SSGCID: Target Page

BuceA.12800.h

LysR family regulatory protein

CENTER ID:	BuceA.12800.h
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.12800.h.A1.GE31863	Full length( BuceA.12800.h )		1	320

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.654
RefSeq:	YP_002229785.1
UniProt:	B4E948

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTLVSMNVF RYVVEVGSFV GAAERMQMSA AMASKHVMHL EQQLGARLLH RTTRRVAPTE AGREYYERLV QALSELDEAG QAVGAASVVP QGRLRVTSLS AFGLRHVMQA VTDYAGRFPD VTVDMTLSDR VVDLIEEGYD VAVRAAPNGL KSSSLVARQI ATAHILLVAS PEYLEKRGTP QTIADLANHN YLRRDSNSTM LDSLVIDAAA ASRVSLNGNL IVNHLEGLRA AVLAGAGIAL LGTEVVGDDI ESGRLVPVLL DSVPPHEAPI YAVYASRRHV SAKVRSFVDF LAARFEGQSL CPSIEARLRV LPITRMKRAV

NT Sequence

ATGGATACCC TCGTCAGCAT GAATGTGTTT CGCTACGTCG TCGAAGTGGG CAGCTTCGTC GGCGCGGCGG AGCGCATGCA GATGTCGGCG GCGATGGCGA GCAAGCACGT GATGCACCTC GAGCAGCAGC TCGGCGCGCG GCTGCTGCAT CGCACGACAC GACGCGTCGC GCCCACCGAG GCCGGACGTG AATACTATGA GCGCCTCGTG CAGGCGCTGT CCGAACTCGA CGAGGCCGGT CAGGCCGTCG GCGCCGCGAG CGTGGTGCCG CAGGGCCGGC TGCGCGTGAC GTCGCTGTCC GCGTTCGGGC TGCGGCACGT GATGCAGGCC GTCACCGATT ACGCGGGTCG GTTCCCGGAC GTGACGGTCG ACATGACGCT CTCCGATCGC GTGGTCGACC TGATCGAGGA AGGCTACGAC GTCGCGGTGC GCGCGGCGCC GAACGGCCTG AAATCGTCGT CGCTGGTCGC GCGCCAGATC GCGACCGCGC ACATCCTGCT GGTCGCGTCG CCCGAGTATC TCGAGAAGCG TGGCACGCCG CAGACGATCG CCGATCTCGC GAACCACAAC TACCTGCGGC GCGATTCGAA TTCGACGATG CTCGATTCGC TGGTGATCGA CGCGGCCGCC GCGTCGCGCG TGAGCCTGAA CGGCAACCTG ATCGTGAACC ATCTCGAAGG GCTGCGCGCG GCCGTGCTCG CCGGCGCGGG CATCGCGCTG CTCGGCACCG AAGTGGTCGG CGACGACATC GAGTCGGGCC GGCTCGTGCC GGTGCTGCTC GATTCGGTGC CGCCGCACGA GGCGCCGATC TACGCGGTGT ACGCGAGCCG TCGTCACGTG TCCGCGAAGG TGCGCTCGTT CGTCGACTTC CTTGCCGCGC GCTTCGAAGG GCAGTCGTTG TGCCCGTCGA TCGAAGCGCG TCTGCGCGTG CTGCCGATCA CGCGGATGAA GCGCGCGGTC

Details for BuceA.12800.h.A1.GE31863

HARVESTED ON:	3/22/2011
SEQUENCED ON:	3/25/2011
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ttattagacc gcgcgcttca tccgcgtgat cggcagcacg cgcagacgcg cttcgatcga cgggcacaac gactgccctt cgaagcgcgc ggcaaggaag tcgacgaacg agcgcacctt cgcggacacg tgacgacggc tcgcgtacac cgcgtagatc ggcgcctcgt gcggcggcac cgaatcgagc agcaccggca cgagccggcc cgactcgatg tcgtcgccga ccacttcggt gccgagcagc gcgatgcccg cgccggcgag cacggccgcg cgcagccctt cgagatggtt cacgatcagg ttgccgttca ggctcacgcg cgacgcggcg gccgcgtcga tcaccagcga atcgagcatc gtcgaattcg aatcgcgccg caggtagttg tggttcgcga gatcggcgat cgtctgcggc gtgccacgct tctcgagata ctcgggcgac gcgaccagca ggatgtgcgc ggtcgcgatc tggcgcgcga ccagcgacga cgatttcagg ccgttcggcg ccgcgcgcac cgcgacgtcg tagccttcct cgatcaggtc gaccacgcga tcggagagcg tcatgtcgac cgtcacgtcc gggaaccgac ccgcgtaatc ggtgacggcc tgcatcacgt gccgcagccc gaacgcggac agcgacgtca cgcgcagccg gccctgcggc accacgctcg cggcgccgac ggcctgaccg gcctcgtcga gttcggacag cgcctgcacg aggcgctcat agtattcacg tccggcctcg gtgggcgcga cgcgtcgtgt cgtgcgatgc agcagccgcg cgccgagctg ctgctcgagg tgcatcacgt gcttgctcgc catcgccgcc gacatctgca tgcgctccgc cgcgccgacg aagctgccca cttcgacgac gtagcgaaac acattcatgc tgacgagggt atccatcgaa ccaggaccct gggtctgagc ttccagggta cc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDTLVSMNV FRYVVEVGSF VGAAERMQMS AAMASKHVMH LEQQLGARLL HRTTRRVAPT EAGREYYERL VQALSELDEA GQAVGAASVV PQGRLRVTSL SAFGLRHVMQ AVTDYAGRFP DVTVDMTLSD RVVDLIEEGY DVAVRAAPNG LKSSSLVARQ IATAHILLVA SPEYLEKRGT PQTIADLANH NYLRRDSNST MLDSLVIDAA AASRVSLNGN LIVNHLEGLR AAVLAGAGIA LLGTEVVGDD IESGRLVPVL LDSVPPHEAP IYAVYASRRH VSAKVRSFVD FLAARFEGQS LCPSIEARLR VLPITRMKRA V

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggataccctc gtcagcatga atgtgtttcg ctacgtcgtc gaagtgggca gcttcgtcgg cgcggcggag cgcatgcaga tgtcggcggc gatggcgagc aagcacgtga tgcacctcga gcagcagctc ggcgcgcggc tgctgcatcg cacgacacga cgcgtcgcgc ccaccgaggc cggacgtgaa tactatgagc gcctcgtgca ggcgctgtcc gaactcgacg aggccggtca ggccgtcggc gccgcgagcg tggtgccgca gggccggctg cgcgtgacgt cgctgtccgc gttcgggctg cggcacgtga tgcaggccgt caccgattac gcgggtcggt tcccggacgt gacggtcgac atgacgctct ccgatcgcgt ggtcgacctg atcgaggaag gctacgacgt cgcggtgcgc gcggcgccga acggcctgaa atcgtcgtcg ctggtcgcgc gccagatcgc gaccgcgcac atcctgctgg tcgcgtcgcc cgagtatctc gagaagcgtg gcacgccgca gacgatcgcc gatctcgcga accacaacta cctgcggcgc gattcgaatt cgacgatgct cgattcgctg gtgatcgacg cggccgccgc gtcgcgcgtg agcctgaacg gcaacctgat cgtgaaccat ctcgaagggc tgcgcgcggc cgtgctcgcc ggcgcgggca tcgcgctgct cggcaccgaa gtggtcggcg acgacatcga gtcgggccgg ctcgtgccgg tgctgctcga ttcggtgccg ccgcacgagg cgccgatcta cgcggtgtac gcgagccgtc gtcacgtgtc cgcgaaggtg cgctcgttcg tcgacttcct tgccgcgcgc ttcgaagggc agtcgttgtg cccgtcgatc gaagcgcgtc tgcgcgtgct gccgatcacg cggatgaagc gcgcggtcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a