BuceA.12800.j

LysR family regulatory protein

CENTER ID: BuceA.12800.j
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12800.j.A1.GE31865 Full length( BuceA.12800.j ) 1 295
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.987
RefSeq: YP_002230095.1
UniProt: B4EBX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDELLPMRLF VKVAELRSFA RAATELNISN SSATRLVMSI EDRLGARLLN RTTRSLSLTD AGHLYLAQIR HVIDEIDRVE ETIASMHHEP IGSLKIAAPV MFGMHVLAPA IDAFKMRHPA IVPEVTIVDR HVDLVSEGFD VGLLLSQHIS NNTLVKRPLT RLKQIVCAAP HYVDRHGAPA HPHDLASHAC LSFHADFMGE YLSFDHDQTR ITVRPNKSAS SNNVGLIRNW ALSGMGIAVL PDFLVQSNLD DGQLLALLPG YRLETLEMNV AYPSRRHFPR KARLFVDHLI DHFVG
NT Sequence
ATGGACGAAC TCCTACCGAT GCGACTATTC GTCAAGGTCG CCGAACTTCG TAGTTTCGCG CGCGCCGCCA CGGAACTGAA CATCTCCAAT TCATCGGCCA CCCGCCTCGT CATGAGCATC GAGGACCGGC TTGGCGCCCG CCTGCTGAAT CGCACCACCC GCAGCCTGTC GCTGACCGAC GCAGGCCACC TCTATCTGGC GCAGATCCGT CACGTGATCG ACGAAATCGA CCGGGTCGAG GAAACGATCG CGTCGATGCA TCACGAACCG ATCGGATCGC TGAAAATCGC CGCGCCGGTC ATGTTCGGCA TGCACGTGCT CGCGCCGGCC ATCGACGCGT TCAAGATGCG CCACCCGGCC ATCGTGCCGG AGGTCACGAT CGTCGATCGT CACGTCGACC TCGTGTCGGA GGGCTTCGAC GTCGGGCTGC TGCTGTCCCA GCACATCAGC AACAACACGC TGGTGAAGCG CCCGCTCACG CGGCTCAAGC AGATCGTCTG CGCCGCACCG CACTATGTCG ACCGGCATGG CGCGCCCGCG CATCCGCACG ACCTCGCGTC GCACGCGTGC CTGTCGTTCC ATGCCGACTT CATGGGCGAA TACCTGTCGT TCGATCACGA CCAGACGCGC ATCACCGTGC GCCCGAACAA GTCCGCGTCG TCGAACAACG TCGGGCTGAT CCGCAACTGG GCGCTGTCCG GCATGGGCAT CGCGGTCCTG CCGGACTTCC TCGTCCAGTC GAATCTCGAC GACGGCCAAC TGCTCGCGCT GCTGCCCGGC TATCGGCTCG AGACGCTCGA GATGAACGTC GCATACCCGA GCCGCCGTCA CTTTCCGCGC AAGGCCCGGC TTTTCGTCGA TCACCTGATC GACCATTTCG TCGGT
Details for BuceA.12800.j.A1.GE31865
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHXHHMG TLEAQTQGPG SMDELLPMRL FVKVAELRSF ARAATELNIS NSSATRLVMS IEDRLGARLL NRTTRSLSLT DAGHLYLAQI RHVIDEIDRV EETIASMHHE PIGSLKIAAP VMFGMHVLAP AIDAFKMRHP AIVPEVTIVD RHVDLVSEGF DVGLLLSQHI SNNTLVKRPL TRLKQIVCAA PHYVDRHGAP AHPHDLASHA CLSFHADFMG EYLSFDHDQT RITVRPNKSA SSNNVXADPQ LGAVPAWASR SCRDFXRPVE XXTXXXXFXA AARLSGSRRS R
Validated NT Sequence
gnnnnnnnnn nnnnnnannn gtaccatggc tcatcaccat naccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgaactc ctaccgatgc gactattcgt caaggtcgcc gaacttcgta gtttcgcgcg cgccgccacg gaactgaaca tctccaattc atcggccacc cgcctcgtca tgagcatcga ggaccggctt ggcgcccgcc tgctgaatcg caccacccgc agcctgtcgc tgaccgacgc aggccacctc tatctggcgc agatccgtca cgtgatcgac gaaatcgacc gggtcgagga aacgatcgcg tcgatgcatc acgaaccgat cggatcgctg aaaatcgccg cgccggtcat gttcggcatg cacgtgctcg cgccggccat cgacgcgttc aagatgcgcc acccggccat cgtgccggag gtcacgatcg tcgatcgtca cgtcgacctc gtgtcggagg gcttcgacgt cgggctgctg ctgtcccagc acatcagcaa caacacgctg gtgaagcgcc cgctcacgcg gctcaagcag atcgtctgcg ccgcaccgca ctatgtcgac cggcatggcg cgcccgcgca tccgcacgac ctcgcgtcgc acgcgtgcct gtcgttccat gccgacttca tgggcgaata cctgtcgttc gatcacgacc agacgcgcat caccgtgcgc ccgaacaagt ccgcgtcgtc gaacaacgtc ngggctgatc cgcaactggg cgctgtcccg gcatgggcat cgcggtcctg ccgggacttt cntcgtccag tcgaanncnn nacgannnnn nactgnttcn nngctgctgc ccggctatcg ggctcgagac gctcgagatg aacgtcgcat accccgagcc gccgtcactt tccgcgcaag gnccgggctt ttcgtcgatc nncctgatcg accattttcg tcgggttaat aaancnagca cgaacaangt tcnngnannn aagcttnnnc naggatnccg gctggctaan nnaaagcccn gaaagganng cnnnnngttg ggcnggctnn gccnncnnnn ga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDELLPMRL FVKVAELRSF ARAATELNIS NSSATRLVMS IEDRLGARLL NRTTRSLSLT DAGHLYLAQI RHVIDEIDRV EETIASMHHE PIGSLKIAAP VMFGMHVLAP AIDAFKMRHP AIVPEVTIVD RHVDLVSEGF DVGLLLSQHI SNNTLVKRPL TRLKQIVCAA PHYVDRHGAP AHPHDLASHA CLSFHADFMG EYLSFDHDQT RITVRPNKSA SSNNVGLIRN WALSGMGIAV LPDFLVQSNL DDGQLLALLP GYRLETLEMN VAYPSRRHFP RKARLFVDHL IDHFVG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgaactc ctaccgatgc gactattcgt caaggtcgcc gaacttcgta gtttcgcgcg cgccgccacg gaactgaaca tctccaattc atcggccacc cgcctcgtca tgagcatcga ggaccggctt ggcgcccgcc tgctgaatcg caccacccgc agcctgtcgc tgaccgacgc aggccacctc tatctggcgc agatccgtca cgtgatcgac gaaatcgacc gggtcgagga aacgatcgcg tcgatgcatc acgaaccgat cggatcgctg aaaatcgccg cgccggtcat gttcggcatg cacgtgctcg cgccggccat cgacgcgttc aagatgcgcc acccggccat cgtgccggag gtcacgatcg tcgatcgtca cgtcgacctc gtgtcggagg gcttcgacgt cgggctgctg ctgtcccagc acatcagcaa caacacgctg gtgaagcgcc cgctcacgcg gctcaagcag atcgtctgcg ccgcaccgca ctatgtcgac cggcatggcg cgcccgcgca tccgcacgac ctcgcgtcgc acgcgtgcct gtcgttccat gccgacttca tgggcgaata cctgtcgttc gatcacgacc agacgcgcat caccgtgcgc ccgaacaagt ccgcgtcgtc gaacaacgtc gggctgatcc gcaactgggc gctgtccggc atgggcatcg cggtcctgcc ggacttcctc gtccagtcga atctcgacga cggccaactg ctcgcgctgc tgcccggcta tcggctcgag acgctcgaga tgaacgtcgc atacccgagc cgccgtcact ttccgcgcaa ggcccggctt ttcgtcgatc acctgatcga ccatttcgtc ggttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa