BuceA.12800.k

LysR family regulatory protein

CENTER ID: BuceA.12800.k
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12800.k.A1.GE31866 Full length( BuceA.12800.k ) 1 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.992
RefSeq: YP_002230100.1
UniProt: B4EBX6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRIQAMEVF TRVVDANSFT RAADTLAMPR ASVTTIIQNL EALLGVRLMH RTTRRLSLTP EGAAYYEHCM KIIAEIAEAD ASFQTGNRKP SGVLRVHMPS SLGRRIVLPS LSIFRQRYPD ITLELGLSDR YVDPVEEGID CMIRVGPLED SSMVARRIGM LKRVTCASPD YLARHGEPNE IADLVEHHAV NFRSSHGARA IPWVFMIDGK PFEVRMNGSV TVNDSDAYVT CGLEGFGMIQ PTLFMVLPNL LDGSLVEVLP DCNPKPKPIS IVYPHNRHLS QKVRVFADWI AEVFESTPAL EGGENWRGKL RAQTPDAQRG AVVA
NT Sequence
ATGGACCGAA TCCAGGCAAT GGAAGTTTTT ACCCGCGTGG TCGACGCGAA CAGCTTCACG CGGGCGGCCG ACACGCTCGC GATGCCGCGC GCGTCGGTGA CGACCATCAT CCAGAATCTC GAGGCGCTGC TCGGCGTGCG CCTGATGCAC CGGACGACGC GCCGGCTGTC GCTGACGCCG GAGGGCGCCG CGTACTACGA GCATTGCATG AAGATCATCG CGGAGATCGC CGAGGCCGAC GCGAGTTTCC AGACCGGCAA CCGCAAGCCG AGCGGCGTGC TGCGCGTCCA CATGCCGAGT TCGCTCGGGC GGCGCATCGT GCTGCCGTCG CTGTCGATCT TCCGGCAGCG CTATCCGGAC ATCACGCTCG AGCTGGGATT GTCCGACCGT TACGTCGATC CGGTCGAGGA AGGCATCGAC TGCATGATCC GGGTCGGCCC GCTCGAGGAT TCGTCGATGG TCGCGCGGCG CATCGGGATG CTCAAGCGCG TGACGTGCGC GTCGCCCGAT TACCTCGCCC GTCACGGCGA GCCGAACGAG ATTGCCGATC TCGTCGAACA TCATGCGGTG AATTTCCGGT CGAGCCATGG CGCGCGGGCG ATTCCGTGGG TGTTCATGAT CGACGGCAAG CCGTTCGAGG TGCGGATGAA CGGCAGCGTG ACGGTCAACG ACTCGGATGC GTACGTGACG TGCGGGCTCG AAGGCTTCGG GATGATCCAG CCGACGCTGT TCATGGTGCT GCCGAACCTG CTCGACGGAT CGCTCGTCGA GGTGCTGCCG GACTGCAATC CGAAGCCGAA ACCGATCTCG ATCGTCTATC CGCACAACCG GCATCTGTCG CAGAAGGTGC GCGTGTTCGC CGACTGGATC GCGGAGGTGT TCGAGTCGAC GCCGGCGCTG GAGGGCGGGG AGAACTGGCG GGGGAAGCTG CGGGCGCAGA CGCCGGATGC GCAGCGCGGC GCGGTGGTGG CG
Details for BuceA.12800.k.A1.GE31866
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MDRIQAMEVF TRVVDANSFT RAADTLAMPR ASVTTIIQNL EALLGVRLMH RTTRRLSLTP EGAAYYEHCM KIIAEIAEAD ASFQTGNRKP SGVLRVHMPS SLGRRIVLPS LSIFRQRYPD ITLELGLSDR YVDPVEEGID CMIRVGPLED SSMVARRIGM LKRVTCASPD YLARHGEPNE IADLVEHHAV NFRSSHGARA IPWVFMIDGK PFEVRMNGSV TVNDSDAYVT CGLEGFGMIQ PTLFMVLPNL LDGSLVEVLP DCNPKPKPIS IVYPHNRHLS QKVRVFADWI AEVFESTPAL EGGENWRGKL RAQTPDAQRG AVV
Validated NT Sequence
cgccaccacc gcgccgcgct gcgcatccgg cgtctgcgcc cgcagcttcc cccgccagtt ctccccgccc tccagcgccg gcgtcgactc gaacacctcc gcgatccagt cggcgaacac gcgcaccttc tgcgacagat gccggttgtg cggatagacg atcgagatcg gtttcggctt cggattgcag tccggcagca cctcgacgag cgatccgtcg agcaggttcg gcagcaccat gaacagcgtc ggctggatca tcccgaagcc ttcgagcccg cacgtcacgt acgcatccga gtcgttgacc gtcacgctgc cgttcatccg cacctcgaac ggcttgccgt cgatcatgaa cacccacgga atcgcccgcg cgccatggct cgaccggaaa ttcaccgcat gatgttcgac gagatcggca atctcgttcg gctcgccgtg acgggcgagg taatcgggcg acgcgcacgt cacgcgcttg agcatcccga tgcgccgcgc gaccatcgac gaatcctcga gcgggccgac ccggatcatg cagtcgatgc cttcctcgac cggatcgacg taacggtcgg acaatcccag ctcgagcgtg atgtccggat agcgctgccg gaagatcgac agcgacggca gcacgatgcg ccgcccgagc gaactcggca tgtggacgcg cagcacgccg ctcggcttgc ggttgccggt ctggaaactc gcgtcggcct cggcgatctc cgcgatgatc ttcatgcaat gctcgtagta cgcggcgccc tccggcgtca gcgacagccg gcgcgtcgtc cggtgcatca ggcgcacgcc gagcagcgcc tcgagattct ggatgatggt cgtcaccgac gcgcgcggca tcgcgagcgt gtcggccgcc cgcgtgaagc tgttcgcgtc gaccacgcgg gtaaaaactt ccattgcctg gattcggtcc atcgaaccag gaccctgggt ctgagcttcc agggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRIQAMEV FTRVVDANSF TRAADTLAMP RASVTTIIQN LEALLGVRLM HRTTRRLSLT PEGAAYYEHC MKIIAEIAEA DASFQTGNRK PSGVLRVHMP SSLGRRIVLP SLSIFRQRYP DITLELGLSD RYVDPVEEGI DCMIRVGPLE DSSMVARRIG MLKRVTCASP DYLARHGEPN EIADLVEHHA VNFRSSHGAR AIPWVFMIDG KPFEVRMNGS VTVNDSDAYV TCGLEGFGMI QPTLFMVLPN LLDGSLVEVL PDCNPKPKPI SIVYPHNRHL SQKVRVFADW IAEVFESTPA LEGGENWRGK LRAQTPDAQR GAVVA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccgaatc caggcaatgg aagtttttac ccgcgtggtc gacgcgaaca gcttcacgcg ggcggccgac acgctcgcga tgccgcgcgc gtcggtgacg accatcatcc agaatctcga ggcgctgctc ggcgtgcgcc tgatgcaccg gacgacgcgc cggctgtcgc tgacgccgga gggcgccgcg tactacgagc attgcatgaa gatcatcgcg gagatcgccg aggccgacgc gagtttccag accggcaacc gcaagccgag cggcgtgctg cgcgtccaca tgccgagttc gctcgggcgg cgcatcgtgc tgccgtcgct gtcgatcttc cggcagcgct atccggacat cacgctcgag ctgggattgt ccgaccgtta cgtcgatccg gtcgaggaag gcatcgactg catgatccgg gtcggcccgc tcgaggattc gtcgatggtc gcgcggcgca tcgggatgct caagcgcgtg acgtgcgcgt cgcccgatta cctcgcccgt cacggcgagc cgaacgagat tgccgatctc gtcgaacatc atgcggtgaa tttccggtcg agccatggcg cgcgggcgat tccgtgggtg ttcatgatcg acggcaagcc gttcgaggtg cggatgaacg gcagcgtgac ggtcaacgac tcggatgcgt acgtgacgtg cgggctcgaa ggcttcggga tgatccagcc gacgctgttc atggtgctgc cgaacctgct cgacggatcg ctcgtcgagg tgctgccgga ctgcaatccg aagccgaaac cgatctcgat cgtctatccg cacaaccggc atctgtcgca gaaggtgcgc gtgttcgccg actggatcgc ggaggtgttc gagtcgacgc cggcgctgga gggcggggag aactggcggg ggaagctgcg ggcgcagacg ccggatgcgc agcgcggcgc ggtggtggcg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa