SSGCID: Target Page

BuceA.12800.m

LysR family regulatory protein

CENTER ID:	BuceA.12800.m
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.12800.m.A2.GE31868	Mature protein( BuceA.12800.m )		25	301

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2204
RefSeq:	YP_002231247.1
UniProt:	B4ECR1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRFKQIETF AAVAAKGSLS AAAHAEGVAP AIIGRRLDAL EERLGVKLLV RTTRKLTLTF EGSAFLEDCQ RIINDMQNAE ASVSAGGVKA SGHLRISAPA GFGRRHVAPL VPEFSVGHPD VSVTLDLSDR MVDLVNEGFD CAVRLGELPD SSLVSLKLGE NRRVCVASPA YLARRGTPVT LAELARHNCL ALAANANQQR GWAFQEDDKV VSIRVNGTME CSDGAVLHEW CLAGHGLAWR SWWEVGEDIA AGRLVSVLDA FAAPPIGIHA VFPQRRHLPL RVRLFLDYLK HTYERPGYWG E

NT Sequence

ATGGACCGGT TCAAACAGAT CGAAACGTTC GCCGCGGTCG CGGCGAAAGG CAGCCTGTCG GCGGCGGCGC ATGCGGAAGG CGTCGCGCCG GCGATCATCG GGCGCCGCCT CGACGCGCTC GAGGAGCGGC TCGGCGTCAA GCTGCTGGTG CGCACGACGC GCAAGCTCAC GCTGACGTTC GAGGGCTCGG CATTCCTCGA GGATTGCCAG CGCATCATCA ACGACATGCA GAACGCGGAA GCGAGCGTGT CCGCCGGCGG CGTCAAGGCG AGCGGCCACC TGCGGATTTC CGCGCCGGCC GGCTTCGGCC GCCGGCACGT CGCGCCGCTC GTGCCCGAAT TCAGCGTCGG GCACCCCGAC GTGTCGGTCA CGCTCGACCT GTCCGACCGG ATGGTCGATC TCGTCAACGA AGGGTTCGAT TGCGCGGTGC GGCTCGGCGA GCTGCCCGAC TCGTCGCTGG TGTCGCTGAA GCTCGGCGAG AATCGCCGCG TGTGTGTCGC GTCTCCCGCA TATCTCGCGC GGCGCGGCAC GCCGGTCACG CTCGCGGAAC TGGCGCGGCA CAACTGCCTG GCCCTGGCGG CGAACGCGAA CCAGCAGCGC GGCTGGGCGT TCCAGGAGGA CGACAAGGTC GTGTCGATCC GCGTGAACGG CACGATGGAA TGCTCGGACG GCGCGGTGCT GCACGAGTGG TGCCTCGCAG GCCATGGCCT CGCGTGGCGC TCGTGGTGGG AAGTCGGCGA GGACATCGCG GCCGGGCGGC TCGTCAGCGT GCTCGACGCG TTCGCCGCGC CGCCGATCGG CATCCACGCG GTGTTTCCGC AGCGCCGGCA TCTGCCGCTG CGGGTGCGCC TGTTTCTCGA TTACCTGAAG CACACGTACG AGCGGCCCGG CTATTGGGGC GAA

Details for BuceA.12800.m.A2.GE31868

HARVESTED ON:	3/22/2011
SEQUENCED ON:	3/25/2011
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAEGVAPAI IGRRLDALEE RLGVKLLVRT TRKLTLTFEG SAFLEDCQRI INDMQNAEAS VSAGGVKASG HLRISAPAGF GRRHVAPLVP EFSVGHPDVS VTLDLSDRMV DLVNEGFDCA VRLGELPDSS LVSLKLGENR RVCVASPAYL ARRGTPVTLA ELARHNCLAL AANANQQRGW AFQEDDKVVS IRVNGTMECS DGAVLHEWCL AGHGLAWRSW WEVGEDIAAG RLVSVLDXXA APPIGIHAVF PQRRHLPLRV RLFLDYLKHT YXRPGYWGE

Validated NT Sequence

gnnnnnnnnn nnnnntannn gnnnnnntac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggcg gaaggcgtcg cgccggcgat catcgggcgc cgcctcgacg cgctcgagga gcggctcggc gtcaagctgc tggtgcgcac gacgcgcaag ctcacgctga cgttcgaggg ctcggcattc ctcgaggatt gccagcgcat catcaacgac atgcagaacg cggaagcgag cgtgtccgcc ggcggcgtca aggcgagcgg ccacctgcgg atttccgcgc cggccggctt cggccgccgg cacgtcgcgc cgctcgtgcc cgaattcagc gtcgggcacc ccgacgtgtc ggtcacgctc gacctgtccg accggatggt cgatctcgtc aacgaagggt tcgattgcgc ggtgcggctc ggcgagctgc ccgactcgtc gctggtgtcg ctgaagctcg gcgagaatcg ccgcgtgtgt gtcgcgtctc ccgcatatct cgcgcggcgc ggcacgccgg tcacgctcgc ggaactggcg cggcacaact gcctggccct ggcggcgaac gcgaaccagc agcgcggctg ggcgttccag gaggacgaca aggtcgtgtc gatccgcgtg aacggcacga tggaatgctc ggacggcgcg gtgctgcacg agtggtgcct cgcaggccat ggcctcgcgt ggcgctcgtg gtgggaagtc ggcgaggaca tcgcggccgg gcggctcgtc agcgtgctcg acnnnnnngc cgcgccgccg atcggcatcc acgcggtgtt tccgcagcgc cggcatctgc cgctgcgggt gcgcctgttt ctcgattacc tgaagcacac gtacgancgg cccggctatt ggggcgaata ataaacagca cgaacnagtt ctgcagccnn gcttctcgng nnnnnctgct acnaagncnn naagnanctg antnnctgct ncncnntna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggaaggc gtcgcgccgg cgatcatcgg gcgccgcctc gacgcgctcg aggagcggct cggcgtcaag ctgctggtgc gcacgacgcg caagctcacg ctgacgttcg agggctcggc attcctcgag gattgccagc gcatcatcaa cgacatgcag aacgcggaag cgagcgtgtc cgccggcggc gtcaaggcga gcggccacct gcggatttcc gcgccggccg gcttcggccg ccggcacgtc gcgccgctcg tgcccgaatt cagcgtcggg caccccgacg tgtcggtcac gctcgacctg tccgaccgga tggtcgatct cgtcaacgaa gggttcgatt gcgcggtgcg gctcggcgag ctgcccgact cgtcgctggt gtcgctgaag ctcggcgaga atcgccgcgt gtgtgtcgcg tctcccgcat atctcgcgcg gcgcggcacg ccggtcacgc tcgcggaact ggcgcggcac aactgcctgg ccctggcggc gaacgcgaac cagcagcgcg gctgggcgtt ccaggaggac gacaaggtcg tgtcgatccg cgtgaacggc acgatggaat gctcggacgg cgcggtgctg cacgagtggt gcctcgcagg ccatggcctc gcgtggcgct cgtggtggga agtcggcgag gacatcgcgg ccgggcggct cgtcagcgtg ctcgacgcgt tcgccgcgcc gccgatcggc atccacgcgg tgtttccgca gcgccggcat ctgccgctgc gggtgcgcct gtttctcgat tacctgaagc acacgtacga gcggcccggc tattggggcg aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa