag ctggcccgag tcgatgctcg gctgcagcgc gggccgcaac gcgtgcacga tgccgagacc ggcgagcccg cacgacagca atgcttccga gttgtcggtg agcaggctgc tcgcgagctt caccgcgacg gtgtcgccgt gcgggcggaa cgtccattcg agcacgcggc ggttgcggcc ggtgaagaag ttgatcgcgc gatgcatccg cagatcgtcg agcgtacgcg gcaagccgtg acgcgcgata taggccggcg acgcgcatgt ggccatcggc accgcgccga cggtgcgcgc gaccatgctc gaatccttca gcgtcccgac ccgcaccacg cagtcgatgc cttcgccgat caggtcggcc ggctggtcgc tcgcgccgag cacgagctcg atctgcggat agcgctgctg gaagtcgggc agcgccggaa tcacgaccgt cttcgcaagc gcgagggcaa cgtcgatccg cagccggccc ttcggcaccg cgcgttgctt cgggaacgac gcgaacgcat cgtcgatgct gccgagcaac tgcacgcagc gcgcatggaa tgcctcgcct tcggccgtca cgctgacgcg gcgcgtgctg cggttcagca gccgcacgcc gagctcctgt tcgagctgct gcacggcttt ggtgacggcc ggccgatgca gctgcag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRLQTMQI YVQIVERGNF TQAADALQLH RPAVTKAVQQ LEQELGVRLL NRSTRRVSVT AEGEAFHARC VQLLGSIDDA FASFPKQRAV PKGRLRIDVA LALAKTVVIP ALPDFQQRYP QIELVLGASD QPADLIGEGI DCVVRVGTLK DSSMVARTVG AVPMATCASP AYIARHGLPR TLDDLRMHRA INFFTGRNRR VLEWTFRPHG DTVAVKLASS LLTDNSEALL SCGLAGLGIV HALRPALQPS IDSGQLVELL PGVPAVPKPV SVLYPNRAHL PDKVRAFIDW LTELFARRYP A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccgcctg caaacgatgc agatctacgt acagatcgtc gaacgcggca acttcacgca ggccgccgac gcgctgcagc tgcatcggcc ggccgtcacc aaagccgtgc agcagctcga acaggagctc ggcgtgcggc tgctgaaccg cagcacgcgc cgcgtcagcg tgacggccga aggcgaggca ttccatgcgc gctgcgtgca gttgctcggc agcatcgacg atgcgttcgc gtcgttcccg aagcaacgcg cggtgccgaa gggccggctg cggatcgacg ttgccctcgc gcttgcgaag acggtcgtga ttccggcgct gcccgacttc cagcagcgct atccgcagat cgagctcgtg ctcggcgcga gcgaccagcc ggccgacctg atcggcgaag gcatcgactg cgtggtgcgg gtcgggacgc tgaaggattc gagcatggtc gcgcgcaccg tcggcgcggt gccgatggcc acatgcgcgt cgccggccta tatcgcgcgt cacggcttgc cgcgtacgct cgacgatctg cggatgcatc gcgcgatcaa cttcttcacc ggccgcaacc gccgcgtgct cgaatggacg ttccgcccgc acggcgacac cgtcgcggtg aagctcgcga gcagcctgct caccgacaac tcggaagcat tgctgtcgtg cgggctcgcc ggtctcggca tcgtgcacgc gttgcggccc gcgctgcagc cgagcatcga ctcgggccag ctggtcgagc tgctgcccgg cgtgccggcc gtgccgaagc cggtgtcggt gctgtacccg aaccgcgcgc atctgcccga caaggtgcgc gcgttcatcg actggctgac cgagctgttc gcgcgacgtt atccggcgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BuceA.12800.t.A1.PW31788
PURIFICATION DATe: 7/5/2011
CONCENTRATION: 3.81mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 18
PERCENT COVERAGE: 78
Protocol Notes
notes unavailable
Validated AA Sequence
MRAVRVQHRH RLRHGRHAGQ QLDQLARVDA RLQRGPQRVH DAETGEPARQ QCFRVVGEQA ARELHRDGVA VRAERPFEHA AVAAGEEVDR AMHPQIVERT RQAVTRDIGR RRACGHRHRA DGARDHARIL QRPDPHHAVD AFADQVGRLV ARAEHELDLR IALLEVGQRR NHDRLRKREG NVDPQPALRH RALLRERRER IVDAAEQLHA ARMECLAFGR HADAARAAVQ QPHAELLFEL LHGFGDGRPM QL
Validated NT Sequence
gaacagctcg gtcagccagt cgatgaacgc gcgcaccttg tcgggcagat gcgcgcggtt cgggtacagc accgacaccg gcttcggcac ggccggcacg ccgggcagca gctcgaccag ctggcccgag tcgatgctcg gctgcagcgc gggccgcaac gcgtgcacga tgccgagacc ggcgagcccg cacgacagca atgcttccga gttgtcggtg agcaggctgc tcgcgagctt caccgcgacg gtgtcgccgt gcgggcggaa cgtccattcg agcacgcggc ggttgcggcc ggtgaagaag ttgatcgcgc gatgcatccg cagatcgtcg agcgtacgcg gcaagccgtg acgcgcgata taggccggcg acgcgcatgt ggccatcggc accgcgccga cggtgcgcgc gaccatgctc gaatccttca gcgtcccgac ccgcaccacg cagtcgatgc cttcgccgat caggtcggcc ggctggtcgc tcgcgccgag cacgagctcg atctgcggat agcgctgctg gaagtcgggc agcgccggaa tcacgaccgt cttcgcaagc gcgagggcaa cgtcgatccg cagccggccc ttcggcaccg cgcgttgctt cgggaacgac gcgaacgcat cgtcgatgct gccgagcaac tgcacgcagc gcgcatggaa tgcctcgcct tcggccgtca cgctgacgcg gcgcgtgctg cggttcagca gccgcacgcc gagctcctgt tcgagctgct gcacggcttt ggtgacggcc ggccgatgca gctgcag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRLQTMQI YVQIVERGNF TQAADALQLH RPAVTKAVQQ LEQELGVRLL NRSTRRVSVT AEGEAFHARC VQLLGSIDDA FASFPKQRAV PKGRLRIDVA LALAKTVVIP ALPDFQQRYP QIELVLGASD QPADLIGEGI DCVVRVGTLK DSSMVARTVG AVPMATCASP AYIARHGLPR TLDDLRMHRA INFFTGRNRR VLEWTFRPHG DTVAVKLASS LLTDNSEALL SCGLAGLGIV HALRPALQPS IDSGQLVELL PGVPAVPKPV SVLYPNRAHL PDKVRAFIDW LTELFARRYP A
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACCGCCTG CAAACGATGC AGATCTACGT ACAGATCGTC GAACGCGGCA ACTTCACGCA GGCCGCCGAC GCGCTGCAGC TGCATCGGCC GGCCGTCACC AAAGCCGTGC AGCAGCTCGA ACAGGAGCTC GGCGTGCGGC TGCTGAACCG CAGCACGCGC CGCGTCAGCG TGACGGCCGA AGGCGAGGCA TTCCATGCGC GCTGCGTGCA GTTGCTCGGC AGCATCGACG ATGCGTTCGC GTCGTTCCCG AAGCAACGCG CGGTGCCGAA GGGCCGGCTG CGGATCGACG TTGCCCTCGC GCTTGCGAAG ACGGTCGTGA TTCCGGCGCT GCCCGACTTC CAGCAGCGCT ATCCGCAGAT CGAGCTCGTG CTCGGCGCGA GCGACCAGCC GGCCGACCTG ATCGGCGAAG GCATCGACTG CGTGGTGCGG GTCGGGACGC TGAAGGATTC GAGCATGGTC GCGCGCACCG TCGGCGCGGT GCCGATGGCC ACATGCGCGT CGCCGGCCTA TATCGCGCGT CACGGCTTGC CGCGTACGCT CGACGATCTG CGGATGCATC GCGCGATCAA CTTCTTCACC GGCCGCAACC GCCGCGTGCT CGAATGGACG TTCCGCCCGC ACGGCGACAC CGTCGCGGTG AAGCTCGCGA GCAGCCTGCT CACCGACAAC TCGGAAGCAT TGCTGTCGTG CGGGCTCGCC GGTCTCGGCA TCGTGCACGC GTTGCGGCCC GCGCTGCAGC CGAGCATCGA CTCGGGCCAG CTGGTCGAGC TGCTGCCCGG CGTGCCGGCC GTGCCGAAGC CGGTGTCGGT GCTGTACCCG AACCGCGCGC ATCTGCCCGA CAAGGTGCGC GCGTTCATCG ACTGGCTGAC CGAGCTGTTC GCGCGACGTT ATCCGGCGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A