SSGCID: Target Page

BuceA.12800.l

LysR family regulatory protein

CENTER ID:	BuceA.12800.l
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.12800.l.A1.GE31867	Full length( BuceA.12800.l )		1	321

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.12800.l.A1.PS01091	Full length( BuceA.12800.l )		1	321

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2114
RefSeq:	YP_002231163.1
UniProt:	B4EC43

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRFDAMQAF ARVVEAGSFT KAADTLHMSR TTVTQLVQQL EARLRVKLFN RTTRKVVVTA DGAAYYERVV RLLADMDDAE TSLSAASASP RGRLRVDVPS PFARLILIPA LPAFHARHPD IQLDMGVSDR VVDVIGENVD CVVRGGEPTD RSLVARHIGD LRLGVYASPA YLARAGTPSH PAELDDAHHR VVGFLWARTG KTLPFAMACD GEQVHVRGRY VLAVDDGNAY LAAGLAGLGI LWLPDYMAAA YRANGELVPL FAQWQLEPMP MYVAFPPNRH VSAKLRVFVD WVAELMTQHA PVATRRRGGQ GSRAMRQSSS I

NT Sequence

ATGGATCGAT TCGACGCAAT GCAGGCGTTC GCCCGCGTGG TGGAAGCCGG CAGCTTCACG AAGGCAGCCG ACACGCTGCA CATGAGCCGC ACCACCGTCA CGCAACTGGT GCAGCAGCTC GAAGCGCGGC TGCGCGTGAA GCTGTTCAAC CGCACGACCC GCAAGGTCGT CGTCACGGCC GACGGCGCCG CGTACTACGA ACGTGTCGTG CGGCTGCTGG CCGACATGGA CGATGCCGAA ACGAGCCTGT CCGCGGCGTC GGCGTCGCCG CGCGGGCGGC TGCGGGTGGA CGTGCCGAGC CCGTTCGCGC GCCTGATCCT GATCCCCGCG CTACCCGCGT TTCATGCGCG CCATCCGGAC ATCCAGCTCG ACATGGGCGT GAGCGACCGC GTGGTCGACG TGATCGGCGA GAACGTCGAT TGCGTCGTGC GCGGCGGCGA GCCGACCGAC CGCTCGCTGG TCGCGCGCCA CATCGGCGAC CTGCGGCTCG GCGTGTACGC ATCGCCCGCC TATCTCGCGC GCGCCGGTAC GCCGTCGCAT CCGGCCGAAC TGGACGACGC GCATCATCGC GTCGTCGGCT TTCTGTGGGC GCGCACCGGC AAGACGCTGC CGTTCGCGAT GGCATGCGAC GGCGAGCAGG TCCACGTGCG TGGGCGCTAC GTGCTCGCGG TCGACGACGG CAATGCGTAC CTCGCAGCCG GCCTCGCAGG GCTCGGCATC CTGTGGCTGC CCGACTACAT GGCCGCCGCG TATCGCGCGA ACGGCGAACT GGTGCCGCTG TTCGCGCAAT GGCAACTCGA ACCGATGCCG ATGTACGTCG CCTTCCCGCC GAACCGGCAC GTCAGCGCGA AGCTGCGCGT GTTCGTCGAC TGGGTGGCCG AGCTGATGAC GCAGCATGCG CCGGTCGCCA CCCGGCGGCG CGGCGGCCAG GGTTCGCGCG CGATGCGCCA ATCCTCGTCC ATC

Details for BuceA.12800.l.A1.GE31867

HARVESTED ON:	3/22/2011
SEQUENCED ON:	3/25/2011
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMDRFDAM QAFARVVEAG SFTKAADTLH MSRTTVTQLV QQLEARLRVK LFNRTTRKVV VTADGAAYYE RVVRLLADMD DAETSLSAAS ASPRGRLRVD VPSPFARLIL IPALPAFHAR HPDIQLDMGV SDRVVDVIGE NVDCVVRGGE PTDRSLVARH IGDLRLGVYA SPAYLARAGT PSHPAELDDA HHRVVGFLWA RTGKTLPFAM ACDGEQVHVR GRYVLAVDDG NAYLAAGLAG LGILWLPDYM AAAYRANGEL VPLFAQWQLE PMPMYVAFPP NRHVSAKLRV FVDWVAELMT QHAPVATRRR GGQGSRAMRQ SSSI

Validated NT Sequence

ttattagatg gacgaggatt ggcgcatcgc gcgcgaaccc tggccgccgc gccgccgggt ggcgaccggc gcatgctgcg tcatcagctc ggccacccag tcgacgaaca cgcgcagctt cgcgctgacg tgccggttcg gcgggaaggc gacgtacatc ggcatcggtt cgagttgcca ttgcgcgaac agcggcacca gttcgccgtt cgcgcgatac gcggcggcca tgtagtcggg cagccacagg atgccgagcc ctgcgaggcc ggctgcgagg tacgcattgc cgtcgtcgac cgcgagcacg tagcgcccac gcacgtggac ctgctcgccg tcgcatgcca tcgcgaacgg cagcgtcttg ccggtgcgcg cccacagaaa gccgacgacg cgatgatgcg cgtcgtccag ttcggccgga tgcgacggcg taccggcgcg cgcgagatag gcgggcgatg cgtacacgcc gagccgcagg tcgccgatgt ggcgcgcgac cagcgagcgg tcggtcggct cgccgccgcg cacgacgcaa tcgacgttct cgccgatcac gtcgaccacg cggtcgctca cgcccatgtc gagctggatg tccggatggc gcgcatgaaa cgcgggtagc gcggggatca ggatcaggcg cgcgaacggg ctcggcacgt ccacccgcag ccgcccgcgc ggcgacgccg acgccgcgga caggctcgtt tcggcatcgt ccatgtcggc cagcagccgc acgacacgtt cgtagtacgc ggcgccgtcg gccgtgacga cgaccttgcg ggtcgtgcgg ttgaacagct tcacgcgcag ccgcgcttcg agctgctgca ccagttgcgt gacggtggtg cggctcatgt gcagcgtgtc ggctgccttc gtgaagctgc cggcttccac cacgcgggcg aacgcctgca ttgcgtcgaa tcgatccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDRFDAMQA FARVVEAGSF TKAADTLHMS RTTVTQLVQQ LEARLRVKLF NRTTRKVVVT ADGAAYYERV VRLLADMDDA ETSLSAASAS PRGRLRVDVP SPFARLILIP ALPAFHARHP DIQLDMGVSD RVVDVIGENV DCVVRGGEPT DRSLVARHIG DLRLGVYASP AYLARAGTPS HPAELDDAHH RVVGFLWART GKTLPFAMAC DGEQVHVRGR YVLAVDDGNA YLAAGLAGLG ILWLPDYMAA AYRANGELVP LFAQWQLEPM PMYVAFPPNR HVSAKLRVFV DWVAELMTQH APVATRRRGG QGSRAMRQSS SI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatcgattc gacgcaatgc aggcgttcgc ccgcgtggtg gaagccggca gcttcacgaa ggcagccgac acgctgcaca tgagccgcac caccgtcacg caactggtgc agcagctcga agcgcggctg cgcgtgaagc tgttcaaccg cacgacccgc aaggtcgtcg tcacggccga cggcgccgcg tactacgaac gtgtcgtgcg gctgctggcc gacatggacg atgccgaaac gagcctgtcc gcggcgtcgg cgtcgccgcg cgggcggctg cgggtggacg tgccgagccc gttcgcgcgc ctgatcctga tccccgcgct acccgcgttt catgcgcgcc atccggacat ccagctcgac atgggcgtga gcgaccgcgt ggtcgacgtg atcggcgaga acgtcgattg cgtcgtgcgc ggcggcgagc cgaccgaccg ctcgctggtc gcgcgccaca tcggcgacct gcggctcggc gtgtacgcat cgcccgccta tctcgcgcgc gccggtacgc cgtcgcatcc ggccgaactg gacgacgcgc atcatcgcgt cgtcggcttt ctgtgggcgc gcaccggcaa gacgctgccg ttcgcgatgg catgcgacgg cgagcaggtc cacgtgcgtg ggcgctacgt gctcgcggtc gacgacggca atgcgtacct cgcagccggc ctcgcagggc tcggcatcct gtggctgccc gactacatgg ccgccgcgta tcgcgcgaac ggcgaactgg tgccgctgtt cgcgcaatgg caactcgaac cgatgccgat gtacgtcgcc ttcccgccga accggcacgt cagcgcgaag ctgcgcgtgt tcgtcgactg ggtggccgag ctgatgacgc agcatgcgcc ggtcgccacc cggcggcgcg gcggccaggg ttcgcgcgcg atgcgccaat cctcgtccat ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for BuceA.12800.l.A1.PS01091

PURIFICATION DATe:	5/19/2011
CONCENTRATION:	11.06mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATCGATTC GACGCAATGC AGGCGTTCGC CCGCGTGGTG GAAGCCGGCA GCTTCACGAA GGCAGCCGAC ACGCTGCACA TGAGCCGCAC CACCGTCACG CAACTGGTGC AGCAGCTCGA AGCGCGGCTG CGCGTGAAGC TGTTCAACCG CACGACCCGC AAGGTCGTCG TCACGGCCGA CGGCGCCGCG TACTACGAAC GTGTCGTGCG GCTGCTGGCC GACATGGACG ATGCCGAAAC GAGCCTGTCC GCGGCGTCGG CGTCGCCGCG CGGGCGGCTG CGGGTGGACG TGCCGAGCCC GTTCGCGCGC CTGATCCTGA TCCCCGCGCT ACCCGCGTTT CATGCGCGCC ATCCGGACAT CCAGCTCGAC ATGGGCGTGA GCGACCGCGT GGTCGACGTG ATCGGCGAGA ACGTCGATTG CGTCGTGCGC GGCGGCGAGC CGACCGACCG CTCGCTGGTC GCGCGCCACA TCGGCGACCT GCGGCTCGGC GTGTACGCAT CGCCCGCCTA TCTCGCGCGC GCCGGTACGC CGTCGCATCC GGCCGAACTG GACGACGCGC ATCATCGCGT CGTCGGCTTT CTGTGGGCGC GCACCGGCAA GACGCTGCCG TTCGCGATGG CATGCGACGG CGAGCAGGTC CACGTGCGTG GGCGCTACGT GCTCGCGGTC GACGACGGCA ATGCGTACCT CGCAGCCGGC CTCGCAGGGC TCGGCATCCT GTGGCTGCCC GACTACATGG CCGCCGCGTA TCGCGCGAAC GGCGAACTGG TGCCGCTGTT CGCGCAATGG CAACTCGAAC CGATGCCGAT GTACGTCGCC TTCCCGCCGA ACCGGCACGT CAGCGCGAAG CTGCGCGTGT TCGTCGACTG GGTGGCCGAG CTGATGACGC AGCATGCGCC GGTCGCCACC CGGCGGCGCG GCGGCCAGGG TTCGCGCGCG ATGCGCCAAT CCTCGTCCAT CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA