BuceA.12800.r

LysR family regulatory protein

CENTER ID: BuceA.12800.r
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12800.r.A1.GE31873 Full length( BuceA.12800.r ) 1 309
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4257
RefSeq: YP_002233190.1
UniProt: B4EKL1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDERLRGVAE FVDVVESGSF AAAALRLGMT RSAVAKIVAR LELRLGARLL QRTTRQLGLT DEGFVYYEQC RRLLAELGET EAALDAGRRE PAGRVRISVP VLFGRQCVAP VVRRLVERHP RLEIDMSFSD RVADLVNDGF DLAVRIGELA DTSALVGRKL GVQRMGICAS PDYLARHGRP SGLEALASHV GIAYSRGGQP TPWRIIGADG AVHDHRAAGR LRFDDLQAIA DAAAAGAGLA WLPCWLMAPY VRDGRLALVM DSDSVSGAEV FAVRPAARHV PSKVRVVVDA LVDEIPHMLA SDDASGERT
NT Sequence
ATGGACGAAC GGCTCAGAGG CGTCGCCGAA TTTGTCGACG TGGTGGAATC GGGTAGCTTC GCGGCGGCGG CGCTGCGCCT CGGCATGACG CGCTCGGCCG TCGCGAAGAT CGTCGCGCGG CTCGAACTCC GGCTCGGCGC GCGGCTGCTG CAGCGCACGA CGCGCCAGCT CGGCCTGACC GACGAAGGGT TCGTCTATTA CGAGCAGTGC CGGCGGCTGC TGGCCGAACT GGGCGAGACG GAAGCCGCGC TCGACGCGGG CCGCCGCGAG CCGGCCGGGC GCGTGCGCAT CAGCGTGCCC GTGCTGTTCG GGCGGCAGTG CGTGGCGCCC GTCGTGCGGC GGCTCGTCGA GCGCCATCCG CGGCTCGAAA TCGACATGTC GTTCAGCGAT CGCGTGGCCG ACCTCGTCAA CGACGGTTTC GACCTGGCCG TGCGCATCGG CGAGCTGGCC GATACGAGCG CGCTGGTCGG CCGCAAGCTG GGCGTGCAAC GCATGGGCAT CTGCGCATCG CCCGACTATC TCGCGCGTCA TGGCCGGCCG TCGGGGCTCG AGGCACTCGC GTCGCACGTC GGCATCGCGT ATTCGCGCGG CGGACAGCCG ACACCGTGGC GGATCATCGG CGCGGATGGC GCGGTGCACG ACCATCGCGC GGCCGGCCGC CTGCGGTTCG ACGACCTGCA GGCGATCGCC GACGCGGCCG CCGCCGGCGC GGGCCTCGCG TGGCTGCCGT GCTGGCTGAT GGCGCCGTAC GTGCGCGACG GCCGCCTCGC GCTCGTGATG GACAGCGACA GCGTGTCGGG CGCCGAAGTG TTCGCGGTGC GGCCGGCGGC GCGCCACGTG CCGTCGAAGG TGCGGGTCGT CGTCGATGCG CTGGTCGACG AGATTCCGCA CATGCTGGCG TCCGACGACG CGAGCGGCGA GCGTACG
Details for BuceA.12800.r.A1.GE31873
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 43
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMDERLRG VAEFVDVVES GSFAAAALRL GMTRSAVAKI VARLELRLGA RLLQRTTRPA RPDRRRVRLL RAVPAAAGRT GRDGSRARRG PPRAGRARAH QRARAVRAAV RGARRAAARR APSAARNRHV VQRSRGRPRQ RRFRPGRAHR RAGRYERAGR PQAGRATHGH LRIARLSRAS WPAVGARGTR VARRHRVFAR RTADTVADHR RGWRGARPSR GRPPAVRRPA GDRRRGRRRR GPRVAAVLAD GAVRARRPPR ARDGQRQRVG RRSVRGAAGG APRAVEGAGR RRCAGRRDSA HAGVRRRE
Validated NT Sequence
cgctcgcgtc gtcggacgcc agcatgtgcg gaatctcgtc gaccagcgca tcgacgacga cccgcacctt cgacggcacg tggcgcgccg ccggccgcac cgcgaacact tcggcgcccg acacgctgtc gctgtccatc acgagcgcga ggcggccgtc gcgcacgtac ggcgccatca gccagcacgg cagccacgcg aggcccgcgc cggcggcggc cgcgtcggcg atcgcctgca ggtcgtcgaa ccgcaggcgg ccggccgcgc gatggtcgtg caccgcgcca tccgcgccga tgatccgcca cggtgtcggc tgtccgccgc gcgaatacgc gatgccgacg tgcgacgcga gtgcctcgag ccccgacggc cggccatgac gcgcgagata gtcgggcgat gcgcagatgc ccatgcgttg cacgcccagc ttgcggccga ccagcgcgct cgtatcggcc agctcgccga tgcgcacggc caggtcgaaa ccgtcgttga cgaggtcggc cacgcgatcg ctgaacgaca tgtcgatttc gagccgcgga tggcgctcga cgagccgccg cacgacgggc gccacgcact gccgcccgaa cagcacgggc acgctgatgc gcacgcgccc ggccggctcg cggcggcccg cgtcgagcgc ggcttccgtc tcgcccagtt cggccagcag ccgccggcac tgctcgtaat agacgaaccc ttcgtcggtc aggccgagct gggcgcgtcg tgcgctgcag cagccgcgcg ccgagccgga gttcgagccg cgcgacgatc ttcgcgacgg ccgagcgcgt catgccgagg cgcagcgccg ccgccgcgaa gctacccgat tccaccacgt cgacaaattc ggcgacgcct ctgagccgtt cgtccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDERLRGVA EFVDVVESGS FAAAALRLGM TRSAVAKIVA RLELRLGARL LQRTTRQLGL TDEGFVYYEQ CRRLLAELGE TEAALDAGRR EPAGRVRISV PVLFGRQCVA PVVRRLVERH PRLEIDMSFS DRVADLVNDG FDLAVRIGEL ADTSALVGRK LGVQRMGICA SPDYLARHGR PSGLEALASH VGIAYSRGGQ PTPWRIIGAD GAVHDHRAAG RLRFDDLQAI ADAAAAGAGL AWLPCWLMAP YVRDGRLALV MDSDSVSGAE VFAVRPAARH VPSKVRVVVD ALVDEIPHML ASDDASGERT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgaacgg ctcagaggcg tcgccgaatt tgtcgacgtg gtggaatcgg gtagcttcgc ggcggcggcg ctgcgcctcg gcatgacgcg ctcggccgtc gcgaagatcg tcgcgcggct cgaactccgg ctcggcgcgc ggctgctgca gcgcacgacg cgccagctcg gcctgaccga cgaagggttc gtctattacg agcagtgccg gcggctgctg gccgaactgg gcgagacgga agccgcgctc gacgcgggcc gccgcgagcc ggccgggcgc gtgcgcatca gcgtgcccgt gctgttcggg cggcagtgcg tggcgcccgt cgtgcggcgg ctcgtcgagc gccatccgcg gctcgaaatc gacatgtcgt tcagcgatcg cgtggccgac ctcgtcaacg acggtttcga cctggccgtg cgcatcggcg agctggccga tacgagcgcg ctggtcggcc gcaagctggg cgtgcaacgc atgggcatct gcgcatcgcc cgactatctc gcgcgtcatg gccggccgtc ggggctcgag gcactcgcgt cgcacgtcgg catcgcgtat tcgcgcggcg gacagccgac accgtggcgg atcatcggcg cggatggcgc ggtgcacgac catcgcgcgg ccggccgcct gcggttcgac gacctgcagg cgatcgccga cgcggccgcc gccggcgcgg gcctcgcgtg gctgccgtgc tggctgatgg cgccgtacgt gcgcgacggc cgcctcgcgc tcgtgatgga cagcgacagc gtgtcgggcg ccgaagtgtt cgcggtgcgg ccggcggcgc gccacgtgcc gtcgaaggtg cgggtcgtcg tcgatgcgct ggtcgacgag attccgcaca tgctggcgtc cgacgacgcg agcggcgagc gtacgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa