SSGCID: Target Page

BuceA.12800.q

LysR family regulatory protein

CENTER ID:	BuceA.12800.q
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.12800.q.A1.GE31872	Full length( BuceA.12800.q )		1	315

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.5201
RefSeq:	YP_002234068.1
UniProt:	B4EJ08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTLQNMRVF SRVVETGSFT AAAQSLNSTT GAMSRAVSEL EARLRTRLMN RSTRRLALTS AGESYLRRCR QILADVDRAE EEASCAHERP AGVLRMHSFA SVGHHYVMPA LTRYHTQYPD VSIELSLSQR MPDLFDGTSD MAVVTASSLP DSELVSHLLG STFSILCASP DYVKRHGVPA RPQDLAAHAC LTLCTPAFPT HEWVLEGPEG VEQIHVAGPV QTNTAESLAL AIRDGIGIGM LPLYSAIDAL RDGTLVRVLP AHILQKMNVY ALYPSRRFVD AKVRTWVEML RAQVPGMIAR DVEMLNAIDR EPQAA

NT Sequence

ATGGATACCC TACAAAACAT GCGGGTGTTT TCGCGCGTCG TCGAGACGGG CAGCTTCACC GCCGCCGCGC AATCGCTGAA TTCGACGACC GGCGCGATGT CGCGCGCGGT GTCGGAGCTG GAGGCGCGCC TGCGCACCCG TCTGATGAAT CGCTCGACCC GCCGTCTCGC GCTGACGTCG GCCGGCGAAA GCTATCTGCG GCGCTGCCGC CAGATCCTCG CCGACGTCGA CCGCGCGGAA GAAGAAGCGA GCTGCGCGCA CGAGCGCCCG GCCGGCGTGC TGCGCATGCA CAGCTTCGCG AGCGTCGGTC ACCACTACGT GATGCCCGCG CTGACGCGCT ATCACACGCA GTACCCGGAC GTGTCGATCG AGCTGTCGCT GTCGCAGCGC ATGCCCGACC TGTTCGACGG CACGAGCGAC ATGGCCGTCG TCACCGCGTC GTCGCTGCCC GATTCGGAAC TCGTGTCGCA CCTGCTCGGG TCGACCTTCA GCATTCTGTG TGCGTCACCC GACTACGTGA AACGGCACGG CGTGCCGGCC CGCCCGCAGG ATCTCGCCGC GCACGCGTGC CTGACGCTGT GCACGCCGGC GTTTCCGACG CACGAATGGG TGCTCGAAGG GCCCGAAGGC GTCGAGCAGA TCCACGTCGC CGGGCCGGTG CAGACCAATA CCGCCGAATC GCTCGCGCTC GCGATCCGCG ACGGCATCGG GATCGGCATG CTGCCGCTGT ATTCGGCGAT CGACGCGCTG CGCGACGGCA CGCTGGTCCG TGTGCTGCCC GCGCATATCC TGCAGAAGAT GAACGTGTAT GCGCTGTACC CGTCGCGCCG TTTCGTCGAT GCGAAGGTGC GGACCTGGGT CGAGATGTTG CGTGCGCAAG TGCCGGGAAT GATCGCGCGC GACGTCGAGA TGCTGAACGC GATCGACCGC GAACCGCAGG CCGCC

Details for BuceA.12800.q.A1.GE31872

HARVESTED ON:	3/22/2011
SEQUENCED ON:	3/25/2011
EXPECTED MW:	36kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	46
PERCENT COVERAGE:	94

Validated AA Sequence

MDTLQNMRVF SRVVETGSFT AAAQSLNSTT GAMSRAVSEL EARLRTRLMN RSTRRLALTS AGESYLRRCR QILADVDRAE EEASCAHERP GRRAAHAQLR ERRSPLRDAR ADALSHAVPG RVDRAVAVAA HARPVRRHER HGRRHRVVAA RFGTRVAPAR VDLQHSVCVT RLRETARRAG PPAGSRRARV PDAVHAGVSD ARMGARRARR RRADPRRRAG ADQYRRIARA RDPRRHRDRH AAAVFGDRRA ARRHAGPCAA RAYPAEDERV CAVPVAPFRR CEGADLGRDV ACASAGNDRA RRRDAERDRP RTAGRLI

Validated NT Sequence

ttattaggcg gcctgcggtt cgcggtcgat cgcgttcagc atctcgacgt cgcgcgcgat cattcccggc acttgcgcac gcaacatctc gacccaggtc cgcaccttcg catcgacgaa acggcgcgac gggtacagcg catacacgtt catcttctgc aggatatgcg cgggcagcac acggaccagc gtgccgtcgc gcagcgcgtc gatcgccgaa tacagcggca gcatgccgat cccgatgccg tcgcggatcg cgagcgcgag cgattcggcg gtattggtct gcaccggccc ggcgacgtgg atctgctcga cgccttcggg cccttcgagc acccattcgt gcgtcggaaa cgccggcgtg cacagcgtca ggcacgcgtg cgcggcgaga tcctgcgggc gggccggcac gccgtgccgt ttcacgtagt cgggtgacgc acacagaatg ctgaaggtcg acccgagcag gtgcgacacg agttccgaat cgggcagcga cgacgcggtg acgacggcca tgtcgctcgt gccgtcgaac aggtcgggca tgcgctgcga cagcgacagc tcgatcgaca cgtccgggta ctgcgtgtga tagcgcgtca gcgcgggcat cacgtagtgg tgaccgacgc tcgcgaagct gtgcatgcgc agcacgccgg ccggggcgct cgtgcgcgca gctcgcttct tcttccgcgc ggtcgacgtc ggcgaggatc tggcggcagc gccgcagata gctttcgccg gccgacgtca gcgcgagacg gcgggtcgag cgattcatca gacgggtgcg caggcgcgcc tccagctccg acaccgcgcg cgacatcgcg ccggtcgtcg aattcagcga ttgcgcggcg gcggtgaagc tgcccgtctc gacgacgcgc gaaaacaccc gcatgttttg tagggtatcc atcgaaccag gaccctgggt ctgagcttcc agggtacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDTLQNMRV FSRVVETGSF TAAAQSLNST TGAMSRAVSE LEARLRTRLM NRSTRRLALT SAGESYLRRC RQILADVDRA EEEASCAHER PAGVLRMHSF ASVGHHYVMP ALTRYHTQYP DVSIELSLSQ RMPDLFDGTS DMAVVTASSL PDSELVSHLL GSTFSILCAS PDYVKRHGVP ARPQDLAAHA CLTLCTPAFP THEWVLEGPE GVEQIHVAGP VQTNTAESLA LAIRDGIGIG MLPLYSAIDA LRDGTLVRVL PAHILQKMNV YALYPSRRFV DAKVRTWVEM LRAQVPGMIA RDVEMLNAID REPQAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatacccta caaaacatgc gggtgttttc gcgcgtcgtc gagacgggca gcttcaccgc cgccgcgcaa tcgctgaatt cgacgaccgg cgcgatgtcg cgcgcggtgt cggagctgga ggcgcgcctg cgcacccgtc tgatgaatcg ctcgacccgc cgtctcgcgc tgacgtcggc cggcgaaagc tatctgcggc gctgccgcca gatcctcgcc gacgtcgacc gcgcggaaga agaagcgagc tgcgcgcacg agcgcccggc cggcgtgctg cgcatgcaca gcttcgcgag cgtcggtcac cactacgtga tgcccgcgct gacgcgctat cacacgcagt acccggacgt gtcgatcgag ctgtcgctgt cgcagcgcat gcccgacctg ttcgacggca cgagcgacat ggccgtcgtc accgcgtcgt cgctgcccga ttcggaactc gtgtcgcacc tgctcgggtc gaccttcagc attctgtgtg cgtcacccga ctacgtgaaa cggcacggcg tgccggcccg cccgcaggat ctcgccgcgc acgcgtgcct gacgctgtgc acgccggcgt ttccgacgca cgaatgggtg ctcgaagggc ccgaaggcgt cgagcagatc cacgtcgccg ggccggtgca gaccaatacc gccgaatcgc tcgcgctcgc gatccgcgac ggcatcggga tcggcatgct gccgctgtat tcggcgatcg acgcgctgcg cgacggcacg ctggtccgtg tgctgcccgc gcatatcctg cagaagatga acgtgtatgc gctgtacccg tcgcgccgtt tcgtcgatgc gaaggtgcgg acctgggtcg agatgttgcg tgcgcaagtg ccgggaatga tcgcgcgcga cgtcgagatg ctgaacgcga tcgaccgcga accgcaggcc gcctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa