BuceA.12800.p

LysR family regulatory protein

CENTER ID: BuceA.12800.p
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12800.p.A2.GE31871 Mature protein( BuceA.12800.p ) 23 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4021
RefSeq: YP_002232969.1
UniProt: B4EIT2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRNNLKGIDV FVAAVEASSF AQAAEKLHIT RSAVGKSIAR LEERLGVVLF HRTTRSQSLT DEGSLFYEHC LRAMDEIQAG EALLETGKWQ MKGRLRVSMP TLFGHLCVAP ILIGVAKAHP DLDLELSFSD RSVDLAEERF DLAVRIGTLP NSSGLVARAL AEHRMAFCAS PAYLSRYGEP QTWRELEQHK ALAYTRFGQV HRWPVLVDGE RVELQPAVRL LSDDLRALMD AALADLGVAW LPYWLVRDAL RGGALRELLP AQPGVVYPIH AVWPATPHLP LKVRAAVDAL REHLPARLAA VEARM
NT Sequence
ATGCGAAACA ACCTGAAAGG CATCGACGTG TTCGTCGCCG CGGTGGAAGC GAGCAGCTTC GCGCAGGCCG CCGAGAAGCT GCACATCACG CGCTCGGCCG TCGGCAAAAG CATCGCGCGC CTCGAGGAAC GGCTCGGCGT CGTGCTGTTT CACCGAACGA CGCGCAGCCA GAGCCTGACC GACGAGGGTT CGCTGTTCTA CGAGCATTGC CTGCGTGCGA TGGACGAGAT CCAGGCCGGC GAGGCGCTGC TCGAAACCGG CAAGTGGCAG ATGAAAGGGC GGCTGCGCGT GTCGATGCCG ACGCTGTTCG GCCATCTGTG CGTCGCGCCG ATCCTGATCG GCGTCGCGAA GGCGCACCCG GACCTCGATC TGGAGCTGTC CTTCAGCGAC CGGTCCGTCG ATCTGGCCGA GGAGCGCTTC GATCTCGCGG TGCGCATCGG CACGTTGCCG AACAGCAGCG GACTGGTCGC GCGAGCGCTG GCCGAGCACC GGATGGCGTT CTGCGCGTCG CCGGCTTACC TGAGCCGATA CGGCGAACCG CAAACCTGGC GGGAACTGGA GCAGCACAAG GCGCTGGCTT ATACGCGCTT TGGCCAGGTG CATCGCTGGC CCGTTCTCGT CGACGGCGAG CGCGTGGAAT TGCAGCCCGC CGTCCGGCTG CTGTCCGACG ACCTGCGTGC GTTGATGGAT GCCGCCCTTG CCGATCTCGG CGTCGCGTGG CTGCCGTACT GGCTGGTGCG CGACGCATTG CGCGGCGGCG CGTTGCGGGA GCTGCTGCCG GCGCAGCCGG GCGTCGTCTA CCCGATCCAC GCGGTGTGGC CGGCCACGCC GCACCTGCCG CTGAAAGTGC GGGCCGCCGT CGATGCGCTG CGGGAACACT TGCCCGCCCG GCTGGCCGCG GTCGAAGCGC GGATG
Details for BuceA.12800.p.A2.GE31871
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 89
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAEKLHIT RSAVGKSIAR LEERLGVVLF HRTTRSQSLT DEGSLFYEHC LRAMDEIQAG EALLETGKWQ MKGRLRVSMP TLFGHLCVAP ILIGVAKAHP DLDLELSFSD RSVDLAEERF DLAVRIGTLP NSSGLVARAL AEHRMAFCAS PAYLSRYGEP QTWRELEQHK ALAYTRFGQV HRWPVLVDGE RVELQPAVRL LSDDLRALMD AALXDLGVAW LPYWLXRDAL RGGALRELLX AQPGVXYPIH XXXARXRRTC XXKVRXPSXR CGXXXPPGWX AVXAXXX
Validated NT Sequence
cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gnnnnnnnnn tntnngnnan nngnnnannn naccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gccgccgaga agctgcacat cacgcgctcg gccgtcggca aaagcatcgc gcgcctcgag gaacggctcg gcgtcgtgct gtttcaccga acgacgcgca gccagagcct gaccgacgag ggttcgctgt tctacgagca ttgcctgcgt gcgatggacg agatccaggc cggcgaggcg ctgctcgaaa ccggcaagtg gcagatgaaa gggcggctgc gcgtgtcgat gccgacgctg ttcggccatc tgtgcgtcgc gccgatcctg atcggcgtcg cgaaggcgca cccggacctc gatctggagc tgtccttcag cgaccggtcc gtcgatctgg ccgaggagcg cttcgatctc gcggtgcgca tcggcacgtt gccgaacagc agcggactgg tcgcgcgagc gctggccgag caccggatgg cgttctgcgc gtcgccggct tacctgagcc gatacggcga accgcaaacc tggcgggaac tggagcagca caaggcgctg gcttatacgc gctttggcca ggtgcatcgc tggcccgttc tcgtcgacgg cgagcgcgtg gaattgcagc ccgccgtccg gctgctgtcc gacgacctgc gtgcgttgat ggatgccgcc cttgnngatc tcggcgtcgc gtggctgccg tactggctgg ngcgcgacgc attgcgcggc ggcgcgttgc gggagctgct gcnngcgcag ccgggcgtcn tctacccgat ccacgnnnnn gnggcccggn cacgncgcac ctgccnnntg aaagtgcggn ngccgtcnnt gcgctgcggg ancantngcc cncccggctg gnccgcggtc nnagcnnngn ngtnataaac agcnannaan nnnttcngca nncannnnnn tnnaggatnc nngnctnnnn nnnaaacnnn anngnannnn tnnannnnng cnngncnnnc caccng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAEKLHIT RSAVGKSIAR LEERLGVVLF HRTTRSQSLT DEGSLFYEHC LRAMDEIQAG EALLETGKWQ MKGRLRVSMP TLFGHLCVAP ILIGVAKAHP DLDLELSFSD RSVDLAEERF DLAVRIGTLP NSSGLVARAL AEHRMAFCAS PAYLSRYGEP QTWRELEQHK ALAYTRFGQV HRWPVLVDGE RVELQPAVRL LSDDLRALMD AALADLGVAW LPYWLVRDAL RGGALRELLP AQPGVVYPIH AVWPATPHLP LKVRAAVDAL REHLPARLAA VEARM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgccgag aagctgcaca tcacgcgctc ggccgtcggc aaaagcatcg cgcgcctcga ggaacggctc ggcgtcgtgc tgtttcaccg aacgacgcgc agccagagcc tgaccgacga gggttcgctg ttctacgagc attgcctgcg tgcgatggac gagatccagg ccggcgaggc gctgctcgaa accggcaagt ggcagatgaa agggcggctg cgcgtgtcga tgccgacgct gttcggccat ctgtgcgtcg cgccgatcct gatcggcgtc gcgaaggcgc acccggacct cgatctggag ctgtccttca gcgaccggtc cgtcgatctg gccgaggagc gcttcgatct cgcggtgcgc atcggcacgt tgccgaacag cagcggactg gtcgcgcgag cgctggccga gcaccggatg gcgttctgcg cgtcgccggc ttacctgagc cgatacggcg aaccgcaaac ctggcgggaa ctggagcagc acaaggcgct ggcttatacg cgctttggcc aggtgcatcg ctggcccgtt ctcgtcgacg gcgagcgcgt ggaattgcag cccgccgtcc ggctgctgtc cgacgacctg cgtgcgttga tggatgccgc ccttgccgat ctcggcgtcg cgtggctgcc gtactggctg gtgcgcgacg cattgcgcgg cggcgcgttg cgggagctgc tgccggcgca gccgggcgtc gtctacccga tccacgcggt gtggccggcc acgccgcacc tgccgctgaa agtgcgggcc gccgtcgatg cgctgcggga acacttgccc gcccggctgg ccgcggtcga agcgcggatg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa